Journal : Acta Chromatographica
Article : Quality evaluation of Yanghuo Sanqi tablet through a simultaneous determination of five major active flavonoids and three main saponins by HPLC-DAD-ELSD

Authors :
Petruczynik, A.
Medical University of Lublin Department of Inorganic Chemistry 4a Chodźki Str. 20-093 Lublin Poland, annapetruczynik@poczta.onet.pl,
Waksmundzka-Hajnos, M.
Medical University of Lublin Department of Inorganic Chemistry 4a Chodźki Str. 20-093 Lublin Poland,
Rykowska, I.
Adam Mickiewicz University Faculty of Chemistry Umultowska 89b 61-614 Poznań Poland, obstiwo@amu.edu.pl,
Wasiak, W.
Adam Mickiewicz University Faculty of Chemistry Umultowska 89b 61-614 Poznań Poland,
Inglot, T. W.
Medical University of Lublin Department of Medicinal Chemistry, Faculty of Pharmacy Jaczewskiego 4 20-090 Lublin Poland,
Gumieniczek, A.
anna.gumieniczek@am.lublin.pl,
Komsta, Ł.
Medical University of Lublin Department of Medicinal Chemistry, Faculty of Pharmacy Jaczewskiego 4 20-090 Lublin Poland,
Związek, R.
Medical University of Lublin Department of Medicinal Chemistry, Faculty of Pharmacy Jaczewskiego 4 20-090 Lublin Poland,
Sharaf El Din, M. K.
Mansoura University Department of Analytical Chemistry, Faculty of Pharmacy Mansoura 35516 Egypt,
Ibrahim, F.
Mansoura University Department of Analytical Chemistry, Faculty of Pharmacy Mansoura 35516 Egypt,
Eid, M. I.
Mansoura University Department of Analytical Chemistry, Faculty of Pharmacy Mansoura 35516 Egypt,
Wahba, M. E. K.
Mansoura University Department of Analytical Chemistry, Faculty of Pharmacy Mansoura 35516 Egypt, marywahba2004@yahoo.com,
El-Shaboury, S. R.
Assiut University Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Assiut 71526 Egypt,
Hussein, A. S.
Assiut University Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Assiut 71526 Egypt,
Mohamed, N. A.
Assiut University Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Assiut 71526 Egypt, mohamed_mounir2000@yahoo.com,
El-Sutohy, M. M.
Al-Azhar University, Assiut Branch Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Assiut 71524 Egypt,
Madej, K.
Jagiellonian University Department of Analytical Chemistry, Faculty of Chemistry Ingardena St. 3 30-060 Krakow Poland, madejk@chemia.uj.edu.pl,
Persona, K.
Jagiellonian University Department of Analytical Chemistry, Faculty of Chemistry Ingardena St. 3 30-060 Krakow Poland,
Nizio, M.
Jagiellonian University Department of Analytical Chemistry, Faculty of Chemistry Ingardena St. 3 30-060 Krakow Poland,
Emami, J.
Isfahan University of Medical Sciences Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences Isfahan Iran, emami@pharm.mui.ac.ir,
Rezazadeh, M.
Isfahan University of Medical Sciences Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences Isfahan Iran,
Smolarz, H. D.
Medical University of Lublin Chair and Department of Pharmaceutical Botany Lublin Poland, h.smolarz@am.lublin.pl,
Hałka, A.
Medical University of Lublin Department of Physical Chemistry, Chair of Chemistry Lublin Poland,
Chabros, O.
Medical University of Lublin Chair and Department of Pharmaceutical Botany Lublin Poland,
Dzido, T. H.
Medical University of Lublin Department of Physical Chemistry, Chair of Chemistry Lublin Poland,
Rode, S.
Saraswati Vidya Bhawan’s College of Pharmacy Sonarpada, Dombivli (East) 421 204 Maharashtra India,
Bhujbal, P.
Saraswati Vidya Bhawan’s College of Pharmacy Sonarpada, Dombivli (East) 421 204 Maharashtra India,
Sandhya, P.
Saraswati Vidya Bhawan’s College of Pharmacy Sonarpada, Dombivli (East) 421 204 Maharashtra India, iamsandhyap@yahoo.com,
Zhu, F. X.
Jiangsu Provincial Academy of Chinese Medicine Key Laboratory of New Drug Delivery System of Chinese Meteria Medica No. 100 Shizi Road Nanjing 210028 China,
Jia, X. B.
Jiangsu Provincial Academy of Chinese Medicine Key Laboratory of New Drug Delivery System of Chinese Meteria Medica No. 100 Shizi Road Nanjing 210028 China,
Cai, Y.
Jiangsu Provincial Academy of Chinese Medicine Key Laboratory of New Drug Delivery System of Chinese Meteria Medica No. 100 Shizi Road Nanjing 210028 China,
Chen, Y.
Jiangsu Provincial Academy of Chinese Medicine Key Laboratory of New Drug Delivery System of Chinese Meteria Medica No. 100 Shizi Road Nanjing 210028 China,
Abstract : Yanghuo Sanqi tablet (YST), combined prescription mainly derived from the leaves of herba epimedii and the roots of Panax notoginseng, is a traditional Chinese medicine (TCM). Flavonoids (icarrin, epimedin A, epimedin B, epimedin C, and baohuoside I) and saponins (notoginsenoside R1, ginsenoside Rgl, and ginsenoside Rbl) are considered as the main bioactive compounds of YST. However, there is no report on quality control of TCMs by simultaneous determination of above-mentioned flavonoids and saponins so far. In this work, for the first time, a high-performance liquid chromatography-diode array detector-evaporative light-scattering detector (HPLC-DAD-ELSD) method was developed to evaluate the quality of YST through a simultaneous determination of five major active flavonoids and three main saponins. Optimum separations were obtained with a Zorbax SB-C18 column by gradient elution with acetonitrile-water as the mobile phase. The drift tube temperature of ELSD was set at 105 °C, and the nebulizing gas flow rate was 2.5 L min-1. The fully validated method was successfully applied to quantify the eight bioactive components in three lot products. This simple, low-cost, and reliable HPLC-DAD-ELSD method provided a new basis for assessing the quality of traditional Chinese medicinal compound preparations (TCMCPs) consisting of many bioactive components.

Keywords : Yanghuo Sanqi, quality evaluation, flavonoid, saponin, icarrin, ginsenoside, HPLC-DAD-ELSD,
Publishing house : University of Silesia in Katowice
Publication date : 2013
Number : Vol. 25, no. 1
Page : 147 – 157

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DOI :
Qute : Petruczynik, A. ,Waksmundzka-Hajnos, M. ,Rykowska, I. ,Wasiak, W. ,Inglot, T. W. ,Gumieniczek, A. ,Komsta, Ł. ,Związek, R. ,Sharaf El Din, M. K. ,Ibrahim, F. ,Eid, M. I. ,Wahba, M. E. K. ,El-Shaboury, S. R. ,Hussein, A. S. ,Mohamed, N. A. ,El-Sutohy, M. M. ,Madej, K. ,Persona, K. ,Nizio, M. ,Emami, J. ,Rezazadeh, M. ,Smolarz, H. D. ,Hałka, A. ,Chabros, O. ,Dzido, T. H. ,Rode, S. ,Bhujbal, P. ,Sandhya, P. ,Zhu, F. X. ,Jia, X. B. ,Cai, Y. ,Chen, Y. ,Chen, Y. , Quality evaluation of Yanghuo Sanqi tablet through a simultaneous determination of five major active flavonoids and three main saponins by HPLC-DAD-ELSD. Acta Chromatographica Vol. 25, no. 1/2013
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