Suitability of the Chromdes DS-L horizontal TLC chamber for temperature-controlled planar chromatography

Czasopismo : Acta Chromatographica
Tytuł artykułu : Suitability of the Chromdes DS-L horizontal TLC chamber for temperature-controlled planar chromatography

Autorzy :
Bartnik, M.
Department of Pharmacognosy with Medicinal Plant Laboratory, Medical University, 1, Chodźki Str., 20-093 Lublin, Poland,
Kowalski, R.
Subdepartment of Food Quality Assessment and Central Apparatus Laboratory, Agricultural University, 20-950 Lublin, Akademicka 13, Poland,
Polak, B.
Department of Physical Chemistry, Faculty of Pharmacy, Medical University, Lublin, Poland,
Turło, J.
Department of Drug Technology, Medical University of Warsaw, 1, Banacha Street, 02-097 Warsaw, Poland,
Szyrwińska, K.
Department of Inorganic and Analytical Chemistry, Faculty of Pharmacy, Poznań University of Medical Sciences, Grunwaldzka 6, 60-780 Poznań, Poland,
Czauderna, M.
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland,
Bączek, T.
Medical University of Gdańsk, Department of Biopharmaceutics and Pharmacodynamics, Gdańsk, Poland,
Chilmonczyk, Z.
National Medicines Institute, 30/34, Chełmska Str., 00-725 Warsaw, Poland,
Hung, Ch. Y.
Department of Biotechnology, National Formosa University, Huwei, Yunlin, 632, Taiwan,
Baboota, S.
Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, Hamdard Nagar, New Delhi-110062, India,
Sivakumar, T.
Department of Pharmacy, Annamalai University, Annamalai Nagar, Tamil Nadu-608 002, India,
Ivanović, D.
Faculty of Pharmacy, Department of Drug Analysis, Vojvode Stepe 450, 11000 Belgrade, Serbia,
Pavlova, V.
Institute of Chemistry, Faculty of Natural Sciences and Mathematics, Saints Cyril and Methodius University, Arhimedova 5, P.O. Box 162, 1000 Skopje, Macedonia,
Ali, J.
Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, New Delhi-110062, India,
Nabi, S. A.
Analytical Research Laboratory, Department of Chemistry, Faculty of Science, Aligarh Muslim University, Aligarh-202002, India,
Murray, K. E.
Department of Chemistry, Lafayette College, Easton, PA 18042, USA,
Antić, D.
Institute of Pharmaceutical Chemistry and Drug Analysis, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000 Belgrade, Serbia,
Jóźwiak, G. W.
Department of Inorganic Chemistry, Medical University of Lublin, Staszica 6, 20-081 Lublin, Poland,
Purghazi, K.
Department of Applied Chemistry, Faculty of Chemistry, University of Tarbiat Moallem, 49 Mofatteh Avenue, Tehran 15614, Iran,
Sajewicz, M.
Institute of Chemistry, Silesian University, 9 Szkolna Street, 40-006 Katowice, Poland,
Mohammad, A.
Analytical Research Laboratory, Department of Applied Chemistry, Faculty of Engineering and Technology, Aligarh Muslim University, Aligarh-202002, India,
Zarzycki, P. K.
Laboratory of Toxicology, Department of Environmental Biology, Koszalin University of Technology, Śniadeckich 2, 75-453 Koszalin, Poland,
Abstrakty : Isothermal thin-layer chromatography in a non-thermostatted horizontal chamber has been investigated at sub-ambient and elevated temperatures. The rate of temperature equilibration was determined for the commercially available Chromdes DS-L horizontal TLC chamber placed in a circulating-air incubator. The time required for temperature equilibration was also studied for commonly used glass and aluminium backed plates transferred from room temperature to a chamber at temperatures ranging from 5 to 40°C. The effect of temperature on the retention and separation the steroids cholesterol, ergosterol, and stigmasterol was also investigated. Practical aspects of system robustness, temperature distribution within the chromatographic chamber, the stability of plate temperature, and applications of the method are also discussed.

Słowa kluczowe :
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2007
Numer : No. 18
Strony : 249 – 259
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DOI :
Cytuj : Bartnik, M. ,Kowalski, R. ,Polak, B. ,Turło, J. ,Szyrwińska, K. ,Czauderna, M. ,Bączek, T. ,Chilmonczyk, Z. ,Hung, Ch. Y. ,Baboota, S. ,Sivakumar, T. ,Ivanović, D. ,Pavlova, V. ,Ali, J. ,Nabi, S. A. ,Murray, K. E. ,Antić, D. ,Jóźwiak, G. W. ,Purghazi, K. ,Sajewicz, M. ,Mohammad, A. ,Zarzycki, P. K. , Suitability of the Chromdes DS-L horizontal TLC chamber for temperature-controlled planar chromatography. Acta Chromatographica No. 18/2007
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