Journal : Acta Chromatographica
Article : Simultaneous determination of loratadine and preservatives in syrups by thin-layer chromatography

Authors :
Sherma, J.
Department of Chemistry, Lafayette College, Easton, PA 18042, USA,
Vrchotová, N.
Institute of Systems Biology and Ecology AS CR, Branišovská 31, 370 05 České Budějovice, Czech Republic,
Sivakumar, T.
Department of Pharmacy, Faculty of Engineering and Technology, Annamalai University, Annamalainagar, Tamil Nadu-608 002, India,
Petruczynik, A.
Department of Inorganic Chemistry, Medical University, Staszica 6, 20-081 Lublin, Poland,
Benkő, B.
PhD School of Semmelweis University, 1085 Budapest, Üllői út 26, Hungary, Division of Pharmacology and Drug Safety, Richter Gedeon Rt., Gyömrői út 21, 1475 Budapest, P.O. Box 27, Hungary, Department of Chemistry and Biochemistry, University of Pécs, Péc,
Mori, M.
Department of Chemistry, Faculty of Engineering, Gunma University, 1-5-1 Tenjin-cho, Kiryu, 376-8515, Japan,
Solangi, A. R.
National Center of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, Pakistan,
Idris, A. M.
Department of Chemistry, College of Science, King Faisal University, Hofuf 31982, Saudi Arabia,
Milán-Segovia, R.
Laboratorio de Farmacia, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Ave. Manuel Nava No. 6, 78290 San Luis Potosí, S.L.P., Mexico,
Qutab, S. S.
Schazoo Laboratories (Pvt) Ltd, 45 G.T. Road, Lahore-54000, Pakistan,
Agil, M.
Faculty of Pharmacy, Hamdard University New Delhi, 110 062, India,
Dhaneshwar, S. S.
Department of Pharmaceutical Chemistry, Poona College of Pharmacy, Bharati Vidyapeeth University, Erandwane, Pune-411 038, India,
Gannu, R.
Centre for Biopharmaceutics and Pharmacokinetics, University College of Pharmaceutical Sciences, Kakatiya University, Warangal, Andhra Pradesh, India,
Popović, G.
Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, P.O. Box 146, 11000 Belgrade, Serbia,
Abstract : Simple, rapid, and precise densitometric TLC methods have been established for simultaneous determination of loratadine and preservatives in loratadine–sodium benzoate and loratadine–methylparaben–propylparaben mixtures. Chromatography was performed on aluminium plates precoated with silica gel 60 F254. The mobile phases were n-butyl acetate–carbon tetrachloride–acetic acid–acetonitrile 3:6:0.2:3 (v/v) for loratadine–sodium benzoate mixtures and ethyl acetate–n-hexane–methanol–ammonia–diethylamine 1:4:0.8:0.4:2 (v/v) for loratadine–methylparaben–propylparaben mixtures. Chromatographic zones were scanned in reflectance–absorbance mode at 240 nm for the former mixture and 275 nm for the latter. Relationships between peak areas and amounts of the compounds were evaluated by linear regression analysis in the concentration ranges 0.3–0.7 µg per band for loratadine, methylparaben, and sodium benzoate and 0.03–0.07 µg per band for propylparaben. Mean recovery for all the compounds varied from 98.3 to 102.5 %. Detection and quantification limits ranged from 0.004 to 0.03 and from 0.01 to 0.1 µg per band, respectively. The proposed method was used for simultaneous determination of loratadine and the preservatives in commercial medicinal syrups.

Keywords :
Publishing house : University of Silesia in Katowice
Publication date : 2007
Number : No. 19
Page : 161 – 169

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Qute : Sherma, J. ,Vrchotová, N. ,Sivakumar, T. ,Petruczynik, A. ,Benkő, B. ,Mori, M. ,Solangi, A. R. ,Idris, A. M. ,Milán-Segovia, R. ,Qutab, S. S. ,Agil, M. ,Dhaneshwar, S. S. ,Gannu, R. ,Popović, G. ,Popović, G. , Simultaneous determination of loratadine and preservatives in syrups by thin-layer chromatography. Acta Chromatographica No. 19/2007