Rapid extraction, isolation, and quantification of oleanolic acid from Lantana camara L. Roots using microwave and HPLC-PDA techniques

Czasopismo : Acta Chromatographica
Tytuł artykułu : Rapid extraction, isolation, and quantification of oleanolic acid from Lantana camara L. Roots using microwave and HPLC-PDA techniques

Autorzy :
Petruczynik, A.
Medical University of Lublin Department of Inorganic Chemistry 4a Chodźki Str. 20-093 Lublin Poland, annapetruczynik@poczta.onet.pl,
Waksmundzka-Hajnos, M.
Medical University of Lublin Department of Inorganic Chemistry 4a Chodźki Str. 20-093 Lublin Poland,
Rykowska, I.
Adam Mickiewicz University Faculty of Chemistry Umultowska 89b 61-614 Poznań Poland, obstiwo@amu.edu.pl,
Wasiak, W.
Adam Mickiewicz University Faculty of Chemistry Umultowska 89b 61-614 Poznań Poland,
Inglot, T. W.
Medical University of Lublin Department of Medicinal Chemistry, Faculty of Pharmacy Jaczewskiego 4 20-090 Lublin Poland,
Gumieniczek, A.
anna.gumieniczek@am.lublin.pl,
Komsta, Ł.
Medical University of Lublin Department of Medicinal Chemistry, Faculty of Pharmacy Jaczewskiego 4 20-090 Lublin Poland,
Związek, R.
Medical University of Lublin Department of Medicinal Chemistry, Faculty of Pharmacy Jaczewskiego 4 20-090 Lublin Poland,
Sharaf El Din, M. K.
Mansoura University Department of Analytical Chemistry, Faculty of Pharmacy Mansoura 35516 Egypt,
Ibrahim, F.
Mansoura University Department of Analytical Chemistry, Faculty of Pharmacy Mansoura 35516 Egypt,
Eid, M. I.
Mansoura University Department of Analytical Chemistry, Faculty of Pharmacy Mansoura 35516 Egypt,
Wahba, M. E. K.
Mansoura University Department of Analytical Chemistry, Faculty of Pharmacy Mansoura 35516 Egypt, marywahba2004@yahoo.com,
El-Shaboury, S. R.
Assiut University Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Assiut 71526 Egypt,
Hussein, A. S.
Assiut University Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Assiut 71526 Egypt,
Mohamed, N. A.
Assiut University Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Assiut 71526 Egypt, mohamed_mounir2000@yahoo.com,
El-Sutohy, M. M.
Al-Azhar University, Assiut Branch Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Assiut 71524 Egypt,
Madej, K.
Jagiellonian University Department of Analytical Chemistry, Faculty of Chemistry Ingardena St. 3 30-060 Krakow Poland, madejk@chemia.uj.edu.pl,
Persona, K.
Jagiellonian University Department of Analytical Chemistry, Faculty of Chemistry Ingardena St. 3 30-060 Krakow Poland,
Nizio, M.
Jagiellonian University Department of Analytical Chemistry, Faculty of Chemistry Ingardena St. 3 30-060 Krakow Poland,
Emami, J.
Isfahan University of Medical Sciences Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences Isfahan Iran, emami@pharm.mui.ac.ir,
Rezazadeh, M.
Isfahan University of Medical Sciences Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences Isfahan Iran,
Smolarz, H. D.
Medical University of Lublin Chair and Department of Pharmaceutical Botany Lublin Poland, h.smolarz@am.lublin.pl,
Hałka, A.
Medical University of Lublin Department of Physical Chemistry, Chair of Chemistry Lublin Poland,
Chabros, O.
Medical University of Lublin Chair and Department of Pharmaceutical Botany Lublin Poland,
Dzido, T. H.
Medical University of Lublin Department of Physical Chemistry, Chair of Chemistry Lublin Poland,
Rode, S.
Saraswati Vidya Bhawan’s College of Pharmacy Sonarpada, Dombivli (East) 421 204 Maharashtra India,
Bhujbal, P.
Saraswati Vidya Bhawan’s College of Pharmacy Sonarpada, Dombivli (East) 421 204 Maharashtra India,
Sandhya, P.
Saraswati Vidya Bhawan’s College of Pharmacy Sonarpada, Dombivli (East) 421 204 Maharashtra India, iamsandhyap@yahoo.com,
Zhu, F. X.
Jiangsu Provincial Academy of Chinese Medicine Key Laboratory of New Drug Delivery System of Chinese Meteria Medica No. 100 Shizi Road Nanjing 210028 China,
Jia, X. B.
Jiangsu Provincial Academy of Chinese Medicine Key Laboratory of New Drug Delivery System of Chinese Meteria Medica No. 100 Shizi Road Nanjing 210028 China,
Cai, Y.
Jiangsu Provincial Academy of Chinese Medicine Key Laboratory of New Drug Delivery System of Chinese Meteria Medica No. 100 Shizi Road Nanjing 210028 China,
Chen, Y.
Jiangsu Provincial Academy of Chinese Medicine Key Laboratory of New Drug Delivery System of Chinese Meteria Medica No. 100 Shizi Road Nanjing 210028 China,
Bhope, S. G.
MIDC Ranjangaon Analytical R&D, Tulip Lab Pvt. Ltd. F-20/21 Pune 412220 India, bshrinivas16@gmail.com,
Kuber, V. V.
MIDC Ranjangaon Analytical R&D, Tulip Lab Pvt. Ltd. F-20/21 Pune 412220 India,
Nagore, D. H.
MIDC Ranjangaon Analytical R&D, Tulip Lab Pvt. Ltd. F-20/21 Pune 412220 India,
Gaikwad, P. S.
MIDC Ranjangaon Analytical R&D, Tulip Lab Pvt. Ltd. F-20/21 Pune 412220 India,
Patil, M. J.
Marathwada Mitra Mandal College of Pharmacy Department of Pharmacognosy Pune India,
Liao, Z. X.
Southeast University Department of Pharmaceutical Engineering, School of Chemistry and Chemical Engineering Nanjing 211189 China,
Zhang, B. B.
Southeast University Department of Pharmaceutical Engineering, School of Chemistry and Chemical Engineering Nanjing 211189 China,
Ding, L. S.
Chinese Academy of Sciences Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization, Chengdu Institute of Biology Chengdu 610041 China,
Zhou, Y.
Chinese Academy of Sciences Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization, Chengdu Institute of Biology Chengdu 610041 China, zhouyan@cib.ac.cn,
Verma, S. C.
Central Council for Research in Ayurvedic Sciences 61-65, Institutional Area, Opp.-D-Block, Janakpuri New Delhi 110058 India, scvpharma@gmail.com,
Jain, C. L.
M. M. H. College Department of Chemistry Ghaziabad U.P. India,
Nigam, S.
Central Council for Research in Ayurvedic Sciences 61-65, Institutional Area, Opp.-D-Block, Janakpuri New Delhi 110058 India,
Padhi, M. M.
Central Council for Research in Ayurvedic Sciences 61-65, Institutional Area, Opp.-D-Block, Janakpuri New Delhi 110058 India,
Abstrakty : An ecofriendly solvent polarity based microwave-assisted extraction (MAE) technique was developed for the rapid extraction and isolation of bioactive oleanolic acid from roots of Lantana camara L. Several different influential extraction parameters such as microwave power, extraction time, solvent type, and volume were studied in a systematic fashion for the determination of optimum extraction conditions. Simply modified and rapid high-performance liquid chromatography-diode array detector (HPLC-DAD) method was also developed and validated for quantitative determination of oleanolic acid from roots of L. camara. Under optimum conditions, using a mixture of CHCl3:MeOH (60:40, v/v, 15 mL) as a solvent, 600 W microwave powers, and 50 °C temperature for 6 min of MAE produced a maximum yield of 1.23% (dry weight of roots). No degradation of the target analyte was observed at the optimum conditions as evidenced from the recovery studies performed with standard oleanolic acid. The proposed method also showed high degree of reproducibility; hence, it may be useful for maximum extraction and isolation of biologically active oleanolic acid.

Słowa kluczowe : Lantana camara L, roots, microwave-assisted extraction and isolation, oleanolic acid, HPLC-PDA method,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2013
Numer : Vol. 25, no. 1
Strony : 181 – 199
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DOI :
Cytuj : Petruczynik, A. ,Waksmundzka-Hajnos, M. ,Rykowska, I. ,Wasiak, W. ,Inglot, T. W. ,Gumieniczek, A. ,Komsta, Ł. ,Związek, R. ,Sharaf El Din, M. K. ,Ibrahim, F. ,Eid, M. I. ,Wahba, M. E. K. ,El-Shaboury, S. R. ,Hussein, A. S. ,Mohamed, N. A. ,El-Sutohy, M. M. ,Madej, K. ,Persona, K. ,Nizio, M. ,Emami, J. ,Rezazadeh, M. ,Smolarz, H. D. ,Hałka, A. ,Chabros, O. ,Dzido, T. H. ,Rode, S. ,Bhujbal, P. ,Sandhya, P. ,Zhu, F. X. ,Jia, X. B. ,Cai, Y. ,Chen, Y. ,Bhope, S. G. ,Kuber, V. V. ,Nagore, D. H. ,Gaikwad, P. S. ,Patil, M. J. ,Liao, Z. X. ,Zhang, B. B. ,Ding, L. S. ,Zhou, Y. ,Verma, S. C. ,Jain, C. L. ,Nigam, S. ,Padhi, M. M. , Rapid extraction, isolation, and quantification of oleanolic acid from Lantana camara L. Roots using microwave and HPLC-PDA techniques. Acta Chromatographica Vol. 25, no. 1/2013
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