Journal : Acta Chromatographica
Article : Simultaneous determination of celiprolol HCl and chlorthalidone in tablets and biological fluids using high-performance liquid chromatography

Authors :
Sajewicz, M.
University of Silesia Department of General Chemistry and Chromatography 9 Szkolna Street 40-006 Katowice Poland,
Kowalska, T.
University of Silesia Department of General Chemistry and Chromatography 9 Szkolna Street 40-006 Katowice Poland, teresa.kowalska@us.edu.pl,
Szalka, M.
ICN Polfa Rzeszów S.A. Product Development Department 35-959 Rzeszów Poland,
Kostka, J.
Rzeszów University of Technology Department of Chemical and Process Engineering 35-959 Rzeszów,
Rokaszewski, E.
ICN Polfa Rzeszów S.A. Product Development Department 35-959 Rzeszów Poland,
Kaczmarski, K.
Rzeszów University of Technology Department of Chemical and Process Engineering 35-959 Rzeszów, kkaczmarski@prz.edu.pl,
Belal, F.
University of Mansoura Department of Analytical Chemistry, Faculty of Pharmacy 35516 Mansoura Egypt,
El-Brashy, A. M.
University of Mansoura Department of Analytical Chemistry, Faculty of Pharmacy 35516 Mansoura Egypt,
El-Enany, N.
University of Mansoura Department of Analytical Chemistry, Faculty of Pharmacy 35516 Mansoura Egypt,
Tolba, M. M.
University of Mansoura Department of Analytical Chemistry, Faculty of Pharmacy 35516 Mansoura Egypt,
Abstract : A simple, accurate, and sensitive reversed-phase HPLC method was developed for the simultaneous determination of celiprolol HCl (CE) and chlorthalidone (CT). Good chromatographic separation was achieved using a 250 mm × 4.6 mm i.d., 5 μm particle size Hypurity C8 column. Mobile phase containing a mixture of methanol and 0.04 M phosphate buffer (35:65, υ/υ) at pH 7.0 was pumped at a flow rate of 1.2 mL min-1with UV detection at 225 nm. Lisinopril dihydrate (LIS) was used as internal standard. The method showed good linearity in the ranges 0.2–20 and 0.2–10 μg mL-1with limits of detection 0.06 and 0.04 μg mL-1and limits of quantification 0.20 and 0.14μg mL-1for CE and CT, respectively. The suggested method was successfully applied to the simultaneous analysis of the studied drugs in their synthetic mixture and coformulated tablet. The method was further extended to the determination of CE in biological fluids. The proposed method was also applied to the determination of the studied drugs in presence of some coadministered or related drugs such as atenolol, propranolol, acetazolamide, enalapril, nicardipine, triamterene, and hydrochlorothiazide without any interference.

Keywords : HPLC, celiprolol HCl (CE), chlorthalidone (CT), coformulated tablets, biological fluids,
Publishing house : University of Silesia in Katowice
Publication date : 2012
Number : Vol. 24, no. 2
Page : 185 – 206

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DOI :
Qute : Sajewicz, M. ,Kowalska, T. ,Szalka, M. ,Kostka, J. ,Rokaszewski, E. ,Kaczmarski, K. ,Belal, F. ,El-Brashy, A. M. ,El-Enany, N. ,Tolba, M. M. ,Tolba, M. M. , Simultaneous determination of celiprolol HCl and chlorthalidone in tablets and biological fluids using high-performance liquid chromatography. Acta Chromatographica Vol. 24, no. 2/2012
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