Journal : Acta Chromatographica
Article : UPLC-DAD determination of taxodione content in hairy roots of Salvia austriaca Jacq.

Authors :
Kuc, J.
Cracow University of Technology Department of Chemical Engineering and Technology, Department of Analytical Chemistry Warszawska 24 31-155 Cracow Poland, jkuc@chemia.pk.edu.pl,
Grochowalski, A.
Cracow University of Technology Department of Chemical Engineering and Technology, Department of Analytical Chemistry Warszawska 24 31-155 Cracow Poland,
Mach, S.
Institute of Public Health Ostrava Centre of Hygienic Laboratories Dobrá 240 739 51 Frýdek-Místek Czech Republic,
Placha, D.
VŠB-Technical University of Ostrava Centrum Nanotechnology 17 Listopadu 15/2172 708 33 Ostrava Czech Republic,
Furusawa, N.
Osaka City University Graduate School of Human Life Science Osaka 558-8585 Japan,
Stoilova, N.
Bulgarian Food Safety Agency Central Laboratory of Veterinary Control and Ecology (CLVCE) 5 “Iskarsko shousse” str., 1528 Sofia Bulgaria, nadqstoilova@abv.bg,
Surleva, A.
University of Chemical Technology and Metallurgy Analytical Chemistry Department 8 “St. Kl. Ohridski” blvd., 1756 Sofia Bulgaria,
Stoev, G.
Bulgarian Food Safety Agency Central Laboratory of Veterinary Control and Ecology (CLVCE) 5 “Iskarsko shousse” str., 1528 Sofia Bulgaria,
Popovic, N.
Lafayette College Department of Chemistry Easton PA USA,
Sherma, J.
Lafayette College Department of Chemistry Easton PA USA, shermaj@lafayette.edu,
Dixit, S.
Yeungnam University Department of Chemistry Gyeongsan 712-749 South Korea, drdixit.shuchi@gmail.com,
Dubey, R.
riturajdubey0@gmail.com,
Bhushan, R.
Indian Institute of Technology Roorkee Department of Chemistry Roorkee 247667 India, rbushfcy@iitr.ernet.in,
Fouad, M. A.
Cairo University Pharmaceutical Chemistry Department, Faculty of Pharmacy Kasr El-Aini St. Cairo 11562 Egypt, merro911@yahoo.com,
Elkady, E. F.
Cairo University Pharmaceutical Chemistry Department, Faculty of Pharmacy Kasr El-Aini St. Cairo 11562 Egypt,
Csomor, V.
Semmelweis University Department of Pharmacodynamics Budapest Hungary,
Laufer, R.
Semmelweis University Department of Pharmacodynamics Budapest Hungary,
Pöstényi, Z.
Semmelweis University Department of Pharmacology and Pharmacotherapy Budapest Hungary,
Kalász, H.
Semmelweis University Department of Pharmacology and Pharmacotherapy Budapest Hungary, huba.kalasz@gmail.com,
Adeghate, E.
United Arab Emirates University Department of Anatomy Al Ain UAE,
Nurulain, S. M.
United Arab Emirates University Department of Pharmacology and Therapeutics Al Ain UAE,
Tekes, K.
Semmelweis University Department of Pharmacodynamics Budapest Hungary,
Adem, A.
United Arab Emirates University Department of Pharmacology and Therapeutics Al Ain UAE,
Kwiecień, A.
Jagiellonian University Medical College, Faculty of Pharmacy Department of Inorganic and Analytical Chemistry 9 Medyczna Street 30-688 Kraków Poland,
Krzek, J.
Jagiellonian University Medical College, Faculty of Pharmacy Department of Inorganic and Analytical Chemistry 9 Medyczna Street 30-688 Kraków Poland, jankrzek@cm-uj.krakow.pl,
Gądek, M.
Jagiellonian University Medical College, Faculty of Pharmacy Department of Inorganic and Analytical Chemistry 9 Medyczna Street 30-688 Kraków Poland,
Kuźma, Ł.
Medical University of Łódź Department of Biology and Pharmaceutical Botany Muszyńskiego 1 st. 90-151 Łódź Poland, lukasz.kuzma@umed.lodz.p,
Wysokińska, H.
Medical University of Łódź Department of Biology and Pharmaceutical Botany Muszyńskiego 1 st. 90-151 Łódź Poland,
Abstract : Taxodione is the abietane diterpenoid, which has been earlier reported to show cytotoxic, antitumor, antibacterial, and antioxidant properties. This compound has been isolated from a genetically transformed root culture of Salvia austriaca. However, no method for taxodione quantification is yet available. A validated ultra-performance liquid chromatography-diode array detector (UPLC-DAD) method was developed for the quantification of taxodione in a hexane extract taken from genetically transformed roots of S. austriaca. The developed UPLC-DAD method demonstrated good linearity in the range 10–500 μg mL−1 (r2 = 0.99988), high precision (relative standard deviation (RSD) <1.42%), and good repeatability (RSD <1.84%). The limits of detection and quantification of taxodione were found to be 0.48 ng and 1.60 ng, respectively. The recovery of taxodione ranged between 90.4 and 113.1%, and the RSD was less than 2.5%.The developed UPLC-DAD method was then used to determine the content of taxodione in various hairy root lines and changes in amount of the compound during the 50-day growth cycle of the culture. The content of taxodione in hairy root cultures of S. austriaca ranged from 0.29 to 1.12 mg g−1 dry weight depending on root line and stage of growth.
Keywords : taxodione, Salvia austriaca hairy roots, ultra performance liquid chromatography,
Publishing house : University of Silesia in Katowice
Publication date : 2014
Number : Vol. 26, no. 4
Page : 671 – 681

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DOI :
Qute : Kuc, J. ,Grochowalski, A. ,Mach, S. ,Placha, D. ,Furusawa, N. ,Stoilova, N. ,Surleva, A. ,Stoev, G. ,Popovic, N. ,Sherma, J. ,Dixit, S. ,Dubey, R. ,Bhushan, R. ,Fouad, M. A. ,Elkady, E. F. ,Csomor, V. ,Laufer, R. ,Pöstényi, Z. ,Kalász, H. ,Adeghate, E. ,Nurulain, S. M. ,Tekes, K. ,Adem, A. ,Kwiecień, A. ,Krzek, J. ,Gądek, M. ,Kuźma, Ł. ,Wysokińska, H. ,Wysokińska, H. , UPLC-DAD determination of taxodione content in hairy roots of Salvia austriaca Jacq.. Acta Chromatographica Vol. 26, no. 4/2014
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