Journal : Acta Chromatographica
Article : Simultaneous HPLC-DAD analysis of four methylated flavonols in Amomum koenigii

Authors :
Sherma, J.
Department of Chemistry, Lafayette College, Easton, PA 18042, USA,
Vrchotová, N.
Institute of Systems Biology and Ecology AS CR, Branišovská 31, 370 05 České Budějovice, Czech Republic,
Sivakumar, T.
Department of Pharmacy, Faculty of Engineering and Technology, Annamalai University, Annamalainagar, Tamil Nadu-608 002, India,
Petruczynik, A.
Department of Inorganic Chemistry, Medical University, Staszica 6, 20-081 Lublin, Poland,
Benkő, B.
PhD School of Semmelweis University, 1085 Budapest, Üllői út 26, Hungary, Division of Pharmacology and Drug Safety, Richter Gedeon Rt., Gyömrői út 21, 1475 Budapest, P.O. Box 27, Hungary, Department of Chemistry and Biochemistry, University of Pécs, Péc,
Mori, M.
Department of Chemistry, Faculty of Engineering, Gunma University, 1-5-1 Tenjin-cho, Kiryu, 376-8515, Japan,
Solangi, A. R.
National Center of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, Pakistan,
Idris, A. M.
Department of Chemistry, College of Science, King Faisal University, Hofuf 31982, Saudi Arabia,
Milán-Segovia, R.
Laboratorio de Farmacia, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Ave. Manuel Nava No. 6, 78290 San Luis Potosí, S.L.P., Mexico,
Qutab, S. S.
Schazoo Laboratories (Pvt) Ltd, 45 G.T. Road, Lahore-54000, Pakistan,
Agil, M.
Faculty of Pharmacy, Hamdard University New Delhi, 110 062, India,
Dhaneshwar, S. S.
Department of Pharmaceutical Chemistry, Poona College of Pharmacy, Bharati Vidyapeeth University, Erandwane, Pune-411 038, India,
Gannu, R.
Centre for Biopharmaceutics and Pharmacokinetics, University College of Pharmaceutical Sciences, Kakatiya University, Warangal, Andhra Pradesh, India,
Popović, G.
Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, P.O. Box 146, 11000 Belgrade, Serbia,
Mhaske, D. V.
Department of Quality Assurance Techniques and Pharmaceutical Chemistry, Bharati Vidyapeeth University, Poona College of Pharmacy, Centre for Advanced Pharmaceutical Research, Erandwane, Pune 411038, Maharashtra State, India,
Mohammad, A.
Analytical Research Laboratory, Department of Applied Chemistry, Faculty of Engineering and Technology, Aligarh Muslim University, Aligarh 202002, India,
Jain, G. K.
Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, New Delhi, 110062, India,
Dembitsky, V. M.
Department of Medicinal Chemistry and Natural Products, School of Pharmacy, P.O. Box 12065, The Hebrew University of Jerusalem, Jerusalem 91120, Israel,
Qiao, C. F.
Chinese Medicine Laboratory, Hong Kong Jockey Club Institute of Chinese Medicine, Hong Kong, China,
Abstract : Four bioactive methylated flavonols isolated from Amomum koenigii J.F. Gmelin, 3,7-dihydroxy-5,3',4'-trimethoxyflavone (1), 5-hydroxy-3,7,4'-trimethoxyflavone (2), 3,7-dihydroxy-5,4'-dimethoxyflavone (3), and 5-hydroxy-3,7,3',4'-tetramethoxyflavone (retusin, 4), have been analyzed by HPLC–DAD on a 4.6 mm × 150 mm, 5-µm particle, C8 column with an acetonitrile–aqueous acetic acid gradient as mobile phase. UV detection was at 360 nm. The method was validated in detail. The results indicated that the total amounts of these four constituents in the seeds were 0.776–0.829%, higher than in the pericarp. Retusin was the major constituent with a maximum content of approximately 0.50% in the seeds. This method is useful for quality control of the crude drug.

Keywords :
Publishing house : University of Silesia in Katowice
Publication date : 2007
Number : No. 19
Page : 217 – 226

: 1 A. Chevallier, Encyclopedia of Herbal Medicine, Dorling Kindersley Publishing, New York, 2000, pp 36–43
2 Z.Y. Wu and P.H. Raven, Flora of China, Vol. 24. Science Press, Beijing, 2000, pp 347-356
3 G.J. Xu, H.X. He, L.S. Xu, and R.L. Jin, Chinese Materia Medica, China Medico-Pharmaceutical Science and Technology, Beijing, 1996, pp 1193–1201
4 J.Y. Gao, Y.M. Xia, J.Y. Huang, and Q.J. Li, Zingiberaceae Flowers in China, Science Press, Beijing, 2006, pp 1–7
5 G.J. Xu and L.S. Xu, Species Systematization and Quality Evaluation of Commonly Used Chinese Traditional Drugs, Vol. 3, Fujian Science and Technology Publishing, Fuzhou, 1999, pp 398–414
6 H. Dong, Y.L. Gou, S.G. Cao, S.X. Chen, et al., Phytochemistry, 50, 899 (1999)
7 G.W. Zhang, X.Q. Ma, J.Y. Su, L.M. Zeng, et al., Chin. Tradit. Herb. Drug., 32, 870 (2001)
8 G. Citoglu, B. Sever, S. Antus, E. Baitz, and N. Altanlar, Pharm. Biol., 42, 659 (2004)
9 J.I. Murillo, R. Encarnacion, J. Malmstrom, C. Christophersen, and S.G. Franzblau, Fitoterapia, 74, 226 (2003)
10 R. Gonzalez, D.B. King, M.I. Aguilar, N. Diego, and H.B. Lotina, J. Agric. Food. Chem., 54, 1217 (2006)
11 J.F. Rivero, H.B. Chai, L.B. Kardono, F.M. Setyowati, et al., J. Nat. Prod., 67, 343 (2004)
12 M. Arisawa, T. Hayashi, M. Shimizu, N. Morita, et al., J. Nat. Prod., 54, 898 (1991)
13 M.H. Tchuendem, J.A. Mbah, A. Tsopmo, J.F. Ayafor, et al., Phytochemistry, 52, 1095 (1999)
14 A.S. Saratikov, V.S. Chuchalin, A.V. Ratkin, E.V. Ratkin, et al., Eksp. Klin. Farmakol., 68, 51 (2005)
15 Y. Yang, K. Kinoshita, K. Koyama, K. Takahashi, et al., Phytomedicine, 6, 89 (1999)
Qute : Sherma, J. ,Vrchotová, N. ,Sivakumar, T. ,Petruczynik, A. ,Benkő, B. ,Mori, M. ,Solangi, A. R. ,Idris, A. M. ,Milán-Segovia, R. ,Qutab, S. S. ,Agil, M. ,Dhaneshwar, S. S. ,Gannu, R. ,Popović, G. ,Mhaske, D. V. ,Mohammad, A. ,Jain, G. K. ,Dembitsky, V. M. ,Qiao, C. F. ,Qiao, C. F. , Simultaneous HPLC-DAD analysis of four methylated flavonols in Amomum koenigii. Acta Chromatographica No. 19/2007