Journal : Acta Chromatographica
Article : Influence of sample preparation methods on the quantitation of selected tropane alkaloids from herb of Datura innoxia mill. by HPTLC

Authors :
Fatemi, M. H.
University of Mazandaran Laboratory of Chemometrics, Faculty of Chemistry Babolsar Iran, mhfatemi@umz.ac.ir,
Elyasi, M.
University of Mazandaran Laboratory of Chemometrics, Faculty of Chemistry Babolsar Iran,
Sowa, I.
Medical University of Lublin Department of Analytical Chemistry Chodźki 4a 20-093 Lublin Poland, i.sowa@umlub.pl,
Zajdel, D.
Medical University of Lublin Department of Analytical Chemistry Chodźki 4a 20-093 Lublin Poland,
Kocjan, R.
Medical University of Lublin Department of Analytical Chemistry Chodźki 4a 20-093 Lublin Poland,
Pizoń, M.
Medical University of Lublin Department of Analytical Chemistry Chodźki 4a 20-093 Lublin Poland,
Wójciak-Kosior, M.
Medical University of Lublin Department of Analytical Chemistry Chodźki 4a 20-093 Lublin Poland,
Hadad, G. M.
Suez Canal University Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Ismailia 41522 Egypt, ghhadad@hotmail.com,
Abdel-Salam, R. A.
Suez Canal University Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Ismailia 41522 Egypt, dr_rasalam@yahoo.com,
Abdel-Hameed, E. A.
Suez Canal University Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Ismailia 41522 Egypt,
Liu, W.-Y.
China Pharmaceutical University Department of Pharmaceutical Analysis 24 Tongjiaxiang Nanjing 210009 China, liuwenyuan8506@163.com,
Xie, S.-L.
China Pharmaceutical University Department of Pharmaceutical Analysis 24 Tongjiaxiang Nanjing 210009 China,
Xu, X. Z.
China Pharmaceutical University Department of Pharmaceutical Analysis 24 Tongjiaxiang Nanjing 210009 China,
Wu, C. Y.
China Pharmaceutical University Department of Pharmaceutical Analysis 24 Tongjiaxiang Nanjing 210009 China,
Feng, F.
China Pharmaceutical University Department of Phytochemistry 24 Tongjiaxiang Nanjing 210009 China,
Maślanka, A.
Jagiellonian University Medical College, Pharmaceutical Faculty, Department of Inorganic and Analytical Chemistry Medyczna 9 30-688 Krakow Poland,
Hubicka, U.
Jagiellonian University Medical College, Pharmaceutical Faculty, Department of Inorganic and Analytical Chemistry Medyczna 9 30-688 Krakow Poland,
Krzek, J.
Jagiellonian University Medical College, Pharmaceutical Faculty, Department of Inorganic and Analytical Chemistry Medyczna 9 30-688 Krakow Poland, jankrzek@cm-uj.krakow.pl,
Walczak, M.
Jagiellonian University Medical College, Pharmaceutical Faculty, Department of Pharmacokinetics and Physical Pharmacy Medyczna 9 30-688 Krakow Poland,
Izworski, G.
Jagiellonian University Medical College, Pharmaceutical Faculty, Department of Inorganic and Analytical Chemistry Medyczna 9 30-688 Krakow Poland,
Suchy, D.
Medical University of Silesia Department of Internal Medicine and Clinical Pharmacology Medyków 18 PL 40752 Katowice Poland, dariuszsuchy@gmail.com,
Łabuzek, K.
Medical University of Silesia Department of Internal Medicine and Clinical Pharmacology Medyków 18 PL 40752 Katowice Poland,
Pierzchała, O.
Medical University of Silesia Department of Internal Medicine and Clinical Pharmacology Medyków 18 PL 40752 Katowice Poland,
Okopień, B.
Medical University of Silesia Department of Internal Medicine and Clinical Pharmacology Medyków 18 PL 40752 Katowice Poland,
Vamsi Krishna, M.
Alliance Institute of Advanced Pharmaceutical and Health Sciences Ameerpet, Hyderabad 500038 Andhra Pradesh India, marothu_vamsi@rediffmail.com,
Dash, R. N.
Alliance Institute of Advanced Pharmaceutical and Health Sciences Ameerpet, Hyderabad 500038 Andhra Pradesh India,
Jalachandra Reddy, B.
Alliance Institute of Advanced Pharmaceutical and Health Sciences Ameerpet, Hyderabad 500038 Andhra Pradesh India,
Venugopal, P.
Alliance Institute of Advanced Pharmaceutical and Health Sciences Ameerpet, Hyderabad 500038 Andhra Pradesh India,
Sandeep, P.
Alliance Institute of Advanced Pharmaceutical and Health Sciences Ameerpet, Hyderabad 500038 Andhra Pradesh India,
Madhavi, G.
Alliance Institute of Advanced Pharmaceutical and Health Sciences Ameerpet, Hyderabad 500038 Andhra Pradesh India,
Raghavi, K.
K.V.S.R Siddhartha College of Pharmaceutical Sciences Vijayawada 520010 AP India,
Sindhura, M.
K.V.S.R Siddhartha College of Pharmaceutical Sciences Vijayawada 520010 AP India,
Prashanthi, R.
K.V.S.R Siddhartha College of Pharmaceutical Sciences Vijayawada 520010 AP India,
Nalluri, B. N.
K.V.S.R Siddhartha College of Pharmaceutical Sciences Vijayawada 520010 AP India, buchinalluri@yahoo.com,
Jain, P. S.
R. C. Patel Institute of Pharmaceutical Education and Research Karwand Naka, Shirpur, Dist. Dhule 425 405 (M. S.) India, pritash79@yahoo.com,
Patel, M. K.
R. C. Patel Institute of Pharmaceutical Education and Research Karwand Naka, Shirpur, Dist. Dhule 425 405 (M. S.) India,
Surana, S. J.
R. C. Patel Institute of Pharmaceutical Education and Research Karwand Naka, Shirpur, Dist. Dhule 425 405 (M. S.) India,
Oniszczuk, A.
Medical University in Lublin Department of Inorganic Chemistry 4A Chodzki Street 20-093 Lublin Poland,
Waksmundzka-Hajnos, M.
Medical University in Lublin Department of Inorganic Chemistry 4A Chodzki Street 20-093 Lublin Poland, monika.hajnos@am.lublin.pl,
Gadzikowska, M.
Medical University in Lublin Department of Inorganic Chemistry 4A Chodzki Street 20-093 Lublin Poland,
Podgórski, R.
Medical University in Lublin Department of Inorganic Chemistry 4A Chodzki Street 20-093 Lublin Poland,
Oniszczuk, T.
University of Life Sciences in Lublin Department of Food Process Engineering Doświadczalna 44 20-236 Lublin Poland,
Abstract : The aim of our paper was the examination of various procedures of extraction and purification methods on the yield of atropine and scopolamine in Datura innoxia extracts determined by high-performance thin-layer chromatography (HPTLC). In our experiments, the following extraction methods were used: percolation, ultrasound-assisted extraction (USAE) at various temperatures, and microwave-assisted extraction (MASE). The highest extraction yields of atropine and scopolamine were obtained by ultrasound-assisted extraction method and aqueous acetic acid or aqueous tartaric acid used as extractants. Crude extracts of D. innoxia herb were purified by use of various methods: solid-phase extraction-strong cation exchanger (SPE-SCX), SPE-C18, and liquid-liquid extraction (LLE). The best results of purification with satisfactory %RSD values were obtained by liquid-liquid extraction by use of dichloromethane from aqueous crude alkaloid extract alkalized to pH 12. HPTLC was performed on pre-coated plates Kieselgel 60 F254 in horizontal DS chambers by use of mobile phase: methanol-acetone-aqueous ammonia (5:4.5:0.5). The alkaloids’ content was determined in extracts by use of calibration curve method for atropine and scopolamine. Calibration curves were linear in range of 3.0–30 μg for scopolamine (R = 0.9980) and 2.6–26 μg for atropine (R = 0.9923). Limits of detection (LOD) and limits of quantification (LOQ) were 1.12 μg and 3.42 μg per spot for scopolamine and 0.89 μg and 2.82 μg per spot for atropine.

Keywords : tropane alkaloids, Datura innoxia, Soxhlet extraction, ultrasonification, microwave-assisted solvent extraction, LLE, SPE,
Publishing house : University of Silesia in Katowice
Publication date : 2013
Number : Vol. 25, no. 3
Page : 545 – 554

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DOI :
Qute : Fatemi, M. H. ,Elyasi, M. ,Sowa, I. ,Zajdel, D. ,Kocjan, R. ,Pizoń, M. ,Wójciak-Kosior, M. ,Hadad, G. M. ,Abdel-Salam, R. A. ,Abdel-Hameed, E. A. ,Liu, W.-Y. ,Xie, S.-L. ,Xu, X. Z. ,Wu, C. Y. ,Feng, F. ,Maślanka, A. ,Hubicka, U. ,Krzek, J. ,Walczak, M. ,Izworski, G. ,Suchy, D. ,Łabuzek, K. ,Pierzchała, O. ,Okopień, B. ,Vamsi Krishna, M. ,Dash, R. N. ,Jalachandra Reddy, B. ,Venugopal, P. ,Sandeep, P. ,Madhavi, G. ,Raghavi, K. ,Sindhura, M. ,Prashanthi, R. ,Nalluri, B. N. ,Jain, P. S. ,Patel, M. K. ,Surana, S. J. ,Oniszczuk, A. ,Waksmundzka-Hajnos, M. ,Gadzikowska, M. ,Podgórski, R. ,Oniszczuk, T. ,Oniszczuk, T. , Influence of sample preparation methods on the quantitation of selected tropane alkaloids from herb of Datura innoxia mill. by HPTLC. Acta Chromatographica Vol. 25, no. 3/2013
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