Journal : Acta Chromatographica
Article : Quantitative analysis of marker constituent swertisin in Enicostemma hyssopifolium verdoon by RP-HPLC and HPTLC

Authors :
Sajewicz, M.
University of Silesia Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Gontarska, M.
University of Silesia Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Kronenbach, D.
University of Silesia Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Berry, E.
University of Silesia Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Kowalska, T.
University of Silesia Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland, teresa.kowalska@us.edu.pl,
Deshpande, P. B.
A.I.S.S.M.S. College of Pharmacy Department of Pharmaceutical Analysis Kennedy Road Pune 411001 MH India, padmanabh77@yahoo.co.in,
Gandhi, S. V.
A.I.S.S.M.S. College of Pharmacy Department of Pharmaceutical Analysis Kennedy Road Pune 411001 MH India,
Gaikwad, N. V.
A.I.S.S.M.S. College of Pharmacy Department of Pharmaceutical Analysis Kennedy Road Pune 411001 MH India,
Khandagle, K. S.
A.I.S.S.M.S. College of Pharmacy Department of Pharmaceutical Analysis Kennedy Road Pune 411001 MH India,
Sateesh, J. N.
PRRM College of Pharmacy Department of Chemistry Kadapa Andhra Pradesh India, nagasateesh@gmail.com,
Subba Reedy, G. V.
JNTUA College of Engineering Department of Chemistry Pulivendula, Y.S.R (Kadapa) Andhra Pradesh India,
Jayaveera, K. N.
awaharlal Nehru Technological University Anantapur Oil Technology Research Institute Anantapur Andhra Pradesh India,
Dhayalamurthi, S.
Mother Theresa Post Graduate & Research Institute of Health Sciences Department of Chemistry, College of Pharmacy Gorimedu Puducherry India,
Anandakumar, K.
Adhiparasakthi College of Pharmacy Department of Pharmaceutical Analysis Melmaruvathur 603 319 Tamil Nadu India, anandkarunakaran@gmail.com,
Jothieswari, D.
Adhiparasakthi College of Pharmacy Department of Pharmaceutical Analysis Melmaruvathur 603 319 Tamil Nadu India,
Subathrai, R.
Adhiparasakthi College of Pharmacy Department of Pharmaceutical Analysis Melmaruvathur 603 319 Tamil Nadu India,
Santhi, D. V.
Adhiparasakthi College of Pharmacy Department of Pharmaceutical Analysis Melmaruvathur 603 319 Tamil Nadu India,
Vetrichelvan, T.
Adhiparasakthi College of Pharmacy Department of Pharmaceutical Analysis Melmaruvathur 603 319 Tamil Nadu India,
Shah, D. A.
Indukaka Ipcowala College of Pharmacy P.B. No. 53 New Vallabh Vidyanagar 388 121 Dist. Anand Gujarat India, dimalgroup@yahoo.com,
Bhatt, K. K.
Indukaka Ipcowala College of Pharmacy P.B. No. 53 New Vallabh Vidyanagar 388 121 Dist. Anand Gujarat India,
Baldania, S. L.
Indukaka Ipcowala College of Pharmacy P.B. No. 53 New Vallabh Vidyanagar 388 121 Dist. Anand Gujarat India,
Tian, M.
Inha University Department of Chemical Engineering 253 Yonghyun-Dong, Nam-Ku Incheon 402-751 Korea,
Li, S.
Inha University Department of Chemical Engineering 253 Yonghyun-Dong, Nam-Ku Incheon 402-751 Korea,
Row, K. H.
Inha University Department of Chemical Engineering 253 Yonghyun-Dong, Nam-Ku Incheon 402-751 Korea, rowkho@inha.ac.kr,
Azar, P. A.
Islamic Azad University, Science and Research Branch Department of Chemistry P.O. Box 14155/4933 Tehran Iran, pabroomand@yahoo.com,
Porgham-Daryasari, A.
Islamic Azad University, Science and Research Branch Department of Chemistry P.O. Box 14155/4933 Tehran Iran,
Saber-Tehrani, M.
Islamic Azad University, Science and Research Branch Department of Chemistry P.O. Box 14155/4933 Tehran Iran,
Soleimani, M.
Islamic Azad University, Lahijan Branch Department of Chemistry, Faculty of Science P.O. Box 1616 Lahijan Iran,
Patel, M. B.
Atmiya Institute of Pharmacy Department of Pharmacognosy and Phytochemistry Yogidham Gurukul, Kalawad Road Rajkot Gujarat India, mayur.pharma@gmail.com,
Mishra, S. H.
The M.S. University of Baroda Pharmacy Department, Faculty of Technology and Engineering, Kalabhavan Vadodara 390 001 Gujarat India,
Abstract : Methods based on RP-HPLC and HPTLC with UV detection for rapid quantitative determination of marker component in Enicostemma hyssopifolium, swertisin are described. The recovery of the compound was between 96.3–101.5% by HPTLC method and 98.9–100.2% by HPLC assay. The relative standard deviations ranged between 1.53–1.81 (intra-day) and 1.33–1.96 (inter-day) for HPTLC and 0.60–1.11 (intra-day) and 0.80–1.20 (inter-day) for HPLC. The methods were used for routine analysis of swertisin in the aerial parts of the plant. HPTLC method was found to be more economic and less time consuming than HPLC. Reproducibility and recovery in HPLC method were better than that in HPTLC method.

Keywords : Enicostemma hyssopifolium, swertisin, HPTLC, RP-HPLC,
Publishing house : University of Silesia in Katowice
Publication date : 2012
Number : Vol. 24, no. 1
Page : 85 – 95

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DOI :
Qute : Sajewicz, M. ,Gontarska, M. ,Kronenbach, D. ,Berry, E. ,Kowalska, T. ,Deshpande, P. B. ,Gandhi, S. V. ,Gaikwad, N. V. ,Khandagle, K. S. ,Sateesh, J. N. ,Subba Reedy, G. V. ,Jayaveera, K. N. ,Dhayalamurthi, S. ,Anandakumar, K. ,Jothieswari, D. ,Subathrai, R. ,Santhi, D. V. ,Vetrichelvan, T. ,Shah, D. A. ,Bhatt, K. K. ,Baldania, S. L. ,Tian, M. ,Li, S. ,Row, K. H. ,Azar, P. A. ,Porgham-Daryasari, A. ,Saber-Tehrani, M. ,Soleimani, M. ,Patel, M. B. ,Mishra, S. H. ,Mishra, S. H. , Quantitative analysis of marker constituent swertisin in Enicostemma hyssopifolium verdoon by RP-HPLC and HPTLC. Acta Chromatographica Vol. 24, no. 1/2012
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