Simultaneous estimation of anti-cancer terpenoids in pharmaceutical nanoformulation by RP-HPLC and HPTLC

Czasopismo : Acta Chromatographica
Tytuł artykułu : Simultaneous estimation of anti-cancer terpenoids in pharmaceutical nanoformulation by RP-HPLC and HPTLC

Autorzy :
Mansoor, S.
Georgia Institute of Technology School of Chemical and Biomolecular Engineering Atlanta GA 30332-0100 USA,
Tas, C.
Georgia Institute of Technology School of Chemical and Biomolecular Engineering Atlanta GA 30332-0100 USA, ctas@gata.edu.tr,
Patel, D. S.
Pramukh Swami Science and H.D. Patel Arts College Chemistry Department Sarva Vidyalaya Campus Kadi 382 715 Gujarat India,
Sharma, N.
BA Research India Ltd. Bio-Analytical Laboratory Bodakdev, Ahmedabad 380054 Gujarat India,
Patel, M. C.
Pramukh Swami Science and H.D. Patel Arts College Chemistry Department Sarva Vidyalaya Campus Kadi 382 715 Gujarat India,
Patel, B. N.
BA Research India Ltd. Bio-Analytical Laboratory Bodakdev, Ahmedabad 380054 Gujarat India, bhavinpatel27@rediffmail.com,
Shrivastav, P. S.
Gujarat University Department of Chemistry, School of Sciences Navrangpura, Ahmedabad 380009 Gujarat India, pranav_shrivastav@yahoo.com,
Sanyal, M.
St. Xavier’s College Department of Chemistry Navrangpura, Ahmedabad 380009 Gujarat India,
Wang, G. Y.
Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P. R. China,
Shi, Y. P.
Lanzhou University of Technology School of Petroleum and Chemical Engineering Lanzhou 730050 P. R. China, shiyp@licp.cas.cn,
Akowuah, G. A.
UCSI University Faculty of Applied Sciences No. 1, Jalan Menara Gading, UCSI Heights 56000 Kuala Lumpur Malaysia, wuahmy@yahoo.com,
Okechukwu, P. N.
UCSI University Faculty of Pharmaceutical Sciences No. 1, Jalan Menara Gading, UCSI Heights 56000 Kuala Lumpur Malaysia,
Chiam, N. C.
UCSI University Faculty of Pharmaceutical Sciences No. 1, Jalan Menara Gading, UCSI Heights 56000 Kuala Lumpur Malaysia,
Raszewski, G.
Institute of Agricultural Medicine Department of Pathophysiology Lublin Poland, grasz@galen.imw.lublin.pl,
Juszczak, M.
Institute of Agricultural Medicine Department of Medical Biology Lublin Poland,
Lemieszek, M. K.
Institute of Agricultural Medicine Department of Medical Biology Lublin Poland,
Matysiak, J.
University of Life Sciences Department of Chemistry Lublin Poland,
Niewiadomy, A.
University of Life Sciences Department of Chemistry Lublin Poland,
Rzeski, W.
Maria Curie-Sklodowska University Department of Virology and Immunology, Institute of Microbiology and Biotechnology Lublin Poland,
Reddy, P. S.
IPDO Dr. Reddy’s Laboratories Ltd. Bachupally, Hyderabad 500072 A.P India, sunilpsr@yahoo.com,
Babu, K. S.
S. K. University Department of Chemistry Anantapur 515055 A.P. India,
Kumar, N.
IPDO Dr. Reddy’s Laboratories Ltd. Bachupally, Hyderabad 500072 A.P India,
Pareek, D.
Rajiv Gandhi Technological University School of Pharmaceutical Sciences Bhopal 462033 Madhya Pradesh India,
Jain, S.
Sagar Institute of Research and Technology Ayodhya bypass, Bhopal 462 041 MP India,
Basniwa, P. K.
Rajiv Gandhi Technological University School of Pharmaceutical Sciences Bhopal 462033 Madhya Pradesh India,
Jain, D.
Rajiv Gandhi Technological University School of Pharmaceutical Sciences Bhopal 462033 Madhya Pradesh India, deepti2515@yahoo.com,
Wróblewski, K.
Medical University of Lublin Department of Inorganic Chemistry 4a Chodźki Str. 20-093 Lublin Poland,
Petruczynik, A.
Medical University of Lublin Department of Inorganic Chemistry 4a Chodźki Str. 20-093 Lublin Poland,
Waksmundzka-Hajnos, M.
Medical University of Lublin Department of Inorganic Chemistry 4a Chodźki Str. 20-093 Lublin Poland, monika.hajnos@umlub.pl,
Jain, P. S.
R. C. Patel Institute of Pharmaceutical Education and Research Karwand Naka, Shirpur, Dist. Dhule 425 405 MS India, pritash79@yahoo.com,
Chaudhari, A. J.
R. C. Patel Institute of Pharmaceutical Education and Research Karwand Naka, Shirpur, Dist. Dhule 425 405 MS India,
Surana, S. J.
R. C. Patel Institute of Pharmaceutical Education and Research Karwand Naka, Shirpur, Dist. Dhule 425 405 MS India,
Elgorashe, R. E. E.
Sudan University of Science and Technology Department of Chemistry, College of Science Khartoum Sudan,
Idris, A. M.
King Khalid University Department of Chemistry, College of Science Abha Saudi Arabia, abubakridris@hotmail.com,
Abdelrahman, M. A.
Sudan University of Science and Technology Department of Chemistry, College of Science Khartoum Sudan,
Saeed, A. E. M.
Sudan University of Science and Technology Department of Chemistry, College of Science Khartoum Sudan,
Gawlik, M.
Jagiellonian University Medical College Department of Toxicology, Faculty of Pharmacy Medyczna str. 9 30-688 Kraków Poland,
Krzyżanowska, W.
Jagiellonian University Medical College Department of Toxicology, Faculty of Pharmacy Medyczna str. 9 30-688 Kraków Poland,
Gawlik, M. B.
Jagiellonian University Medical College Department of Toxicology, Faculty of Pharmacy Medyczna str. 9 30-688 Kraków Poland, mfgawlik@cyf-kr.edu.pl,
Filip, M.
Jagiellonian University Medical College Department of Toxicology, Faculty of Pharmacy Medyczna str. 9 30-688 Kraków Poland,
Tomsej, T.
VŠB-Technical University of Ostrava Institute of Clean Technologies 17. listopadu 15 708 33 Ostrava-Poruba Czech Republic, ttomsej@email.cz,
Tomsejova, S.
National Reference Laboratory for POP Institute of Public Health Ostrava Partyzanske namesti 7 702 00 Ostrava Czech Republic,
Dombek, V.
VŠB-Technical University of Ostrava Institute of Clean Technologies 17. listopadu 15 708 33 Ostrava-Poruba Czech Republic,
Maciejowska, A.
University of Silesia Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Godziek, A.
University of Silesia Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Sajewicz, M.
University of Silesia Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Kowalska, T.
University of Silesia Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland, teresa.kowalska@us.edu.pl,
Lainioti, G. C.
University of Patras Department of Chemistry GR-26504 Patras Greece,
Kapolos, J.
Technological Educational Institute of Kalamata Department of Food Technology 24100 Kalamata Greece,
Koliadima, A.
University of Patras Department of Chemistry GR-26504 Patras Greece,
Karaiskakis, G.
University of Patras Department of Chemistry GR-26504 Patras Greece, G.Karaiskakis@chemistry.upatras.gr,
Parveen, R.
Jamia Hamdard Department of Pharmaceutics, Faculty of Pharmacy New Delhi 110062 India,
Ahmad, F. J.
Jamia Hamdard Department of Pharmaceutics, Faculty of Pharmacy New Delhi 110062 India, farhanja2000@yahoo.com,
Iqbal, Z.
Jamia Hamdard Department of Pharmaceutics, Faculty of Pharmacy New Delhi 110062 India,
Singh, M.
Jamia Hamdard Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy New Delhi 110062 India,
Kamal, Y.
Jamia Hamdard Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy New Delhi 110062 India,
Ahmad, S.
Jamia Hamdard Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy New Delhi 110062 India, sahmad_jh@yahoo.co.in,
Abstrakty : Andrographolide and betulinic acid are the terpenoids having potential anti-cancer activity. The cytotoxicity activity of both the drugs was carried out separately and in combination on liver cancer HepG2 cell lines. High-performance liquid chromatography (HPLC) and high-performance thin-layer chromatography (HPTLC) methods were developed and validated for simultaneous estimation of these two terpenoids as per the International Conference on Harmonization (ICH) guidelines, which was applied for quantification in nanoformulation. The retention time by HPLC and retardation factor by HPTLC for andrographolide and betulinic acid were found to be 2.2 and 6.6 min, and 0.24 ± 0.01 and 0.66 ± 0.01, respectively. Both the methods were validated for accuracy, precision, repeatability, robustness, limit of detection (LOD), and limit of quantitation (LOQ). The content of andrographolide and betulinic acid in nanoformulation was found to be 96.0% and 98.0% by HPLC and 96.59% and 98.33% by HPTLC, respectively, of labelled claim.

Słowa kluczowe : andrographolide, betulinic acid, high-performance liquid chromatography, high-performance thin-layer chromatography,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2014
Numer : Vol. 26, no. 2
Strony : 391 – 400
Bibliografia : 1 A. Varma, H. Padh, and N. Shrivastava, eCAM, 1–9 (2011), Article ID 815390. doi: 10.1093/ecam/nep135
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DOI :
Cytuj : Mansoor, S. ,Tas, C. ,Patel, D. S. ,Sharma, N. ,Patel, M. C. ,Patel, B. N. ,Shrivastav, P. S. ,Sanyal, M. ,Wang, G. Y. ,Shi, Y. P. ,Akowuah, G. A. ,Okechukwu, P. N. ,Chiam, N. C. ,Raszewski, G. ,Juszczak, M. ,Lemieszek, M. K. ,Matysiak, J. ,Niewiadomy, A. ,Rzeski, W. ,Reddy, P. S. ,Babu, K. S. ,Kumar, N. ,Pareek, D. ,Jain, S. ,Basniwa, P. K. ,Jain, D. ,Wróblewski, K. ,Petruczynik, A. ,Waksmundzka-Hajnos, M. ,Jain, P. S. ,Chaudhari, A. J. ,Surana, S. J. ,Elgorashe, R. E. E. ,Idris, A. M. ,Abdelrahman, M. A. ,Saeed, A. E. M. ,Gawlik, M. ,Krzyżanowska, W. ,Gawlik, M. B. ,Filip, M. ,Tomsej, T. ,Tomsejova, S. ,Dombek, V. ,Maciejowska, A. ,Godziek, A. ,Sajewicz, M. ,Kowalska, T. ,Lainioti, G. C. ,Kapolos, J. ,Koliadima, A. ,Karaiskakis, G. ,Parveen, R. ,Ahmad, F. J. ,Iqbal, Z. ,Singh, M. ,Kamal, Y. ,Ahmad, S. , Simultaneous estimation of anti-cancer terpenoids in pharmaceutical nanoformulation by RP-HPLC and HPTLC. Acta Chromatographica Vol. 26, no. 2/2014
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