Improved high-performance liquid chromatography-DAD method for the simultaneous analysis of quercetin and kaempferol in the stems of Cissus quadrangularis Linn

Czasopismo : Acta Chromatographica
Tytuł artykułu : Improved high-performance liquid chromatography-DAD method for the simultaneous analysis of quercetin and kaempferol in the stems of Cissus quadrangularis Linn

Autorzy :
Turło, J.
Medical University of Warsaw Department of Drug Technology 1 Banacha Street 02-097 Warsaw Poland Medical University of Warsaw Faculty of Pharmacy 1 Banacha Street 02-097 Warsaw Poland, jadwiga.turlo@wum.edu.p,
Komsta, Ł.
Medical University of Lublin Chair and Department of Medicinal Chemistry, Faculty of Pharmacy Jaczewskiego 4 20-090 Lublin Poland Medical University of Lublin Chair and Department of Pharmaceutical Botany, Faculty of Pharmacy Chodźki 1 20-093 Lublin Pola, lukasz.komsta@am.lublin.pl,
Vasta, J. D.
Lafayette College Department of Chemistry Easton PA 18042-1782 USA Lafayette College Department of Biology Easton PA 18042-1782 USA, shermaj@mail.lafayette.edu,
Sajewicz, M.
Silesian University Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland, kowalska@us.edu.pl,
Raju, Ch. B. V. N.
Aurobindo Pharma Limited Srikakulam Dist. Pydibhimavaram 532 409 India Andhra University Department of Inorganic and Analytical Chemistry Visakhapatnam 530 003 India, chbvn_5@yahoo.co.in,
Shende, P.
NMIMS University School of Pharmacy and Technology Management V.L. Mehta Road, Vile Parle (W) Mumbai 400056 India, shendepravin94@yahoo.co.in,
Dhaneshwar, S. R.
Bharati Vidyapeeth University, Poona College of Pharmacy Department of Pharmaceutical Chemistry Pune 411038 India, sunil.dhaneshwar@gmail.com,
Thakur, A. K.
University Institute of Chemical Technology 1A-286, Medicinal Natural Products Research Laboratory, Pharmaceutical Division Nathalal Parikh Marg, Matunga Mumbai 400 019 Maharastra India Pharmanza Herbal Pvt. Ltd Plot no 214, Near Vadadia Patiya, Borsad-T, achalthakur@gmail.com,
Abstrakty : A simple, rapid, and specific reversed-phase HPLC method with DAD detection has been used for analysis of two flavonoids, quercetin and kaempferol, in Cissus quadrangularis Linn. The flavonoids were well resolved within 10 min, and quantification was achieved, on an endcapped C 18 column at 370 nm with acetonitrile-phosphate buffer (pH 3.4, adjusted with glacial acetic acid) 60:40 (v/v) as isocratic mobile phase at a flow rate of 1.0 mL min-1. The method was validated for limit of detection, limit of quantification, linearity, precision, accuracy, and recovery. Linearity was demonstrated over the range 0.75 to 10 µg mL-1for quercetin and 1.0 to 10 µg mL-1for kaempferol with good correlation coefficients ( r 2 > 0.998). Detection limits were 0.075 and 0.10 µg mL-1for quercetin and kaempferol respectively. Recovery was 98.0-105.3% for quercetin and 95.0-101.1% for kaempferol. The method was successfully applied to analysis of two flavonoids in four samples of Cissus quadrangularis Linn.

Słowa kluczowe : quantitative HPLC, quercetin, kaempferol, Cissus quadrangularis Linn,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2009
Numer : Vol. 21, no. 1
Strony : 95 – 103
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DOI :
Cytuj : Turło, J. ,Komsta, Ł. ,Vasta, J. D. ,Sajewicz, M. ,Raju, Ch. B. V. N. ,Shende, P. ,Dhaneshwar, S. R. ,Thakur, A. K. , Improved high-performance liquid chromatography-DAD method for the simultaneous analysis of quercetin and kaempferol in the stems of Cissus quadrangularis Linn. Acta Chromatographica Vol. 21, no. 1/2009
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