Simultaneous determination of amphetamine, methamphetamine, and caffeine in seized tablets by high-performance liquid chromatography

Czasopismo : Acta Chromatographica
Tytuł artykułu : Simultaneous determination of amphetamine, methamphetamine, and caffeine in seized tablets by high-performance liquid chromatography

Autorzy :
Bartnik, M.
Department of Pharmacognosy with Medicinal Plant Laboratory, Medical University, 1, Chodźki Str., 20-093 Lublin, Poland,
Kowalski, R.
Subdepartment of Food Quality Assessment and Central Apparatus Laboratory, Agricultural University, 20-950 Lublin, Akademicka 13, Poland,
Polak, B.
Department of Physical Chemistry, Faculty of Pharmacy, Medical University, Lublin, Poland,
Turło, J.
Department of Drug Technology, Medical University of Warsaw, 1, Banacha Street, 02-097 Warsaw, Poland,
Szyrwińska, K.
Department of Inorganic and Analytical Chemistry, Faculty of Pharmacy, Poznań University of Medical Sciences, Grunwaldzka 6, 60-780 Poznań, Poland,
Czauderna, M.
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland,
Bączek, T.
Medical University of Gdańsk, Department of Biopharmaceutics and Pharmacodynamics, Gdańsk, Poland,
Chilmonczyk, Z.
National Medicines Institute, 30/34, Chełmska Str., 00-725 Warsaw, Poland,
Hung, Ch. Y.
Department of Biotechnology, National Formosa University, Huwei, Yunlin, 632, Taiwan,
Baboota, S.
Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, Hamdard Nagar, New Delhi-110062, India,
Sivakumar, T.
Department of Pharmacy, Annamalai University, Annamalai Nagar, Tamil Nadu-608 002, India,
Ivanović, D.
Faculty of Pharmacy, Department of Drug Analysis, Vojvode Stepe 450, 11000 Belgrade, Serbia,
Pavlova, V.
Institute of Chemistry, Faculty of Natural Sciences and Mathematics, Saints Cyril and Methodius University, Arhimedova 5, P.O. Box 162, 1000 Skopje, Macedonia,
Abstrakty : A new, inexpensive HPLC method has been developed for determination of amphetamine and methamphetamine in combination with caffeine, the most widely used adulterant in seized tablets. Reversed-phase chromatography was performed at 40°C with 90:10 (v/v) aqueous orthophosphoric acid (pH 2.1)–acetonitrile as mobile phase at a flow rate of 1.5 mL min-1. All the analytes were quantified at 205 nm. Statistical validation of the method included determination of selectivity, linearity, accuracy, and precision. The method is rapid, simple, and reproducible and could be used for direct determination of amphetamine, methamphetamine, and caffeine in seized tablets. The identity of each compound in real samples was established by comparing retention times and UV spectra with those of standards.

Słowa kluczowe :
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2007
Numer : No. 18
Strony : 157 – 167
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Cytuj : Bartnik, M. ,Kowalski, R. ,Polak, B. ,Turło, J. ,Szyrwińska, K. ,Czauderna, M. ,Bączek, T. ,Chilmonczyk, Z. ,Hung, Ch. Y. ,Baboota, S. ,Sivakumar, T. ,Ivanović, D. ,Pavlova, V. , Simultaneous determination of amphetamine, methamphetamine, and caffeine in seized tablets by high-performance liquid chromatography. Acta Chromatographica No. 18/2007