Isolation of gallic acid and methyl gallate from folium Toonea Sinensis and validated method for their quantitation using LC-based technologies

Czasopismo : Acta Chromatographica
Tytuł artykułu : Isolation of gallic acid and methyl gallate from folium Toonea Sinensis and validated method for their quantitation using LC-based technologies

Autorzy :
Patil, A. N.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India,
Nirmal, S. A.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India, nirmalsunil@rediffmail.com,
Chavan, A. K.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India,
Zhou, L.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Xu, J.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Luan, L.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Ma, J.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Gong, Y.
Ministry of Agriculture Institute for the Control of Agrochemicals Beijing 100026 China,
Qin, D.
Ministry of Agriculture Institute for the Control of Agrochemicals Beijing 100026 China,
Pan, C.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Jinlong, M.
Zhejiang University Department of Chemical and Biological Engineering Hangzhou 310027 China,
Guobin, J.
Dalian Nationalities University Environment and Resources College Dalian 11660 China,
Bo, J.
Dalian Nationalities University Life Science College Dalian 116600 China,
Hua, J.
Zhejiang University Department of Chemical and Biological Engineering Hangzhou 310027 China,
Montejo, C.
Universidad CEU San Pablo Departamento de Ciencias Farmacéuticas y de la Alimentación, Área de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28668 Madrid Spain, montejo@ceu.es,
Civera, C.
Universidad Complutense de Madrid Departamento de Química Física II, Facultad de Farmacia 28040 Madrid Spain,
Barcia, E.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Negro, S.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Fernández-Carballido, A.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Czauderna, M.
Polish Academy of Sciences The Kielanowski Institute of Animal Physiology and Nutrition 05-110 Jabłonna Poland, mr.czauderna@gmail.com,
Marounek, M.
Prague-Uhříněves The Institute of Animal Science Prague Czech Republic,
Duskova, D.
Prague-Uhříněves The Institute of Animal Science Prague Czech Republic,
Kowalczyk, J.
Polish Academy of Sciences The Kielanowski Institute of Animal Physiology and Nutrition 05-110 Jabłonna Poland,
Konda, R. K.
Hindu College of Pharmacy Department of Pharmaceutical Chemistry Amaravathi Road Guntur Andhrapradesh 522002 India, victory2ravi@yahoo.co.in,
Chandu, B. R.
Donbosco College of Pharmacy Department of Pharmaceutical Sciences Pulladigunta Guntur 522201 India,
Challa, B. R.
Nirmala College of Pharmacy Department of Pharmaceutical Analysis Madras Road Kadapa Andhrapradesh 516002 India, baluchalla_99@yahoo.com,
Chandrasekhar, K. B.
Jawaharlal Nehru Technological University Department of Chemistry Anantapur 515002 India,
Shen, Y.
Jiangsu University School of Pharmacy Zhenjiang China,
Yang, H.
Jiangsu University School of Pharmacy Zhenjiang China,
Xia, G.
Jiangsu University School of Pharmacy Zhenjiang China,
Wang, J.
Jiangsu University School of Pharmacy Zhenjiang China,
Cai, B.
Nanjing University of Chinese Medicine Jiangsu Key Laboratory of Chinese Medicine Processing, SATCM Key Laboratory of Standardization for Chinese Medicine Processing Nanjing China,
Jia, X.
Jiangsu University School of Pharmacy Zhenjiang China, jxiaobin2005@hotmail.com,
Abstrakty : Silica-gel column chromatography and preparative reversed-phase high-performance liquid chromatography (RP-HPLC) were sequentially employed for the isolation of two antioxidants including gallic acid and methyl gallate from Folium Toonea Sinensis. An RP-HPLC-UV method was then developed and validated to rapidly determine their content in this herb with ethyl gallate as internal standard. The quantitation was performed on an XBridge Shield RP18 column (150 mm × 4.6 mm, 5 μm) under 40°C. The mobile phase consisting of acetonitrile and 0.1% formic acid aqueous solution was driven at 1.0 mL min-1under gradient elution, and 270 nm was selected to monitor the separation. To evaluate the fitness for purpose of the method and to investigate the difference in the content of analytes among different samples, the leaves collected from five production sites were analyzed. The newly established method is suitable for routine analysis of gallic acid and methyl gallate in the herb and, hence, can assist in its quality assessment. It was also found that not only the content of two antioxidants but also the ratio varied significantly among different geographical origins. In addition, three samples from Yantai, Zumadian, and Zhenjiang were distinguished as they have a much higher content ratio than the other two.

Słowa kluczowe : Toona sinensis, Folium Toonea Sinensis, RP-HPLC-UV, gallic acid, methyl gallate,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2013
Numer : Vol. 25, no. 4
Strony : 687 – 701
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DOI :
Cytuj : Patil, A. N. ,Nirmal, S. A. ,Chavan, A. K. ,Zhou, L. ,Xu, J. ,Luan, L. ,Ma, J. ,Gong, Y. ,Qin, D. ,Pan, C. ,Jinlong, M. ,Guobin, J. ,Bo, J. ,Hua, J. ,Montejo, C. ,Civera, C. ,Barcia, E. ,Negro, S. ,Fernández-Carballido, A. ,Czauderna, M. ,Marounek, M. ,Duskova, D. ,Kowalczyk, J. ,Konda, R. K. ,Chandu, B. R. ,Challa, B. R. ,Chandrasekhar, K. B. ,Shen, Y. ,Yang, H. ,Xia, G. ,Wang, J. ,Cai, B. ,Jia, X. , Isolation of gallic acid and methyl gallate from folium Toonea Sinensis and validated method for their quantitation using LC-based technologies. Acta Chromatographica Vol. 25, no. 4/2013
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