Journal : Acta Chromatographica
Article : Rapid and sensitive analysis of baclofen by high-performance liquid chromatography with UV-Vis and FD detection

Authors :
Bhushan, R.
Indian Institute of Technology Roorkee Department of Chemistry Roorkee 247667 India, rbushfcy@iitr.ernet.in,
Dixit, S.
Indian Institute of Technology Roorkee Department of Chemistry Roorkee 247667 India,
Mátyus, M.
Hungarian Defence Forces Dr György Radó Military Medical Center Budapest Hungary,
Kocsis, G.
Hungarian Defence Forces Dr György Radó Military Medical Center Budapest Hungary,
Boldis, O.
Hungarian Defence Forces Dr György Radó Military Medical Center Budapest Hungary,
Karvaly, G.
Scientific Institute Military Hospital-State Health Centre P.O. Box 1 H-1553 Budapest Hungary,
Magyari, E.
Hungarian Defence Forces Dr György Radó Military Medical Center Budapest Hungary,
Fűrész, J.
Scientific Institute Military Hospital-State Health Centre P.O. Box 1 H-1553 Budapest Hungary,
Gachályi, A.
Hungarian Defence Forces Dr György Radó Military Medical Center Budapest Hungary,
Mantovani, L.
Universidade Federal do Pampa Laboratório de Pesquisa e Desenvolvimento em Controle de Qualidade de Medicamentos BR 472 km 585 Prédio Administrativo CEP 97500-970 Uruguaiana-RS Brazil,
Sayago, C. T. M.
Universidade Federal do Pampa Laboratório de Pesquisa e Desenvolvimento em Controle de Qualidade de Medicamentos BR 472 km 585 Prédio Administrativo CEP 97500-970 Uruguaiana-RS Brazil,
Camargo, V. B.
Universidade Federal do Pampa Laboratório de Pesquisa e Desenvolvimento em Controle de Qualidade de Medicamentos BR 472 km 585 Prédio Administrativo CEP 97500-970 Uruguaiana-RS Brazil,
Silveira, V. F.
Universidade Federal do Pampa Laboratório de Pesquisa e Desenvolvimento em Controle de Qualidade de Medicamentos BR 472 km 585 Prédio Administrativo CEP 97500-970 Uruguaiana-RS Brazil,
Garcia, C. V.
Universidade Federal do Rio Grande do Sul Programa de Pós-graduação em Ciências Farmacêuticas Av. Ipiranga 2752 Lab 402 CEP 90610-000 Porto Alegre-RS Brazil,
Schapoval, E. E. S.
Universidade Federal do Rio Grande do Sul Programa de Pós-graduação em Ciências Farmacêuticas Av. Ipiranga 2752 Lab 402 CEP 90610-000 Porto Alegre-RS Brazil,
Mendez, A. S. L.
Universidade Federal do Pampa Laboratório de Pesquisa e Desenvolvimento em Controle de Qualidade de Medicamentos BR 472 km 585 Prédio Administrativo CEP 97500-970 Uruguaiana-RS Brazil, andreaslmendez@yahoo.com.br,
Cao, L.-W.
Jinan University Department of Chemistry Guangzhou 510632 People’s Republic of China, lwcao@126.com,
Li, C.
Jinan University Department of Chemistry Guangzhou 510632 People’s Republic of China,
Abstract : Two rapid, sensitive and reproducible methods for the determination of baclofen( BAL) in urine and plasma based on high-performance liquid chromatography (HPLC) with UV-vis and fluorescent detection, respectively, were developed for the first time using a new synthesized fluorescent label, 6-oxy-(N-succinimidylacetate)-9-(2′-methoxycarbonyl) fluorescein (SAMF). The optimal derivatization yield was achieved in borate buffer (pH 8.0) for 15 min at room temperature (25 °C). With a mixture of methanol and water containing 5 mmol L-1sodium citrate buffer (pH 5.0) as mobile phase, BAL was determined at λ = 455 nm with UV-vis detection and at λex/λem = 488/520 nm with FD detection. The detection limits are 1.065 × 10-3mg mL-1and 1.065 × 10-2mg mL-1with HPLC-UV-vis and HPLC-FD, respectively. The proposed method has been successfully applied to the analysis of BAL in human urine and plasma samples. The established method is rapid (15 min of derivatization process and 10 min of chromatographic run), reproducible and sensitive.

Keywords : baclofen, 6-oxy-(N-succinimidyl acetate)-9-(2′-methoxycarbonyl) fluorescein, high-performance liquid chromatography, UV-vis detection, fluorescent detection,
Publishing house : University of Silesia in Katowice
Publication date : 2012
Number : Vol. 24, no. 3
Page : 383 – 397

Bibliography
: 1 H.Y. Aboul-Enein and I.W. Wainer, The Impact of Stereochemistry on Drug Development and Use, John Wiley & Sons, New York, NY, 1997, p. 58
2 R.I. Stefan van Staden and A.A Rat’k. Talanta, 69, 1049–1053 (2006)
3 S.J Hosseinimehr, F. Pourmorad, E. Moshtaghi, and M. Amini, Asian J. Che, 22, 522–526 (2010)
4 H.M. Saleh, M.M. EL-Henawee, G.H. Ragab, and S.S. El-Hay Abd, Spectrochim. Acta, Part A, 67, 1284–1289 (2007)
5 A. Rat’ko and R. Stefan-van Staden, IL FARMACO, 59, 993–997 (2004)
6 R. Bhushan and V. Kumar, Chromatographia, 22, 906–911 (2008)
7 A. Siouffi, G. Kaiser, and F. Leroux, J. Chromatogr., 450, 221–232 (1988)
8 M.M. Hefnawy and H.Y. Aboul-Enein, Talanta, 61, 667–673 (2003)
9 C.Y. Zhang, M. Yuan, B.Y. Huang, H. Ji, and L. Zhu, West Chin. J. Pharm. Sci., 23, 342–343 (2008)
10 S. Tosunoglu and L. Ersoy, Analyst, 120, 373–375 (1995)
11 G. Kavran-Belin, S. Rudaz, and J.L. Veuthey, J. Sep. Sci., 28, 2187–2192 (2005)
12 Y.S. Gu and C.W. Whang, J. Chromatogr. A, 972, 289–293 (2002)
13 Miksa and R. Poppenga, J. Anal. Toxicol., 27, 275–283 (2004)
14 L. Millerioux, M. Brault, V. Gualano, and A. Mignot, J. Chromatogr. A, 729, 309–314 (1996)
15 S. Chang and H. Yang, Chromatographia, 57, 825–829 (2003)
16 M.T. Chiang, S.Y. Chang and C.W. Whang, J. Chromatogr. A, 877, 233–237 (2000)
17 L.W. Cao, H.S. Zhang, and H. Wang, Microchim. Acta, 158, 361–368 (2007)
18 L.W. Cao, H. Wang, J.S. Li, and H.S. Zhang, J. Chromatogr. A, 1063, 143–151 (2005)
19 V.C. Wu, S.L. Lin, S.M. Lin, and C.C. Fang, Nephrol. Dial. Transpl., 20, 441–443 (2005)
20 R.P. Haugl, The Handbook. A Guide to Fluorescent Probes and Labeling Technologies, 10th edn, Molecular Probes Inc, Eugene, OR, 2005, p. 1
DOI :
Qute : Bhushan, R. ,Dixit, S. ,Mátyus, M. ,Kocsis, G. ,Boldis, O. ,Karvaly, G. ,Magyari, E. ,Fűrész, J. ,Gachályi, A. ,Mantovani, L. ,Sayago, C. T. M. ,Camargo, V. B. ,Silveira, V. F. ,Garcia, C. V. ,Schapoval, E. E. S. ,Mendez, A. S. L. ,Cao, L.-W. ,Li, C. ,Li, C. , Rapid and sensitive analysis of baclofen by high-performance liquid chromatography with UV-Vis and FD detection. Acta Chromatographica Vol. 24, no. 3/2012
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