Journal : Acta Chromatographica
Article : Rapid profiling of crude scorpion venom using liquid chromatography and its relevance to species identification

Authors :
Bhushan, R.
Indian Institute of Technology Roorkee Department of Chemistry Roorkee 247667 India, rbushfcy@iitr.ernet.in,
Dixit, S.
Indian Institute of Technology Roorkee Department of Chemistry Roorkee 247667 India,
Mátyus, M.
Hungarian Defence Forces Dr György Radó Military Medical Center Budapest Hungary,
Kocsis, G.
Hungarian Defence Forces Dr György Radó Military Medical Center Budapest Hungary,
Boldis, O.
Hungarian Defence Forces Dr György Radó Military Medical Center Budapest Hungary,
Karvaly, G.
Scientific Institute Military Hospital-State Health Centre P.O. Box 1 H-1553 Budapest Hungary,
Magyari, E.
Hungarian Defence Forces Dr György Radó Military Medical Center Budapest Hungary,
Fűrész, J.
Scientific Institute Military Hospital-State Health Centre P.O. Box 1 H-1553 Budapest Hungary,
Gachályi, A.
Hungarian Defence Forces Dr György Radó Military Medical Center Budapest Hungary,
Mantovani, L.
Universidade Federal do Pampa Laboratório de Pesquisa e Desenvolvimento em Controle de Qualidade de Medicamentos BR 472 km 585 Prédio Administrativo CEP 97500-970 Uruguaiana-RS Brazil,
Sayago, C. T. M.
Universidade Federal do Pampa Laboratório de Pesquisa e Desenvolvimento em Controle de Qualidade de Medicamentos BR 472 km 585 Prédio Administrativo CEP 97500-970 Uruguaiana-RS Brazil,
Camargo, V. B.
Universidade Federal do Pampa Laboratório de Pesquisa e Desenvolvimento em Controle de Qualidade de Medicamentos BR 472 km 585 Prédio Administrativo CEP 97500-970 Uruguaiana-RS Brazil,
Silveira, V. F.
Universidade Federal do Pampa Laboratório de Pesquisa e Desenvolvimento em Controle de Qualidade de Medicamentos BR 472 km 585 Prédio Administrativo CEP 97500-970 Uruguaiana-RS Brazil,
Garcia, C. V.
Universidade Federal do Rio Grande do Sul Programa de Pós-graduação em Ciências Farmacêuticas Av. Ipiranga 2752 Lab 402 CEP 90610-000 Porto Alegre-RS Brazil,
Schapoval, E. E. S.
Universidade Federal do Rio Grande do Sul Programa de Pós-graduação em Ciências Farmacêuticas Av. Ipiranga 2752 Lab 402 CEP 90610-000 Porto Alegre-RS Brazil,
Mendez, A. S. L.
Universidade Federal do Pampa Laboratório de Pesquisa e Desenvolvimento em Controle de Qualidade de Medicamentos BR 472 km 585 Prédio Administrativo CEP 97500-970 Uruguaiana-RS Brazil, andreaslmendez@yahoo.com.br,
Cao, L.-W.
Jinan University Department of Chemistry Guangzhou 510632 People’s Republic of China, lwcao@126.com,
Li, C.
Jinan University Department of Chemistry Guangzhou 510632 People’s Republic of China,
Desai, D.
The Maharaja Sayajirao University of Baroda Quality Assurance Laboratory, Centre of Relevance and Excellence in Novel Drug Delivery System, Pharmacy Department, G. H. Patel Pharmacy Building, Donor’s Plaza Fatehgunj, Vadodara 390 002 Gujarat India,
Patel, G.
The Maharaja Sayajirao University of Baroda Quality Assurance Laboratory, Centre of Relevance and Excellence in Novel Drug Delivery System, Pharmacy Department, G. H. Patel Pharmacy Building, Donor’s Plaza Fatehgunj, Vadodara 390 002 Gujarat India,
Shukla, N.
The Maharaja Sayajirao University of Baroda Quality Assurance Laboratory, Centre of Relevance and Excellence in Novel Drug Delivery System, Pharmacy Department, G. H. Patel Pharmacy Building, Donor’s Plaza Fatehgunj, Vadodara 390 002 Gujarat India,
Rajput, S.
The Maharaja Sayajirao University of Baroda Quality Assurance Laboratory, Centre of Relevance and Excellence in Novel Drug Delivery System, Pharmacy Department, G. H. Patel Pharmacy Building, Donor’s Plaza Fatehgunj, Vadodara 390 002 Gujarat India, sjrajput@gmail.com,
Joshi, S. S.
National Institute of Pharmaceutical Education and Research (NIPER) Balanagar, Hyderabad 500037 India,
Nahire, R. R.
National Institute of Pharmaceutical Education and Research (NIPER) Balanagar, Hyderabad 500037 India,
Shastri, N. R.
National Institute of Pharmaceutical Education and Research (NIPER) Balanagar, Hyderabad 500037 India, svcphod@yahoo.co.in,
Surendranath, K. V.
ICICI Knowledge Park United States Pharmacopeia India Private Limited, Reference Standard Laboratory Shameerpet, Hyderabad 500078 India,
Satish, J.
ICICI Knowledge Park United States Pharmacopeia India Private Limited, Reference Standard Laboratory Shameerpet, Hyderabad 500078 India,
Kobyłka, M.
Medical University of Lublin Department of Medicinal Chemistry Jaczewskiego 4 20-090 Lublin Poland,
Komsta, Ł.
Medical University of Lublin Department of Medicinal Chemistry Jaczewskiego 4 20-090 Lublin Poland, lukasz.komsta@umlub.pl,
Adegoke, O. A.
University of Ibadan Department of Pharmaceutical Chemistry, Faculty of Pharmacy Ibadan Nigeria, ao.adegoke@mail.ui.edu.ng,
Xiang, L. L.
Chinese Academy of Sciences Laboratory of Pharmaceutical Analysis, Shanghai Institute of Materia Medica 555 Zuchongzhi Road Shanghai 201203 PR China,
Idowu, O. S.
University of Ibadan Department of Pharmaceutical Chemistry, Faculty of Pharmacy Ibadan Nigeria,
Chen, D.-Y.
Chinese Academy of Sciences Laboratory of Pharmaceutical Analysis, Shanghai Institute of Materia Medica 555 Zuchongzhi Road Shanghai 201203 PR China, dychen@mail.shcnc.ac.cn,
Omar, A. A.
Alexandria University Department of Pharmacognosy, Faculty of Pharmacy Alexandria 21521 Egypt,
Hadad, G. M.
Suez Canal University Department of Analytical Chemistry, Faculty of Pharmacy Ismailia 41522 Egypt,
Badr, J. M.
King Abdulaziz University Department of Natural Products and Alternative Medicine, Faculty of Pharmacy Jeddah 21589 Kingdom of Saudi Arabia, jihanbadr2010@hotmail.com,
Chen, Y.
Southern Medical University Key Laboratory of Chinese Drugs Pharmaceutics of Guang Dong Province, School of Traditional Chinese Medicine 510515 Guangzhou People’s Republic of China,
Li, X.
Chifeng Aike Pharmaceutical Technology Co., Ltd. 024001 Chifeng People’s Republic of China,
Chen, F.
Southern Medical University Key Laboratory of Chinese Drugs Pharmaceutics of Guang Dong Province, School of Traditional Chinese Medicine 510515 Guangzhou People’s Republic of China,
Zhu, Q.
Southern Medical University Key Laboratory of Chinese Drugs Pharmaceutics of Guang Dong Province, School of Traditional Chinese Medicine 510515 Guangzhou People’s Republic of China,
Chen, J.
Chinese Academy of Sciences Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P. R. China,
Shi, Y. P.
Chinese Academy of Sciences Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P. R. China, shiyp@licp.cas.cn,
Asmari, A.
Armed Forces Hospital Research Center Riyadh Saudi Arabia, Rkh_research@yahoo.com,
Khan, H. A.
King Saud University Prince Sultan Research Chair for Environment and Wildlife, Department of Botany and Microbiology, College of Science Riyadh Saudi Arabia,
Manthiri, R. A.
Armed Forces Hospital Research Center Riyadh Saudi Arabia,
Abstract : We present the comprehensive chromatographic profiles of three scorpion species, Androctonus crassicauda, Androctonus bicolor, and Leiurus quinquestriatus, commonly inhabited to Middle East regions. Their venoms were milked by electrical stimulation, diluted with distilled water, properly mixed and centrifuged to separate the mucus from venom. The clear supernatant was filtered and the protein concentration was determined. Pre-diluted venoms were chromatographed on FPLC (fast protein liquid chromatography) and RP-HPLC (reversed-phase high-performance liquid chromatography) and the fractions were collected for molecular weight determination. Both techniques have resulted clearly distinguishable chromatographic patterns that can be used for identification of scorpion species and having a quick indication of venom toxicity.

Keywords : scorpion venom, chromatography, venom profile, FPLC, HPLC, toxicity, forensic,
Publishing house : University of Silesia in Katowice
Publication date : 2012
Number : Vol. 24, no. 3
Page : 501 – 509

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DOI :
Qute : Bhushan, R. ,Dixit, S. ,Mátyus, M. ,Kocsis, G. ,Boldis, O. ,Karvaly, G. ,Magyari, E. ,Fűrész, J. ,Gachályi, A. ,Mantovani, L. ,Sayago, C. T. M. ,Camargo, V. B. ,Silveira, V. F. ,Garcia, C. V. ,Schapoval, E. E. S. ,Mendez, A. S. L. ,Cao, L.-W. ,Li, C. ,Desai, D. ,Patel, G. ,Shukla, N. ,Rajput, S. ,Joshi, S. S. ,Nahire, R. R. ,Shastri, N. R. ,Surendranath, K. V. ,Satish, J. ,Kobyłka, M. ,Komsta, Ł. ,Adegoke, O. A. ,Xiang, L. L. ,Idowu, O. S. ,Chen, D.-Y. ,Omar, A. A. ,Hadad, G. M. ,Badr, J. M. ,Chen, Y. ,Li, X. ,Chen, F. ,Zhu, Q. ,Chen, J. ,Shi, Y. P. ,Asmari, A. ,Khan, H. A. ,Manthiri, R. A. ,Manthiri, R. A. , Rapid profiling of crude scorpion venom using liquid chromatography and its relevance to species identification. Acta Chromatographica Vol. 24, no. 3/2012
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