Optimum separation condition of five bioactive compounds by HCI program in HPLC

Czasopismo : Acta Chromatographica
Tytuł artykułu : Optimum separation condition of five bioactive compounds by HCI program in HPLC

Autorzy :
Sajewicz, M.
University of Silesia Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Gontarska, M.
University of Silesia Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Kronenbach, D.
University of Silesia Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Berry, E.
University of Silesia Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Kowalska, T.
University of Silesia Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland, teresa.kowalska@us.edu.pl,
Deshpande, P. B.
A.I.S.S.M.S. College of Pharmacy Department of Pharmaceutical Analysis Kennedy Road Pune 411001 MH India, padmanabh77@yahoo.co.in,
Gandhi, S. V.
A.I.S.S.M.S. College of Pharmacy Department of Pharmaceutical Analysis Kennedy Road Pune 411001 MH India,
Gaikwad, N. V.
A.I.S.S.M.S. College of Pharmacy Department of Pharmaceutical Analysis Kennedy Road Pune 411001 MH India,
Khandagle, K. S.
A.I.S.S.M.S. College of Pharmacy Department of Pharmaceutical Analysis Kennedy Road Pune 411001 MH India,
Sateesh, J. N.
PRRM College of Pharmacy Department of Chemistry Kadapa Andhra Pradesh India, nagasateesh@gmail.com,
Subba Reedy, G. V.
JNTUA College of Engineering Department of Chemistry Pulivendula, Y.S.R (Kadapa) Andhra Pradesh India,
Jayaveera, K. N.
awaharlal Nehru Technological University Anantapur Oil Technology Research Institute Anantapur Andhra Pradesh India,
Dhayalamurthi, S.
Mother Theresa Post Graduate & Research Institute of Health Sciences Department of Chemistry, College of Pharmacy Gorimedu Puducherry India,
Anandakumar, K.
Adhiparasakthi College of Pharmacy Department of Pharmaceutical Analysis Melmaruvathur 603 319 Tamil Nadu India, anandkarunakaran@gmail.com,
Jothieswari, D.
Adhiparasakthi College of Pharmacy Department of Pharmaceutical Analysis Melmaruvathur 603 319 Tamil Nadu India,
Subathrai, R.
Adhiparasakthi College of Pharmacy Department of Pharmaceutical Analysis Melmaruvathur 603 319 Tamil Nadu India,
Santhi, D. V.
Adhiparasakthi College of Pharmacy Department of Pharmaceutical Analysis Melmaruvathur 603 319 Tamil Nadu India,
Vetrichelvan, T.
Adhiparasakthi College of Pharmacy Department of Pharmaceutical Analysis Melmaruvathur 603 319 Tamil Nadu India,
Shah, D. A.
Indukaka Ipcowala College of Pharmacy P.B. No. 53 New Vallabh Vidyanagar 388 121 Dist. Anand Gujarat India, dimalgroup@yahoo.com,
Bhatt, K. K.
Indukaka Ipcowala College of Pharmacy P.B. No. 53 New Vallabh Vidyanagar 388 121 Dist. Anand Gujarat India,
Baldania, S. L.
Indukaka Ipcowala College of Pharmacy P.B. No. 53 New Vallabh Vidyanagar 388 121 Dist. Anand Gujarat India,
Tian, M.
Inha University Department of Chemical Engineering 253 Yonghyun-Dong, Nam-Ku Incheon 402-751 Korea,
Li, S.
Inha University Department of Chemical Engineering 253 Yonghyun-Dong, Nam-Ku Incheon 402-751 Korea,
Row, K. H.
Inha University Department of Chemical Engineering 253 Yonghyun-Dong, Nam-Ku Incheon 402-751 Korea, rowkho@inha.ac.kr,
Abstrakty : An efficient method used to separate five bioactive compounds from Gelidium amansii was optimized by the HCI software. The optimum composition of mobile phase for high-performance liquid chromatographic (HPLC) separation was obtained. The elution profiles were calculated by the polynomial theory based on the retention factor ln k = A + BF + CF2 (F was the volume percentage of acetonitrile with 1.0% acetic acid); then, the theory was applied to calculate the elution profile in both isocratic and gradient modes by modifying different mobile phase conditions with HCI program. The calculated results of mobile phase condition suggested that acetonitrile-water (containing 1.0% acetic acid) with a linear gradient elution of 0∼30 min from 15:85 to 50:50 (v/v) was the optimized component. In the experimental conditions, the agreement between the experimental elution profiles and the calculated values of eluted concentration was relatively good.

Słowa kluczowe : HCI program, HPLC, separation, bioactive compounds, marine plant,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2012
Numer : Vol. 24, no. 1
Strony : 65 – 74
Bibliografia : 1 K.H.M. Cardozo, T. Guaratini, M.P. Barros, V.R. Falcao, A.P. Tonon, N.P. Lopes, S. Campos, M.A. Torres, A.O. Souza, P. Colepicolo, and E. Pinto, Comp. Biochem. Physiol. C: Toxicol. Pharmacol., 146, 60 (2007)
2 N.T. Eriksen, Appl. Microbiol. Biotech., 80, 1 (2008)
3 M. Plaza, A. Cifuentes, and E. Ibanez, Trends Food Sci. Technol., 19, 31 (2008)
4 M. Gantar and Z. Svircev, J. Phycol., 44(2), 260 (2008)
5 F. Bettazzi, I. Palchetti, S. Sisalli, and M. Mascini, Anal. Chim. Acta, 555(1), 134 (2006)
6 H.B. Li, K.W. Cheng, C.C. Wong, K.W. Fan, F. Chen, and Y. Jiang, Food Chem., 102, 771 (2007)
7 P. Ganesan, C.S. Kumar, and N. Bhaskar, Bioresour. Technol., 99, 2717 (2008)
8 A.K. Sinha, S.C. Verma, and U.K. Sharma, J. Sep. Sci., 30, 15 (2007)
9 E. Cavoy, M.F. Deltent, S. Lehoucq, and D. Miggiano, J. Chromatogr. A, 769, 49 (1997)
10 J.H. Kennedy, M.D. Belvo, V.S. Sharp, and J.D. Williams, J. Chromatogr. A, 1046, 55 (2004)
11 X.J. Yao, X.Y. Zhang, R.S. Zhang, M.C. Liu, Z.D. Hu, and B.T. Fan, Talanta, 57, 297 (2002)
12 L. Jin, D.Y. Choi, Y. Wang, W.S. Ahn, D.K. Choi, and K.H. Row, J. Ind. Eng. Chem., 11(4), 615 (2005)
13 D.H. Kang, J.W. Lee, and K.H. Row, J. Liq. Chrom. Relat. Technol., 24(6), 855 (2001)
14 C.H. Jin, J.W. Lee, and K.H. Row, J. Sep. Sci., 31, 23 (2008)
15 R. Kaliszan, P. Wiczling, and M.J. Markuszewski, J. Chromatogr. A, 1060, 165 (2004)
16 J. Zheng and K.H. Row, Chin. J. Chem. Eng., 15(2), 291 (2007)
17 Y. Jin and K.H. Row, Chin. J. Chromatogr., 24(5), 466 (2006)
18 S.V. Galushko, A.A. Kamenchuk, and G.L. Pit, J. Chromatogr. A, 660, 47 (1994)
19 L.R. Snyder and J.W. Dolan, Adv. Chromatogr., 38, 115 (1998)
20 L.R. Snyder, J.W. Dolan, and J.R. Gant, J. Chromatogr., 165, 3 (1979)
21 W. Markowski and W. Golkiewicz, Chromatographia, 25, 339 (1988)
22 P.J. Schoenmakers, H.A.H. Billiet, and L. Galan, J. Chromatogr. A, 185, 179 (1979)
DOI :
Cytuj : Sajewicz, M. ,Gontarska, M. ,Kronenbach, D. ,Berry, E. ,Kowalska, T. ,Deshpande, P. B. ,Gandhi, S. V. ,Gaikwad, N. V. ,Khandagle, K. S. ,Sateesh, J. N. ,Subba Reedy, G. V. ,Jayaveera, K. N. ,Dhayalamurthi, S. ,Anandakumar, K. ,Jothieswari, D. ,Subathrai, R. ,Santhi, D. V. ,Vetrichelvan, T. ,Shah, D. A. ,Bhatt, K. K. ,Baldania, S. L. ,Tian, M. ,Li, S. ,Row, K. H. , Optimum separation condition of five bioactive compounds by HCI program in HPLC. Acta Chromatographica Vol. 24, no. 1/2012
facebook