Triterpenes in traditional and supercritical-fluid extracts of Morus alba leaf and stem bark

Czasopismo : Acta Chromatographica
Tytuł artykułu : Triterpenes in traditional and supercritical-fluid extracts of Morus alba leaf and stem bark

Autorzy :
Daszykowski, M.
Silesian University Department of Chemometrics, Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Sajewicz, M.
Silesian University Department of General Chemistry and Chromatography 9 Szkolna Street 40-006 Katowice Poland,
Rzepa, J.
Silesian University Department of General Chemistry and Chromatography 9 Szkolna Street 40-006 Katowice Poland,
Hajnos, M.
Medical University of Lublin Department of Pharmacognosy 1 Chodźki Street 20-093 Lublin Poland,
Staszek, D.
Silesian University Department of General Chemistry and Chromatography 9 Szkolna Street 40-006 Katowice Poland,
Wojtal, Ł.
Silesian University Department of General Chemistry and Chromatography 9 Szkolna Street 40-006 Katowice Poland,
Kowalska, T.
Silesian University Department of General Chemistry and Chromatography 9 Szkolna Street 40-006 Katowice Poland,
Waksmundzka-Hajnos, M.
Medical University of Lublin Department of Inorganic Chemistry 6 Staszica Street 20-081 Lublin Poland,
Walczak, B.
Silesian University Department of Chemometrics, Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland, beata@us.edu.pl,
Skalicka-Woźniak, K.
Medical University of Lublin Department of Pharmacognosy with Medicinal Plant Laboratory 1 Chodzki Str. 20-093 Lublin Poland,
Markowski, W.
Medical University of Lublin Department of Physical Chemistry Staszica 6 20-081 Lublin Poland,
Świeboda, R.
Medical University of Lublin Department of Inorganic and Analytical Chemistry Staszica 6 20-081 Lublin Poland,
Głowniak, K.
Medical University of Lublin Department of Pharmacognosy with Medicinal Plant Laboratory 1 Chodzki Str. 20-093 Lublin Poland,
Sultana, N.
University of Karachi Research Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, Faculty of Pharmacy Karachi 75270 Pakistan,
Arayne, M. S.
University of Karachi Department of Chemistry Karachi 75270 Pakistan,
Naveed, S.
University of Karachi Research Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, Faculty of Pharmacy Karachi 75270 Pakistan,
Shamshad, H.
University of Karachi Research Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, Faculty of Pharmacy Karachi 75270 Pakistan,
Li, X.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Xu, J.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Jiang, Y.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Chen, L.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Xu, Y.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Pan, C.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Sheikhloie, H.
slamic Azad University, Science and Research Branch Department of Chemistry, Faculty of Science Poonak-Hesarak, Tehran Iran,
Saber-Tehrani, M.
slamic Azad University, Science and Research Branch Department of Chemistry, Faculty of Science Poonak-Hesarak, Tehran Iran,
Abrumand-Azar, P.
slamic Azad University, Science and Research Branch Department of Chemistry, Faculty of Science Poonak-Hesarak, Tehran Iran,
Waqif-Husain, S.
slamic Azad University, Science and Research Branch Department of Chemistry, Faculty of Science Poonak-Hesarak, Tehran Iran,
Mincea, M.
West University of Timişoara Faculty of Chemistry-Biology-Geography, Department of Chemistry Pestalozzi Street, 16 Timişoara 300115 Romania,
Lupşa, I.
Institute of Public Health Timişoara Department of Food Hygiene V. Babeş 16-18 Timişoara 300226 Romania,
Talpoş, I.
West University of Timişoara Multidisciplinary Research Platform “Nicholas Georgescu-Roegen” Oituz 4 Timişoara Romania,
Ostafe, V.
West University of Timişoara Faculty of Chemistry-Biology-Geography, Department of Chemistry Pestalozzi Street, 16 Timişoara 300115 Romania, vostafe@cbg.uvt.ro,
Milenović, D. M.
Zdravlje-Actavis Company, R&D Analytical Department 16000 Leskovac Serbia, dmilenovic@actavis.rs,
Todorović, Z. B.
University of Niš Faculty of Technology 16000 Leskovac Serbia,
Rane, V. P.
Dr Babasaheb Ambedkar Marathwada University Department of Chemical Technology Aurangabad 431004 (MS) India,
Patil, K. R.
Dr Babasaheb Ambedkar Marathwada University Department of Chemical Technology Aurangabad 431004 (MS) India,
Sangshetti, J. N.
Wockhardt Research Centre Aurangabad 431210 (MS) India,
Yeole, R. D.
Wockhardt Research Centre Aurangabad 431210 (MS) India,
Shinde, D. B.
Dr Babasaheb Ambedkar Marathwada University Department of Chemical Technology Aurangabad 431004 (MS) India,
Ahmad, S.
Hamdard University Department of Pharmaceutics, Faculty of Pharmacy Hamdard Nagar New Delhi 110062 India,
Jain, G. K.
Hamdard University Department of Pharmaceutics, Faculty of Pharmacy Hamdard Nagar New Delhi 110062 India,
Faiyazuddin, M.
Hamdard University Department of Pharmaceutics, Faculty of Pharmacy Hamdard Nagar New Delhi 110062 India,
Iqbal, Z.
Hamdard University Department of Pharmaceutics, Faculty of Pharmacy Hamdard Nagar New Delhi 110062 India,
Talegaonkar, S.
Hamdard University Department of Pharmaceutics, Faculty of Pharmacy Hamdard Nagar New Delhi 110062 India,
Sultana, Y.
Hamdard University Department of Pharmaceutics, Faculty of Pharmacy Hamdard Nagar New Delhi 110062 India,
Ahmad , F. J.
Hamdard University Department of Pharmaceutics, Faculty of Pharmacy Hamdard Nagar New Delhi 110062 India,
Cieśla, Ł.
Medical University of Lublin Department of Inorganic Chemistry 6 Staszica Street 20-081 Lublin Poland,
Skalicka-Woźniak, K.
Medical University of Lublin Department of Pharmacognosy 1 Chodźki Street 20-093 Lublin Poland,
Hajnos, M.
Medical University of Lublin Department of Pharmacognosy 1 Chodźki Street 20-093 Lublin Poland,
Hawrył, M.
Medical University of Lublin Department of Inorganic Chemistry 6 Staszica Street 20-081 Lublin Poland,
Waksmundzka-Hajnos, M.
Medical University of Lublin Department of Inorganic Chemistry 6 Staszica Street 20-081 Lublin Poland,
Böszörményi, A.
Semmelweis University Department of Pharmacognosy Üllői út 26 1085 Budapest Hungary, aboszormenyi@gmail.com,
Szarka, S.
Semmelweis University Department of Pharmacognosy Üllői út 26 1085 Budapest Hungary,
Héthelyi, É.
Eötvös Loránd University Department of Plant Anatomy Pázmány Péter út 1/C 1117 Budapest Hungary,
Gyurján, I.
Budapest University of Technology and Economics Department of Chemical and Environmental Process Engineering Műegyetem rkp. 3. 1111 Budapest Hungary,
László, M.
Eötvös Loránd University Department of Plant Anatomy Pázmány Péter út 1/C 1117 Budapest Hungary,
Simándi, B.
Budapest University of Technology and Economics Department of Chemical and Environmental Process Engineering Műegyetem rkp. 3. 1111 Budapest Hungary,
Szőke, É.
Semmelweis University Department of Pharmacognosy Üllői út 26 1085 Budapest Hungary,
Lemberkovics, É.
Semmelweis University Department of Pharmacognosy Üllői út 26 1085 Budapest Hungary,
Abstrakty : Our objectives were to establish a GC method capable of quantitative analysis of terpenoids without derivatisation and to examine the amount of β -sitosterol extracted from Morus alba L. leaf and stem bark by use of traditional organic solvent extraction and supercritical-fluid extraction (SFE). To measure β -sitosterol content without derivatization, GC-FID was used with 5- α -cholestan-3-one as internal standard. To identify terpenoid constituents, GC-MS was used; β -sitosterol, phytol, lanost-7-en-3-on, α -amyrin, β -amyrin, and lupeol were identified. We established that for Morus leaf the best SFE method for β -sitosterol was pilot scale SFE; the β -sitosterol content of this extract was higher than that of the hexane solvent extract. Among analytical SFE conditions, 200 bar for 90 min and 300 bar for 60 min resulted in extraction of the most β -sitosterol. For mulberry stem bark, solvent extraction with hexane and SFE at 400 bar and 40°C for 60 min proved the best methods.

Słowa kluczowe : Morus alba L, mulberry leaf, stem bark, supercritical fluid extraction, gas chromatography-mass spectrometry, β-sitosterol, α and β-amyrin, lupeol, lanost-7-en-3-one,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2009
Numer : Vol. 21, no. 4
Strony : 659 – 669
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DOI :
Cytuj : Daszykowski, M. ,Sajewicz, M. ,Rzepa, J. ,Hajnos, M. ,Staszek, D. ,Wojtal, Ł. ,Kowalska, T. ,Waksmundzka-Hajnos, M. ,Walczak, B. ,Skalicka-Woźniak, K. ,Markowski, W. ,Świeboda, R. ,Głowniak, K. ,Sultana, N. ,Arayne, M. S. ,Naveed, S. ,Shamshad, H. ,Li, X. ,Xu, J. ,Jiang, Y. ,Chen, L. ,Xu, Y. ,Pan, C. ,Sheikhloie, H. ,Saber-Tehrani, M. ,Abrumand-Azar, P. ,Waqif-Husain, S. ,Mincea, M. ,Lupşa, I. ,Talpoş, I. ,Ostafe, V. ,Milenović, D. M. ,Todorović, Z. B. ,Rane, V. P. ,Patil, K. R. ,Sangshetti, J. N. ,Yeole, R. D. ,Shinde, D. B. ,Ahmad, S. ,Jain, G. K. ,Faiyazuddin, M. ,Iqbal, Z. ,Talegaonkar, S. ,Sultana, Y. ,Ahmad , F. J. ,Cieśla, Ł. ,Skalicka-Woźniak, K. ,Hajnos, M. ,Hawrył, M. ,Waksmundzka-Hajnos, M. ,Böszörményi, A. ,Szarka, S. ,Héthelyi, É. ,Gyurján, I. ,László, M. ,Simándi, B. ,Szőke, É. ,Lemberkovics, É. , Triterpenes in traditional and supercritical-fluid extracts of Morus alba leaf and stem bark. Acta Chromatographica Vol. 21, no. 4/2009
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