Journal : Acta Chromatographica
Article : Simultaneous analysis of losartan potassium, atenolol, and hydrochlorothiazide in bulk and in tablets by high-performance thin-layer chromatography with UV absorption densitometry

Authors :
Sherma, J.
Department of Chemistry, Lafayette College, Easton, PA 18042, USA,
Vrchotová, N.
Institute of Systems Biology and Ecology AS CR, Branišovská 31, 370 05 České Budějovice, Czech Republic,
Sivakumar, T.
Department of Pharmacy, Faculty of Engineering and Technology, Annamalai University, Annamalainagar, Tamil Nadu-608 002, India,
Petruczynik, A.
Department of Inorganic Chemistry, Medical University, Staszica 6, 20-081 Lublin, Poland,
Benkő, B.
PhD School of Semmelweis University, 1085 Budapest, Üllői út 26, Hungary, Division of Pharmacology and Drug Safety, Richter Gedeon Rt., Gyömrői út 21, 1475 Budapest, P.O. Box 27, Hungary, Department of Chemistry and Biochemistry, University of Pécs, Péc,
Mori, M.
Department of Chemistry, Faculty of Engineering, Gunma University, 1-5-1 Tenjin-cho, Kiryu, 376-8515, Japan,
Solangi, A. R.
National Center of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, Pakistan,
Idris, A. M.
Department of Chemistry, College of Science, King Faisal University, Hofuf 31982, Saudi Arabia,
Milán-Segovia, R.
Laboratorio de Farmacia, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Ave. Manuel Nava No. 6, 78290 San Luis Potosí, S.L.P., Mexico,
Qutab, S. S.
Schazoo Laboratories (Pvt) Ltd, 45 G.T. Road, Lahore-54000, Pakistan,
Agil, M.
Faculty of Pharmacy, Hamdard University New Delhi, 110 062, India,
Dhaneshwar, S. S.
Department of Pharmaceutical Chemistry, Poona College of Pharmacy, Bharati Vidyapeeth University, Erandwane, Pune-411 038, India,
Gannu, R.
Centre for Biopharmaceutics and Pharmacokinetics, University College of Pharmaceutical Sciences, Kakatiya University, Warangal, Andhra Pradesh, India,
Popović, G.
Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, P.O. Box 146, 11000 Belgrade, Serbia,
Mhaske, D. V.
Department of Quality Assurance Techniques and Pharmaceutical Chemistry, Bharati Vidyapeeth University, Poona College of Pharmacy, Centre for Advanced Pharmaceutical Research, Erandwane, Pune 411038, Maharashtra State, India,
Mohammad, A.
Analytical Research Laboratory, Department of Applied Chemistry, Faculty of Engineering and Technology, Aligarh Muslim University, Aligarh 202002, India,
Jain, G. K.
Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, New Delhi, 110062, India,
Dembitsky, V. M.
Department of Medicinal Chemistry and Natural Products, School of Pharmacy, P.O. Box 12065, The Hebrew University of Jerusalem, Jerusalem 91120, Israel,
Qiao, C. F.
Chinese Medicine Laboratory, Hong Kong Jockey Club Institute of Chinese Medicine, Hong Kong, China,
Waksmundzka-Hajnos, M.
Department of Inorganic Chemistry, Medical University of Lublin, Staszica 6, 20-081 Lublin, Poland,
Codreanu, M. V.
Department of Pharmaceutical Botany, “Carol Davila” University of Medicine and Pharmacy, 6 Traian Vuia Street, Bucharest, 70139, Romania,
Singh, D. P.
Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute, Lucknow, India,
Olszewska, M.
Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Łódź, 1 Muszyński Street, 90-151 Łódź, Poland,
Sathe, S. R.
Department of Pharmaceutical Chemistry, R.C. Patel College of Pharmacy, Karwand Naka, Shirpur 425 405, Dist-Dhule, (M.S.), India,
Abstract : A simple high-performance thin-layer chromatographic (HPTLC) method for separation and quantitative analysis of losartan potassium, atenolol, and hydrochlorothiazide in bulk and in pharmaceutical formulations has been established and validated. After extraction with methanol, sample and standard solutions were applied to prewashed silica gel plates and developed with toluene–methanol–triethylamine 6.5:4:0.5 (v/v) as mobile phase. Zones were scanned densitometrically at 274 nm. The RF values of losartan potassium, atenolol, and hydrochlorothiazide were 0.60, 0.43, and 0.29 respectively. Calibration plots were linear in the ranges 1000–5000 ng per band for losartan potassium and atenolol and 250–1250 ng per band for hydrochlorothiazide; the correlation coefficients, r, were 0.9994, 0.9993, and 0.9994, respectively. The suitability of this method for quantitative determination of these compounds was proved by validation in accordance with the requirements of pharmaceutical regulatory standards. The method was used for routine analysis of these drugs in bulk and in a formulation.

Keywords :
Publishing house : University of Silesia in Katowice
Publication date : 2007
Number : No. 19
Page : 270 – 278

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DOI :
Qute : Sherma, J. ,Vrchotová, N. ,Sivakumar, T. ,Petruczynik, A. ,Benkő, B. ,Mori, M. ,Solangi, A. R. ,Idris, A. M. ,Milán-Segovia, R. ,Qutab, S. S. ,Agil, M. ,Dhaneshwar, S. S. ,Gannu, R. ,Popović, G. ,Mhaske, D. V. ,Mohammad, A. ,Jain, G. K. ,Dembitsky, V. M. ,Qiao, C. F. ,Waksmundzka-Hajnos, M. ,Codreanu, M. V. ,Singh, D. P. ,Olszewska, M. ,Sathe, S. R. ,Sathe, S. R. , Simultaneous analysis of losartan potassium, atenolol, and hydrochlorothiazide in bulk and in tablets by high-performance thin-layer chromatography with UV absorption densitometry. Acta Chromatographica No. 19/2007
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