Simultaneous determination of fusidic acid and steroids from bulk drugs and human plasma by reversed phase HPLC

Czasopismo : Acta Chromatographica
Tytuł artykułu : Simultaneous determination of fusidic acid and steroids from bulk drugs and human plasma by reversed phase HPLC

Autorzy :
Komsta, Ł.
Medical University of Lublin Chair and Department of Medicinal Chemistry, Faculty of Pharmacy Jaczewskiego 4 20-090 Lublin Poland, lukasz.komsta@am.lublin.pl,
Gowin, E.
Medical University of Lublin Chair and Department of Medicinal Chemistry, Faculty of Pharmacy Jaczewskiego 4 20-090 Lublin Poland,
Djordjević Filijović, N.
Hemofarm AD Vrsac Serbia,
Pavlović, A.
Hemofarm AD Vrsac Serbia,
Nikolić, K.
University of Belgrade, Faculty of Pharmacy Department of Pharmaceutical Chemistry Vojvode Stepe 450 PO Box 146 11000 Belgrade Serbia,
Agbaba, D.
University of Belgrade, Faculty of Pharmacy Department of Pharmaceutical Chemistry Vojvode Stepe 450 PO Box 146 11000 Belgrade Serbia, danica@pharmacy.bg.ac.rs,
Sawant, S.
Sinhgad Technical Education Society’s Smt. Kashibai Navale College of Pharmacy, Kondhwa (Bk.) Pune 411 048 Maharashtra India, sawant99@rediffmail.com,
Barge, V.
PDEA’s Seth Govind Raghunath Sable College of Pharmacy Saswad, Tal-Purandar, Dist-Pune 412301 Maharashtra India,
Tiwari, R.
School of Pharmacy and Technology Department of Pharmaceutical Chemistry, SVKM’s NMIMS Management Shirpur Dist. Dhule 425405 Maharashtra India, ravisun4@rediffmail.com,
Bonde, C.
School of Pharmacy and Technology Department of Pharmaceutical Chemistry, SVKM’s NMIMS Management Shirpur Dist. Dhule 425405 Maharashtra India,
Bothara, K.
Sinhgad Institute of Pharmacy Department of Pharmaceutical Chemistry Narhe Road Pune 410041 Maharashtra India,
Nawaz, M.
United Arab Emirates University Department of Chemistry Al-Ain 17551 UAE, nawwaz@gmail.com,
Arayne, M. S.
University of Karachi Department of Chemistry Karachi 75270 Pakistan,
Sultana, N.
University of Karachi Research Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, Faculty of Pharmacy Karachi 75270 Pakistan,
Haider, A.
University of Karachi Department of Chemistry Karachi 75270 Pakistan,
Hisaindee, S.
United Arab Emirates University Department of Chemistry Al-Ain 17551 UAE,
Abstrakty : A simple, rapid, and sensitive high-performance liquid chromatography method was developed and validated for the simultaneous determination and quantification of fusidic acid and steroids (prednisone, betamethasone valerate, hydrocortisone acetate, and dexamethasone sodium) from bulk drugs and human plasma. A RP-HPLC, operated at ambient temperature, was equipped with a UV detector for monitoring the effluents at 235 nm. The mobile phase consisted of methanol, acetonitrile, and 0.05 M phosphoric acid (10:60:30, v/v/v), and separation was achieved on a Medeterrane, C18 (5 μm, 12.5 × 0.46 mm) column at a flow rate of 1.7 mL min-1. Calibration curves were linear over concentration range 0.625–10 μg mL-1with correlation coefficient (r2) greater than 0.9999. The coefficient of variation (CV) and relative error (RE) for intra- and interassay were <2% and <1%, respectively. Interference of other already administered common medicaments, such as aspirin, paracetamol, caffeine, nicotine, and other plasma components, were not found.
Słowa kluczowe : fusidic acid, steroids, HPLC,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2014
Numer : Vol. 26, no. 1
Strony : 57 – 66
Bibliografia : 1 J.D. Wilkinson. Br J Dermatol., 139, 37 (1998)
2 I. Elsaku, Clin. Chem., 33, 1412 (1987)
3 S.C. Siegel. Clin Rev Allerg., 1, 123–146 (1983)
4 P.L. Toutain. J Chromatogr., 309, 385 (1984)
5 D. S. C. Hui, and J. J. Y. Sung, Chest, 124, 12 (2003)
6 D. S. C. Hui, and J. J. Y. Sung, Chest, 126, 670 (2004)
7 S. T. Lai, Eur. J. Clin. Microbiol. Infect. Dis., 24, 583 (2005)
8 J.Q. Rose, and W.J. Juscko, J. Chromatogr., 162, 273 (1979)
9 M.C. Petersen, R.L. Nation, and J.J. Ashley, J Chromatogr., 183, 131 (1980)
10 I.T. Agabeyoglu, J.G. Wagner, and D.R. Kay, Res. Commun. Chem. Pathol. Pharmacol., 28, 163 (1980)
11 L. Oat, Falk, O. Lantto, and O. Bjorkhem. Scand J. Clin. Lab. Invest., 42, 181 (1982)
12 J. Korpi, D.P. Wittmer, B.J. Sandmann, and W.G. Haney. Jr. J. Pharm. Sci., 65, 1087 (1976)
13 M.D. Smith, J. Chromatogr., 164, 129 (1979)
14 M.S. Arayne, N. Sultana, and M. Nawaz, J. Anal. Chem., 63, 881–887 (2008)
15 The European Agency for the Evaluation of Medical Products. ICH TopicQ2B Note for Guidance on Validation of Analytical Procedures: Methodology GPMP/ICH/281/95, 1996.
DOI :
Cytuj : Komsta, Ł. ,Gowin, E. ,Djordjević Filijović, N. ,Pavlović, A. ,Nikolić, K. ,Agbaba, D. ,Sawant, S. ,Barge, V. ,Tiwari, R. ,Bonde, C. ,Bothara, K. ,Nawaz, M. ,Arayne, M. S. ,Sultana, N. ,Haider, A. ,Hisaindee, S. , Simultaneous determination of fusidic acid and steroids from bulk drugs and human plasma by reversed phase HPLC. Acta Chromatographica Vol. 26, no. 1/2014
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