Journal : Acta Chromatographica
Article : Quantitative analysis of valsartan and hydrochlorothiazide in tablets by high performance thin-layer chromatography with ultraviolet absorption densitometry

Authors :
Bartnik, M.
Department of Pharmacognosy with Medicinal Plant Laboratory, Medical University, 1, Chodźki Str., 20-093 Lublin, Poland,
Kowalski, R.
Subdepartment of Food Quality Assessment and Central Apparatus Laboratory, Agricultural University, 20-950 Lublin, Akademicka 13, Poland,
Polak, B.
Department of Physical Chemistry, Faculty of Pharmacy, Medical University, Lublin, Poland,
Turło, J.
Department of Drug Technology, Medical University of Warsaw, 1, Banacha Street, 02-097 Warsaw, Poland,
Szyrwińska, K.
Department of Inorganic and Analytical Chemistry, Faculty of Pharmacy, Poznań University of Medical Sciences, Grunwaldzka 6, 60-780 Poznań, Poland,
Czauderna, M.
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland,
Bączek, T.
Medical University of Gdańsk, Department of Biopharmaceutics and Pharmacodynamics, Gdańsk, Poland,
Chilmonczyk, Z.
National Medicines Institute, 30/34, Chełmska Str., 00-725 Warsaw, Poland,
Hung, Ch. Y.
Department of Biotechnology, National Formosa University, Huwei, Yunlin, 632, Taiwan,
Baboota, S.
Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, Hamdard Nagar, New Delhi-110062, India,
Sivakumar, T.
Department of Pharmacy, Annamalai University, Annamalai Nagar, Tamil Nadu-608 002, India,
Ivanović, D.
Faculty of Pharmacy, Department of Drug Analysis, Vojvode Stepe 450, 11000 Belgrade, Serbia,
Pavlova, V.
Institute of Chemistry, Faculty of Natural Sciences and Mathematics, Saints Cyril and Methodius University, Arhimedova 5, P.O. Box 162, 1000 Skopje, Macedonia,
Ali, J.
Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, New Delhi-110062, India,
Nabi, S. A.
Analytical Research Laboratory, Department of Chemistry, Faculty of Science, Aligarh Muslim University, Aligarh-202002, India,
Murray, K. E.
Department of Chemistry, Lafayette College, Easton, PA 18042, USA,
Antić, D.
Institute of Pharmaceutical Chemistry and Drug Analysis, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000 Belgrade, Serbia,
Jóźwiak, G. W.
Department of Inorganic Chemistry, Medical University of Lublin, Staszica 6, 20-081 Lublin, Poland,
Purghazi, K.
Department of Applied Chemistry, Faculty of Chemistry, University of Tarbiat Moallem, 49 Mofatteh Avenue, Tehran 15614, Iran,
Sajewicz, M.
Institute of Chemistry, Silesian University, 9 Szkolna Street, 40-006 Katowice, Poland,
Mohammad, A.
Analytical Research Laboratory, Department of Applied Chemistry, Faculty of Engineering and Technology, Aligarh Muslim University, Aligarh-202002, India,
Zarzycki, P. K.
Laboratory of Toxicology, Department of Environmental Biology, Koszalin University of Technology, Śniadeckich 2, 75-453 Koszalin, Poland,
Kadam, B. R.
Department of Pharmaceutical Chemistry, R.C. Patel College of Pharmacy, Karwand Naka, Shirpur, Dhule 425 405, (M.S.), India,
Abstract : A new, simple, accurate, and precise high-performance thin-layer chromatographic (HPTLC) method has been established for simultaneous analysis of valsartan and hydrochlorothiazide in tablet formulations. Standard and sample solutions of valsartan and hydrochlorothiazide were applied to precoated silica gel G 60 F254 HPTLC plates and the plates were developed with chloroform–ethyl acetate–acetic acid, 5:5:0.2 (v/v), as mobile phase. UV detection was performed densitometrically at 248 nm. The retention factors of valsartan and hydrochlorothiazide were 0.27 and 0.56, respectively. The linear range was 800–5600 ng per spot for valsartan and 125–875 ng per spot for hydrochlorothiazide; the correlation coefficients, r, were 0.9998 and 0.9988, respectively. The method was validated in accordance with the requirements of ICH guidelines and was shown to be suitable for purpose. The method was successfully used for determination of the drugs in tablets. Tablet excipients did not interfere with the chromatography.

Keywords :
Publishing house : University of Silesia in Katowice
Publication date : 2007
Number : No. 18
Page : 260 – 269

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DOI :
Qute : Bartnik, M. ,Kowalski, R. ,Polak, B. ,Turło, J. ,Szyrwińska, K. ,Czauderna, M. ,Bączek, T. ,Chilmonczyk, Z. ,Hung, Ch. Y. ,Baboota, S. ,Sivakumar, T. ,Ivanović, D. ,Pavlova, V. ,Ali, J. ,Nabi, S. A. ,Murray, K. E. ,Antić, D. ,Jóźwiak, G. W. ,Purghazi, K. ,Sajewicz, M. ,Mohammad, A. ,Zarzycki, P. K. ,Kadam, B. R. ,Kadam, B. R. , Quantitative analysis of valsartan and hydrochlorothiazide in tablets by high performance thin-layer chromatography with ultraviolet absorption densitometry. Acta Chromatographica No. 18/2007
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