Simultaneous identification and quantitative determination of azithromycin, clarithromycin, roxithromycin, spiramycin and troleandomycin by thin-layer chromatography and densitometry

Czasopismo : Acta Chromatographica
Tytuł artykułu : Simultaneous identification and quantitative determination of azithromycin, clarithromycin, roxithromycin, spiramycin and troleandomycin by thin-layer chromatography and densitometry

Autorzy :
Kuc, J.
Cracow University of Technology Department of Chemical Engineering and Technology, Department of Analytical Chemistry Warszawska 24 31-155 Cracow Poland, jkuc@chemia.pk.edu.pl,
Grochowalski, A.
Cracow University of Technology Department of Chemical Engineering and Technology, Department of Analytical Chemistry Warszawska 24 31-155 Cracow Poland,
Mach, S.
Institute of Public Health Ostrava Centre of Hygienic Laboratories Dobrá 240 739 51 Frýdek-Místek Czech Republic,
Placha, D.
VŠB-Technical University of Ostrava Centrum Nanotechnology 17 Listopadu 15/2172 708 33 Ostrava Czech Republic,
Furusawa, N.
Osaka City University Graduate School of Human Life Science Osaka 558-8585 Japan,
Stoilova, N.
Bulgarian Food Safety Agency Central Laboratory of Veterinary Control and Ecology (CLVCE) 5 “Iskarsko shousse” str., 1528 Sofia Bulgaria, nadqstoilova@abv.bg,
Surleva, A.
University of Chemical Technology and Metallurgy Analytical Chemistry Department 8 “St. Kl. Ohridski” blvd., 1756 Sofia Bulgaria,
Stoev, G.
Bulgarian Food Safety Agency Central Laboratory of Veterinary Control and Ecology (CLVCE) 5 “Iskarsko shousse” str., 1528 Sofia Bulgaria,
Popovic, N.
Lafayette College Department of Chemistry Easton PA USA,
Sherma, J.
Lafayette College Department of Chemistry Easton PA USA, shermaj@lafayette.edu,
Dixit, S.
Yeungnam University Department of Chemistry Gyeongsan 712-749 South Korea, drdixit.shuchi@gmail.com,
Dubey, R.
riturajdubey0@gmail.com,
Bhushan, R.
Indian Institute of Technology Roorkee Department of Chemistry Roorkee 247667 India, rbushfcy@iitr.ernet.in,
Fouad, M. A.
Cairo University Pharmaceutical Chemistry Department, Faculty of Pharmacy Kasr El-Aini St. Cairo 11562 Egypt, merro911@yahoo.com,
Elkady, E. F.
Cairo University Pharmaceutical Chemistry Department, Faculty of Pharmacy Kasr El-Aini St. Cairo 11562 Egypt,
Csomor, V.
Semmelweis University Department of Pharmacodynamics Budapest Hungary,
Laufer, R.
Semmelweis University Department of Pharmacodynamics Budapest Hungary,
Pöstényi, Z.
Semmelweis University Department of Pharmacology and Pharmacotherapy Budapest Hungary,
Kalász, H.
Semmelweis University Department of Pharmacology and Pharmacotherapy Budapest Hungary, huba.kalasz@gmail.com,
Adeghate, E.
United Arab Emirates University Department of Anatomy Al Ain UAE,
Nurulain, S. M.
United Arab Emirates University Department of Pharmacology and Therapeutics Al Ain UAE,
Tekes, K.
Semmelweis University Department of Pharmacodynamics Budapest Hungary,
Adem, A.
United Arab Emirates University Department of Pharmacology and Therapeutics Al Ain UAE,
Kwiecień, A.
Jagiellonian University Medical College, Faculty of Pharmacy Department of Inorganic and Analytical Chemistry 9 Medyczna Street 30-688 Kraków Poland,
Krzek, J.
Jagiellonian University Medical College, Faculty of Pharmacy Department of Inorganic and Analytical Chemistry 9 Medyczna Street 30-688 Kraków Poland, jankrzek@cm-uj.krakow.pl,
Gądek, M.
Jagiellonian University Medical College, Faculty of Pharmacy Department of Inorganic and Analytical Chemistry 9 Medyczna Street 30-688 Kraków Poland,
Abstrakty : The purpose of this work was to develop a TLC-densitometric method for simultaneous identification and quantitative determination of azithromycin, clarithromycin, roxithromycin, spiramycin, and troleandomycin. The method was developed on TLC aluminium plates precoated with silica gel F254 using solvent system izopropanol:n-hexane:ammonia 25% (8:12:3, v/v/v), which gives compact spots for azithromycin (RF = 0.65), clarithromycin (RF = 0.54), roxithromycin (RF = 0.49), spiramycin (RF = 0.22), and troleandomycin (RF = 0.36). Densitometric analysis was carried out at 478 nm after spraying with (1:4, v/v) sulphuric acid:ethanol and heating at 100°C for 5 min. The linear regression analysis data for the calibration plots showed good linear relationship with correlation coefficient higher than 0.99. The method is distinguished by high sensitivity, with limit of detection (LOD) from 0.34 μg/spot for troleandomycin to 0.67 μg/spot for clarithromycin and limit of quantification (LOQ) from 1.02 μg/spot for troleandomycin to 2.04 μg/spot for clarithromycin and a wide linearity range from 2 to 12 μg/spot for spiramycin and 2–15 μg/spot for other antibiotics. The precision of the determination was good; relative standard deviation (RSD) varied in the range from 1.49% to 4.14%.

Słowa kluczowe : macrolide antibiotics, pharmaceutical research, method validation, thin-layer chromatography, densitometry,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2014
Numer : Vol. 26, no. 4
Strony : 657 – 670
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DOI :
Cytuj : Kuc, J. ,Grochowalski, A. ,Mach, S. ,Placha, D. ,Furusawa, N. ,Stoilova, N. ,Surleva, A. ,Stoev, G. ,Popovic, N. ,Sherma, J. ,Dixit, S. ,Dubey, R. ,Bhushan, R. ,Fouad, M. A. ,Elkady, E. F. ,Csomor, V. ,Laufer, R. ,Pöstényi, Z. ,Kalász, H. ,Adeghate, E. ,Nurulain, S. M. ,Tekes, K. ,Adem, A. ,Kwiecień, A. ,Krzek, J. ,Gądek, M. , Simultaneous identification and quantitative determination of azithromycin, clarithromycin, roxithromycin, spiramycin and troleandomycin by thin-layer chromatography and densitometry. Acta Chromatographica Vol. 26, no. 4/2014
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