Journal : Acta Chromatographica
Article : Simultaneous determination of lycorine and galanthamine in Galanthus woronowii by HPLC-DAD

Authors :
Patil, A. N.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India,
Nirmal, S. A.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India, nirmalsunil@rediffmail.com,
Chavan, A. K.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India,
Zhou, L.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Xu, J.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Luan, L.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Ma, J.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Gong, Y.
Ministry of Agriculture Institute for the Control of Agrochemicals Beijing 100026 China,
Qin, D.
Ministry of Agriculture Institute for the Control of Agrochemicals Beijing 100026 China,
Pan, C.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Jinlong, M.
Zhejiang University Department of Chemical and Biological Engineering Hangzhou 310027 China,
Guobin, J.
Dalian Nationalities University Environment and Resources College Dalian 11660 China,
Bo, J.
Dalian Nationalities University Life Science College Dalian 116600 China,
Hua, J.
Zhejiang University Department of Chemical and Biological Engineering Hangzhou 310027 China,
Montejo, C.
Universidad CEU San Pablo Departamento de Ciencias Farmacéuticas y de la Alimentación, Área de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28668 Madrid Spain, montejo@ceu.es,
Civera, C.
Universidad Complutense de Madrid Departamento de Química Física II, Facultad de Farmacia 28040 Madrid Spain,
Barcia, E.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Negro, S.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Fernández-Carballido, A.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Czauderna, M.
Polish Academy of Sciences The Kielanowski Institute of Animal Physiology and Nutrition 05-110 Jabłonna Poland, mr.czauderna@gmail.com,
Marounek, M.
Prague-Uhříněves The Institute of Animal Science Prague Czech Republic,
Duskova, D.
Prague-Uhříněves The Institute of Animal Science Prague Czech Republic,
Kowalczyk, J.
Polish Academy of Sciences The Kielanowski Institute of Animal Physiology and Nutrition 05-110 Jabłonna Poland,
Konda, R. K.
Hindu College of Pharmacy Department of Pharmaceutical Chemistry Amaravathi Road Guntur Andhrapradesh 522002 India, victory2ravi@yahoo.co.in,
Chandu, B. R.
Donbosco College of Pharmacy Department of Pharmaceutical Sciences Pulladigunta Guntur 522201 India,
Challa, B. R.
Nirmala College of Pharmacy Department of Pharmaceutical Analysis Madras Road Kadapa Andhrapradesh 516002 India, baluchalla_99@yahoo.com,
Chandrasekhar, K. B.
Jawaharlal Nehru Technological University Department of Chemistry Anantapur 515002 India,
Shen, Y.
Jiangsu University School of Pharmacy Zhenjiang China,
Yang, H.
Jiangsu University School of Pharmacy Zhenjiang China,
Xia, G.
Jiangsu University School of Pharmacy Zhenjiang China,
Wang, J.
Jiangsu University School of Pharmacy Zhenjiang China,
Cai, B.
Nanjing University of Chinese Medicine Jiangsu Key Laboratory of Chinese Medicine Processing, SATCM Key Laboratory of Standardization for Chinese Medicine Processing Nanjing China,
Jia, X.
Jiangsu University School of Pharmacy Zhenjiang China, jxiaobin2005@hotmail.com,
Nurulain, S. M.
United Arab Emirates University Department of Pharmacology and Pharmacotherapy Al Ain United Arab Emirates,
Kalász, H.
Semmelweis University Department of Pharmacology and Pharmacotherapy Budapest Hungary, huba.kalasz@gmail.com,
Szegi, P.
Semmelweis University Department of Pharmacodynamics Budapest Hungary,
Kuca, K.
University Hospital Biomedical Research Center Hradec Kralove Czech Republic,
Adem, A.
United Arab Emirates University Department of Pharmacology and Pharmacotherapy Al Ain United Arab Emirates,
Hasan, M. Y. B.
United Arab Emirates University Department of Pharmacology and Pharmacotherapy Al Ain United Arab Emirates,
Hashemi, F.
Semmelweis University Department of Pharmacodynamics Budapest Hungary,
Tekes, K.
Semmelweis University Department of Pharmacodynamics Budapest Hungary,
Sangoi, M. S.
Federal University of Rio Grande do Sul Faculty of Pharmacy 90610-000 Porto Alegre-RS Brazil, maxsilvasangoi@yahoo.com.br,
Wrasse-Sangoi, M.
Federal University of Santa Maria Department of Industrial Pharmacy 97105-900 Santa Maria-RS Brazil,
Hurtado, F. K.
Federal University of Rio Grande do Sul Faculty of Pharmacy 90610-000 Porto Alegre-RS Brazil,
Rolim, C. M. B.
Federal University of Santa Maria Department of Industrial Pharmacy 97105-900 Santa Maria-RS Brazil,
Walorczyk, S.
Institute of Plant Protection — National Research Institute Department of Pesticide Residue Research Władysława Węgorka 20 60-318 Poznań Poland, s_walorczyk@tlen.pl,
Li, W.
Jilin Agricultural University College of Chinese Medicinal Material Changchun 130118 China,
Shi, L. L.
Jilin Agricultural University College of Chinese Medicinal Material Changchun 130118 China,
Han, L. Q.
Jilin Agricultural University College of Chinese Medicinal Material Changchun 130118 China,
Zhang, J.
Jilin Agricultural University College of Chinese Medicinal Material Changchun 130118 China, zhjing0701@163.com,
Mirjalili, M. H.
Shahid Beheshti University Medicinal Plants and Drugs Research Institute G.C., Evin Tehran Iran,
Navarro, A.
National Autonomous University of Mexico Department of Food and Biotechnology, Faculty of Chemistry Ciudad de México 04510 Mexico,
Hernández, L.
Universidad Autónoma Metropolitana-Xochimilco Departamento de Sistemas Biológicos Calz. del Hueso 1100, Col. Villa Quietud 04960 México, D. F. México,
Jáuregui, O.
Universitat de Barcelona Scientific & Technological Centers (CCiTUB) c/Baldiri i Reixach 10-12 08028 Barcelona Spain,
Bonfill, M.
Universitat de Barcelona Laboratori de Fisiologia Vegetal, Facultat de Farmàcia Avda. Joan XXIII s/n 08028 Barcelona Spain, mbonfill@ub.edu,
Emir, A.
Ege University Department of Pharmacognosy, Faculty of Pharmacy 35100 Bornova, Izmir Turke,
Cicek-Polat, D.
Ege University Department of Pharmacognosy, Faculty of Pharmacy 35100 Bornova, Izmir Turke,
Kaya, G. I.
Ege University Department of Pharmacognosy, Faculty of Pharmacy 35100 Bornova, Izmir Turke,
Sarikaya, B.
Ege University Department of Pharmacognosy, Faculty of Pharmacy 35100 Bornova, Izmir Turke,
Onur, M. A.
Ege University Department of Pharmacognosy, Faculty of Pharmacy 35100 Bornova, Izmir Turke,
Unver-Somer, N.
Ege University Department of Pharmacognosy, Faculty of Pharmacy 35100 Bornova, Izmir Turke,
Abstract : Galanthamine, used as a drug for the treatment of Alzheimer’s disease and lycorine possessing important biological properties, are widely distributed alkaloids in the members of Amaryllidaceae family. In the present study, the contents of these alkaloids have been quantitatively determined by high-performance liquid chromatography (HPLC) coupled with diode array detector (DAD) in the aerial parts and bulbs of Galanthus woronowii Losinsk. collected from different localities in Northeastern Turkey. A simple extraction method utilizing columns pre-packed with diatomaceous earth (Extrelut®) was used. Chromatographic separation was achieved using an isocratic system with a mobile phase of trifluoroacetic acid-water-acetonitrile (0.01:90:10) applied at a flow rate of 1 mL min.-1. Validation studies were also carried out to show that the method is specific, accurate, and precise. In the tested specimens, the lycorine content ranged between 0.008 and 0.364% whereas the galanthamine content was found to be between 0.003 and 0.506%.

Keywords : alkaloids, Amaryllidaceae, Galanthus, galanthamine, lycorine,
Publishing house : University of Silesia in Katowice
Publication date : 2013
Number : Vol. 25, no. 4
Page : 755 – 764

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DOI :
Qute : Patil, A. N. ,Nirmal, S. A. ,Chavan, A. K. ,Zhou, L. ,Xu, J. ,Luan, L. ,Ma, J. ,Gong, Y. ,Qin, D. ,Pan, C. ,Jinlong, M. ,Guobin, J. ,Bo, J. ,Hua, J. ,Montejo, C. ,Civera, C. ,Barcia, E. ,Negro, S. ,Fernández-Carballido, A. ,Czauderna, M. ,Marounek, M. ,Duskova, D. ,Kowalczyk, J. ,Konda, R. K. ,Chandu, B. R. ,Challa, B. R. ,Chandrasekhar, K. B. ,Shen, Y. ,Yang, H. ,Xia, G. ,Wang, J. ,Cai, B. ,Jia, X. ,Nurulain, S. M. ,Kalász, H. ,Szegi, P. ,Kuca, K. ,Adem, A. ,Hasan, M. Y. B. ,Hashemi, F. ,Tekes, K. ,Sangoi, M. S. ,Wrasse-Sangoi, M. ,Hurtado, F. K. ,Rolim, C. M. B. ,Walorczyk, S. ,Li, W. ,Shi, L. L. ,Han, L. Q. ,Zhang, J. ,Mirjalili, M. H. ,Navarro, A. ,Hernández, L. ,Jáuregui, O. ,Bonfill, M. ,Emir, A. ,Cicek-Polat, D. ,Kaya, G. I. ,Sarikaya, B. ,Onur, M. A. ,Unver-Somer, N. ,Unver-Somer, N. , Simultaneous determination of lycorine and galanthamine in Galanthus woronowii by HPLC-DAD. Acta Chromatographica Vol. 25, no. 4/2013
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