Journal : Acta Chromatographica
Article : Simultaneous HPLC determination of isoniazid and acetylisoniazid in plasma

Authors :
Sherma, J.
Department of Chemistry, Lafayette College, Easton, PA 18042, USA,
Vrchotová, N.
Institute of Systems Biology and Ecology AS CR, Branišovská 31, 370 05 České Budějovice, Czech Republic,
Sivakumar, T.
Department of Pharmacy, Faculty of Engineering and Technology, Annamalai University, Annamalainagar, Tamil Nadu-608 002, India,
Petruczynik, A.
Department of Inorganic Chemistry, Medical University, Staszica 6, 20-081 Lublin, Poland,
Benkő, B.
PhD School of Semmelweis University, 1085 Budapest, Üllői út 26, Hungary, Division of Pharmacology and Drug Safety, Richter Gedeon Rt., Gyömrői út 21, 1475 Budapest, P.O. Box 27, Hungary, Department of Chemistry and Biochemistry, University of Pécs, Péc,
Mori, M.
Department of Chemistry, Faculty of Engineering, Gunma University, 1-5-1 Tenjin-cho, Kiryu, 376-8515, Japan,
Solangi, A. R.
National Center of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, Pakistan,
Idris, A. M.
Department of Chemistry, College of Science, King Faisal University, Hofuf 31982, Saudi Arabia,
Milán-Segovia, R.
Laboratorio de Farmacia, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Ave. Manuel Nava No. 6, 78290 San Luis Potosí, S.L.P., Mexico,
Abstract : A rapid and simple high-performance liquid chromatographic method has been developed for simultaneous determination of isoniazid (INH) and acetylisoniazid (AcINH) in microsamples of plasma. Plasma samples were deproteinated by addition of trichloroacetic acid, and the drug and its metabolite were then separated by reversed-phase HPLC on an octade-cylsilane-bonded silica column. The mobile phase was a gradient prepared from an aqueous solution of 1-hexanesulfonic acid sodium salt (pH 3) and acetonitrile. The effluent was monitored by UV detection at 290 nm. Cali-bration plots were linear in the range 0.5 to 15.0 µg mL-1 and the limit of quantification was 0.5 µg mL-1 for both drugs. The lower limits of detection for INH and AcINH were 0.24 and 0.12 µg mL-1, respectively. Results from analysis of quality-control samples at concentrations of 0.8, 5, and 13 µg mL-1 were indicative of good repeatability and precision. Recovery from plasma was 64% for INH and 55% for AcINH. INH was more stable than AcINH in plasma at –80°C.

Keywords :
Publishing house : University of Silesia in Katowice
Publication date : 2007
Number : No. 19
Page : 110 – 118

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Qute : Sherma, J. ,Vrchotová, N. ,Sivakumar, T. ,Petruczynik, A. ,Benkő, B. ,Mori, M. ,Solangi, A. R. ,Idris, A. M. ,Milán-Segovia, R. ,Milán-Segovia, R. , Simultaneous HPLC determination of isoniazid and acetylisoniazid in plasma. Acta Chromatographica No. 19/2007