Ion-pair RP-HPLC method for the estimation of eberconazole nitrate in bulk form and in pharmaceutical dosage forms

Czasopismo : Acta Chromatographica
Tytuł artykułu : Ion-pair RP-HPLC method for the estimation of eberconazole nitrate in bulk form and in pharmaceutical dosage forms

Autorzy :
Fatemi, M. H.
University of Mazandaran Laboratory of Chemometrics, Faculty of Chemistry Babolsar Iran, mhfatemi@umz.ac.ir,
Elyasi, M.
University of Mazandaran Laboratory of Chemometrics, Faculty of Chemistry Babolsar Iran,
Sowa, I.
Medical University of Lublin Department of Analytical Chemistry Chodźki 4a 20-093 Lublin Poland, i.sowa@umlub.pl,
Zajdel, D.
Medical University of Lublin Department of Analytical Chemistry Chodźki 4a 20-093 Lublin Poland,
Kocjan, R.
Medical University of Lublin Department of Analytical Chemistry Chodźki 4a 20-093 Lublin Poland,
Pizoń, M.
Medical University of Lublin Department of Analytical Chemistry Chodźki 4a 20-093 Lublin Poland,
Wójciak-Kosior, M.
Medical University of Lublin Department of Analytical Chemistry Chodźki 4a 20-093 Lublin Poland,
Hadad, G. M.
Suez Canal University Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Ismailia 41522 Egypt, ghhadad@hotmail.com,
Abdel-Salam, R. A.
Suez Canal University Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Ismailia 41522 Egypt, dr_rasalam@yahoo.com,
Abdel-Hameed, E. A.
Suez Canal University Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Ismailia 41522 Egypt,
Liu, W.-Y.
China Pharmaceutical University Department of Pharmaceutical Analysis 24 Tongjiaxiang Nanjing 210009 China, liuwenyuan8506@163.com,
Xie, S.-L.
China Pharmaceutical University Department of Pharmaceutical Analysis 24 Tongjiaxiang Nanjing 210009 China,
Xu, X. Z.
China Pharmaceutical University Department of Pharmaceutical Analysis 24 Tongjiaxiang Nanjing 210009 China,
Wu, C. Y.
China Pharmaceutical University Department of Pharmaceutical Analysis 24 Tongjiaxiang Nanjing 210009 China,
Feng, F.
China Pharmaceutical University Department of Phytochemistry 24 Tongjiaxiang Nanjing 210009 China,
Maślanka, A.
Jagiellonian University Medical College, Pharmaceutical Faculty, Department of Inorganic and Analytical Chemistry Medyczna 9 30-688 Krakow Poland,
Hubicka, U.
Jagiellonian University Medical College, Pharmaceutical Faculty, Department of Inorganic and Analytical Chemistry Medyczna 9 30-688 Krakow Poland,
Krzek, J.
Jagiellonian University Medical College, Pharmaceutical Faculty, Department of Inorganic and Analytical Chemistry Medyczna 9 30-688 Krakow Poland, jankrzek@cm-uj.krakow.pl,
Walczak, M.
Jagiellonian University Medical College, Pharmaceutical Faculty, Department of Pharmacokinetics and Physical Pharmacy Medyczna 9 30-688 Krakow Poland,
Izworski, G.
Jagiellonian University Medical College, Pharmaceutical Faculty, Department of Inorganic and Analytical Chemistry Medyczna 9 30-688 Krakow Poland,
Suchy, D.
Medical University of Silesia Department of Internal Medicine and Clinical Pharmacology Medyków 18 PL 40752 Katowice Poland, dariuszsuchy@gmail.com,
Łabuzek, K.
Medical University of Silesia Department of Internal Medicine and Clinical Pharmacology Medyków 18 PL 40752 Katowice Poland,
Pierzchała, O.
Medical University of Silesia Department of Internal Medicine and Clinical Pharmacology Medyków 18 PL 40752 Katowice Poland,
Okopień, B.
Medical University of Silesia Department of Internal Medicine and Clinical Pharmacology Medyków 18 PL 40752 Katowice Poland,
Vamsi Krishna, M.
Alliance Institute of Advanced Pharmaceutical and Health Sciences Ameerpet, Hyderabad 500038 Andhra Pradesh India, marothu_vamsi@rediffmail.com,
Dash, R. N.
Alliance Institute of Advanced Pharmaceutical and Health Sciences Ameerpet, Hyderabad 500038 Andhra Pradesh India,
Jalachandra Reddy, B.
Alliance Institute of Advanced Pharmaceutical and Health Sciences Ameerpet, Hyderabad 500038 Andhra Pradesh India,
Venugopal, P.
Alliance Institute of Advanced Pharmaceutical and Health Sciences Ameerpet, Hyderabad 500038 Andhra Pradesh India,
Sandeep, P.
Alliance Institute of Advanced Pharmaceutical and Health Sciences Ameerpet, Hyderabad 500038 Andhra Pradesh India,
Madhavi, G.
Alliance Institute of Advanced Pharmaceutical and Health Sciences Ameerpet, Hyderabad 500038 Andhra Pradesh India,
Abstrakty : An isocratic ion-pair reversed phase high-performance liquid chromatography-ultraviolet (RP-HPLC-UV) method for analysis of eberconazole nitrate in bulk and in pharmaceutical dosage forms has been developed and validated. Best separation was achieved on Lichrospher C18 column (250 mm × 4.6 mm, 5 μm) using a mobile phase of 10 mM potassium dihydrogen phosphate containing 10 mM tetra-butyl ammonium hydroxide (pH adjusted to 2.8 with ortho phosphoric acid) and methanol (75:25, v/v) at a flow rate of 1.0 mL min-1. UV detection was performed at 220 nm. The method was validated for specificity, linearity, precision, accuracy, limit of detection, limit of quantification, robustness, and solution stability. The calibration plot was linear over the concentration range of 10–80 μg mL-1(r2 = 0.999) and the limits of detection and quantification were 0.3 and 0.9 μg mL-1, respectively. Intra-day and inter-day precisions were 1.13% and 1.67%, respectively. Experimental design was employed to optimize the method. The method was successfully used for analysis of eberconazole nitrate in commercially available cream (Ebernet).

Słowa kluczowe : eberconazole nitrate, ion-pair RP-HPLC, method validation, pharmaceutical dosage forms,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2013
Numer : Vol. 25, no. 3
Strony : 503 – 518
Bibliografia : 1 S.C. Sweetman, Martindale: The Complete Drug Reference, 36th edn., Pharmaceutical Press, London, 2009, 530
2 M.J. Barbanoj, R. Antonijoan, C. Garcia-Gea, M. Puntes, I. Gich, and F. Jane, Methods Find. Exp. Clin. Pharmacol., 27, 227 (2005)
3 M. Torres-Rodríguez, R. Mendez, O.L. Jodra, Y. Morera, M. Espasa, T. Jimenez, and C. Lagunas, Antimicrob. Agents Chemother., 43, 1258 (1999)
4 R.J. Ekiert, J. Krzek, and P. Talik, Talanta, 82, 1090 (2010)
5 StatSoft, Inc., Statistica Data Analysis System, Statistica Software, Tulsa, OK, 2001
6 International Conference on Harmonization (ICH), Validation of Analytical Procedures: Text and Methodology Q2 (R1), Geneva, 2005
DOI :
Cytuj : Fatemi, M. H. ,Elyasi, M. ,Sowa, I. ,Zajdel, D. ,Kocjan, R. ,Pizoń, M. ,Wójciak-Kosior, M. ,Hadad, G. M. ,Abdel-Salam, R. A. ,Abdel-Hameed, E. A. ,Liu, W.-Y. ,Xie, S.-L. ,Xu, X. Z. ,Wu, C. Y. ,Feng, F. ,Maślanka, A. ,Hubicka, U. ,Krzek, J. ,Walczak, M. ,Izworski, G. ,Suchy, D. ,Łabuzek, K. ,Pierzchała, O. ,Okopień, B. ,Vamsi Krishna, M. ,Dash, R. N. ,Jalachandra Reddy, B. ,Venugopal, P. ,Sandeep, P. ,Madhavi, G. , Ion-pair RP-HPLC method for the estimation of eberconazole nitrate in bulk form and in pharmaceutical dosage forms. Acta Chromatographica Vol. 25, no. 3/2013
facebook