Journal : Acta Chromatographica
Article : Thermodynamic study of urobilinogenic chlorophyll catabolites by reversed-phase liquid chromatography

Authors :
Komsta, Ł.
Medical University of Lublin Chair and Department of Medicinal Chemistry, Faculty of Pharmacy Jaczewskiego 4 20-090 Lublin Poland, lukasz.komsta@am.lublin.pl,
Gowin, E.
Medical University of Lublin Chair and Department of Medicinal Chemistry, Faculty of Pharmacy Jaczewskiego 4 20-090 Lublin Poland,
Djordjević Filijović, N.
Hemofarm AD Vrsac Serbia,
Pavlović, A.
Hemofarm AD Vrsac Serbia,
Nikolić, K.
University of Belgrade, Faculty of Pharmacy Department of Pharmaceutical Chemistry Vojvode Stepe 450 PO Box 146 11000 Belgrade Serbia,
Agbaba, D.
University of Belgrade, Faculty of Pharmacy Department of Pharmaceutical Chemistry Vojvode Stepe 450 PO Box 146 11000 Belgrade Serbia, danica@pharmacy.bg.ac.rs,
Sawant, S.
Sinhgad Technical Education Society’s Smt. Kashibai Navale College of Pharmacy, Kondhwa (Bk.) Pune 411 048 Maharashtra India, sawant99@rediffmail.com,
Barge, V.
PDEA’s Seth Govind Raghunath Sable College of Pharmacy Saswad, Tal-Purandar, Dist-Pune 412301 Maharashtra India,
Tiwari, R.
School of Pharmacy and Technology Department of Pharmaceutical Chemistry, SVKM’s NMIMS Management Shirpur Dist. Dhule 425405 Maharashtra India, ravisun4@rediffmail.com,
Bonde, C.
School of Pharmacy and Technology Department of Pharmaceutical Chemistry, SVKM’s NMIMS Management Shirpur Dist. Dhule 425405 Maharashtra India,
Bothara, K.
Sinhgad Institute of Pharmacy Department of Pharmaceutical Chemistry Narhe Road Pune 410041 Maharashtra India,
Nawaz, M.
United Arab Emirates University Department of Chemistry Al-Ain 17551 UAE, nawwaz@gmail.com,
Arayne, M. S.
University of Karachi Department of Chemistry Karachi 75270 Pakistan,
Sultana, N.
University of Karachi Research Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, Faculty of Pharmacy Karachi 75270 Pakistan,
Haider, A.
University of Karachi Department of Chemistry Karachi 75270 Pakistan,
Hisaindee, S.
United Arab Emirates University Department of Chemistry Al-Ain 17551 UAE,
Youssef, R. M.
University of Alexandria Faculty of Pharmacy, Department of Pharmaceutical Analytical Chemistry El-Messalah, Alexandria 21521 Egypt, rmmy1973@yahoo.com,
Malesevic, M.
Medicines and Medical Devices Agency of Serbia Vojvode Stepe 458 11 152 Belgrade Serbia,
Zivanovic, L.
University of Belgrade Faculty of Pharmacy, Department of Drug Analysis Vojvode Stepe 450 11 152 Belgrade Serbia, ljzivan@pharmacy.bg.ac.rs,
Protic, A.
University of Belgrade Faculty of Pharmacy, Department of Drug Analysis Vojvode Stepe 450 11 152 Belgrade Serbia,
Radisic, M.
University of Belgrade Faculty of Technology and Metallurgy, Department of Analytical Chemistry 11000 Belgrade Serbia,
Lausevic, M.
University of Belgrade Faculty of Technology and Metallurgy, Department of Analytical Chemistry 11000 Belgrade Serbia,
Jovic, Z.
Medicines and Medical Devices Agency of Serbia Vojvode Stepe 458 11 152 Belgrade Serbia,
Jóźwiak, A.
Department of Inorganic Chemistry Medical University of Lublin 20-093 Lublin Poland,
Jóźwiak, G.
Department of Inorganic Chemistry Medical University of Lublin 20-093 Lublin Poland,
Waksmundzka-Hajnos, M.
Department of Inorganic Chemistry Medical University of Lublin 20-093 Lublin Poland, monika.hajnos@am.lublin.pl,
Djapic, N.
Mihajlo Pupin Djure Djakovica bb 23000 Zrenjanin Serbia, ninadjapic@yahoo.com,
Abstract : The urobilinogenic chlorophyll catabolites are secondary metabolites formed by the biodegradation of chlorophylls. The thermodynamic study of the urobilinogenic chlorophyll catabolites from autumnal leaves extract of Parrotia persica and Hamamelis virginiana was done using reversed-phase liquid chromatography on the C4 and C8 analytical columns with acidified water-methanol mobile phase in combination with ultraviolet detection and electrospray ionization mass spectrometry. The presence of the urobilinogenic chlorophyll catabolites was detected by their characteristic ultraviolet absorption and their molecular mass. The retention behaviors of the two urobilinogenic chlorophyll catabolite isomers over a temperature range of 278–318 K were investigated. The retention time data permitted the construction of the van’t Hoff plots. The stationary phase composition influences the thermodynamics of the retention of the urobilinogenic chlorophyll catabolites. The study presented can find the application in the separation of the urobilinogenic chlorophyll catabolites in autumnal leaves extracts.

Keywords : urobilinogenic chlorophyll catabolites, liquid chromatography, thermodynamics,
Publishing house : University of Silesia in Katowice
Publication date : 2014
Number : Vol. 26, no. 1
Page : 111 – 122

Bibliography
: 1 N. Djapic and M. Pavlovic, Rev. Chim. (Bucuresti), 59, 878 (2008).
2 N. Djapic, M. Pavlovic, S. Arsovski, and G. Vujic, Rev. Chim. (Bucuresti), 60, 398 (2009).
3 N. Djapic, A. Djuric, and A. Pavlovic, Res. J. Agr. Sci., 41, 256 (2009).
4 B. Kraeutler in The Porphyrin Handbook 13, 2003, pp. 183–209.
5 M. Oberhuber, J. Berghold, K. Breuker, S. Hoertensteiner, and B. Kraeutler, Proc. Natl. Acad. Sci. USA, 100, 6910 (2003).
6 T. Mueller, S. Oradu, D. R. Ifa, R. G. Cooks, and B. Kraeutler, Anal. Chem., 83, 5754 (2011).
7 C. B. Castells and P. W. Carr, Chromatographia, 52, 535 (2000).
8 R. E. Ardrey, Liquid Chromatography-Mass Spectrometry: An Introduction, Wiley, 2003, 18
9 A. Tchapla, S. Heron, H. Colin, and G. Guichon, Anal. Chem., 60, 1443 (1988).
10 S. C. Ringwald and J. E. Pemberton, Environ. Sci. Technol., 34, 259 (2000).
DOI :
Qute : Komsta, Ł. ,Gowin, E. ,Djordjević Filijović, N. ,Pavlović, A. ,Nikolić, K. ,Agbaba, D. ,Sawant, S. ,Barge, V. ,Tiwari, R. ,Bonde, C. ,Bothara, K. ,Nawaz, M. ,Arayne, M. S. ,Sultana, N. ,Haider, A. ,Hisaindee, S. ,Youssef, R. M. ,Malesevic, M. ,Zivanovic, L. ,Protic, A. ,Radisic, M. ,Lausevic, M. ,Jovic, Z. ,Jóźwiak, A. ,Jóźwiak, G. ,Waksmundzka-Hajnos, M. ,Djapic, N. ,Djapic, N. , Thermodynamic study of urobilinogenic chlorophyll catabolites by reversed-phase liquid chromatography. Acta Chromatographica Vol. 26, no. 1/2014
facebook