Journal : Acta Chromatographica
Article : Simultaneous determination of ephedra alkaloids in traditional chinese medicines by high-performance liquid chromatography

Authors :
Bhushan, R.
Indian Institute of Technology Roorkee Department of Chemistry Roorkee 247667 India, rbushfcy@iitr.ernet.in,
Dixit, S.
Indian Institute of Technology Roorkee Department of Chemistry Roorkee 247667 India,
Mátyus, M.
Hungarian Defence Forces Dr György Radó Military Medical Center Budapest Hungary,
Kocsis, G.
Hungarian Defence Forces Dr György Radó Military Medical Center Budapest Hungary,
Boldis, O.
Hungarian Defence Forces Dr György Radó Military Medical Center Budapest Hungary,
Karvaly, G.
Scientific Institute Military Hospital-State Health Centre P.O. Box 1 H-1553 Budapest Hungary,
Magyari, E.
Hungarian Defence Forces Dr György Radó Military Medical Center Budapest Hungary,
Fűrész, J.
Scientific Institute Military Hospital-State Health Centre P.O. Box 1 H-1553 Budapest Hungary,
Gachályi, A.
Hungarian Defence Forces Dr György Radó Military Medical Center Budapest Hungary,
Mantovani, L.
Universidade Federal do Pampa Laboratório de Pesquisa e Desenvolvimento em Controle de Qualidade de Medicamentos BR 472 km 585 Prédio Administrativo CEP 97500-970 Uruguaiana-RS Brazil,
Sayago, C. T. M.
Universidade Federal do Pampa Laboratório de Pesquisa e Desenvolvimento em Controle de Qualidade de Medicamentos BR 472 km 585 Prédio Administrativo CEP 97500-970 Uruguaiana-RS Brazil,
Camargo, V. B.
Universidade Federal do Pampa Laboratório de Pesquisa e Desenvolvimento em Controle de Qualidade de Medicamentos BR 472 km 585 Prédio Administrativo CEP 97500-970 Uruguaiana-RS Brazil,
Silveira, V. F.
Universidade Federal do Pampa Laboratório de Pesquisa e Desenvolvimento em Controle de Qualidade de Medicamentos BR 472 km 585 Prédio Administrativo CEP 97500-970 Uruguaiana-RS Brazil,
Garcia, C. V.
Universidade Federal do Rio Grande do Sul Programa de Pós-graduação em Ciências Farmacêuticas Av. Ipiranga 2752 Lab 402 CEP 90610-000 Porto Alegre-RS Brazil,
Schapoval, E. E. S.
Universidade Federal do Rio Grande do Sul Programa de Pós-graduação em Ciências Farmacêuticas Av. Ipiranga 2752 Lab 402 CEP 90610-000 Porto Alegre-RS Brazil,
Mendez, A. S. L.
Universidade Federal do Pampa Laboratório de Pesquisa e Desenvolvimento em Controle de Qualidade de Medicamentos BR 472 km 585 Prédio Administrativo CEP 97500-970 Uruguaiana-RS Brazil, andreaslmendez@yahoo.com.br,
Cao, L.-W.
Jinan University Department of Chemistry Guangzhou 510632 People’s Republic of China, lwcao@126.com,
Li, C.
Jinan University Department of Chemistry Guangzhou 510632 People’s Republic of China,
Desai, D.
The Maharaja Sayajirao University of Baroda Quality Assurance Laboratory, Centre of Relevance and Excellence in Novel Drug Delivery System, Pharmacy Department, G. H. Patel Pharmacy Building, Donor’s Plaza Fatehgunj, Vadodara 390 002 Gujarat India,
Patel, G.
The Maharaja Sayajirao University of Baroda Quality Assurance Laboratory, Centre of Relevance and Excellence in Novel Drug Delivery System, Pharmacy Department, G. H. Patel Pharmacy Building, Donor’s Plaza Fatehgunj, Vadodara 390 002 Gujarat India,
Shukla, N.
The Maharaja Sayajirao University of Baroda Quality Assurance Laboratory, Centre of Relevance and Excellence in Novel Drug Delivery System, Pharmacy Department, G. H. Patel Pharmacy Building, Donor’s Plaza Fatehgunj, Vadodara 390 002 Gujarat India,
Rajput, S.
The Maharaja Sayajirao University of Baroda Quality Assurance Laboratory, Centre of Relevance and Excellence in Novel Drug Delivery System, Pharmacy Department, G. H. Patel Pharmacy Building, Donor’s Plaza Fatehgunj, Vadodara 390 002 Gujarat India, sjrajput@gmail.com,
Joshi, S. S.
National Institute of Pharmaceutical Education and Research (NIPER) Balanagar, Hyderabad 500037 India,
Nahire, R. R.
National Institute of Pharmaceutical Education and Research (NIPER) Balanagar, Hyderabad 500037 India,
Shastri, N. R.
National Institute of Pharmaceutical Education and Research (NIPER) Balanagar, Hyderabad 500037 India, svcphod@yahoo.co.in,
Surendranath, K. V.
ICICI Knowledge Park United States Pharmacopeia India Private Limited, Reference Standard Laboratory Shameerpet, Hyderabad 500078 India,
Satish, J.
ICICI Knowledge Park United States Pharmacopeia India Private Limited, Reference Standard Laboratory Shameerpet, Hyderabad 500078 India,
Kobyłka, M.
Medical University of Lublin Department of Medicinal Chemistry Jaczewskiego 4 20-090 Lublin Poland,
Komsta, Ł.
Medical University of Lublin Department of Medicinal Chemistry Jaczewskiego 4 20-090 Lublin Poland, lukasz.komsta@umlub.pl,
Adegoke, O. A.
University of Ibadan Department of Pharmaceutical Chemistry, Faculty of Pharmacy Ibadan Nigeria, ao.adegoke@mail.ui.edu.ng,
Xiang, L. L.
Chinese Academy of Sciences Laboratory of Pharmaceutical Analysis, Shanghai Institute of Materia Medica 555 Zuchongzhi Road Shanghai 201203 PR China,
Idowu, O. S.
University of Ibadan Department of Pharmaceutical Chemistry, Faculty of Pharmacy Ibadan Nigeria,
Chen, D.-Y.
Chinese Academy of Sciences Laboratory of Pharmaceutical Analysis, Shanghai Institute of Materia Medica 555 Zuchongzhi Road Shanghai 201203 PR China, dychen@mail.shcnc.ac.cn,
Omar, A. A.
Alexandria University Department of Pharmacognosy, Faculty of Pharmacy Alexandria 21521 Egypt,
Hadad, G. M.
Suez Canal University Department of Analytical Chemistry, Faculty of Pharmacy Ismailia 41522 Egypt,
Badr, J. M.
King Abdulaziz University Department of Natural Products and Alternative Medicine, Faculty of Pharmacy Jeddah 21589 Kingdom of Saudi Arabia, jihanbadr2010@hotmail.com,
Chen, Y.
Southern Medical University Key Laboratory of Chinese Drugs Pharmaceutics of Guang Dong Province, School of Traditional Chinese Medicine 510515 Guangzhou People’s Republic of China,
Li, X.
Chifeng Aike Pharmaceutical Technology Co., Ltd. 024001 Chifeng People’s Republic of China,
Chen, F.
Southern Medical University Key Laboratory of Chinese Drugs Pharmaceutics of Guang Dong Province, School of Traditional Chinese Medicine 510515 Guangzhou People’s Republic of China,
Zhu, Q.
Southern Medical University Key Laboratory of Chinese Drugs Pharmaceutics of Guang Dong Province, School of Traditional Chinese Medicine 510515 Guangzhou People’s Republic of China,
Abstract : A rapid, simple, and practical high-performance liquid chromatographic method (HPLC) was developed and validated for the simultaneous determination of norephedrine (NME), norpseudoephedrine (NMP), ephedrine (E), pseudoephedrine (PE), and methylephedrine (ME) in traditional Chinese medicines (TCM) which contained Ephedrae Herba (Ephedra). This analysis could be accomplished within 12.5 min with an Alltima Phenyl Column by isocratic elution using a mixture of KH2PO4 (20 mM)-acetonitrile (96:4, v/v) as the mobile phase at a flow-rate of 0.6 mL min−1 and a wavelength of 210 nm. This method was successfully applied to quantify ephedra alkaloids in both Ma-xing-gan-shi decoction and Ephedra decoction. The concentration of total ephedra alkaloids (4.62 mg mL−1) in Ma-xing-gan-shi decoction was much lower than that (7.10 mg mL−1) in Ephedra decoction. Furthermore, the concentration of NME, NMP, E, PE, and ME was significantly lower in Ma-xing-gan-shi decoction than that in Ephedra decoction, respectively. The method was easily acceptable and would be popular with most analytical laboratories.

Keywords : traditional Chinese medicine, ephedra alkaloids, Ephedra decoction, Ma-xing-gan-shi decoction, HPLC-UV,
Publishing house : University of Silesia in Katowice
Publication date : 2012
Number : Vol. 24, no. 3
Page : 475 – 487

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DOI :
Qute : Bhushan, R. ,Dixit, S. ,Mátyus, M. ,Kocsis, G. ,Boldis, O. ,Karvaly, G. ,Magyari, E. ,Fűrész, J. ,Gachályi, A. ,Mantovani, L. ,Sayago, C. T. M. ,Camargo, V. B. ,Silveira, V. F. ,Garcia, C. V. ,Schapoval, E. E. S. ,Mendez, A. S. L. ,Cao, L.-W. ,Li, C. ,Desai, D. ,Patel, G. ,Shukla, N. ,Rajput, S. ,Joshi, S. S. ,Nahire, R. R. ,Shastri, N. R. ,Surendranath, K. V. ,Satish, J. ,Kobyłka, M. ,Komsta, Ł. ,Adegoke, O. A. ,Xiang, L. L. ,Idowu, O. S. ,Chen, D.-Y. ,Omar, A. A. ,Hadad, G. M. ,Badr, J. M. ,Chen, Y. ,Li, X. ,Chen, F. ,Zhu, Q. ,Zhu, Q. , Simultaneous determination of ephedra alkaloids in traditional chinese medicines by high-performance liquid chromatography. Acta Chromatographica Vol. 24, no. 3/2012
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