Quantitative HPLC analysis of flavonoids and chlorogenic acid in the leaves and inflorescences of Prunus serotina Ehrh

Czasopismo : Acta Chromatographica
Tytuł artykułu : Quantitative HPLC analysis of flavonoids and chlorogenic acid in the leaves and inflorescences of Prunus serotina Ehrh

Autorzy :
Sherma, J.
Department of Chemistry, Lafayette College, Easton, PA 18042, USA,
Vrchotová, N.
Institute of Systems Biology and Ecology AS CR, Branišovská 31, 370 05 České Budějovice, Czech Republic,
Sivakumar, T.
Department of Pharmacy, Faculty of Engineering and Technology, Annamalai University, Annamalainagar, Tamil Nadu-608 002, India,
Petruczynik, A.
Department of Inorganic Chemistry, Medical University, Staszica 6, 20-081 Lublin, Poland,
Benkő, B.
PhD School of Semmelweis University, 1085 Budapest, Üllői út 26, Hungary, Division of Pharmacology and Drug Safety, Richter Gedeon Rt., Gyömrői út 21, 1475 Budapest, P.O. Box 27, Hungary, Department of Chemistry and Biochemistry, University of Pécs, Péc,
Mori, M.
Department of Chemistry, Faculty of Engineering, Gunma University, 1-5-1 Tenjin-cho, Kiryu, 376-8515, Japan,
Solangi, A. R.
National Center of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, Pakistan,
Idris, A. M.
Department of Chemistry, College of Science, King Faisal University, Hofuf 31982, Saudi Arabia,
Milán-Segovia, R.
Laboratorio de Farmacia, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Ave. Manuel Nava No. 6, 78290 San Luis Potosí, S.L.P., Mexico,
Qutab, S. S.
Schazoo Laboratories (Pvt) Ltd, 45 G.T. Road, Lahore-54000, Pakistan,
Agil, M.
Faculty of Pharmacy, Hamdard University New Delhi, 110 062, India,
Dhaneshwar, S. S.
Department of Pharmaceutical Chemistry, Poona College of Pharmacy, Bharati Vidyapeeth University, Erandwane, Pune-411 038, India,
Gannu, R.
Centre for Biopharmaceutics and Pharmacokinetics, University College of Pharmaceutical Sciences, Kakatiya University, Warangal, Andhra Pradesh, India,
Popović, G.
Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, P.O. Box 146, 11000 Belgrade, Serbia,
Mhaske, D. V.
Department of Quality Assurance Techniques and Pharmaceutical Chemistry, Bharati Vidyapeeth University, Poona College of Pharmacy, Centre for Advanced Pharmaceutical Research, Erandwane, Pune 411038, Maharashtra State, India,
Mohammad, A.
Analytical Research Laboratory, Department of Applied Chemistry, Faculty of Engineering and Technology, Aligarh Muslim University, Aligarh 202002, India,
Jain, G. K.
Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, New Delhi, 110062, India,
Dembitsky, V. M.
Department of Medicinal Chemistry and Natural Products, School of Pharmacy, P.O. Box 12065, The Hebrew University of Jerusalem, Jerusalem 91120, Israel,
Qiao, C. F.
Chinese Medicine Laboratory, Hong Kong Jockey Club Institute of Chinese Medicine, Hong Kong, China,
Waksmundzka-Hajnos, M.
Department of Inorganic Chemistry, Medical University of Lublin, Staszica 6, 20-081 Lublin, Poland,
Codreanu, M. V.
Department of Pharmaceutical Botany, “Carol Davila” University of Medicine and Pharmacy, 6 Traian Vuia Street, Bucharest, 70139, Romania,
Singh, D. P.
Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute, Lucknow, India,
Olszewska, M.
Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Łódź, 1 Muszyński Street, 90-151 Łódź, Poland,
Abstrakty : Two quantitative reversed-phase high-performance liquid chromatographic (RP-HPLC) methods with UV detection have been developed for the separation and quantification of the flavonoids in Prunus serotina Ehrh. leaves and inflorescences. The first method entails hydrolysis of the flavonoid compounds and subsequent chromatographic assay of the aglycones obtained, quercetin and kaempferol, for rapid and simple quantification of the total flavonoid content (125 mm × 4 mm, 5-µm particle, ODS column; mobile phase 40–60% methanol gradient in a 0.5% aqueous solution of orthophosphoric acid; run time 7 min). The second method is a direct procedure for simultaneous quantification of six glycosidic compounds (rutin, hyperoside, reynoutrin, guajiverin, avicularin, and juglanin) and chlorogenic acid (250 mm × 4 mm, 5-µm particle, LiChrospher 100 column; mobile phase acetonitrile gradient in a 0.5% aqueous solution of orthophosphoric acid). The precision, accuracy, and reproducibility of the methods used were apparent from good linearity (R = 0.9805–0.9995), recovery of the internal standards (93.5–98.5%), and low RSD values (0.4–7.2% for biological plant material). The method for analysis of the aglycones, and its simple modifications can, moreover, be recommended for qualitative and quantitative analysis of three common flavonols – quercetin, kaempferol, and isorhamnetin. This was shown for hydrolysed extracts obtained from six popular medicinal plants (Hypericum perforatum, Sambucus nigra, Calen-dula officinalis, Solidago virgaurea, Tilia sp., and Gingko biloba).

Słowa kluczowe :
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2007
Numer : No. 19
Strony : 253 – 269
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DOI :
Cytuj : Sherma, J. ,Vrchotová, N. ,Sivakumar, T. ,Petruczynik, A. ,Benkő, B. ,Mori, M. ,Solangi, A. R. ,Idris, A. M. ,Milán-Segovia, R. ,Qutab, S. S. ,Agil, M. ,Dhaneshwar, S. S. ,Gannu, R. ,Popović, G. ,Mhaske, D. V. ,Mohammad, A. ,Jain, G. K. ,Dembitsky, V. M. ,Qiao, C. F. ,Waksmundzka-Hajnos, M. ,Codreanu, M. V. ,Singh, D. P. ,Olszewska, M. , Quantitative HPLC analysis of flavonoids and chlorogenic acid in the leaves and inflorescences of Prunus serotina Ehrh. Acta Chromatographica No. 19/2007
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