Journal : Acta Chromatographica
Article : Simultaneous determination of nine flavonoids in beagle dog by HPLC with DAD and application of Ginkgo biloba extracts on the pharmacokinetic

Authors :
Xu, L. N.
Yantai University Department of Applied Chemistry Yantai 264005 P.R. China,
Gai, F. Y.
Yantai University Department of Applied Chemistry Yantai 264005 P.R. China,
Mu, G. F.
Yantai University Department of Applied Chemistry Yantai 264005 P.R. China,
Gao, Y.
Yantai University Department of Applied Chemistry Yantai 264005 P.R. China,
Liu, C. J.
Yantai University Department of Applied Chemistry Yantai 264005 P.R. China,
Luan, F.
Yantai University Department of Applied Chemistry Yantai 264005 P.R. China,
Mori, M.
Gunma University Department of Chemistry and Chemical Biology, Graduate School of Engineering 1-5-1 Tenjin-cho Kiryu 376-8515 Japan,
Ikeda, T.
Gunma University Department of Chemistry and Chemical Biology, Graduate School of Engineering 1-5-1 Tenjin-cho Kiryu 376-8515 Japan,
Itabashi, H.
Gunma University Department of Chemistry and Chemical Biology, Graduate School of Engineering 1-5-1 Tenjin-cho Kiryu 376-8515 Japan,
Soomro, R.
University of Sindh National Center of Excellence in Analytical Chemistry Jamshoro Pakistan,
Memon, S. Q.
University of Sindh Institute of Advance Research in Chemical Sciences Jamshoro Pakistan,
Ahmed, M. J.
University of Chittagong Laboratory of Analytical Chemistry, Department of Chemistry Chittagong 4331 Bangladesh,
Memon, N.
University of Sindh National Center of Excellence in Analytical Chemistry Jamshoro Pakistan, najmamemon@gmail.com,
Mallah, A.
University of Sindh M.A. Kazi Institute of Chemistry Jamshoro Pakistan,
Ravi, P. R.
BITS-Pilani Hyderabad Campus Pharmacy Department Ranga Reddy (Dist.) Jawaharnagar Andhra Pradesh India, rahulvats1983@gmail.com,
Aditya, N.
BITS-Pilani Hyderabad Campus Pharmacy Department Ranga Reddy (Dist.) Jawaharnagar Andhra Pradesh India, adityamurthy1212@gmail.com,
Vats, R.
BITS-Pilani Hyderabad Campus Pharmacy Department Ranga Reddy (Dist.) Jawaharnagar Andhra Pradesh India, rpunnarao@gmail.com,
Navaneethan, G.
Sona College of Technology (Anna University) Department of Sciences Salem 636 005 India,
Karunakaran, K.
Sona College of Technology (Anna University) Department of Sciences Salem 636 005 India, drkk@sonatech.ac.in,
Elango, K. P.
Gandhigram Rural Institute (Deemed University) Department of Chemistry Gandhigram 624 302 India,
Prichodko, A.
Vilnius University Department of Analytical and Environmental Chemistry Naugarduko 24 LT-03225 Vilnius Lithuania,
Janenaite, E.
Vilnius University Department of Analytical and Environmental Chemistry Naugarduko 24 LT-03225 Vilnius Lithuania,
Smitiene, V.
Vilnius University Department of Analytical and Environmental Chemistry Naugarduko 24 LT-03225 Vilnius Lithuania,
Vickackaite, V.
Vilnius University Department of Analytical and Environmental Chemistry Naugarduko 24 LT-03225 Vilnius Lithuania, vida.vickackaite@chf.vu.lt,
Akhtar, N.
Hamdard University Department of Pharmaceutics, Faculty of Pharmacy New Delhi 110062 India,
Faiyazuddin, M.
Hamdard University Department of Pharmaceutics, Faculty of Pharmacy New Delhi 110062 India, md.faiyazuddin@yahoo.co.in,
Mustafa, G.
Hamdard University Department of Pharmaceutics, Faculty of Pharmacy New Delhi 110062 India,
Sultana, Y.
Hamdard University Department of Pharmaceutics, Faculty of Pharmacy New Delhi 110062 India,
Baboota, S.
Hamdard University Department of Pharmaceutics, Faculty of Pharmacy New Delhi 110062 India,
Ali, J.
Hamdard University Department of Pharmaceutics, Faculty of Pharmacy New Delhi 110062 India,
Wang, D.
Shandong Academy of Sciences Shandong Analysis and Test Center 19 Keyuan Street Jinan 250014 P. R. China,
Bai, A.
inan Municipal Center For Disease Control and Prevention Jinan 250001 P. R. China,
Lin, X.
Shandong Academy of Sciences Shandong Analysis and Test Center 19 Keyuan Street Jinan 250014 P. R. China,
Fang, L.
Shandong Academy of Sciences Shandong Analysis and Test Center 19 Keyuan Street Jinan 250014 P. R. China,
Shu, X.
Shandong Academy of Sciences Shandong Analysis and Test Center 19 Keyuan Street Jinan 250014 P. R. China,
Shi, X.
Shandong Academy of Sciences Shandong Analysis and Test Center 19 Keyuan Street Jinan 250014 P. R. China,
Sun, Q.
Shandong Academy of Sciences Shandong Analysis and Test Center 19 Keyuan Street Jinan 250014 P. R. China,
Wang, X.
Shandong Academy of Sciences Shandong Analysis and Test Center 19 Keyuan Street Jinan 250014 P. R. China, wxjn1998@126.com,
Wu, J.
Xuzhou Medical College Key Laboratory of New Drug and Clinical Application Xuzhou Jiangsu 221004 China,
Xing, H.
Xuzhou Medical College Key Laboratory of New Drug and Clinical Application Xuzhou Jiangsu 221004 China,
Tang, D.
Xuzhou Medical College Key Laboratory of New Drug and Clinical Application Xuzhou Jiangsu 221004 China, tdq993@hotmail.com,
Gao, Y.
Xuzhou Medical College Key Laboratory of New Drug and Clinical Application Xuzhou Jiangsu 221004 China,
Yin, X.
Xuzhou Medical College Key Laboratory of New Drug and Clinical Application Xuzhou Jiangsu 221004 China,
Du, Q.
Xuzhou Medical College Key Laboratory of New Drug and Clinical Application Xuzhou Jiangsu 221004 China,
Jiang, X.
Xuzhou Medical College Key Laboratory of New Drug and Clinical Application Xuzhou Jiangsu 221004 China,
Yang, D.
Xuzhou Medical College Key Laboratory of New Drug and Clinical Application Xuzhou Jiangsu 221004 China,
Abstract : The method of high-performance liquid chromatography (HPLC) with diode array detector (DAD) was used and validated for the simultaneous determination of nine flavonoids (rutin, myricetin, quercitrin, quercetin, luteolin, genistein, kaempferol, apigenin, and isorhamnetin) in beagle dog plasma. Plasma sample was pre-treated with acetonitrile (containing 0.05% formic acid). Chromatographic separation was performed on a kromasil C18 column (250 × 4.6 mm, 5 µm) maintained at 35 °C. The mobile phase was a mixture of methanol and 0.2% formic acid with a step linear gradient. At 1.0 mL min-1flow rate, the eluent of other eight flavonoids was detected simultaneously at 360 nm with good separation except genistein (detected at 254 nm). Under optimum conditions, the correlation coefficient between the peak area and the concentrations for each analyte was all above 0.999. The intra-day and inter-day precisions were less than 10% for all analytes. The limit of detection and the limit of quantification for the selected nine flavonoids were 0.006–0.03 and 0.02–0.12 g mL,-1respectively. The extracted recoveries of selected nine flavonoids were 74.02%–99.37%. The assay has been successfully applied to determine concentrations of nine flavonoids in plasma from beagle dog after being intravenously administrated Ginkgo biloba extract.

Keywords : high-performance liquid chromatography, beagle dog plasma, flavonoid, Ginkgo biloba extract, pharmacokinetic,
Publishing house : University of Silesia in Katowice
Publication date : 2012
Number : Vol. 24, no. 4
Page : 627 – 642

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DOI :
Qute : Xu, L. N. ,Gai, F. Y. ,Mu, G. F. ,Gao, Y. ,Liu, C. J. ,Luan, F. ,Mori, M. ,Ikeda, T. ,Itabashi, H. ,Soomro, R. ,Memon, S. Q. ,Ahmed, M. J. ,Memon, N. ,Mallah, A. ,Ravi, P. R. ,Aditya, N. ,Vats, R. ,Navaneethan, G. ,Karunakaran, K. ,Elango, K. P. ,Prichodko, A. ,Janenaite, E. ,Smitiene, V. ,Vickackaite, V. ,Akhtar, N. ,Faiyazuddin, M. ,Mustafa, G. ,Sultana, Y. ,Baboota, S. ,Ali, J. ,Wang, D. ,Bai, A. ,Lin, X. ,Fang, L. ,Shu, X. ,Shi, X. ,Sun, Q. ,Wang, X. ,Wu, J. ,Xing, H. ,Tang, D. ,Gao, Y. ,Yin, X. ,Du, Q. ,Jiang, X. ,Yang, D. ,Yang, D. , Simultaneous determination of nine flavonoids in beagle dog by HPLC with DAD and application of Ginkgo biloba extracts on the pharmacokinetic. Acta Chromatographica Vol. 24, no. 4/2012
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