RP-HPLC-PDA method development and validation for the estimation of oxcarbazepine in bulk and formulations

Czasopismo : Acta Chromatographica
Tytuł artykułu : RP-HPLC-PDA method development and validation for the estimation of oxcarbazepine in bulk and formulations

Autorzy :
Fatemi, M. H.
University of Mazandaran Laboratory of Chemometrics, Faculty of Chemistry Babolsar Iran, mhfatemi@umz.ac.ir,
Elyasi, M.
University of Mazandaran Laboratory of Chemometrics, Faculty of Chemistry Babolsar Iran,
Sowa, I.
Medical University of Lublin Department of Analytical Chemistry Chodźki 4a 20-093 Lublin Poland, i.sowa@umlub.pl,
Zajdel, D.
Medical University of Lublin Department of Analytical Chemistry Chodźki 4a 20-093 Lublin Poland,
Kocjan, R.
Medical University of Lublin Department of Analytical Chemistry Chodźki 4a 20-093 Lublin Poland,
Pizoń, M.
Medical University of Lublin Department of Analytical Chemistry Chodźki 4a 20-093 Lublin Poland,
Wójciak-Kosior, M.
Medical University of Lublin Department of Analytical Chemistry Chodźki 4a 20-093 Lublin Poland,
Hadad, G. M.
Suez Canal University Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Ismailia 41522 Egypt, ghhadad@hotmail.com,
Abdel-Salam, R. A.
Suez Canal University Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Ismailia 41522 Egypt, dr_rasalam@yahoo.com,
Abdel-Hameed, E. A.
Suez Canal University Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Ismailia 41522 Egypt,
Liu, W.-Y.
China Pharmaceutical University Department of Pharmaceutical Analysis 24 Tongjiaxiang Nanjing 210009 China, liuwenyuan8506@163.com,
Xie, S.-L.
China Pharmaceutical University Department of Pharmaceutical Analysis 24 Tongjiaxiang Nanjing 210009 China,
Xu, X. Z.
China Pharmaceutical University Department of Pharmaceutical Analysis 24 Tongjiaxiang Nanjing 210009 China,
Wu, C. Y.
China Pharmaceutical University Department of Pharmaceutical Analysis 24 Tongjiaxiang Nanjing 210009 China,
Feng, F.
China Pharmaceutical University Department of Phytochemistry 24 Tongjiaxiang Nanjing 210009 China,
Maślanka, A.
Jagiellonian University Medical College, Pharmaceutical Faculty, Department of Inorganic and Analytical Chemistry Medyczna 9 30-688 Krakow Poland,
Hubicka, U.
Jagiellonian University Medical College, Pharmaceutical Faculty, Department of Inorganic and Analytical Chemistry Medyczna 9 30-688 Krakow Poland,
Krzek, J.
Jagiellonian University Medical College, Pharmaceutical Faculty, Department of Inorganic and Analytical Chemistry Medyczna 9 30-688 Krakow Poland, jankrzek@cm-uj.krakow.pl,
Walczak, M.
Jagiellonian University Medical College, Pharmaceutical Faculty, Department of Pharmacokinetics and Physical Pharmacy Medyczna 9 30-688 Krakow Poland,
Izworski, G.
Jagiellonian University Medical College, Pharmaceutical Faculty, Department of Inorganic and Analytical Chemistry Medyczna 9 30-688 Krakow Poland,
Suchy, D.
Medical University of Silesia Department of Internal Medicine and Clinical Pharmacology Medyków 18 PL 40752 Katowice Poland, dariuszsuchy@gmail.com,
Łabuzek, K.
Medical University of Silesia Department of Internal Medicine and Clinical Pharmacology Medyków 18 PL 40752 Katowice Poland,
Pierzchała, O.
Medical University of Silesia Department of Internal Medicine and Clinical Pharmacology Medyków 18 PL 40752 Katowice Poland,
Okopień, B.
Medical University of Silesia Department of Internal Medicine and Clinical Pharmacology Medyków 18 PL 40752 Katowice Poland,
Vamsi Krishna, M.
Alliance Institute of Advanced Pharmaceutical and Health Sciences Ameerpet, Hyderabad 500038 Andhra Pradesh India, marothu_vamsi@rediffmail.com,
Dash, R. N.
Alliance Institute of Advanced Pharmaceutical and Health Sciences Ameerpet, Hyderabad 500038 Andhra Pradesh India,
Jalachandra Reddy, B.
Alliance Institute of Advanced Pharmaceutical and Health Sciences Ameerpet, Hyderabad 500038 Andhra Pradesh India,
Venugopal, P.
Alliance Institute of Advanced Pharmaceutical and Health Sciences Ameerpet, Hyderabad 500038 Andhra Pradesh India,
Sandeep, P.
Alliance Institute of Advanced Pharmaceutical and Health Sciences Ameerpet, Hyderabad 500038 Andhra Pradesh India,
Madhavi, G.
Alliance Institute of Advanced Pharmaceutical and Health Sciences Ameerpet, Hyderabad 500038 Andhra Pradesh India,
Raghavi, K.
K.V.S.R Siddhartha College of Pharmaceutical Sciences Vijayawada 520010 AP India,
Sindhura, M.
K.V.S.R Siddhartha College of Pharmaceutical Sciences Vijayawada 520010 AP India,
Prashanthi, R.
K.V.S.R Siddhartha College of Pharmaceutical Sciences Vijayawada 520010 AP India,
Nalluri, B. N.
K.V.S.R Siddhartha College of Pharmaceutical Sciences Vijayawada 520010 AP India, buchinalluri@yahoo.com,
Abstrakty : A simple, precise, rapid, and accurate liquid chromatography-mass spectrometry (LC-MS) compatible reversed phase high-performance liquid chromatography-photodiode array detection (RP-HPLC-PDA) method has been developed and validated for the estimation of oxcarbazepine (OXC) in bulk and tablet formulations. The chromatographic separation was achieved on Phenomenex C18 column (150 mm • 4.6 mm, 5.0 μm particle size) using the mobile phase comprising methanol-formic acid (0.02% v/v in water) in the ratio of 50:50 (v/v) at a flow rate of 1 mL min-1, and OXC was eluted at 6.4 min. Quantification and linearity were achieved at 229 nm over the concentration range of 10–50 μg mL-1, and the mean percentage of assay was found to be 100.03. The method was validated for specificity, linearity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ), stability, and robustness as per the International Conference on Harmonisation (ICH) guidelines and it is suitable to be employed in quality control.

Słowa kluczowe : oxcarbazepine, HPLC, validation, LC-MS compatible, Phenomenex C18 column,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2013
Numer : Vol. 25, no. 3
Strony : 519 – 529
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DOI :
Cytuj : Fatemi, M. H. ,Elyasi, M. ,Sowa, I. ,Zajdel, D. ,Kocjan, R. ,Pizoń, M. ,Wójciak-Kosior, M. ,Hadad, G. M. ,Abdel-Salam, R. A. ,Abdel-Hameed, E. A. ,Liu, W.-Y. ,Xie, S.-L. ,Xu, X. Z. ,Wu, C. Y. ,Feng, F. ,Maślanka, A. ,Hubicka, U. ,Krzek, J. ,Walczak, M. ,Izworski, G. ,Suchy, D. ,Łabuzek, K. ,Pierzchała, O. ,Okopień, B. ,Vamsi Krishna, M. ,Dash, R. N. ,Jalachandra Reddy, B. ,Venugopal, P. ,Sandeep, P. ,Madhavi, G. ,Raghavi, K. ,Sindhura, M. ,Prashanthi, R. ,Nalluri, B. N. , RP-HPLC-PDA method development and validation for the estimation of oxcarbazepine in bulk and formulations. Acta Chromatographica Vol. 25, no. 3/2013
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