Journal : Acta Chromatographica
Article : Level of hexabromocyclododecane isomers in the tissue of selected commonly consumed fish in Central European countries

Authors :
Kuc, J.
Cracow University of Technology Department of Chemical Engineering and Technology, Department of Analytical Chemistry Warszawska 24 31-155 Cracow Poland,,
Grochowalski, A.
Cracow University of Technology Department of Chemical Engineering and Technology, Department of Analytical Chemistry Warszawska 24 31-155 Cracow Poland,
Mach, S.
Institute of Public Health Ostrava Centre of Hygienic Laboratories Dobrá 240 739 51 Frýdek-Místek Czech Republic,
Placha, D.
VŠB-Technical University of Ostrava Centrum Nanotechnology 17 Listopadu 15/2172 708 33 Ostrava Czech Republic,
Abstract : This paper is focused on the determination of three hexabromocyclododecane isomers, α, β, and γ, in the tissue of the most commonly consumed marine and farmed fish in Central Europe. The analytical procedure contains multiple steps: extraction, dialysis using semipermeable membranes, and a clean-up step using a silica gel column. Hexabromocyclododecane (HBCD) isomers were determined by sensitive isotope dilution liquid chromatography-tandem mass spectrometry (LC-MS/MS) method which is characterized by a low limit of detection (1 pg g-1fresh weight). The recovery values obtained for individual isomers were in the range of 89–124%. The γ-HBCD isomer was detected in all samples. This isomer also had the largest percentage in comparison with the other isomers, except in salmon, in which the α-HBCD isomer was more abundant. The highest level of total HBCD was measured in mackerel (650 ± 195 pg g-1fresh weight). Cluster analysis allowed the differentiation of groups of objects and the display of objects based on the degree of integration within the same group. Spearman’s rank correlation indicated no statistically significant difference between the levels of HBCD and the fat content in the fish samples.

Keywords : fish, food contaminants, hexabromocyclododecane (HBCD), LC-MS,
Publishing house : University of Silesia in Katowice
Publication date : 2014
Number : Vol. 26, no. 4
Page : 575 – 585

: 1 M. Alaee, P. Arias, A. Sjödin, and A. Bergman, Environ. Int., 29, 683 (2003)
2 C.A. de Wit, Chemosphere, 46, 583 (2002)
3 EFSA The European Food Safety Authority, EFSA J., 328, 1 (2006)
4 N.V. Heeb, W.B. Schweizer, M. Kohler, and A.C. Gerecke, Chemosphere, 61, 65 (2005)
5 N.V. Heeb, W.B. Schweizer, P. Mattrel, R. Haag, and M. Kohler, Chemosphere, 66, 1590 (2007)
6 R.C. Thompson, Y. Olsen, R.P. Mitchell, A. Davis, S.J. Rowland, A.W. John, D. McGonigle, and A. Russell, Science, 304, 838 (2004)
7 R. Nakagawa, S. Murata, Y. Ashizuka, Y. Shintani, T. Hori, and T. Tsutsumi, Chemosphere, 81, 445 (2010)
8 S.D. Shaw, M.L. Berger, D. Brenner, K. Kannan, N. Lohmann, and O. Papke, Sci. Total Environ., 407, 3323 (2009)
9 G. Tomy, W. Budakowski, T. Halldorson, M. Whittle, M. Keir, and M. Alaee, Organohalogen Compd., 61, 111 (2003)
10 D. Ueno, M. Alaee, C. Marvin, D. Muir, G. Macinnis, E. Reiner, P. Crozier, V. Furdui, A. Subramanian, G. Fillmann, P.K. Lam, G.J. Zheng, M. Muchtar, H. Razak, M. Prudente, K. Chung, and S. Tanabe, Environ. Pollut., 144, 238 (2005)
11 M. Remberger, J. Sternbeck, A. Palm, L. Kaj, K. Strömberg, and E. Brorström- Lundén, Chemosphere, 54, 9 (2004)
12 NORDEN 2008. Hexabromocyclododecane as a possible global POP. TemaNord 2008:520. Nordic Council of Ministers, Copenhagen 2007. ISBN 978-92-893-1665-1
13 HBCD FactSheet Brominated Flame Retardant. Hexabromocyclododecane. BSEF. October 2012.
14 U. Sellström, A. Kierkegaard, C.A. de Wit, and B. Jansson, Environ. Toxicol. Chem., 17, 1065 (1998)
15 I. Covaci, A.C. Gerecke, R.J. Law, S. Voorspoels, M. Kohler, N.V. Heeb, H. Leslie, C.R. Allchin, and J. De Boer. Environ. Sci. Technol., 40, 3679 (2006)
16 C.A. de Wit, M. Alaee, and D.C.C. Muir, Chemosphere, 64, 209 (2006)
17 C.H. Marvin, G.T. Tomy, M. Alaee, and G. MacInnis, Chemosphere, 64, 268 (2006)
18 M. Kohler, M. Zennegg, C. Bogdal, A.C. Gerecke, P. Schmid, N.V. Heeb, M. Sturm, H. Vonmont, H.P. Kohler, and W. Giger, Environ. Sci. Technol., 42, 6378 (2008)
19 I. Thomsen, M. Froshaug, S.L. Broadwell, and G. Becher, Organohalogen Compd., 67, 509 (2005)
20 I. Thomsen, H. Stigum, M. Froshaug, S.L. Broadwell, G. Becher, and M. Eggesbo, Environ. Int., 36, 68 (2010)
21 I. Eljarrat, A. de la Cal, D. Raldua, C. Duran, and D. Barcelo, Environ. Sci. Technol., 38, 2603 (2004)
22 K. Janak, A. Covaci, S. Voorspoels, and G. Becher, Environ. Sci. Technol., 39, 1987 (2005)
23 R.J. Law, D. Herzke, S. Harrad, S. Morris, P. Bersuder, and C.R. Allchin, Chemosphere, 73, 223 (2008)
24 D.F.K. Rawn, A. Sadler, S.C. Quade, W.F. Sun, B.P Lau, I. Kosarac, S. Hayward, and J.J. Ryan, Food Addit. Contam. A., 28, 807–815 (2011)
25 I. Hu, X. Hu, J. Li, P. Wang, and S. Guo, J. Liq. Chromatogr., 35, 558 (2012)
26 R. Döring, J. Virtanen, L. Avdelas, E. Avdic, R. Beukers, R. Curtis, I. Davidjuka, M. Ebeling, A. Garret, J. Lees, C. McCarthy, L. Malvarosa, R. Mongruel, A. Motova, C. Moura, R. Nielsen, A. Paulrud, B. Pienkowska, H. Pokki, C. Stroie, and D. Vassallo, Report on the evaluation of data collected on the Fish Processing sector. Luxembourg 2011: Publications Office of the European Union, 217
27 B. Gómara, R. Lebron-Aguilar, J.E. Quintanilla-Lopez, and M.J. Gonzalez, Anal. Chim. Acta, 605, 53 (2007)
Qute : Kuc, J. ,Grochowalski, A. ,Mach, S. ,Placha, D. ,Placha, D. , Level of hexabromocyclododecane isomers in the tissue of selected commonly consumed fish in Central European countries. Acta Chromatographica Vol. 26, no. 4/2014