Validated RP-HPLC method for the simultaneous determination of amlodipine besylate, valsartan, and hydrochlorothiazide in bulk and in pharmaceutical formulation

Czasopismo : Acta Chromatographica
Tytuł artykułu : Validated RP-HPLC method for the simultaneous determination of amlodipine besylate, valsartan, and hydrochlorothiazide in bulk and in pharmaceutical formulation

Autorzy :
Sajewicz, M.
University of Silesia Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Gontarska, M.
University of Silesia Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Kronenbach, D.
University of Silesia Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Berry, E.
University of Silesia Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Kowalska, T.
University of Silesia Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland, teresa.kowalska@us.edu.pl,
Deshpande, P. B.
A.I.S.S.M.S. College of Pharmacy Department of Pharmaceutical Analysis Kennedy Road Pune 411001 MH India, padmanabh77@yahoo.co.in,
Gandhi, S. V.
A.I.S.S.M.S. College of Pharmacy Department of Pharmaceutical Analysis Kennedy Road Pune 411001 MH India,
Gaikwad, N. V.
A.I.S.S.M.S. College of Pharmacy Department of Pharmaceutical Analysis Kennedy Road Pune 411001 MH India,
Khandagle, K. S.
A.I.S.S.M.S. College of Pharmacy Department of Pharmaceutical Analysis Kennedy Road Pune 411001 MH India,
Sateesh, J. N.
PRRM College of Pharmacy Department of Chemistry Kadapa Andhra Pradesh India, nagasateesh@gmail.com,
Subba Reedy, G. V.
JNTUA College of Engineering Department of Chemistry Pulivendula, Y.S.R (Kadapa) Andhra Pradesh India,
Jayaveera, K. N.
awaharlal Nehru Technological University Anantapur Oil Technology Research Institute Anantapur Andhra Pradesh India,
Dhayalamurthi, S.
Mother Theresa Post Graduate & Research Institute of Health Sciences Department of Chemistry, College of Pharmacy Gorimedu Puducherry India,
Anandakumar, K.
Adhiparasakthi College of Pharmacy Department of Pharmaceutical Analysis Melmaruvathur 603 319 Tamil Nadu India, anandkarunakaran@gmail.com,
Jothieswari, D.
Adhiparasakthi College of Pharmacy Department of Pharmaceutical Analysis Melmaruvathur 603 319 Tamil Nadu India,
Subathrai, R.
Adhiparasakthi College of Pharmacy Department of Pharmaceutical Analysis Melmaruvathur 603 319 Tamil Nadu India,
Santhi, D. V.
Adhiparasakthi College of Pharmacy Department of Pharmaceutical Analysis Melmaruvathur 603 319 Tamil Nadu India,
Vetrichelvan, T.
Adhiparasakthi College of Pharmacy Department of Pharmaceutical Analysis Melmaruvathur 603 319 Tamil Nadu India,
Abstrakty : A reverse-phase high-performance liquid chromatographic (RP-HPLC) method was developed for the simultaneous estimation of amlodipine besylate (AMB), valsartan (VAL), and hydrochlorothiazide (HCT) in pharmaceutical formulation using RP-C18 column. The mobile phase (acetonitrile:methanol:50 mM phosphate buffer adjusted to pH 3 with orthophosphoric acid) was pumped at a flow rate of 1.0 mL min-1in the ratio of 20:50:30% v/v and the eluents were monitored at 239 nm. Linearity was obtained in the concentration range of 0.5–5 μg mL-1for AMB, 4–40 μg mL-1for VAL, and 1–10 μg mL-1for HCT. The method was validated as per International Conference on Harmonization (ICH) guidelines and statistically. The method was validated for accuracy and precision. For precision, the coefficient of variance (COV) was found to be 0.3794, 0.1703 and 0.0578, and for accuracy, it was found to be 0.6351, 0.7688 and 1.1305 for AMB, VAL, and HCT, respectively. The COV values for all the drugs were found to be less than 2%, indicating high degree of precision and accuracy of the proposed high-performance liquid chromatographic (HPLC) method. Owing to its simplicity, rapidness, high precision and accuracy, the proposed HPLC method can be applied for determining AMB, VAL, and HCT in bulk and in pharmaceutical dosage form.

Słowa kluczowe : amlodipine besylate, valsartan, hydrochlorothiazide, RP-HPLC,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2012
Numer : Vol. 24, no. 1
Strony : 37 – 50
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DOI :
Cytuj : Sajewicz, M. ,Gontarska, M. ,Kronenbach, D. ,Berry, E. ,Kowalska, T. ,Deshpande, P. B. ,Gandhi, S. V. ,Gaikwad, N. V. ,Khandagle, K. S. ,Sateesh, J. N. ,Subba Reedy, G. V. ,Jayaveera, K. N. ,Dhayalamurthi, S. ,Anandakumar, K. ,Jothieswari, D. ,Subathrai, R. ,Santhi, D. V. ,Vetrichelvan, T. , Validated RP-HPLC method for the simultaneous determination of amlodipine besylate, valsartan, and hydrochlorothiazide in bulk and in pharmaceutical formulation. Acta Chromatographica Vol. 24, no. 1/2012
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