Journal : Acta Chromatographica
Article : Simultaneous analysis of eprosartan and hydrochlorothiazide in tablets by high-performance thin-layer chromatography with ultraviolet absorption densitometry

Authors :
Dejaegher, B.
Vrije Universiteit Brussel — VUB Analytical Chemistry and Pharmaceutical Technology (FABI), Pharmaceutical Institute Laarbeeklaan 103 1090 Brussels Belgium,
Vander Heyden, Y.
Vrije Universiteit Brussel — VUB Analytical Chemistry and Pharmaceutical Technology (FABI), Pharmaceutical Institute Laarbeeklaan 103 1090 Brussels Belgium, yvanvdh@vub.ac.be,
Durón, R. R.
Universidad Autónoma de Nuevo León Departamento de Química Analítica, Facultad de Medicina P.O. Box 2316 Sucursal Tecnológico 64841 Monterrey Nuevo León México,
Almaguer, L. C.
Universidad Autónoma de Nuevo León Departamento de Química Analítica, Facultad de Medicina P.O. Box 2316 Sucursal Tecnológico 64841 Monterrey Nuevo León México,
Garza-Juárez, A.
Universidad Autónoma de Nuevo León Departamento de Química Analítica, Facultad de Medicina P.O. Box 2316 Sucursal Tecnológico 64841 Monterrey Nuevo León México,
Salazar Cavazos, M. L.
Universidad Autónoma de Nuevo León Departamento de Química Analítica, Facultad de Medicina P.O. Box 2316 Sucursal Tecnológico 64841 Monterrey Nuevo León México,
Waksman de Torres, N.
Universidad Autónoma de Nuevo León Departamento de Química Analítica, Facultad de Medicina P.O. Box 2316 Sucursal Tecnológico 64841 Monterrey Nuevo León México, nwaksman@fm.uanl.mx,
Garza-Juárez, A.
UANL, México Department of Analytical Chemistry, Facultad de Medicina Maderoy Aguirre Pequeño Col. Mitras Centro C.P. 64460 Mty, N.L. México,
Waksman de Torres, N.
UANL, México Department of Analytical Chemistry, Facultad de Medicina Maderoy Aguirre Pequeño Col. Mitras Centro C.P. 64460 Mty, N.L. México,
Salazar Cavazos, M. L.
UANL, México Department of Analytical Chemistry, Facultad de Medicina Maderoy Aguirre Pequeño Col. Mitras Centro C.P. 64460 Mty, N.L. México, masalaz52@yahoo.com,
Durón, R. R.
UANL, México Department of Analytical Chemistry, Facultad de Medicina Maderoy Aguirre Pequeño Col. Mitras Centro C.P. 64460 Mty, N.L. México,
Briciu, R. D.
Babeş-Bolyai University Faculty of Chemistry and Chemical Engineering Arany Janos Str. No 11 RO-400028 Cluj Napoca Romania,
Kot-Wasik, A.
Gdańsk University of Technology Chemical Faculty Narutowicza Str. No 11/12 80-952 Gdańsk Poland,
Namieśnik, J.
Gdańsk University of Technology Chemical Faculty Narutowicza Str. No 11/12 80-952 Gdańsk Poland,
Sârbu, C.
Babeş-Bolyai University Faculty of Chemistry and Chemical Engineering Arany Janos Str. No 11 RO-400028 Cluj Napoca Romania, costelsrb@yahoo.co.uk,
Sławik, T.
Medical University of Lublin Department of Medicinal Chemistry, Pharmaceutical Faculty Jaczewskiego 4 20-090 Lublin Poland,
Skibiński, R.
Medical University of Lublin Department of Medicinal Chemistry, Pharmaceutical Faculty Jaczewskiego 4 20-090 Lublin Poland, robert.skibinski@am.lublin.pl,
Paw, B.
Medical University of Lublin Department of Medicinal Chemistry, Pharmaceutical Faculty Jaczewskiego 4 20-090 Lublin Poland,
Działo, G.
Medical University of Lublin Department of Medicinal Chemistry, Pharmaceutical Faculty Jaczewskiego 4 20-090 Lublin Poland,
Sajewicz, M.
Silesian University Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Matlengiewicz, M.
Silesian University Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Kronenbach, D.
Silesian University Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Gontarska, M.
Silesian University Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Kowalska, T.
Silesian University Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland, kowalska@us.edu.pl,
Ekiert, R. J.
Jagiellonian University Department of Inorganic and Analytical Chemistry, Collegium Medicum 9 Medyczna St 30-688 Kraków Poland,
Krzek, J.
Jagiellonian University Department of Inorganic and Analytical Chemistry, Collegium Medicum 9 Medyczna St 30-688 Kraków Poland, jankrzek@cm-uj.krakow.pl,
Czekaj, J. S.
Jagiellonian University Department of Inorganic and Analytical Chemistry, Collegium Medicum 9 Medyczna St 30-688 Kraków Poland,
Hubicka, U.
Jagiellonian University Department of Inorganic and Analytical Chemistry, Collegium Medicum 9 Medyczna St 30-688 Kraków Poland,
Azeem, A.
Faculty of Pharmacy Department of Pharmaceutics Jamia Hamdard, New Delhi 110062 India,
Rizwan, M.
Faculty of Pharmacy Department of Pharmaceutics Jamia Hamdard, New Delhi 110062 India,
Ahmad, F. J.
Faculty of Pharmacy Department of Pharmaceutics Jamia Hamdard, New Delhi 110062 India,
Iqbal, Z.
Faculty of Pharmacy Department of Pharmaceutics Jamia Hamdard, New Delhi 110062 India,
Khar, R. K.
Faculty of Pharmacy Department of Pharmaceutics Jamia Hamdard, New Delhi 110062 India,
Aqil, M.
Faculty of Pharmacy Department of Pharmaceutics Jamia Hamdard, New Delhi 110062 India,
Talegaonkar, S.
Faculty of Pharmacy Department of Pharmaceutics Jamia Hamdard, New Delhi 110062 India, stalegaonkar@jamiahamdard.ac.in,
Bhusari, V. K.
Bharati Vidyapeeth University, Poona College of Pharmacy Department of Pharmaceutical Chemistry Pune Maharashtra India 411038,
Mahadik, M. V.
Bharati Vidyapeeth University, Poona College of Pharmacy Department of Pharmaceutical Chemistry Pune Maharashtra India 411038,
Dhaneshwar, S. R.
Bharati Vidyapeeth University, Poona College of Pharmacy Department of Pharmaceutical Chemistry Pune Maharashtra India 411038, sunil.dhaneshwar@gmail.com,
Patel, H. U.
Shri Sarvajanik Pharmacy College Department of Pharmaceutical Chemistry Near Arvind Baug Mehsana 384001 Gujarat India,
Suhagia, B. N.
L.M. College of Pharmacy Department of Pharmaceutical Chemistry Navrangpura, Ahmedabad 380009 Gujarat India,
Patel, C. N.
Shri Sarvajanik Pharmacy College Department of Pharmaceutical Chemistry Near Arvind Baug Mehsana 384001 Gujarat India, harshaupatel@yahoo.co.in,
Abstract : A new, simple, accurate, and precise high-performance thin-layer chromatographic (HPTLC) method has been established for simultaneous analysis of eprosartan and hydrochlorothiazide in tablet formulations. Standard and sample solutions of eprosartan and hydrochlorothiazide were applied to precoated silica gel G 60 F 254 HPTLC plates and the plates were developed with benzene-methanol-formic acid 7:3:0.1 ( υ/υ ) as mobile phase. Detection and evaluation of chromatograms was performed densitometrically at 272 nm. The retention factors of eprosartan and hydrochlorothiazide were 0.76 and 0.57, respectively. The linear range was 4.8–43.2 μg per spot for eprosartan and 0.15–1.35 μg per spot for hydrochlorothiazide; the correlation coefficients, r , were 0.998 and 0.999, respectively. The method was validated and successfully used for analysis of the drugs in tablets.

Keywords : HPTLC, eprosartan, hydrochlorothiazide, tablets, validation,
Publishing house : University of Silesia in Katowice
Publication date : 2009
Number : Vol. 21, no. 2
Page : 319 – 326

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DOI :
Qute : Dejaegher, B. ,Vander Heyden, Y. ,Durón, R. R. ,Almaguer, L. C. ,Garza-Juárez, A. ,Salazar Cavazos, M. L. ,Waksman de Torres, N. ,Garza-Juárez, A. ,Waksman de Torres, N. ,Salazar Cavazos, M. L. ,Durón, R. R. ,Briciu, R. D. ,Kot-Wasik, A. ,Namieśnik, J. ,Sârbu, C. ,Sławik, T. ,Skibiński, R. ,Paw, B. ,Działo, G. ,Sajewicz, M. ,Matlengiewicz, M. ,Kronenbach, D. ,Gontarska, M. ,Kowalska, T. ,Ekiert, R. J. ,Krzek, J. ,Czekaj, J. S. ,Hubicka, U. ,Azeem, A. ,Rizwan, M. ,Ahmad, F. J. ,Iqbal, Z. ,Khar, R. K. ,Aqil, M. ,Talegaonkar, S. ,Bhusari, V. K. ,Mahadik, M. V. ,Dhaneshwar, S. R. ,Patel, H. U. ,Suhagia, B. N. ,Patel, C. N. ,Patel, C. N. , Simultaneous analysis of eprosartan and hydrochlorothiazide in tablets by high-performance thin-layer chromatography with ultraviolet absorption densitometry. Acta Chromatographica Vol. 21, no. 2/2009
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