Rapid method for rhaponticin and deoxyrhaponticin separation and determination by TLC in Rheum rhaponticum L. and Rheum undulatum L.

Czasopismo : Acta Chromatographica
Tytuł artykułu : Rapid method for rhaponticin and deoxyrhaponticin separation and determination by TLC in Rheum rhaponticum L. and Rheum undulatum L.

Autorzy :
Petruczynik, A.
Medical University of Lublin Department of Inorganic Chemistry 4a Chodźki Str. 20-093 Lublin Poland, annapetruczynik@poczta.onet.pl,
Waksmundzka-Hajnos, M.
Medical University of Lublin Department of Inorganic Chemistry 4a Chodźki Str. 20-093 Lublin Poland,
Rykowska, I.
Adam Mickiewicz University Faculty of Chemistry Umultowska 89b 61-614 Poznań Poland, obstiwo@amu.edu.pl,
Wasiak, W.
Adam Mickiewicz University Faculty of Chemistry Umultowska 89b 61-614 Poznań Poland,
Inglot, T. W.
Medical University of Lublin Department of Medicinal Chemistry, Faculty of Pharmacy Jaczewskiego 4 20-090 Lublin Poland,
Gumieniczek, A.
anna.gumieniczek@am.lublin.pl,
Komsta, Ł.
Medical University of Lublin Department of Medicinal Chemistry, Faculty of Pharmacy Jaczewskiego 4 20-090 Lublin Poland,
Związek, R.
Medical University of Lublin Department of Medicinal Chemistry, Faculty of Pharmacy Jaczewskiego 4 20-090 Lublin Poland,
Sharaf El Din, M. K.
Mansoura University Department of Analytical Chemistry, Faculty of Pharmacy Mansoura 35516 Egypt,
Ibrahim, F.
Mansoura University Department of Analytical Chemistry, Faculty of Pharmacy Mansoura 35516 Egypt,
Eid, M. I.
Mansoura University Department of Analytical Chemistry, Faculty of Pharmacy Mansoura 35516 Egypt,
Wahba, M. E. K.
Mansoura University Department of Analytical Chemistry, Faculty of Pharmacy Mansoura 35516 Egypt, marywahba2004@yahoo.com,
El-Shaboury, S. R.
Assiut University Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Assiut 71526 Egypt,
Hussein, A. S.
Assiut University Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Assiut 71526 Egypt,
Mohamed, N. A.
Assiut University Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Assiut 71526 Egypt, mohamed_mounir2000@yahoo.com,
El-Sutohy, M. M.
Al-Azhar University, Assiut Branch Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Assiut 71524 Egypt,
Madej, K.
Jagiellonian University Department of Analytical Chemistry, Faculty of Chemistry Ingardena St. 3 30-060 Krakow Poland, madejk@chemia.uj.edu.pl,
Persona, K.
Jagiellonian University Department of Analytical Chemistry, Faculty of Chemistry Ingardena St. 3 30-060 Krakow Poland,
Nizio, M.
Jagiellonian University Department of Analytical Chemistry, Faculty of Chemistry Ingardena St. 3 30-060 Krakow Poland,
Emami, J.
Isfahan University of Medical Sciences Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences Isfahan Iran, emami@pharm.mui.ac.ir,
Rezazadeh, M.
Isfahan University of Medical Sciences Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences Isfahan Iran,
Smolarz, H. D.
Medical University of Lublin Chair and Department of Pharmaceutical Botany Lublin Poland, h.smolarz@am.lublin.pl,
Hałka, A.
Medical University of Lublin Department of Physical Chemistry, Chair of Chemistry Lublin Poland,
Chabros, O.
Medical University of Lublin Chair and Department of Pharmaceutical Botany Lublin Poland,
Dzido, T. H.
Medical University of Lublin Department of Physical Chemistry, Chair of Chemistry Lublin Poland,
Abstrakty : The study describes the solvent extraction procedure and improved version of TLC method for the determination of rhaponticin and deoxyrhaponticin in rhizomes and petioles of two species of Rheum L. genus. Extracts from underground parts and petioles of plant were separated on thin layers of silica using a mixture of dichloromethane-95% ethanol-methanol (8:1:1). The bands of rhaponticin and deoxyrhaponticin were satisfactorily separated from the remaining extract components permitting their densitometric quantitative and qualitative determination. The concentration of these compounds in Rheum undulatum was higher than in Rheum rhaponticum. The amount of rhaponticin and deoxyrhaponticin ranged between 0.3 and 31.5 mg/g.

Słowa kluczowe : planar chromatography, extraction, rheum, rhaponticin, deoxyrhaponticin,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2013
Numer : Vol. 25, no. 1
Strony : 127 – 134
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DOI :
Cytuj : Petruczynik, A. ,Waksmundzka-Hajnos, M. ,Rykowska, I. ,Wasiak, W. ,Inglot, T. W. ,Gumieniczek, A. ,Komsta, Ł. ,Związek, R. ,Sharaf El Din, M. K. ,Ibrahim, F. ,Eid, M. I. ,Wahba, M. E. K. ,El-Shaboury, S. R. ,Hussein, A. S. ,Mohamed, N. A. ,El-Sutohy, M. M. ,Madej, K. ,Persona, K. ,Nizio, M. ,Emami, J. ,Rezazadeh, M. ,Smolarz, H. D. ,Hałka, A. ,Chabros, O. ,Dzido, T. H. , Rapid method for rhaponticin and deoxyrhaponticin separation and determination by TLC in Rheum rhaponticum L. and Rheum undulatum L.. Acta Chromatographica Vol. 25, no. 1/2013
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