Method development and validation for simultaneous HPLC analysis of six active components of the Chinese medicine Qin-Bao-Hong antitussive tablet

Czasopismo : Acta Chromatographica
Tytuł artykułu : Method development and validation for simultaneous HPLC analysis of six active components of the Chinese medicine Qin-Bao-Hong antitussive tablet

Autorzy :
Dejaegher, B.
Vrije Universiteit Brussel — VUB Analytical Chemistry and Pharmaceutical Technology (FABI), Pharmaceutical Institute Laarbeeklaan 103 1090 Brussels Belgium,
Vander Heyden, Y.
Vrije Universiteit Brussel — VUB Analytical Chemistry and Pharmaceutical Technology (FABI), Pharmaceutical Institute Laarbeeklaan 103 1090 Brussels Belgium, yvanvdh@vub.ac.be,
Durón, R. R.
Universidad Autónoma de Nuevo León Departamento de Química Analítica, Facultad de Medicina P.O. Box 2316 Sucursal Tecnológico 64841 Monterrey Nuevo León México,
Almaguer, L. C.
Universidad Autónoma de Nuevo León Departamento de Química Analítica, Facultad de Medicina P.O. Box 2316 Sucursal Tecnológico 64841 Monterrey Nuevo León México,
Garza-Juárez, A.
Universidad Autónoma de Nuevo León Departamento de Química Analítica, Facultad de Medicina P.O. Box 2316 Sucursal Tecnológico 64841 Monterrey Nuevo León México,
Salazar Cavazos, M. L.
Universidad Autónoma de Nuevo León Departamento de Química Analítica, Facultad de Medicina P.O. Box 2316 Sucursal Tecnológico 64841 Monterrey Nuevo León México,
Waksman de Torres, N.
Universidad Autónoma de Nuevo León Departamento de Química Analítica, Facultad de Medicina P.O. Box 2316 Sucursal Tecnológico 64841 Monterrey Nuevo León México, nwaksman@fm.uanl.mx,
Garza-Juárez, A.
UANL, México Department of Analytical Chemistry, Facultad de Medicina Maderoy Aguirre Pequeño Col. Mitras Centro C.P. 64460 Mty, N.L. México,
Waksman de Torres, N.
UANL, México Department of Analytical Chemistry, Facultad de Medicina Maderoy Aguirre Pequeño Col. Mitras Centro C.P. 64460 Mty, N.L. México,
Salazar Cavazos, M. L.
UANL, México Department of Analytical Chemistry, Facultad de Medicina Maderoy Aguirre Pequeño Col. Mitras Centro C.P. 64460 Mty, N.L. México, masalaz52@yahoo.com,
Durón, R. R.
UANL, México Department of Analytical Chemistry, Facultad de Medicina Maderoy Aguirre Pequeño Col. Mitras Centro C.P. 64460 Mty, N.L. México,
Briciu, R. D.
Babeş-Bolyai University Faculty of Chemistry and Chemical Engineering Arany Janos Str. No 11 RO-400028 Cluj Napoca Romania,
Kot-Wasik, A.
Gdańsk University of Technology Chemical Faculty Narutowicza Str. No 11/12 80-952 Gdańsk Poland,
Namieśnik, J.
Gdańsk University of Technology Chemical Faculty Narutowicza Str. No 11/12 80-952 Gdańsk Poland,
Sârbu, C.
Babeş-Bolyai University Faculty of Chemistry and Chemical Engineering Arany Janos Str. No 11 RO-400028 Cluj Napoca Romania, costelsrb@yahoo.co.uk,
Sławik, T.
Medical University of Lublin Department of Medicinal Chemistry, Pharmaceutical Faculty Jaczewskiego 4 20-090 Lublin Poland,
Skibiński, R.
Medical University of Lublin Department of Medicinal Chemistry, Pharmaceutical Faculty Jaczewskiego 4 20-090 Lublin Poland, robert.skibinski@am.lublin.pl,
Paw, B.
Medical University of Lublin Department of Medicinal Chemistry, Pharmaceutical Faculty Jaczewskiego 4 20-090 Lublin Poland,
Działo, G.
Medical University of Lublin Department of Medicinal Chemistry, Pharmaceutical Faculty Jaczewskiego 4 20-090 Lublin Poland,
Sajewicz, M.
Silesian University Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Matlengiewicz, M.
Silesian University Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Kronenbach, D.
Silesian University Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Gontarska, M.
Silesian University Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Kowalska, T.
Silesian University Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland, kowalska@us.edu.pl,
Ekiert, R. J.
Jagiellonian University Department of Inorganic and Analytical Chemistry, Collegium Medicum 9 Medyczna St 30-688 Kraków Poland,
Krzek, J.
Jagiellonian University Department of Inorganic and Analytical Chemistry, Collegium Medicum 9 Medyczna St 30-688 Kraków Poland, jankrzek@cm-uj.krakow.pl,
Czekaj, J. S.
Jagiellonian University Department of Inorganic and Analytical Chemistry, Collegium Medicum 9 Medyczna St 30-688 Kraków Poland,
Hubicka, U.
Jagiellonian University Department of Inorganic and Analytical Chemistry, Collegium Medicum 9 Medyczna St 30-688 Kraków Poland,
Azeem, A.
Faculty of Pharmacy Department of Pharmaceutics Jamia Hamdard, New Delhi 110062 India,
Rizwan, M.
Faculty of Pharmacy Department of Pharmaceutics Jamia Hamdard, New Delhi 110062 India,
Ahmad, F. J.
Faculty of Pharmacy Department of Pharmaceutics Jamia Hamdard, New Delhi 110062 India,
Iqbal, Z.
Faculty of Pharmacy Department of Pharmaceutics Jamia Hamdard, New Delhi 110062 India,
Khar, R. K.
Faculty of Pharmacy Department of Pharmaceutics Jamia Hamdard, New Delhi 110062 India,
Aqil, M.
Faculty of Pharmacy Department of Pharmaceutics Jamia Hamdard, New Delhi 110062 India,
Talegaonkar, S.
Faculty of Pharmacy Department of Pharmaceutics Jamia Hamdard, New Delhi 110062 India, stalegaonkar@jamiahamdard.ac.in,
Bhusari, V. K.
Bharati Vidyapeeth University, Poona College of Pharmacy Department of Pharmaceutical Chemistry Pune Maharashtra India 411038,
Mahadik, M. V.
Bharati Vidyapeeth University, Poona College of Pharmacy Department of Pharmaceutical Chemistry Pune Maharashtra India 411038,
Dhaneshwar, S. R.
Bharati Vidyapeeth University, Poona College of Pharmacy Department of Pharmaceutical Chemistry Pune Maharashtra India 411038, sunil.dhaneshwar@gmail.com,
Patel, H. U.
Shri Sarvajanik Pharmacy College Department of Pharmaceutical Chemistry Near Arvind Baug Mehsana 384001 Gujarat India,
Suhagia, B. N.
L.M. College of Pharmacy Department of Pharmaceutical Chemistry Navrangpura, Ahmedabad 380009 Gujarat India,
Patel, C. N.
Shri Sarvajanik Pharmacy College Department of Pharmaceutical Chemistry Near Arvind Baug Mehsana 384001 Gujarat India, harshaupatel@yahoo.co.in,
Liu, X. X.
China Pharmaceutical University Department of Chinese Materia Medica Analysis Nanjing 210038 China,
Wang, L.
China Pharmaceutical University Department of Phytochemistry Nanjing 210038 China,
Yang, J.
China Pharmaceutical University Department of Chinese Materia Medica Analysis Nanjing 210038 China,
Zhang, T. T.
China Pharmaceutical University Department of Chinese Materia Medica Analysis Nanjing 210038 China,
Deng, X. D.
China Pharmaceutical University Department of Chinese Materia Medica Analysis Nanjing 210038 China,
Wang, Q.
China Pharmaceutical University Department of Chinese Materia Medica Analysis Nanjing 210038 China, qwang49@126.com,
Chen, J.
Chinese Academy of Sciences Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P.R. China,
Wang, G. Y.
Chinese Academy of Sciences Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P.R. China,
Shi, Y. P.
Chinese Academy of Sciences Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P.R. China, shiyp@lzb.ac.cn,
Abstrakty : A high-performance liquid chromatographic (HPLC) method coupled with photodiode array (PDA) detection has been developed and validated for simultaneous analysis of six active components (syringin, hyperoside, baicalin, quercetin, baicalein, and farrerol) of the Chinese medicinal preparation Qin-Bao-Hong antitussive tablet. The optimum conditions for separation were achieved on a 3.9 mm × 150 mm i.d., 5-μm particle, C 18 column with a linear mobile phase gradient prepared from acetonitrile and 1% acetic acid at a flow rate of 1.0 mL min-1. Because of the different UV characteristics of these compounds, four detection wavelengths were used for the quantitative analysis (265 nm for syringin, 256 nm for hyperoside and quercetin, 277 nm for baicalin and baicalein, and 296 nm for farrerol). For all the analytes a good linear regression relationship ( r > 0.999) was obtained between peak area and concentration over a relatively wide range. The method was validated for repeatability, precision, stability, accuracy, selectivity, and robustness. The validated method was successfully applied to simultaneous analysis of these active components in Qin-Bao-Hong antitussive tablet from different production batches.

Słowa kluczowe : high-performance liquid chromatography, traditional chinese medicine, Qin-Bao-Hong antitussive tablet, quality control,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2009
Numer : Vol. 21, no. 2
Strony : 341 – 354
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DOI :
Cytuj : Dejaegher, B. ,Vander Heyden, Y. ,Durón, R. R. ,Almaguer, L. C. ,Garza-Juárez, A. ,Salazar Cavazos, M. L. ,Waksman de Torres, N. ,Garza-Juárez, A. ,Waksman de Torres, N. ,Salazar Cavazos, M. L. ,Durón, R. R. ,Briciu, R. D. ,Kot-Wasik, A. ,Namieśnik, J. ,Sârbu, C. ,Sławik, T. ,Skibiński, R. ,Paw, B. ,Działo, G. ,Sajewicz, M. ,Matlengiewicz, M. ,Kronenbach, D. ,Gontarska, M. ,Kowalska, T. ,Ekiert, R. J. ,Krzek, J. ,Czekaj, J. S. ,Hubicka, U. ,Azeem, A. ,Rizwan, M. ,Ahmad, F. J. ,Iqbal, Z. ,Khar, R. K. ,Aqil, M. ,Talegaonkar, S. ,Bhusari, V. K. ,Mahadik, M. V. ,Dhaneshwar, S. R. ,Patel, H. U. ,Suhagia, B. N. ,Patel, C. N. ,Liu, X. X. ,Wang, L. ,Yang, J. ,Zhang, T. T. ,Deng, X. D. ,Wang, Q. ,Chen, J. ,Wang, G. Y. ,Shi, Y. P. , Method development and validation for simultaneous HPLC analysis of six active components of the Chinese medicine Qin-Bao-Hong antitussive tablet. Acta Chromatographica Vol. 21, no. 2/2009
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