Journal : Acta Chromatographica
Article : Simultaneous quantification of salvianolic acid B and tanshinone IIA of salvia tropolone tablets by UPLC-MRM-MS/MS for pharmacokinetic studies

Authors :
Kuc, J.
Cracow University of Technology Department of Chemical Engineering and Technology, Department of Analytical Chemistry Warszawska 24 31-155 Cracow Poland, jkuc@chemia.pk.edu.pl,
Grochowalski, A.
Cracow University of Technology Department of Chemical Engineering and Technology, Department of Analytical Chemistry Warszawska 24 31-155 Cracow Poland,
Mach, S.
Institute of Public Health Ostrava Centre of Hygienic Laboratories Dobrá 240 739 51 Frýdek-Místek Czech Republic,
Placha, D.
VŠB-Technical University of Ostrava Centrum Nanotechnology 17 Listopadu 15/2172 708 33 Ostrava Czech Republic,
Furusawa, N.
Osaka City University Graduate School of Human Life Science Osaka 558-8585 Japan,
Stoilova, N.
Bulgarian Food Safety Agency Central Laboratory of Veterinary Control and Ecology (CLVCE) 5 “Iskarsko shousse” str., 1528 Sofia Bulgaria, nadqstoilova@abv.bg,
Surleva, A.
University of Chemical Technology and Metallurgy Analytical Chemistry Department 8 “St. Kl. Ohridski” blvd., 1756 Sofia Bulgaria,
Stoev, G.
Bulgarian Food Safety Agency Central Laboratory of Veterinary Control and Ecology (CLVCE) 5 “Iskarsko shousse” str., 1528 Sofia Bulgaria,
Popovic, N.
Lafayette College Department of Chemistry Easton PA USA,
Sherma, J.
Lafayette College Department of Chemistry Easton PA USA, shermaj@lafayette.edu,
Dixit, S.
Yeungnam University Department of Chemistry Gyeongsan 712-749 South Korea, drdixit.shuchi@gmail.com,
Dubey, R.
riturajdubey0@gmail.com,
Bhushan, R.
Indian Institute of Technology Roorkee Department of Chemistry Roorkee 247667 India, rbushfcy@iitr.ernet.in,
Fouad, M. A.
Cairo University Pharmaceutical Chemistry Department, Faculty of Pharmacy Kasr El-Aini St. Cairo 11562 Egypt, merro911@yahoo.com,
Elkady, E. F.
Cairo University Pharmaceutical Chemistry Department, Faculty of Pharmacy Kasr El-Aini St. Cairo 11562 Egypt,
Csomor, V.
Semmelweis University Department of Pharmacodynamics Budapest Hungary,
Laufer, R.
Semmelweis University Department of Pharmacodynamics Budapest Hungary,
Pöstényi, Z.
Semmelweis University Department of Pharmacology and Pharmacotherapy Budapest Hungary,
Kalász, H.
Semmelweis University Department of Pharmacology and Pharmacotherapy Budapest Hungary, huba.kalasz@gmail.com,
Adeghate, E.
United Arab Emirates University Department of Anatomy Al Ain UAE,
Nurulain, S. M.
United Arab Emirates University Department of Pharmacology and Therapeutics Al Ain UAE,
Tekes, K.
Semmelweis University Department of Pharmacodynamics Budapest Hungary,
Adem, A.
United Arab Emirates University Department of Pharmacology and Therapeutics Al Ain UAE,
Kwiecień, A.
Jagiellonian University Medical College, Faculty of Pharmacy Department of Inorganic and Analytical Chemistry 9 Medyczna Street 30-688 Kraków Poland,
Krzek, J.
Jagiellonian University Medical College, Faculty of Pharmacy Department of Inorganic and Analytical Chemistry 9 Medyczna Street 30-688 Kraków Poland, jankrzek@cm-uj.krakow.pl,
Gądek, M.
Jagiellonian University Medical College, Faculty of Pharmacy Department of Inorganic and Analytical Chemistry 9 Medyczna Street 30-688 Kraków Poland,
Kuźma, Ł.
Medical University of Łódź Department of Biology and Pharmaceutical Botany Muszyńskiego 1 st. 90-151 Łódź Poland, lukasz.kuzma@umed.lodz.p,
Wysokińska, H.
Medical University of Łódź Department of Biology and Pharmaceutical Botany Muszyńskiego 1 st. 90-151 Łódź Poland,
Dong, H.
Harbin Engineering University Polymer Materials Research Center, Key Laboratory of Superlight Materials and Surface Technology Ministry of Education. College of Material Science and Chemical Engineering Harbin 150001 China, hongxingd6@yahoo.com.cn,
Wang, Y.
Harbin Engineering University Polymer Materials Research Center, Key Laboratory of Superlight Materials and Surface Technology Ministry of Education. College of Material Science and Chemical Engineering Harbin 150001 China,
Ou, Y.
Harbin Engineering University Polymer Materials Research Center, Key Laboratory of Superlight Materials and Surface Technology Ministry of Education. College of Material Science and Chemical Engineering Harbin 150001 China,
She, J.
Harbin Engineering University Polymer Materials Research Center, Key Laboratory of Superlight Materials and Surface Technology Ministry of Education. College of Material Science and Chemical Engineering Harbin 150001 China,
Shen, X.
Harbin Engineering University Polymer Materials Research Center, Key Laboratory of Superlight Materials and Surface Technology Ministry of Education. College of Material Science and Chemical Engineering Harbin 150001 China,
Li, J.
Harbin Engineering University Polymer Materials Research Center, Key Laboratory of Superlight Materials and Surface Technology Ministry of Education. College of Material Science and Chemical Engineering Harbin 150001 China,
Zhang, C.
Harbin Engineering University Polymer Materials Research Center, Key Laboratory of Superlight Materials and Surface Technology Ministry of Education. College of Material Science and Chemical Engineering Harbin 150001 China,
Liu, L.
Harbin Engineering University Polymer Materials Research Center, Key Laboratory of Superlight Materials and Surface Technology Ministry of Education. College of Material Science and Chemical Engineering Harbin 150001 China,
Xiong, W.
Tianjin University of Traditional Chinese Medicine Tianjin State Key Laboratory of Modern Chinese Medicine 300193 Tianjin PR China,
Yan, R. Q.
Tianjin University of Traditional Chinese Medicine Tianjin State Key Laboratory of Modern Chinese Medicine 300193 Tianjin PR China,
Liu, Y. N.
Tianjin University of Traditional Chinese Medicine Tianjin State Key Laboratory of Modern Chinese Medicine 300193 Tianjin PR China,
Peng, S. W.
Tianjin University of Traditional Chinese Medicine Tianjin State Key Laboratory of Modern Chinese Medicine 300193 Tianjin PR China,
Jiang, Z. Z.
Tianjin University of Traditional Chinese Medicine Tianjin State Key Laboratory of Modern Chinese Medicine 300193 Tianjin PR China,
Chai, X.
Tianjin University of Traditional Chinese Medicine Tianjin State Key Laboratory of Modern Chinese Medicine 300193 Tianjin PR China,
Qi, A. D.
Tianjin University of Traditional Chinese Medicine Tianjin State Key Laboratory of Modern Chinese Medicine 300193 Tianjin PR China,
Wang, Y. F.
Tianjin University of Traditional Chinese Medicine Tianjin State Key Laboratory of Modern Chinese Medicine 300193 Tianjin PR China, wangyuefei_2006@hotmail.com,
Li, J. R.
Chinese Academy of Sciences Chengdu Institute of Biology Chengdu 610041 PR China,
Li, D. X.
Sichuan Academy of Chinese Medicine Sciences Sichuan Key Laboratory of Quality and Innovation Research of Chinese Materia Medica Chengdu 610041 PR China,
Li, L.
Waters China Limited Block 41, 1387 Zhangdong Road, Pudong New District Shanghai 201203 PR China,
Deng, W. L.
Sichuan Academy of Chinese Medicine Sciences Sichuan Key Laboratory of Quality and Innovation Research of Chinese Materia Medica Chengdu 610041 PR China,
Ding, L. S.
Chinese Academy of Sciences Chengdu Institute of Biology Chengdu 610041 PR China,
Xu, H. X.
Shanghai University of Traditional Chinese Medicines Shanghai 201203 PR China,
Zhou, Y.
Chinese Academy of Sciences Chengdu Institute of Biology Chengdu 610041 PR China, zhouyan@cib.ac.cn,
Abstract : A rapid and sensitive ultraperformance liquid chromatography-multiple reaction monitoring-multi-stage/mass spectrometry (UPLC-MRM-MS/MS) method has been developed for simultaneous quantification of salvianolic acid B and tanshinone IIA of salvia tropolone tablets in dog plasma. This was achieved by performing quantification using the MRM acquisition with two channels of MRM-MS/MS and MS full scan for more accuracy qualitative results, and the fragmentation transitions of m/z 295→249, 191 for tanshinone IIA and m/z 297→279, 251 for IS in positive mode, m/z 717→519, 321 for salvianolic acid B and m/z 295→267, 239 for IS in negative mode were selected. The UPLC separation was achieved within 3 min in a single UPLC run. Linear calibration curves were obtained over the concentration range of 10 pg/mL-1ng/mL for tanshinone IIA and 100 pg/mL-1for salvianolic acid B. Lower limit of quantitation (LLOQ) was 10 pg/mL and 100 pg/mL for tanshinone IIA and salvianolic acid B, respectively. The inter-day and intra-day precision (relative standard deviation, RSD) in all samples were less than 8.21%, and the recoveries were over 85.9% for both tanshinone IIA and salvianolic acid B. The two channels of MRM with MS full scan approach could provide both qualitative and quantitative results without the need for repetitive analyses and resulted in the reduction of further confirmation experiments and analytical time. The pharmacokinetic study of the two active components of salvia tropolone tablets following oral gavage administration of dogs was thus explored with this method.

Keywords : salvia tropolone tablets, tanshinone IIA, salvianolic acid B, UPLC-MRM-MS/MS, pharmacokinetic study,
Publishing house : University of Silesia in Katowice
Publication date : 2014
Number : Vol. 26, no. 4
Page : 711 – 725

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DOI :
Qute : Kuc, J. ,Grochowalski, A. ,Mach, S. ,Placha, D. ,Furusawa, N. ,Stoilova, N. ,Surleva, A. ,Stoev, G. ,Popovic, N. ,Sherma, J. ,Dixit, S. ,Dubey, R. ,Bhushan, R. ,Fouad, M. A. ,Elkady, E. F. ,Csomor, V. ,Laufer, R. ,Pöstényi, Z. ,Kalász, H. ,Adeghate, E. ,Nurulain, S. M. ,Tekes, K. ,Adem, A. ,Kwiecień, A. ,Krzek, J. ,Gądek, M. ,Kuźma, Ł. ,Wysokińska, H. ,Dong, H. ,Wang, Y. ,Ou, Y. ,She, J. ,Shen, X. ,Li, J. ,Zhang, C. ,Liu, L. ,Xiong, W. ,Yan, R. Q. ,Liu, Y. N. ,Peng, S. W. ,Jiang, Z. Z. ,Chai, X. ,Qi, A. D. ,Wang, Y. F. ,Li, J. R. ,Li, D. X. ,Li, L. ,Deng, W. L. ,Ding, L. S. ,Xu, H. X. ,Zhou, Y. ,Zhou, Y. , Simultaneous quantification of salvianolic acid B and tanshinone IIA of salvia tropolone tablets by UPLC-MRM-MS/MS for pharmacokinetic studies. Acta Chromatographica Vol. 26, no. 4/2014
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