Journal : Acta Chromatographica
Article : Separation and analysis of phenylethanoid glycosides in Lamiophlomis rotata by high-performance liquid chromatography

Authors :
Huo, H. L.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Yu, S. H.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Liu, X. Z.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Meng, Y.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Ren, Y. P.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Zhang, L. T.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China, zhanglantong@263.net,
Liu, L.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Zhao, T.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Cheng, X. M.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Wang, C. H.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China, wchcxm@hotmail.com,
Wang, Z. T.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China, wangzt@hotmail.com,
Gao, X.
Sichuan University Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy Chengdu, Sichuan China, gaomuxouzi@126.com,
Jiang, X.
Sichuan University Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy Chengdu, Sichuan China,
Wang, L.
Sichuan University Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy Chengdu, Sichuan China,
Urban, J.
University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, School of Complex Systems (former Institute of Physical Biology), Laboratory of Applied System Biology Zamek 136 Nove Hrady 373 33 Czech Republic, urbanj@frov.jcu.cz,
Vaněk, J.
University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, School of Complex Systems (former Institute of Physical Biology), Laboratory of Applied System Biology Zamek 136 Nove Hrady 373 33 Czech Republic,
Štys, D.
University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, School of Complex Systems (former Institute of Physical Biology), Laboratory of Applied System Biology Zamek 136 Nove Hrady 373 33 Czech Republic,
Li, C. D.
Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P.R. China,
Liu, Y.
Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P.R. China,
Shi, Y. P.
Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P.R. China, shiyp@licp.cas.cn,
Zhang, Y.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China, hangsong@vip.sina.com,
Peng, Q.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Zeng, H.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Yao, S.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Zhang, Y.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Song, H.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Zhou, W.
Guiyang Medical University Department of Pharmaceutical Analysis, School of Pharmacy Guiyang 550004 China,
Zhang, X. Y.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China, drxyzhang@126.com,
Lv, Y. P.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China,
Liu, X. D.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China,
Xu, C.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China,
Duan, G. L.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China, glduan@shmu.edu.cn,
Smoliński, A.
Central Mining Institute Department of Energy Saving and Air Protection Sq. Gwarkow 1 40-166 Katowice, Poland, smolin@gig.katowice.pl,
Howaniec, N.
Central Mining Institute Department of Energy Saving and Air Protection Sq. Gwarkow 1 40-166 Katowice, Poland,
Unver-Somer, N.
Ege University Faculty of Pharmacy, Department of Pharmacognosy 35100 Bornova, Izmir Turkey,
Cicek-Polat, D.
Ege University Faculty of Pharmacy, Department of Pharmacognosy 35100 Bornova, Izmir Turkey,
Emir, A.
Ege University Faculty of Pharmacy, Department of Pharmacognosy 35100 Bornova, Izmir Turkey,
Onur, M. A.
Ege University Faculty of Pharmacy, Department of Pharmacognosy 35100 Bornova, Izmir Turkey,
Kaya, G. I.
Ege University Faculty of Pharmacy, Department of Pharmacognosy 35100 Bornova, Izmir Turkey,
Ahmed, A. B. A.
Bharathidasan University Department of Plant Science Tiruchirappalli 620 024 Tamil Nadu India, dr.bakrudeenaliahmed@yahoo.co.in,
Rao, A. S.
Bharathidasan University Department of Biotechnology Tiruchirappalli 620 024 Tamil Nadu India,
Rao, M. V.
Bharathidasan University Department of Plant Science Tiruchirappalli 620 024 Tamil Nadu India,
Taha, R. M.
University of Malaya Institute of Biological Sciences, Faculty of Science 50603 Kuala Lumpur Malaysia,
Choi, Y.
Sungkyunkwan University School of Pharmacy Suwon, Gyeonggi-do Korea,
Shin, B. S.
Catholic University of Daegu College of Pharmacy Gyeongsan-si, Gyeongbuk Korea,
Kim, M. G.
Sungkyunkwan University School of Pharmacy Suwon, Gyeonggi-do Korea,
Shin, S. W.
Sungkyunkwan University School of Pharmacy Suwon, Gyeonggi-do Korea,
Kim, T. H.
Catholic University of Daegu College of Pharmacy Gyeongsan-si, Gyeongbuk Korea,
Jang, H. C.
Sungkyunkwan University School of Pharmacy Suwon, Gyeonggi-do Korea,
Yoo, S. D.
Sungkyunkwan University School of Pharmacy Suwon, Gyeonggi-do Korea, sdyoo@skku.ac.kr,
Dong, S.
The Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 China, sqdong@licp.cas.cn,
Shi, X.
Gansu Academy of Medical Sciences Lanzhou 730000 China,
Liu, Q.
Graduate University of the Chinese Academy of Sciences Beijing 100039 China,
Zhang, Z.
The Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 China,
Zhao, L.
The Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 China,
Abstract : A simple hydrolysis method has been developed for determination of phenylethanoid glycosides in Lamiophlomis rotata (L.R.). Different kinds of phenylethanoid glycosides were hydrolyzed in hydrochloric acid solution to produce corresponding phenethyl alcohols and cinnamic acids, mainly containing hydroxytyrosol, homovanillyl alcohol, 3,4-dimethoxyphenethyl alcohol, caffeic acid, fumalic acid and 3,4-dimethoxycinnamic acid. The six analytes could be determined simultaneously by high-performance liquid chromatography (HPLC). The effects of mobile phase, pH and concentration of running buffer, detection wavelength, flow rate and injection volume were also investigated. Under the optimum conditions, the six hydrolyzates could be perfectly separated within 45 min. The response was linear over four orders of magnitude with detection limits (S/N = 3) ranging from 1 × 10-8to 1.5 × 10-4mol L-1for the analytes. The method has been successfully applied to the analysis of real sample Du-Yi-Wei capsule and Qi-Zheng-Yan-Tong patch, with satisfactory results.

Keywords : Lamiophlomis rotata (Benth) Kudo, high-performance liquid chromatography, phenylethanoid glycosides, hydrolyzation,
Publishing house : University of Silesia in Katowice
Publication date : 2013
Number : Vol. 25, no. 2
Page : 377 – 392

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DOI :
Qute : Huo, H. L. ,Yu, S. H. ,Liu, X. Z. ,Meng, Y. ,Ren, Y. P. ,Zhang, L. T. ,Liu, L. ,Zhao, T. ,Cheng, X. M. ,Wang, C. H. ,Wang, Z. T. ,Gao, X. ,Jiang, X. ,Wang, L. ,Urban, J. ,Vaněk, J. ,Štys, D. ,Li, C. D. ,Liu, Y. ,Shi, Y. P. ,Zhang, Y. ,Peng, Q. ,Zeng, H. ,Yao, S. ,Zhang, Y. ,Song, H. ,Zhou, W. ,Zhang, X. Y. ,Lv, Y. P. ,Liu, X. D. ,Xu, C. ,Duan, G. L. ,Smoliński, A. ,Howaniec, N. ,Unver-Somer, N. ,Cicek-Polat, D. ,Emir, A. ,Onur, M. A. ,Kaya, G. I. ,Ahmed, A. B. A. ,Rao, A. S. ,Rao, M. V. ,Taha, R. M. ,Choi, Y. ,Shin, B. S. ,Kim, M. G. ,Shin, S. W. ,Kim, T. H. ,Jang, H. C. ,Yoo, S. D. ,Dong, S. ,Shi, X. ,Liu, Q. ,Zhang, Z. ,Zhao, L. ,Zhao, L. , Separation and analysis of phenylethanoid glycosides in Lamiophlomis rotata by high-performance liquid chromatography. Acta Chromatographica Vol. 25, no. 2/2013
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