Article : TLC analysis of glucose and maltose in Biomphalaria glabrata (Gastropoda) infected with Schistosoma mansoni (Trematoda)
Authors : Kowalska, T.Institute of Chemistry, Silesian University, 9 Szkolna Street, 40-006 Katowice, Poland, Kaczmarski, K.Chemical Engineering Department, Rzeszów University of Technology, Rzeszów, Poland, Pavlova, A.Analytical Department, Research and Development Institute, Lukoil-Neftochim-Bourgas-AD, 8104 Bourgas, Bulgaria, Giza, I.Plant Protection Institute, Sośnicowice Branch, ul. Gliwicka 29, 44-153 Sośnicowice, Poland, Walorczyk, S.Institute of Plant Protection, Department of Pesticide Residue Research, ul. Miczurina 20, 60-318 Poznań, Poland, Waksmundzka-Hajnos, M.Department of Inorganic and Analytical Chemistry, Medical University, Staszica 6, 20-081 Lublin, Poland, Gadzikowska, M.Department of Inorganic and Analytical Chemistry, Medical Academy, Staszica 6, 20-081 Lublin, Poland, Nagahashi, M.Laboratory of Instrumental Analysis, Tokyo University of Agriculture, 1-1, Sakuragaoka-1, Setagaya, Tokyo 156-8502, Japan, Fisher, J.Department of Chemistry, Lafayette College, Easton, PA 18042, USA, Schariter, J. J.Department of Chemistry, Lafayette College, Easton, PA 18042, USA,
Abstract : Analysis by thin-layer chromatography (TLC) has been performed on the hemolymph and digestive gland-gonad complex (DDG) of Biompha-laria glabrata snails experimentally infected with Schistosoma mansoni larvae. The major sugars identified in both the hemolymph and DGG of infected and uninfected snails were glucose and maltose. Densitometric quantification showed that there was a significant decrease (Student's t-test, P<0.05) in levels of glucose and maltose in the hemolymph of infected snails 5 and 7 weeks post-infection. At week 7 after infection there was a significant decrease in maltose in the DGG of infected snails. Findings from this work were compared with results previously obtained from spectrophotometric analysis of glucose in B. glabrata infected with larval S. mansoni. It was found that spectrophotometric analysis was less selective and reliable than TLC.
Publishing house : University of Silesia in Katowice
Publication date : 2001
Number : No. 11
Page : 102 – 107
Qute : Kowalska, T. ,Kaczmarski, K. ,Pavlova, A. ,Giza, I. ,Walorczyk, S. ,Waksmundzka-Hajnos, M. ,Gadzikowska, M. ,Nagahashi, M. ,Fisher, J. ,Schariter, J. J. ,Schariter, J. J. , TLC analysis of glucose and maltose in Biomphalaria glabrata (Gastropoda) infected with Schistosoma mansoni (Trematoda). Acta Chromatographica No. 11/2001