Category: Vol. 25, no. 2

HPTLC/HPLC and gravimetric methodology for the identification and quantification of gymnemic acid from Gymnema sylvestre methanolic extracts

Czasopismo : Acta Chromatographica
Tytuł artykułu : HPTLC/HPLC and gravimetric methodology for the identification and quantification of gymnemic acid from Gymnema sylvestre methanolic extracts

Autorzy :
Huo, H. L.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Yu, S. H.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Liu, X. Z.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Meng, Y.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Ren, Y. P.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Zhang, L. T.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China, zhanglantong@263.net,
Liu, L.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Zhao, T.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Cheng, X. M.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Wang, C. H.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China, wchcxm@hotmail.com,
Wang, Z. T.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China, wangzt@hotmail.com,
Gao, X.
Sichuan University Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy Chengdu, Sichuan China, gaomuxouzi@126.com,
Jiang, X.
Sichuan University Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy Chengdu, Sichuan China,
Wang, L.
Sichuan University Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy Chengdu, Sichuan China,
Urban, J.
University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, School of Complex Systems (former Institute of Physical Biology), Laboratory of Applied System Biology Zamek 136 Nove Hrady 373 33 Czech Republic, urbanj@frov.jcu.cz,
Vaněk, J.
University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, School of Complex Systems (former Institute of Physical Biology), Laboratory of Applied System Biology Zamek 136 Nove Hrady 373 33 Czech Republic,
Štys, D.
University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, School of Complex Systems (former Institute of Physical Biology), Laboratory of Applied System Biology Zamek 136 Nove Hrady 373 33 Czech Republic,
Li, C. D.
Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P.R. China,
Liu, Y.
Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P.R. China,
Shi, Y. P.
Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P.R. China, shiyp@licp.cas.cn,
Zhang, Y.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China, hangsong@vip.sina.com,
Peng, Q.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Zeng, H.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Yao, S.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Zhang, Y.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Song, H.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Zhou, W.
Guiyang Medical University Department of Pharmaceutical Analysis, School of Pharmacy Guiyang 550004 China,
Zhang, X. Y.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China, drxyzhang@126.com,
Lv, Y. P.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China,
Liu, X. D.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China,
Xu, C.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China,
Duan, G. L.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China, glduan@shmu.edu.cn,
Smoliński, A.
Central Mining Institute Department of Energy Saving and Air Protection Sq. Gwarkow 1 40-166 Katowice, Poland, smolin@gig.katowice.pl,
Howaniec, N.
Central Mining Institute Department of Energy Saving and Air Protection Sq. Gwarkow 1 40-166 Katowice, Poland,
Unver-Somer, N.
Ege University Faculty of Pharmacy, Department of Pharmacognosy 35100 Bornova, Izmir Turkey,
Cicek-Polat, D.
Ege University Faculty of Pharmacy, Department of Pharmacognosy 35100 Bornova, Izmir Turkey,
Emir, A.
Ege University Faculty of Pharmacy, Department of Pharmacognosy 35100 Bornova, Izmir Turkey,
Onur, M. A.
Ege University Faculty of Pharmacy, Department of Pharmacognosy 35100 Bornova, Izmir Turkey,
Kaya, G. I.
Ege University Faculty of Pharmacy, Department of Pharmacognosy 35100 Bornova, Izmir Turkey,
Ahmed, A. B. A.
Bharathidasan University Department of Plant Science Tiruchirappalli 620 024 Tamil Nadu India, dr.bakrudeenaliahmed@yahoo.co.in,
Rao, A. S.
Bharathidasan University Department of Biotechnology Tiruchirappalli 620 024 Tamil Nadu India,
Rao, M. V.
Bharathidasan University Department of Plant Science Tiruchirappalli 620 024 Tamil Nadu India,
Taha, R. M.
University of Malaya Institute of Biological Sciences, Faculty of Science 50603 Kuala Lumpur Malaysia,
Abstrakty : Gymnemic acid (GA), a well known anti-diabetic compound has been detected in methanol extracts of intact leaves and in vitro callus cultures derived from leaf explants of Gymnema sylvestre. Callus biomass was developed in MS medium with optimum plant growth regulators (OPGRs) of 2,4-D (1.5 mg L-1) + KN (0.5 mg L-1) under abiotic stress conditions at 45 days determined by growth curve analysis. GA detection and quantification were carried out using thin-layer chromatography (TLC), highperformance thin-layer chromatography (HPTLC), high-performance liquid chromatography (HPLC), and gravimetric techniques. GA detection peak area and their absorption spectra were evaluated through HPTLC and HPLC with the standard GA. Quantification of GA had showed the linearity, accuracy, robustness and precision by HPLC. GA content was significantly higher in gravimetric method than HPLC. All these methods were found to be simple, accurate, selective and rapid and could be successfully applied for the determination of GA. It could have potential as a pharmaceutical drug for Type 1 diabetes mellitus (IDDM) and obesity.

Słowa kluczowe : Gymnema sylvestre, gymnemic acid (GA), abiotic stress, HPTLC, HPLC, gravimetric method,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2013
Numer : Vol. 25, no. 2
Strony : 339 – 361
Bibliografia : 1 International Expert Committee, Report on the role of the A1C assay in the diagnosis of diabetes, Diabetes Care, 32, 1–8 (2009)
2 WHO (2010), http://www.who.int/features/factfiles/obesity/en/
3 S.K. Mitra, S. Gopumadhavan, T.S. Muralidhar, S.D. Anturlikar, and M.B. Sujatha, Indian J. Exp. Biol., 33, 798 (1995)
4 K.R. Shanmugasundaram, C. Panneerselvam, P. Samudram, and E.R.B. Shanmugasundaram, J. Ethnopharmacol., 7, 205 (1983)
5 E.R.B. Shanmugasundaram, K.G. Leela, K.S. Radha, and V.M. Rajendran, J. Ethnopharmacol., 30, 265 (1990)
6 Y. Nakamura, Y. Tsumura, Y. Tonogai, and T. Shibata, J. Nutr., 129, 1214 (1999)
7 W.C. Ye, Q. Zhang, X. Liu, X. Che, and S. Zhao, Phytochemistry, 53, 893 (2000)
8 A. Sokmen, B.M. Jones, and M. Erturk, Phytother. Res. 13, 355 (1999)
9 G. Hellekant, D.E. Walters, In: J.A.K Mathlouthi, G.G. Birch (eds), Sweet-Taste Chemoreception, Elsevier, London, 1991, p. 373
10 P.V. Kanetkar, R.S. Singhal, K.S. Laddha, and M.Y. Kamat, Phytochem. Anal., 17, 409 (2006)
11 C.S. Devi, S. Murugesh, and V.M. Srinivasan, J. Appl. Sci., 6, 2263 (2006)
12 E.J. Lee, M. Mobin, E.J. Hahn, and K.Y. Peak, J. Plant Biotech., 49, 427 (2006)
13 A.B. Ali Ahmed, A.S. Rao, and M.V. Rao, Methods Mol. Biol., 547, 93 (2009)
14 A.B.A. Ahmed, A.S. Rao, M.V. Rao, and R.M. Taha ScientificWorldJournal., 897867 (2012)
15 A.B.A. Ahmed, A.S. Rao, and M.V. Rao, Biomedicine, 28, 134 (2008)
16 A.B.A. Ahmed, A.S. Rao, and M.V. Rao, Phytomedicine, 17, 1033 (2010)
17 T. Murashige and F. Skoog, Physiol. Plant, 15, 473 (1962)
18 R.U. Rehman, M. Israr, P.S. Srivastava, K.C. Bansal, and M.Z. Abdin, In Vitro Cell Dev. Biol. Plant, 39, 142 (2003)
19 V. Rajpal, Standardization of Botanicals-testing and extraction methods of medicinal herbs, Eastern Publishers, New Delhi, 2002, p. 140
20 G. Shin, M. Chil, and D. Lee, Biol. Pharm. Bull., 26, 1321 (2003)
21 Smetanska, Adv. Biochem. Eng. Biotech., 111, 197 (2008)
22 Y.C. Wang, H.X. Zhang, B. Zhao, and X.F. Yuan, Biotech. Lett., 23, 1971 (2001)
23 A. Marston, M. Maillard, and K. Hostettmann, GIT Laboratory J., 1, 36 (1997)
24 S.I. Johannessen, D. Battino, D.J. Berry, M. Bialer, G. Kramer, T. Tomson, and P.N. Patsalo, Ther. Drug. Monit., 25, 347 (2003)
25 V.S.R. Raju, S. Kannababu, and G.V. Subbraju, Phytochemical Anal., 17, 192 (2006)
26 A.B.A. Ahmed, N. Komalavalli, M. Muthukumar, J.H.F. Benjamin, A.S. Rao, S.K. Kim, and M.V. Rao, Comprehensive Bioactive Natural Products-Potential and Challenges, 1, 2009, p. 75
27 T. Imoto, F. Yamamoto, A. Miyasaka, and H. Hatano, J. Chromatogr., 557, 383 (1991)
28 K. Yoshikawa, K. Amimoto, S. Arihara, and K. Matsuura, Tetrahedron Lett. 30, 1103 (1989)
29 Y. Sugihara, H. Nojima, H. Matsuda, T. Murakami, M. Yoshikawa, and I. Kimura, J. Asian Nat. Prod. Res., 2, 321 (2000)
30 K. Hostettmann, J.L. Wolfender, and S. Rodriguez, Planta Med., 63, 2 (1997)
31 C.S. Joshi, E.S. Priya, and S. Venkataraman, J. Health Sci., 53, 245 (2007)
32 A. Bakrudeen Ali Ahmed, A.S. Rao, M.V. Rao, and R.M. Taha, Agro Food Ind Hi Tec., 23, 34 (2012)
DOI :
Cytuj : Huo, H. L. ,Yu, S. H. ,Liu, X. Z. ,Meng, Y. ,Ren, Y. P. ,Zhang, L. T. ,Liu, L. ,Zhao, T. ,Cheng, X. M. ,Wang, C. H. ,Wang, Z. T. ,Gao, X. ,Jiang, X. ,Wang, L. ,Urban, J. ,Vaněk, J. ,Štys, D. ,Li, C. D. ,Liu, Y. ,Shi, Y. P. ,Zhang, Y. ,Peng, Q. ,Zeng, H. ,Yao, S. ,Zhang, Y. ,Song, H. ,Zhou, W. ,Zhang, X. Y. ,Lv, Y. P. ,Liu, X. D. ,Xu, C. ,Duan, G. L. ,Smoliński, A. ,Howaniec, N. ,Unver-Somer, N. ,Cicek-Polat, D. ,Emir, A. ,Onur, M. A. ,Kaya, G. I. ,Ahmed, A. B. A. ,Rao, A. S. ,Rao, M. V. ,Taha, R. M. , HPTLC/HPLC and gravimetric methodology for the identification and quantification of gymnemic acid from Gymnema sylvestre methanolic extracts. Acta Chromatographica Vol. 25, no. 2/2013

Micro-sample analysis of capsaicin by liquid chromatography-tandem mass spectrometry assay and application to oral absorption study

Czasopismo : Acta Chromatographica
Tytuł artykułu : Micro-sample analysis of capsaicin by liquid chromatography-tandem mass spectrometry assay and application to oral absorption study

Autorzy :
Huo, H. L.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Yu, S. H.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Liu, X. Z.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Meng, Y.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Ren, Y. P.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Zhang, L. T.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China, zhanglantong@263.net,
Liu, L.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Zhao, T.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Cheng, X. M.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Wang, C. H.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China, wchcxm@hotmail.com,
Wang, Z. T.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China, wangzt@hotmail.com,
Gao, X.
Sichuan University Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy Chengdu, Sichuan China, gaomuxouzi@126.com,
Jiang, X.
Sichuan University Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy Chengdu, Sichuan China,
Wang, L.
Sichuan University Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy Chengdu, Sichuan China,
Urban, J.
University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, School of Complex Systems (former Institute of Physical Biology), Laboratory of Applied System Biology Zamek 136 Nove Hrady 373 33 Czech Republic, urbanj@frov.jcu.cz,
Vaněk, J.
University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, School of Complex Systems (former Institute of Physical Biology), Laboratory of Applied System Biology Zamek 136 Nove Hrady 373 33 Czech Republic,
Štys, D.
University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, School of Complex Systems (former Institute of Physical Biology), Laboratory of Applied System Biology Zamek 136 Nove Hrady 373 33 Czech Republic,
Li, C. D.
Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P.R. China,
Liu, Y.
Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P.R. China,
Shi, Y. P.
Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P.R. China, shiyp@licp.cas.cn,
Zhang, Y.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China, hangsong@vip.sina.com,
Peng, Q.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Zeng, H.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Yao, S.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Zhang, Y.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Song, H.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Zhou, W.
Guiyang Medical University Department of Pharmaceutical Analysis, School of Pharmacy Guiyang 550004 China,
Zhang, X. Y.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China, drxyzhang@126.com,
Lv, Y. P.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China,
Liu, X. D.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China,
Xu, C.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China,
Duan, G. L.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China, glduan@shmu.edu.cn,
Smoliński, A.
Central Mining Institute Department of Energy Saving and Air Protection Sq. Gwarkow 1 40-166 Katowice, Poland, smolin@gig.katowice.pl,
Howaniec, N.
Central Mining Institute Department of Energy Saving and Air Protection Sq. Gwarkow 1 40-166 Katowice, Poland,
Unver-Somer, N.
Ege University Faculty of Pharmacy, Department of Pharmacognosy 35100 Bornova, Izmir Turkey,
Cicek-Polat, D.
Ege University Faculty of Pharmacy, Department of Pharmacognosy 35100 Bornova, Izmir Turkey,
Emir, A.
Ege University Faculty of Pharmacy, Department of Pharmacognosy 35100 Bornova, Izmir Turkey,
Onur, M. A.
Ege University Faculty of Pharmacy, Department of Pharmacognosy 35100 Bornova, Izmir Turkey,
Kaya, G. I.
Ege University Faculty of Pharmacy, Department of Pharmacognosy 35100 Bornova, Izmir Turkey,
Ahmed, A. B. A.
Bharathidasan University Department of Plant Science Tiruchirappalli 620 024 Tamil Nadu India, dr.bakrudeenaliahmed@yahoo.co.in,
Rao, A. S.
Bharathidasan University Department of Biotechnology Tiruchirappalli 620 024 Tamil Nadu India,
Rao, M. V.
Bharathidasan University Department of Plant Science Tiruchirappalli 620 024 Tamil Nadu India,
Taha, R. M.
University of Malaya Institute of Biological Sciences, Faculty of Science 50603 Kuala Lumpur Malaysia,
Choi, Y.
Sungkyunkwan University School of Pharmacy Suwon, Gyeonggi-do Korea,
Shin, B. S.
Catholic University of Daegu College of Pharmacy Gyeongsan-si, Gyeongbuk Korea,
Kim, M. G.
Sungkyunkwan University School of Pharmacy Suwon, Gyeonggi-do Korea,
Shin, S. W.
Sungkyunkwan University School of Pharmacy Suwon, Gyeonggi-do Korea,
Kim, T. H.
Catholic University of Daegu College of Pharmacy Gyeongsan-si, Gyeongbuk Korea,
Jang, H. C.
Sungkyunkwan University School of Pharmacy Suwon, Gyeonggi-do Korea,
Yoo, S. D.
Sungkyunkwan University School of Pharmacy Suwon, Gyeonggi-do Korea, sdyoo@skku.ac.kr,
Abstrakty : Capsaicin has been reported to exhibit an inhibitory effect on the P-glycoprotein (P-gp) function in vitro. To investigate its concentration-dependent effect in vivo, a sensitive assay that can characterize the absorption and disposition of capsaicin needs to be developed. This study reports the development of a sensitive LC-MS/MS assay for the determination of capsaicin in mouse plasma. The sample pretreatment involved a one-step extraction of 20 μL plasma with t-butyl methyl ether. Separations were achieved on a C18 column and the detection was performed on an LC-ESI-MS/MS by multiple reaction monitoring. The assay was linear over a wide concentration range from 0.325 to 650 ng mL-1(r > 0.999), with a LLOQ of 0.325 ng mL-1. The developed method was applied to i.v. (dose 0.325 and 0.65 mg kg) and oral absorption (dose 40 mg kg) studies in mice. After i.v. injection, the t1/2,λz, Vz and CLs ranged from 0.13–0.16 h, 127.6–141.8 mL, and 547.3–775.4 mL/h, respectively. After oral administration, a secondary peak was observed and the terminal half-life was prolonged (1.51 h). Capsaicin was poorly absorbed, with the absolute oral bioavailability (F) ranging from 1.02% to 1.56%. The developed assay may be useful in studies where sample volumes are limited.

Słowa kluczowe : capsaicin, LC-MS/MS, bioavailability, pharmacokinetics,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2013
Numer : Vol. 25, no. 2
Strony : 363 – 376
Bibliografia : 1 G.A. Cordell and O.E. Araujo, Ann. Pharmacother., 27, 330 (1993)
2 M.J. Caterina, M.A. Schumacher, M. Tominaga, T.A. Rosen, J.D. Levine, and D. Julius, Nature, 389, 816 (1997)
3 I. Tolan, D. Ragoobirsingh and E.Y.S.A. Morrison, Phytother. Res., 15, 391 (2001)
4 C.C. Chou, Y.C. Wu, Y.F. Wang, M.J. Chou, S.J. Kuo, and D.R. Chen, Oncol. Rep., 21, 665 (2009)
5 S.P. Huang, J.C. Chen, C.C. Wu, C.T. Chen, N.Y. Tang, Y.T. Ho, C. Lo, J.P. Lin, J.G. Chung, and J.G. Lin, Anticancer Res., 29, 165 (2009)
6 M.Y. Kim, L.J. Trudel, and G.N. Wogan, Anticancer Res., 29, 3733 (2009)
7 K.C. Brown, T.R. Witte, W.E. Hardman, H. Luo, Y.C. Chen, A.B. Carpenter, J.K. Lau, and P. Dasgupta, PLos ONE, 5, 1 (2010)
8 T. Nabekura, S. Kamiyama, and S. Kitagawa, Biochem. Biophys. Res Commun., 327, 866 (2005)
9 O.M.E.A. Salam, O.A. Heikal, and S. El-Shenawy, Pharmacology, 73, 121 (2005)
10 D. Suresh and K. Srivasan, Indian J. Med. Res., 131, 682 (2010)
11 J. Lu and M. Cwik, J. Chromatogr. B, 701, 135 (1997)
12 R. Wolf, C. Huschka, K. Raith, W. Wohlrab, and R.H.H. Neubert, J. Liquid Chromatogr. Rel. Technol., 22, 531 (1999)
13 C.A. Reilly, D.J. Crouch, G.S. Yost, and A.A. Fatah, J. Anal. Toxicol., 26, 313 (2002)
14 S. Babbar, J.F. Marier, M.S. Moukassi, M. Beliveau, G.F. Vanhove, S. Chanda, and K. Bley, Ther. Drug Monit., 31, 502 (2009)
15 Q. Zhang, J. Hu, L. Sheng, and Y. Li, J. Chromatogr. B Anal. Technol. Biomed. Life Sci., 878, 2292 (2010)
16 S. Chanda, M. Bashir, S. Babbar, A. Koganti, and K. Bley, Drug Metab. Dispos., 36, 670 (2008)
17 B.S. Shin, S.W. Hwang, J.B. Bulitta, J.B. Lee, S.D. Yang, J.S. Park, M.C. Kwon, D.J. Kim, H.S. Yoon, and S.D. Yoo, J. Toxicol. Environ. Health-Part A, 73, 1586 (2010)
DOI :
Cytuj : Huo, H. L. ,Yu, S. H. ,Liu, X. Z. ,Meng, Y. ,Ren, Y. P. ,Zhang, L. T. ,Liu, L. ,Zhao, T. ,Cheng, X. M. ,Wang, C. H. ,Wang, Z. T. ,Gao, X. ,Jiang, X. ,Wang, L. ,Urban, J. ,Vaněk, J. ,Štys, D. ,Li, C. D. ,Liu, Y. ,Shi, Y. P. ,Zhang, Y. ,Peng, Q. ,Zeng, H. ,Yao, S. ,Zhang, Y. ,Song, H. ,Zhou, W. ,Zhang, X. Y. ,Lv, Y. P. ,Liu, X. D. ,Xu, C. ,Duan, G. L. ,Smoliński, A. ,Howaniec, N. ,Unver-Somer, N. ,Cicek-Polat, D. ,Emir, A. ,Onur, M. A. ,Kaya, G. I. ,Ahmed, A. B. A. ,Rao, A. S. ,Rao, M. V. ,Taha, R. M. ,Choi, Y. ,Shin, B. S. ,Kim, M. G. ,Shin, S. W. ,Kim, T. H. ,Jang, H. C. ,Yoo, S. D. , Micro-sample analysis of capsaicin by liquid chromatography-tandem mass spectrometry assay and application to oral absorption study. Acta Chromatographica Vol. 25, no. 2/2013
[Top]

Separation and analysis of phenylethanoid glycosides in Lamiophlomis rotata by high-performance liquid chromatography

Czasopismo : Acta Chromatographica
Tytuł artykułu : Separation and analysis of phenylethanoid glycosides in Lamiophlomis rotata by high-performance liquid chromatography

Autorzy :
Huo, H. L.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Yu, S. H.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Liu, X. Z.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Meng, Y.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Ren, Y. P.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Zhang, L. T.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China, zhanglantong@263.net,
Liu, L.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Zhao, T.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Cheng, X. M.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Wang, C. H.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China, wchcxm@hotmail.com,
Wang, Z. T.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China, wangzt@hotmail.com,
Gao, X.
Sichuan University Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy Chengdu, Sichuan China, gaomuxouzi@126.com,
Jiang, X.
Sichuan University Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy Chengdu, Sichuan China,
Wang, L.
Sichuan University Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy Chengdu, Sichuan China,
Urban, J.
University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, School of Complex Systems (former Institute of Physical Biology), Laboratory of Applied System Biology Zamek 136 Nove Hrady 373 33 Czech Republic, urbanj@frov.jcu.cz,
Vaněk, J.
University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, School of Complex Systems (former Institute of Physical Biology), Laboratory of Applied System Biology Zamek 136 Nove Hrady 373 33 Czech Republic,
Štys, D.
University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, School of Complex Systems (former Institute of Physical Biology), Laboratory of Applied System Biology Zamek 136 Nove Hrady 373 33 Czech Republic,
Li, C. D.
Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P.R. China,
Liu, Y.
Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P.R. China,
Shi, Y. P.
Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P.R. China, shiyp@licp.cas.cn,
Zhang, Y.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China, hangsong@vip.sina.com,
Peng, Q.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Zeng, H.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Yao, S.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Zhang, Y.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Song, H.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Zhou, W.
Guiyang Medical University Department of Pharmaceutical Analysis, School of Pharmacy Guiyang 550004 China,
Zhang, X. Y.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China, drxyzhang@126.com,
Lv, Y. P.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China,
Liu, X. D.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China,
Xu, C.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China,
Duan, G. L.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China, glduan@shmu.edu.cn,
Smoliński, A.
Central Mining Institute Department of Energy Saving and Air Protection Sq. Gwarkow 1 40-166 Katowice, Poland, smolin@gig.katowice.pl,
Howaniec, N.
Central Mining Institute Department of Energy Saving and Air Protection Sq. Gwarkow 1 40-166 Katowice, Poland,
Unver-Somer, N.
Ege University Faculty of Pharmacy, Department of Pharmacognosy 35100 Bornova, Izmir Turkey,
Cicek-Polat, D.
Ege University Faculty of Pharmacy, Department of Pharmacognosy 35100 Bornova, Izmir Turkey,
Emir, A.
Ege University Faculty of Pharmacy, Department of Pharmacognosy 35100 Bornova, Izmir Turkey,
Onur, M. A.
Ege University Faculty of Pharmacy, Department of Pharmacognosy 35100 Bornova, Izmir Turkey,
Kaya, G. I.
Ege University Faculty of Pharmacy, Department of Pharmacognosy 35100 Bornova, Izmir Turkey,
Ahmed, A. B. A.
Bharathidasan University Department of Plant Science Tiruchirappalli 620 024 Tamil Nadu India, dr.bakrudeenaliahmed@yahoo.co.in,
Rao, A. S.
Bharathidasan University Department of Biotechnology Tiruchirappalli 620 024 Tamil Nadu India,
Rao, M. V.
Bharathidasan University Department of Plant Science Tiruchirappalli 620 024 Tamil Nadu India,
Taha, R. M.
University of Malaya Institute of Biological Sciences, Faculty of Science 50603 Kuala Lumpur Malaysia,
Choi, Y.
Sungkyunkwan University School of Pharmacy Suwon, Gyeonggi-do Korea,
Shin, B. S.
Catholic University of Daegu College of Pharmacy Gyeongsan-si, Gyeongbuk Korea,
Kim, M. G.
Sungkyunkwan University School of Pharmacy Suwon, Gyeonggi-do Korea,
Shin, S. W.
Sungkyunkwan University School of Pharmacy Suwon, Gyeonggi-do Korea,
Kim, T. H.
Catholic University of Daegu College of Pharmacy Gyeongsan-si, Gyeongbuk Korea,
Jang, H. C.
Sungkyunkwan University School of Pharmacy Suwon, Gyeonggi-do Korea,
Yoo, S. D.
Sungkyunkwan University School of Pharmacy Suwon, Gyeonggi-do Korea, sdyoo@skku.ac.kr,
Dong, S.
The Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 China, sqdong@licp.cas.cn,
Shi, X.
Gansu Academy of Medical Sciences Lanzhou 730000 China,
Liu, Q.
Graduate University of the Chinese Academy of Sciences Beijing 100039 China,
Zhang, Z.
The Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 China,
Zhao, L.
The Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 China,
Abstrakty : A simple hydrolysis method has been developed for determination of phenylethanoid glycosides in Lamiophlomis rotata (L.R.). Different kinds of phenylethanoid glycosides were hydrolyzed in hydrochloric acid solution to produce corresponding phenethyl alcohols and cinnamic acids, mainly containing hydroxytyrosol, homovanillyl alcohol, 3,4-dimethoxyphenethyl alcohol, caffeic acid, fumalic acid and 3,4-dimethoxycinnamic acid. The six analytes could be determined simultaneously by high-performance liquid chromatography (HPLC). The effects of mobile phase, pH and concentration of running buffer, detection wavelength, flow rate and injection volume were also investigated. Under the optimum conditions, the six hydrolyzates could be perfectly separated within 45 min. The response was linear over four orders of magnitude with detection limits (S/N = 3) ranging from 1 × 10-8to 1.5 × 10-4mol L-1for the analytes. The method has been successfully applied to the analysis of real sample Du-Yi-Wei capsule and Qi-Zheng-Yan-Tong patch, with satisfactory results.

Słowa kluczowe : Lamiophlomis rotata (Benth) Kudo, high-performance liquid chromatography, phenylethanoid glycosides, hydrolyzation,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2013
Numer : Vol. 25, no. 2
Strony : 377 – 392
Bibliografia : 1 M.X. Li, R.X. Zhang, Z.P. Jia, J. Sheng, J.G. Qiu, and J. Wang, Phytother. Res., 23, 816 (2009)
2 M.X. Li, Z.P. Jia, and R.X. Zhang, J. Chin. Med. Mater., 3, 222 (2004)
3 J.H. Yi, X.Z. Yan, Z.Y. Luo, and Z.C. Zhong, Acta Pharm. Sin., 30, 206 (1995)
4 D.R. Wang, L.N. Sun, Z.Y. Tao, W.D. Zhang, and W.S. Chen, J. Sec. Mil. Med. Univ., 10, 1171 (2005)
5 M.X. Li, Z.P. Jia, R. Zhang, Z.D. Hu, and X. Tian, Carbohydr. Res., 343, 561 (2008)
6 S.P. Wang and K.J. Huang, J. Chromatogr. A, 1032, 273 (2004)
7 S.A. Wang, D. Di, X. Liu, and S. Jiang, J. Liq. Chromatogr. Relat. Technol., 30, 1991 (2007)
8 K. Ishii, T. Furuta, and Y. Kasuya, J. Chromatogr. B, 794, 49 (2003)
9 S. de Pascual-Teresa, D. Treutter, J.C. Rivas-Gonzalo, and C. Santos-Buelga, J. Agric. Food Chem., 46, 4209 (1998)
10 T. Akiyama, T. Yamada, and T. Maitani, J. Chromatogr. A, 895, 279 (2000)
11 T. Bo, K.A. Li, and H. Liu, Anal. Chim. Acta, 458, 345 (2002)
12 X.Q. Xu, H.Z. Ye, W. Wang, and G.N. Chen, J. Agric. Food Chem., 53, 5853 (2005)
13 P. Morin, F. Villard, and M. Dreux, J. Chromatogr. A, 628, 153 (1993)
14 P. Morin, F. Villard, and M. Dreux, J. Chromatogr. A, 628, 161 (1993)
15 L.Z. Lin, X.G. He, M. Lindenmaier, J. Yang, M. Clerary, S.X. Qiu, and G.A. Cordell, J. Agric. Food Chem., 48, 354 (2000)
16 W. Wu, C.Y. Yan, L. Li, Z.Q. Liu, and S.Y. Liu, J. Chromatogr. A, 1047, 213 (2004)
17 F. Sánchez-Rabaneda, O. Jáuregui, R.M. Lamuela-Raventós, J. Bastida, F. Viladomat, and C. Codina, J. Chromatogr. A, 1008, 57 (2003)
18 Y.C. Fiamegos, C.G. Nanos, J. Vervoort, and C.D. Stalikas, J. Chromatogr. A, 1041, 11 (2004)
19 F.X. Deng and S.W. Zito, J. Chromatogr. A, 986, 121 (2003)
20 C.Z. Zhang, C. Li, and S.L. Feng, Phytochemistry, 12, 4156 (1991)
21 F. Zhang, L.N Sun, and W.S. Chen, Chem. Nat. Comp., 45, 360 (2009)
22 M.X. Li, R.X. Zhang, C.X. Li, P.C. Fan, Q.L. Zhang, and Z.P. Jia, Anal. Methods, 2, 714 (2010)
23 X.K. Wang and L. Xu, Thromb. Res., 115, 95 (2005)
24 M.X. Lia, X.F. Shang, R.X. Zhang, Z.P. Jia, P.C. Fan, Q. Ying, and L.L. Wei, Fitoterapia, 81, 167 (2010)
25 M.N. Luo, H.W. Lu, H.Q. Ma, L. Zhao, X. Liu, and S.X. Jiang, J. Pharm. Biomed. Anal. 44, 881 (2007)
26 A. Pieroni, P. Pachaly, Y. Huang, B. Vanpoel, and A.J. Vlietinck, J. Ethnopharmacol., 70, 213 (2000)
27 N.E. Es-Safia, A. Kollmann, S. Khlific, and P.H. Ducrot, LWT, 40, 1246 (2007)
28 J. Han, M. Ye, H. Guo, M. Yang, B. Wang, and D. Guo, J. Pharm. Biomed. Anal., 44, 430 (2007)
29 A. Karioti, L. Bolognesi, F.F. Vincieri, and A.R. Bilia, J. Pharm. Biomed. Anal., 53, 15 (2010)
30 S.Q. Dong, L.Z. Chi, P.G. He, Q.J. Wang, and Y.Z. Fang, Talanta, 80, 809 (2009)
DOI :
Cytuj : Huo, H. L. ,Yu, S. H. ,Liu, X. Z. ,Meng, Y. ,Ren, Y. P. ,Zhang, L. T. ,Liu, L. ,Zhao, T. ,Cheng, X. M. ,Wang, C. H. ,Wang, Z. T. ,Gao, X. ,Jiang, X. ,Wang, L. ,Urban, J. ,Vaněk, J. ,Štys, D. ,Li, C. D. ,Liu, Y. ,Shi, Y. P. ,Zhang, Y. ,Peng, Q. ,Zeng, H. ,Yao, S. ,Zhang, Y. ,Song, H. ,Zhou, W. ,Zhang, X. Y. ,Lv, Y. P. ,Liu, X. D. ,Xu, C. ,Duan, G. L. ,Smoliński, A. ,Howaniec, N. ,Unver-Somer, N. ,Cicek-Polat, D. ,Emir, A. ,Onur, M. A. ,Kaya, G. I. ,Ahmed, A. B. A. ,Rao, A. S. ,Rao, M. V. ,Taha, R. M. ,Choi, Y. ,Shin, B. S. ,Kim, M. G. ,Shin, S. W. ,Kim, T. H. ,Jang, H. C. ,Yoo, S. D. ,Dong, S. ,Shi, X. ,Liu, Q. ,Zhang, Z. ,Zhao, L. , Separation and analysis of phenylethanoid glycosides in Lamiophlomis rotata by high-performance liquid chromatography. Acta Chromatographica Vol. 25, no. 2/2013
[Top]

A validated stability-indicating RP-LC method for the estimation of process-related impurities and degradation products of quetiapine fumarate in solid oral dosage form

Czasopismo : Acta Chromatographica
Tytuł artykułu : A validated stability-indicating RP-LC method for the estimation of process-related impurities and degradation products of quetiapine fumarate in solid oral dosage form

Autorzy :
Huo, H. L.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Yu, S. H.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Liu, X. Z.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Meng, Y.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Ren, Y. P.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Zhang, L. T.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China, zhanglantong@263.net,
Liu, L.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Zhao, T.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Cheng, X. M.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Wang, C. H.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China, wchcxm@hotmail.com,
Wang, Z. T.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China, wangzt@hotmail.com,
Gao, X.
Sichuan University Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy Chengdu, Sichuan China, gaomuxouzi@126.com,
Jiang, X.
Sichuan University Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy Chengdu, Sichuan China,
Wang, L.
Sichuan University Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy Chengdu, Sichuan China,
Urban, J.
University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, School of Complex Systems (former Institute of Physical Biology), Laboratory of Applied System Biology Zamek 136 Nove Hrady 373 33 Czech Republic, urbanj@frov.jcu.cz,
Vaněk, J.
University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, School of Complex Systems (former Institute of Physical Biology), Laboratory of Applied System Biology Zamek 136 Nove Hrady 373 33 Czech Republic,
Štys, D.
University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, School of Complex Systems (former Institute of Physical Biology), Laboratory of Applied System Biology Zamek 136 Nove Hrady 373 33 Czech Republic,
Li, C. D.
Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P.R. China,
Liu, Y.
Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P.R. China,
Shi, Y. P.
Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P.R. China, shiyp@licp.cas.cn,
Zhang, Y.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China, hangsong@vip.sina.com,
Peng, Q.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Zeng, H.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Yao, S.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Zhang, Y.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Song, H.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Zhou, W.
Guiyang Medical University Department of Pharmaceutical Analysis, School of Pharmacy Guiyang 550004 China,
Zhang, X. Y.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China, drxyzhang@126.com,
Lv, Y. P.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China,
Liu, X. D.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China,
Xu, C.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China,
Duan, G. L.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China, glduan@shmu.edu.cn,
Smoliński, A.
Central Mining Institute Department of Energy Saving and Air Protection Sq. Gwarkow 1 40-166 Katowice, Poland, smolin@gig.katowice.pl,
Howaniec, N.
Central Mining Institute Department of Energy Saving and Air Protection Sq. Gwarkow 1 40-166 Katowice, Poland,
Unver-Somer, N.
Ege University Faculty of Pharmacy, Department of Pharmacognosy 35100 Bornova, Izmir Turkey,
Cicek-Polat, D.
Ege University Faculty of Pharmacy, Department of Pharmacognosy 35100 Bornova, Izmir Turkey,
Emir, A.
Ege University Faculty of Pharmacy, Department of Pharmacognosy 35100 Bornova, Izmir Turkey,
Onur, M. A.
Ege University Faculty of Pharmacy, Department of Pharmacognosy 35100 Bornova, Izmir Turkey,
Kaya, G. I.
Ege University Faculty of Pharmacy, Department of Pharmacognosy 35100 Bornova, Izmir Turkey,
Ahmed, A. B. A.
Bharathidasan University Department of Plant Science Tiruchirappalli 620 024 Tamil Nadu India, dr.bakrudeenaliahmed@yahoo.co.in,
Rao, A. S.
Bharathidasan University Department of Biotechnology Tiruchirappalli 620 024 Tamil Nadu India,
Rao, M. V.
Bharathidasan University Department of Plant Science Tiruchirappalli 620 024 Tamil Nadu India,
Taha, R. M.
University of Malaya Institute of Biological Sciences, Faculty of Science 50603 Kuala Lumpur Malaysia,
Choi, Y.
Sungkyunkwan University School of Pharmacy Suwon, Gyeonggi-do Korea,
Shin, B. S.
Catholic University of Daegu College of Pharmacy Gyeongsan-si, Gyeongbuk Korea,
Kim, M. G.
Sungkyunkwan University School of Pharmacy Suwon, Gyeonggi-do Korea,
Shin, S. W.
Sungkyunkwan University School of Pharmacy Suwon, Gyeonggi-do Korea,
Kim, T. H.
Catholic University of Daegu College of Pharmacy Gyeongsan-si, Gyeongbuk Korea,
Jang, H. C.
Sungkyunkwan University School of Pharmacy Suwon, Gyeonggi-do Korea,
Yoo, S. D.
Sungkyunkwan University School of Pharmacy Suwon, Gyeonggi-do Korea, sdyoo@skku.ac.kr,
Dong, S.
The Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 China, sqdong@licp.cas.cn,
Shi, X.
Gansu Academy of Medical Sciences Lanzhou 730000 China,
Liu, Q.
Graduate University of the Chinese Academy of Sciences Beijing 100039 China,
Zhang, Z.
The Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 China,
Zhao, L.
The Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 China,
Kumar, N.
IPDO Dr. Reddy’s Laboratories Ltd. Bachupally, Hyderabad 500072 A.P India, navneetrajpoot21@yahoo.com,
Sangeetha, D.
.I.T. University Department of Chemistry, S.A.S. Vellore 632014 Tamilnadu India,
Goyal, R.
IPDO Dr. Reddy’s Laboratories Ltd. Bachupally, Hyderabad 500072 A.P India,
Reddy, P. S.
IPDO Dr. Reddy’s Laboratories Ltd. Bachupally, Hyderabad 500072 A.P India,
Abstrakty : A simple, selective, and stability-indicating reverse phase liquid chromatographic method has been developed and validated for the simultaneous determination of impurities and forced degradation products of quetiapine fumarate. The chromatographic separation was achieved on Inertsil-3 C8, 150 mm × 4.6 mm, 5 μm column at 35°C with UV detection at 217 nm using gradient mobile phase at a flow rate of 1.0 mL/min. Mobile phase A contains a mixture of 0.01 M di-potassium hydrogen orthophosphate (pH 6.8) and acetonitrile in the ratio of 80:20 (v/v), respectively, and mobile phase B contains a mixture of 0.01 M di-potassium hydrogen orthophosphate (pH 6.8) and acetonitrile in the ratio of 20:80 (v/v), respectively. The drug product was subjected to the stress conditions of oxidative, hydrolysis (acid and base), hydrolytic, thermal, and photolytic degradation. Quetiapine fumarate was found to degrade significantly in acid, base, and oxidative stress conditions. The degradation products were well resolved from main peak and its impurities. The mass balance was found to be in the range of 96.6–102.2% in all the stressed conditions, thus proved the stability-indicating power of the method. The developed method was validated as per ICH guidelines with respect to specificity, linearity, limit of detection and quantification, accuracy, precision, and robustness.

Słowa kluczowe : development, validation, forced degradation, stability-indicating, quetiapine fumarate, impurities,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2013
Numer : Vol. 25, no. 2
Strony : 393 – 409
Bibliografia : 1 J.G. Small, S.R. Hirsch, L.A. Arvanitis, B.G. Miller, and C.G. Link, Arch. Gen. Psychiatry, 54, 549 (1997)
2 http://www.rxlist.com/seroquel-drug.htm
3 N. Rajendraprasad, K. Basavaiah, and K. B. Vinay, J. Pre-Clin. Clin. Res., 4, 24 (2010)
4 K. Basavaiah, N. Rajendraprasad, P.J. Ramesh, and K.B. Vinay, Thai J. Pharm. Sci., 34, 146 (2010)
5 S.R. Dhaneshwar, N.G. Patre, and M.V. Mahadik, Acta Chromatogr., 21, 83 (2009)
6 B. Dhandapani, A. Somasundaram, S.H. Raseed, M. Raja, and K. Dhanabal, Int. J. PharmTech Res., 1, 139 (2009)
7 S.A. Jadhav, S.B. Landge, S.S. Shelke, N.C. Niphade, R.K. Panchangam, V.T. Mathad, S. R. Bembalkar, Chromatographia, 71, 1055 (2010)
8 D. Suneetha and A.L. Rao, E-J. Chem., 7, 261 (2010)
9 S. Hillaert, L. Snoeck and W. Van den Bossche, J. Chromatogr. A, 1033, 357 (2004)
10 N. El-Enany, A. El-Brashy, F. Belal, and N. El-Bahay, Port. Electrochim. Acta, 27, 113, (2009)
11 L. Mercolini, M. Grillo, C. Bartoletti, G. Boncompagni, and M.A. Raggi, Anal. Bioanal. Chem., 388, 235 (2007)
12 F. Belal, A. Elbrashy, M. Eid, and J.J. Nasr, J. Liq. Chromatogr. Relat. Technol., 31, 1283 (2008)
13 P.C. Davis, O. Bravo, M. Gehrke, and C.T. Azumaya, J. Pharm. Biomed. Anal., 51, 1113 (2010)
14 R. Mandrioli, S. Fanali, A. Ferranti, and M.A. Raggi, J. Pharm. Biomed. Anal., 30, 969 (2002)
15 Z. Zhou, X. Li, K. Li, Z. Xie, Z. Cheng, W. Peng, F. Wang, R. Zhu, and H. Li, J. Chromatogr. B, 802, 257 (2004)
16 E.U. Stolarczyk, L. Kaczmarek, K. Eksanow, M. Kubiszewski, M. Glice, and A. Kutner, Pharm. Dev. Technol., 14, 27 (2009)
17 H. Xu, D. Wang, C. Sun, Y. Pan, and M. Zhou, J. Pharm. Biomed. Anal., 44, 414 (2007)
18 Ch. Bharathi, K.J. Prabahar, Ch.S. Prasad, M.S. Rao, G.N. Trinadhachary, V.K. Handa, R. Dandala, and A. Naidu, Pharmazie, 63, 14 (2008)
19 V. Mittapelli, L. Vadali, D.A. Sivalakshmi, and M.V. Suryanarayana, Rasayan J. Chem., 3, 677 (2010)
20 R.K. Trivedi and M.C. Patel, Sci. Pharm., 79, 97 (2011)
21 S.R. Krishna, B.M. Rao and N.S. Rao, Rasayan J. Chem., 1, 466 (2008)
22 M. Bakshi and S. Singh, J. Pharm. Biomed. Anal., 28, 1011 (2002)
23 ICH Q1A (R2), Stability Testing of New Drug Substances and Products, 2000
24 ICH Q1B, Photostability testing on new drug substances and products, 1996
25 ICH Q2 (R1), Validation of Analytical Procedures: Text and Methodology, 2005
DOI :
Cytuj : Huo, H. L. ,Yu, S. H. ,Liu, X. Z. ,Meng, Y. ,Ren, Y. P. ,Zhang, L. T. ,Liu, L. ,Zhao, T. ,Cheng, X. M. ,Wang, C. H. ,Wang, Z. T. ,Gao, X. ,Jiang, X. ,Wang, L. ,Urban, J. ,Vaněk, J. ,Štys, D. ,Li, C. D. ,Liu, Y. ,Shi, Y. P. ,Zhang, Y. ,Peng, Q. ,Zeng, H. ,Yao, S. ,Zhang, Y. ,Song, H. ,Zhou, W. ,Zhang, X. Y. ,Lv, Y. P. ,Liu, X. D. ,Xu, C. ,Duan, G. L. ,Smoliński, A. ,Howaniec, N. ,Unver-Somer, N. ,Cicek-Polat, D. ,Emir, A. ,Onur, M. A. ,Kaya, G. I. ,Ahmed, A. B. A. ,Rao, A. S. ,Rao, M. V. ,Taha, R. M. ,Choi, Y. ,Shin, B. S. ,Kim, M. G. ,Shin, S. W. ,Kim, T. H. ,Jang, H. C. ,Yoo, S. D. ,Dong, S. ,Shi, X. ,Liu, Q. ,Zhang, Z. ,Zhao, L. ,Kumar, N. ,Sangeetha, D. ,Goyal, R. ,Reddy, P. S. , A validated stability-indicating RP-LC method for the estimation of process-related impurities and degradation products of quetiapine fumarate in solid oral dosage form. Acta Chromatographica Vol. 25, no. 2/2013
[Top]

Simultaneous and sensitive determination of eight coumarins in rat bile and urine after oral administration of Radix Angelicae Dahuricae extract by liquid chromatography-electrospray ionization-mass spectrometry

Czasopismo : Acta Chromatographica
Tytuł artykułu : Simultaneous and sensitive determination of eight coumarins in rat bile and urine after oral administration of Radix Angelicae Dahuricae extract by liquid chromatography-electrospray ionization-mass spectrometry

Autorzy :
Huo, H. L.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Yu, S. H.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Liu, X. Z.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Meng, Y.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Ren, Y. P.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Zhang, L. T.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China, zhanglantong@263.net,
Abstrakty : A selective and sensitive liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS) method was developed and validated for analysis of xanthotoxol (1), xanthotoxin (2), isoimpinellin (3), bergapten (4), oxypeucedanin (5), imperatorin (6), cnidilin (7), and isoimperatorin (8) in rat bile and urine using pimpinellin as an internal standard (IS). An Agilent 1200 liquid chromatography system (Agilent Technologies, USA) equipped with a quaternary pump, an autosampler, and a column compartment was used for all analyses. Chromatographic separations were performed on a Sapphire C18 column (150 mm × 4.6 mm, 5 μm), and the column temperature was maintained at 30°C; the sample injection volume was 10 μL. The specificity, linearity, accuracy, precision, recovery, matrix effect, and several stabilities were validated for all analytes in the rat bile and urine samples. The method was successfully applied in monitoring the concentrations of eight coumarins in rat bile and urine after a single oral administration of Radix Angelicae Dahuricae extract with a dosage of 8.0 mL/kg. In the bile samples, the eight coumarins excreted completely in twenty-four hours. The average percentages of coumarins (1–8) excreted were 0.045%, 0.019%, 0.177%, 0.105%, 0.337%, 0.023%, 0.024%, 0.021%. In the urine samples, the eight coumarins excreted completely in seventy-two hours. The average percentages of coumarins (1–8) excreted were 1.78%, 0.095%, 0.130%, 0.292%, 0.082%, 0.008%, 0.005%, 0.004%. The method is robust and specific and it can successfully complete the requirements of the excretion study of the eight coumarins in Radix Angelicae Dahuricae.

Słowa kluczowe : HPLC-MS/MS, Radix Angelicae Dahuricae, excretion, coumarins,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2013
Numer : Vol. 25, no. 2
Strony : 201 – 219
Bibliografia : 1 H. Zhu, D.Z. Kong, H.J. Xu, N. Wang, C.C. Zhao, P.W. Liu, and L.T. Zhang, Asian, J. Chem., 23, 3893–3897 (2011)
2 T.T. Wang, H. Jin, Q. Li, W.M. Cheng, Q.Q. Hu, X.H. Chen, and K.S. Bi, Chromatographia, 65, 477–481 (2007)
3 Y. Xie, W.Q. Zhao, T.T. Zhou, G.R. Fan, and Y.T. Wu, Phytochem. Anal., 21, 473–482 (2010)
4 L.P. Xiang, I.H. Park, S.H. Back, H.Y. Kim, M.K. Park, and J.H. Park, J. Ethnopharmacol., 93, 243–246 (2004)
5 Y. Kimura, H. Ohminami, H. Arichi, H. Okuda, K. Baba, M. Kozawa, and S. Arichi, J. Nat. Prod., 60, 249–251 (1997)
6 F. Cottiglia, G. Loy, D. Garau, C. Floris, M. Casu, R. Pompei, and L. Bonsignore, Phytomedicine, 8, 302–305 (2001)
7 Y.S. Kwon, A. Kobayashi, S. Kajiyama, K. Kawazu, H. Kanzaki, and C.M. Kim, Phytochemistry, 44, 887–889 (1997)
8 S.X. Deng and Y.Q. Mo, Chin. Trad. Herbal Drugs 19, 22–25 (1988)
9 B.S. Adama, R. Pentza, C.P. Siegersa, O. Strubelt, and M. Tegtmeier, Phytomedicine, 12, 52–61 (2005)
10 J.Y. Deng, G.H. Gao, C.J. Zhao, and P.J. Xu, J. Shenyang Pharmaceut. Univ., 21, 354–357 (2004)
11 A.Y. Park, S.Y. Park, J. Lee, M. Jung, J. Kim, S.S. Kang, J.R. Youm, and S.B. Han, Biomed. Chromatogr., 23, 1034–1043 (2009)
12 X.G. Zheng, X.W. Zhang, X.N. Sheng, Z.F. Yuan, W. Yang, Q. Wang, and L.T. Zhang, J. Pharmaceut. Biomed. Anal., 51, 599–605 (2010)
13 W. Yang, C. Feng, D.Z. Kong, X.W. Shi, X.G. Zheng, Y. Cui, M. Liu, L.T. Zhang, and Q. Wang, Food Chem., 120, 886–894 (2010)
14 Y.B. Li, J. Pharmaceut. Biomed. Anal., 40, 1253–1256 (2006)
15 Y. Xie, Y. Chen, M. Lin, J. Wen, G.R. Fan, and Y.T. Wu, J. Pharmaceut. Biomed. Anal., 44, 166–172 (2007)
16 S.C. Wang, Q.H. Chen, and L.C. He, J. Chromatogr. B, 852, 473–478 (2007)
17 T.F. Jing, Z.H. Lv, Y.H. Wang, and M.E. Yue, Biomed. Chromatogr., 24, 581–587 (2010)
18 W. Yang, C. Feng, D.Z. Kong, X.W. Shi, Y. Cui, M. Liu, Q. Wang, Y.L. Wang, and L.T. Zhang, J. Chromatogr. B, 878, 575–582 (2010)
19 Y.F. Du, P.W. Liu, X.W. Shi, Y.R. Jin, Q. Wang, X.W. Zhang, X.N. Sheng, and L.T. Zhang, Anal. Biochem., 407, 111–119 (2010)
20 H. Zhu, L.T. Zhang, Y.P. Ren, H.J. Xu, and Y. Cui, Chin. J. Pharmaceut., 5, 112–115 (2011)
DOI :
Cytuj : Huo, H. L. ,Yu, S. H. ,Liu, X. Z. ,Meng, Y. ,Ren, Y. P. ,Zhang, L. T. , Simultaneous and sensitive determination of eight coumarins in rat bile and urine after oral administration of Radix Angelicae Dahuricae extract by liquid chromatography-electrospray ionization-mass spectrometry. Acta Chromatographica Vol. 25, no. 2/2013
[Top]

Characterization and determination of trace alkaloids in seeds extracts from Peganum harmala linn. Using LC-ESI-MS and HPLC

Czasopismo : Acta Chromatographica
Tytuł artykułu : Characterization and determination of trace alkaloids in seeds extracts from Peganum harmala linn. Using LC-ESI-MS and HPLC

Autorzy :
Huo, H. L.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Yu, S. H.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Liu, X. Z.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Meng, Y.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Ren, Y. P.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Zhang, L. T.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China, zhanglantong@263.net,
Liu, L.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Zhao, T.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Cheng, X. M.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Wang, C. H.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China, wchcxm@hotmail.com,
Wang, Z. T.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China, wangzt@hotmail.com,
Abstrakty : Harmaline and harmine accounted for more than 70% in composition in extracts of P. harmala. More attention, however, should be paid to the other alkaloids which would be favorable or unfavorable to the efficacy and safety of the products. It was necessary to determine these trace alkaloids in the extracts; thereafter, most of them have been characterized. Diglycoside vasicine, vasicine, vasicinone, harmalol, harmol, tetrahydroharmine, 8-hydroxy-harmine, ruine, harmaline, and harmine were separated and identified with reference substances and characteristic MS spectra in extracts by liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) and high-performance liquid chromatography (HPLC). Three trace alkaloids, vasicine, harmalol, and harmol were determined using the developed chromatographic separation method subsequently. The average contents of vasicine, harmalol, and harmol in extracts of ten batches were 2.53 ± 0.73, 0.54 ± 0.19, and 0.077 ± 0.03%, respectively. The total content of the three alkaloids was 3.23 ± 0.90% (from 1.81 to 4.48%). For rough estimation of all the relative alkaloids except of harmaline and harmine, the average total areas of all peaks in extracts varied from 4.35 to 26.64% detected at 220, 254, 265, 280, and 380 nm, respectively. The results indicated that area normalization method was powerless for the quality evaluation for traditional herb medicine consisting of numerous compounds with highly differential features. It might be concluded that LC-MS or HPLC could be utilized as a qualitative and quantitative analytical method for quality control of the extracts from seeds of P. harmala L.

Słowa kluczowe : Peganum harmala L, extracts, alkaloids, characterization, quality control, LC-ESI-MS,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2013
Numer : Vol. 25, no. 2
Strony : 221 – 240
Bibliografia : 1 L. EL-Bahri and R. Chemli, Vet. Hum. Toxicol., 33, 276–277 (1991)
2 M. Massoud, J. Hossein, and S. Porooz, Iran. J. Pharmacol. Ther., 1, 1–4 (2002)
3 C.H. Wang, J. Liu, L.M. Zheng, Y.M. Lin, X.G. Zou, M. Chen, and D.J. Sun, Chin. Pharm. J., 37, 211–215 (2002)
4 J.S. Glasby, Encyclopedia of the alkaloids, Plenum Press, New York, 1975, p. 658
5 O.Y. Khan, Studies in the chemical constituents of Peganum harmala, University of Karachi Research Institute of Chemistry, Doctor thesis, Pakistan, 1990, pp. 26–41
6 B. Blum, H. Weizmann, G.M. Simpson, D. Krasilowsky, I.S. Kulcsar, and H. Merskey, Psychopharmacologia, 6, 307–310 (1964)
7 N. Ginovart, J.H. Meyer, A. Boovariwala, D. Hussey, E.A. Rabiner, S. Houle, and A.A. Wilson, J. Cereb. Blood Flow Metab., 3, 330–344 (2006)
8 M. Iurlo, G. Leone, B. Schilström, L. Linnér, G. Nomikos, P. Hertel, B. Silvestrini, and H. Svensson, Psychopharmacology, 159, 98–104 (2001)
9 Y. Song, D. Kesuma, J. Wang, Y. Deng, J. Duan, J.H. Wang, and R.Z. Qi, Biochem. Biophys. Res. Commun., 317, 128–132 (2004)
10 E.E. Wright, J.L. Bird, J.M. Feldman, Res. Commun. Chem. Pathol. Pharmacol., 24, 259–272 (1979)
11 M. Canessa, E. Jaimovich, and M. de la Fuente, J. Membr. Biol., 13, 263–282 (1973)
12 Z.Y. Song, J.R. Liu, X.L. Lu, and L.J. Wang, Chin. Med. Mat., 29, 571–573 (2006)
13 J. Jiménez, L. Riverón-Negrete, F. Abdullaev, J. Espinosa-Aguirre, and R. Rodríguez-Arnaiz, Exp. Toxicol. Pathol., 60, 381–389 (2008)
14 E.N. Gaviraj, G.R. Babu, and U.D. Murthy, Indian Drugs, 35, 471–474 (1998)
15 A. Astulla, K. Zaima, Y. Matsuno, Y. Hirasawa, W. Ekasari, A. Widyawaruyanti, N.C. Zaini, and H. Morita, Nat. Med., 62, 470–472 (2008)
16 G.C. Di, F. Delmas, E. Ollivier, R. Elias, G. Balansard, and P. Timon-David, Exp. Parasitol., 106, 67–74 (2004)
17 D. Bian, G.H. Li, and H.Q. Zhang, Acta Pharmacol. Sin., 8, 477–480 (1987)
18 J. Bruinvels and T.L. Sourkes, Eur. J. Pharmacol., 4, 31–39 (1968)
19 D.H. Aarons, G.V. Rossi, and R.F. Orzechowski, J. Pharm. Sci., 66, 1244–1248 (1977)
20 X.Y. Zheng, Z.J. Zhang, G.X. Chou, T. Wu, X.M. Cheng, C.H. Wang, and Z.T. Wang, Arch. Pharm. Res., 32, 1245–1251 (2009)
21 X.M. Chen, Y.Q. Liu, H.D. Xie, C.H. Wang, and Z.T. Wang, Chin. J. Pharm., 39, 443–446 (2008)
22 O.P. Gupta, K.K. Anand, B.J. Ghatak, and C.K. Atal, Indian J. Exp. Biol., 16, 1075–1077 (1978)
23 C.H. Wang, J. Liu, and Y.M. Lin, Chin. Hosp. Pharm. J., 22, 139–141 (2002)
24 R. Zayed and M. Wink, Z. Naturforsch. C, 60 451–458 (2005)
25 R. Kikura-Hanajiri, M. Hayashi, K. Saisho, and Y. Goda, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 825, 29–37 (2005)
26 F. Bianchi, M. Careri, C. Corradini, I. Elviri, A. Mangia, and I. Zagnoni, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 825, 193–200 (2005)
27 M.T. Galceran, E. Moyano, L. Puignou, and P. Pais, J. Chromatogr. A, 730, 185–194 (1996)
28 J.A. Duan, R.H. Zhou, S.X. Zhao, M.S. Wang, and Z.T. Che, J. Chin. Pharm. Univ., 29, 21–23 (1998)
29 X.L. Tian, D.J. Sun, and N.S. Du, J. Xinjiang Med. Univ., 26, 113–116 (2003)
30 W. Schliemann, B. Schneider, V. Wray, J. Schmidt, M. Nimtz, A. Porzel, and H. Böhm, Phytochemistry, 67, 191–201 (2006)
DOI :
Cytuj : Huo, H. L. ,Yu, S. H. ,Liu, X. Z. ,Meng, Y. ,Ren, Y. P. ,Zhang, L. T. ,Liu, L. ,Zhao, T. ,Cheng, X. M. ,Wang, C. H. ,Wang, Z. T. , Characterization and determination of trace alkaloids in seeds extracts from Peganum harmala linn. Using LC-ESI-MS and HPLC. Acta Chromatographica Vol. 25, no. 2/2013
[Top]

Using LC-MS/MS to study dauricine pharmacokinetics and determine its bioavailability following administration in different routes

Czasopismo : Acta Chromatographica
Tytuł artykułu : Using LC-MS/MS to study dauricine pharmacokinetics and determine its bioavailability following administration in different routes

Autorzy :
Huo, H. L.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Yu, S. H.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Liu, X. Z.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Meng, Y.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Ren, Y. P.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Zhang, L. T.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China, zhanglantong@263.net,
Liu, L.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Zhao, T.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Cheng, X. M.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Wang, C. H.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China, wchcxm@hotmail.com,
Wang, Z. T.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China, wangzt@hotmail.com,
Gao, X.
Sichuan University Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy Chengdu, Sichuan China, gaomuxouzi@126.com,
Jiang, X.
Sichuan University Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy Chengdu, Sichuan China,
Wang, L.
Sichuan University Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy Chengdu, Sichuan China,
Abstrakty : Dauricine has a variety of pharmacological properties including anti-inflammatory, anti-arrhythmic, and antihypertensive effects as well as reversing multidrug resistance (MDR) of cancer cells. While its therapeutic application is increasing, its bioavailability of different administration routes has not been studied. In the present study, we developed and validated a liquid chromatography/electrospray ionization mass spectrometry method (LC-MS/MS). Using this method, we quantified dauricine in rat plasma after administration via intravenous (i.v.) injection, per oral (p.o.), and intraperitoneal injection (i.p.). Our results indicated that this method detected plasma dauricine with a good linearity in the range of 1.95–1000.00 ng/mL (r = 0.9997). The extraction method showed an average intra- and inter-day recovery of 98.21–104.35% and 98.0–103.58%, respectively. Dauricine showed a fast absorption and widespread distribution after administration in all three tested routes. After intravenous administration (2.5, 5.0, 10.0 mg/kg), the pharmacokinetics of dauricine exhibited a first-order kinetics. In addition, dauricine showed a slow elimination with a long half-life (t1/2z) and double peaks phenomenon following p.o. and i.p. administration. Furthermore, using area under the plasma concentration-time curve (AUC), we calculated absolute bioavailability, which was over twofold higher when administered via i.p. than via p.o. administration. The newly obtained information from our study will provide important reference for dauricine dose and administration route in designing dauricine therapy for applicable diseases.

Słowa kluczowe : dauricine, LC-MS/MS, rat plasma, pharmacokinetic study, absolute bioavailability,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2013
Numer : Vol. 25, no. 2
Strony : 241 – 256
Bibliografia : 1 M.C. Li, D.Y. Huang, Chin. Pharm. J., 43, 1875–1877 (2008)
2 Z.K. Huang, Modern Journal of Integrated Traditional Chinese and Western Medicine, 13, 718–719 (2004)
3 Q.Y. Li, J.Y. Wang, J.J. Cheng, Progress in Veterinary Medicine, 31, 115–117 (2010)
4 Pharmacopoeia Commission of People’s Republic of China. Pharmacopoeia of People’s Republic of China, 1st edn. Chemical Industry Press, Beijing, 2005
5 Y. Zhang, C. Sun, Q.C. Guan, et al. Modern Pharmacy and Clinic, 24, 367–368 (2009)
6 M. Li, B.E. Shan, International Journal of Traditional Chinese Medicine, 27, 267–271 (2005)
7 X.Q. Zhu, T.G. Han, Journal of Chengde Medical College, 24, 418–420 (2007)
8 X.D. Huang, D. Han, X.Y. Cui, Medical Journal of Chinese People’s Health, 19, 575–576 (2007)
9 J.Q. Qian, Acta Pharmacol Sin, 23, 1086–1092 (2002)
10 H. Tian, Q.C. Pan, Cancer, 15, 410–414 (1996)
11 J.H. Li, F.Q. Qin, P.M. Yang, Journal of Dalian Medical University, 24, 94–96 (2002)
12 L. He, G.Q. Liu, Acta Pharmacol Sin, 23, 591–596 (2002)
13 F.M. Han, Z.H. Peng, W. Song, et al. Journal of Chromatography B, 854, 1–7 (2007)
14 X.Y. Liu, Q. Liu, D.M. Wang, et al. Journal of Chromatography B, 878, 1199–1203 (2010)
15 S.J. Chen, B. Zhang, Y.M. Yang, Z.S. Dai, et al. Chinese Journal Of Clinical Pharmacology and Therapeutics, 5, 213–217 (2000)
16 S.J. Chen, Y.M. Yang, Chinese Pharmacological Bulletin, 17, 225–229 (2001)
17 R. Sun, C. Wang, Chinese Journal of Pharmacovigilance, 6, 546–549 (2009)
18 V.J. Wacher, L. Salphati, L.Z. Benet, Advanced Drug Delivery Reviews, 46, 89–102 (2001)
DOI :
Cytuj : Huo, H. L. ,Yu, S. H. ,Liu, X. Z. ,Meng, Y. ,Ren, Y. P. ,Zhang, L. T. ,Liu, L. ,Zhao, T. ,Cheng, X. M. ,Wang, C. H. ,Wang, Z. T. ,Gao, X. ,Jiang, X. ,Wang, L. , Using LC-MS/MS to study dauricine pharmacokinetics and determine its bioavailability following administration in different routes. Acta Chromatographica Vol. 25, no. 2/2013
[Top]

Unsupervised adaptive filter for baseline thresholding and elimination in liquid chromatography-mass spectrometry via approximation of the standard deviation of baseline distribution in retention time domain

Czasopismo : Acta Chromatographica
Tytuł artykułu : Unsupervised adaptive filter for baseline thresholding and elimination in liquid chromatography-mass spectrometry via approximation of the standard deviation of baseline distribution in retention time domain

Autorzy :
Huo, H. L.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Yu, S. H.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Liu, X. Z.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Meng, Y.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Ren, Y. P.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Zhang, L. T.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China, zhanglantong@263.net,
Liu, L.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Zhao, T.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Cheng, X. M.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Wang, C. H.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China, wchcxm@hotmail.com,
Wang, Z. T.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China, wangzt@hotmail.com,
Gao, X.
Sichuan University Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy Chengdu, Sichuan China, gaomuxouzi@126.com,
Jiang, X.
Sichuan University Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy Chengdu, Sichuan China,
Wang, L.
Sichuan University Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy Chengdu, Sichuan China,
Urban, J.
University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, School of Complex Systems (former Institute of Physical Biology), Laboratory of Applied System Biology Zamek 136 Nove Hrady 373 33 Czech Republic, urbanj@frov.jcu.cz,
Vaněk, J.
University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, School of Complex Systems (former Institute of Physical Biology), Laboratory of Applied System Biology Zamek 136 Nove Hrady 373 33 Czech Republic,
Štys, D.
University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, School of Complex Systems (former Institute of Physical Biology), Laboratory of Applied System Biology Zamek 136 Nove Hrady 373 33 Czech Republic,
Abstrakty : The omitting presence of a baseline (background, systemic noise, mobile phase) in liquid chromatography-mass spectrometry (LC-MS) measurements impedes objective analysis. Therefore, there is a demand to remove its contribution from the signal response. Elimination of baseline contribution is justified by increasing the data mining output, both qualitatively and quantitatively. Behavior of the baseline content is not perfectly constant on the time axis, and it is often necessary to experiment with gradient changes. However, the behavior could be parametrized using a technique derived from statistical moments. In this article, we propose adaptive thresholding as an unsupervised method for baseline removal from the measurement data. Results of a real analyte measurement are discussed to illustrate its efficiency.

Słowa kluczowe : LC-MS, mobile phase, systemic noise, baseline removal, adaptive thresholding,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2013
Numer : Vol. 25, no. 2
Strony : 257 – 273
Bibliografia : 1 V.P. Andreev, T. Rejtar, H.-S. Chen, E.V. Moskovets, A.R. Ivanov, and B.L. Karger, Anal. Chem., 75, 6314–6326 (2003)
2 J.R. Kettman, C. Coleclough, J.R. Frey, and I. Lefkovits, Proteomics, 2(6), 624–631 (2002)
3 G.L. Corthals, V.C. Wasinger, D.F. Hochstrasser, and J.C. Sanchez, Electrophoresis, 21(6), 1104–1115 (2000)
4 P. Du, W.A. Kibbe, and S.M. Lin, Bioinformatics, 22(17), 2059–2065 (2006)
5 A. Mihailova, E. Lundanes, and T. Greibrokk, J. Sep. Sci., 29, 576–581 (2006)
6 T. Pluskal, S. Castillo, A. Villar-Briones, and M. Orešič, BMC Bioinformatics, 11, 395 (2010)
7 R.A. Viscarra Rossel, Chemom. Intell. Lab. Syst., 90, 72–83 (2008)
8 N. Hoffman and J. Stoye, Bioinformatics, 25(16), 2080–2081 (2009)
9 J. Urban, J. Vanek, J. Soukup, and D. Stys, Bioinformatics, 25, 2764–2767 (2009)
10 A. Sauve and T. Speed, Normalization, baseline correction and alignment of high-throughput mass spectrometry data, Proceedings of the Genomic Signal Processing and Statistics, 2004
11 M. Sonka, V. Hlavac, and R. Boyle, Image processing, analysis and machine vision, Brooks/Cole Publishing Company, USA, 1999
12 R.C. Gonzales and R.E. Woods, Digital image processing, Addison-Wesley Publishing Company, MA, 1992
13 S. Beucher, Applications of mathematical morphology in material sciences: a review of recent developments, International Metallography Conference, 1995, pp. 41–46
14 L. Vincent, Morphological grayscale reconstruction in image analysis: applications and efficient algorithms, IEEE Transactions on Image Processing, 1993, pp. 176–201
15 Zatkova, M. Sergejevova, J. Urban, R. Vachta, D. Stys, and J. Masojidek, Aquacult. Nutr., 17(3), 278–286 (2011)
16 D. Stys, J. Urban, J. Vanek, and P. Cisar, Analysis of biological time-lapse microscopic experiment from the point of view of the information theory, Micron, S0968-4328(10)00026-0 (2010)
17 D. Stys, J. Vanek, T. Nahlik, J. Urban, and P. Cisar, Mol. Biosyst., 7(10), 2824–2833 (2011)
18 DI, Malyarenko, W.E. Cooke, B.L. Adam, G. Malik, H.J. Chen, E.R. Tracy, M.W. Trosset, M. Sasinowski, O.J. Semmes, and D.M. Manos, Clin. Chem., 51(1), 65–74 (2005)
19 J.S. Morris, K.R. Coombes, J. Koomen, K.A. Baggerly, and R. Kobayashi, Bioinformatics 21(9), 1764–1775 (2005)
20 S.M. Norton, Methods for time-alignment of liquid chromatography-mass spectrometry data, Patent 6989100, 2006
21 K.J. Johnson, B.W. Wright, K.H. Jarman, and R.E. Synovec, High-speed peak matching algorithm for retention time alignment of gas chromatographic data for chemometric analysis, Elsevier Science B.V., Amsterdam, 2003
22 M. Chae, R.J. Shmookler Reis, and J.J. Thaden, BMC Bioinformatics, 9(Suppl 9), S15 (2008)
23 P. Hrouzek, P. Tomek, A. Lukesova, J. Urban, L. Voloshko, B. Pushparaj, S. Ventura, J. Lukavsky, D. Stys, and J. Kopecky, Environ. Toxicol., 26(4), 345–358 (2011)
24 V. Makarenkov, P. Zentilli, D. Kevorkov, A. Gagarin, N. Malo, and R. Nadon, Bioinformatics, 23(13), 1648–1657 (2007)
25 T. Fouquet and L. Charles, J. Am. Soc. Mass Spectrom., 21, 60–67 (2010)
26 M. Holčapek, K. Volná, P. Jandera, L. Kolářová, K. Lemr, M. Exner, and A. Církva, J. Mass Spectrom., 39, 43–50 (2004)
27 L.C. Herrera, J.S. Grossert, and L. Ramaley, J. Am. Soc. Mass Spectrom., 12, 1926–1941 (2008)
28 V. Shulaev, Brief. Bioinform., 7, 128–139 (2006)
29 S.A. Gustavsson, J. Samskog, K.E. Markides, and B. Langstrom, J. Chromatogr. A, 937(41) (2001)
30 M. Bantscheff, M. Schirle, G. Sweetman, J. Rick, and B. Kuster, Anal. Bioanal. Chem., 389, 1017–1031 (2007) DOI 10.1007/s00216-007-1486-6
31 W. Li, J. Zhang, and F.L. Tse, Biomed. Chromatogr., DOI 10.1002/bmc.1572
32 K. Sadilkova, S.M. Jr. Gospe, and S.H. Hahn, J. Neurosci. Methods, 184, 136–141 (2009)
33 A. Andersson and H. Ehrsson, J. Pharm. Biomed. Anal., 13(4–5), 639–644 (1995)
34 S. Pihl, A. Karina, T. Huusom, M. Rohde, M. Poulsen, M. Jørgensen, and M.A. Kal, Bioanalysis, 2(6), 1041–1049 (2010)
35 P.A. Aronov, L.M. Hall, K. Dettmer, C.B. Stephensen, and B.D. Hammock, Anal. Bioanal. Chem., 391(5), 1917–1930 (2008)
36 W. Li, S. Luo, H.T. Smith, and F.L. Tse, J. Chromatogr. B: Analyt. Technol. Biomed. Life Sci., 878(5–6), 583–589 (2010)
37 A.S. Christophersen, J. Analyt. Toxicol., 10(4), 129–131 (1986)
38 P. Kohl and S. Medlar, Occurrence of Manganese in Drinking Water and Manganese Control, American Water Works Association, 2007
39 C.F. Bunting, IEEE T. Electromagn. C, 44(1), 214–221 (2002)
40 L. Chang, Z. Yonghong, and S. Attallah, Max-To-Mean Ratio Detection for Cognitive Radio, Vehicular Technology Conference, 1959–1963 (2008)
41 R.J. Hyndman, The problem with Sturges’ rule for constructing histograms, working papers, Monash University, Australia (1995)
42 G. Polya, How To Solve It: A New Aspect of Mathematical Method, Princeton University Press, Princeton, NJ, 1945
43 T. Golakoti, W.Y. Yoshida, S. Chaganty, and R. Moore, Tetrahedron, 56, 9093–9102 (2000)
44 G. Giatting, P. Gletting, S.N. Reske, K. Hohl, and C. Ring, Med. Phys., 34(11), 4285–4292 (2007)
45 W. Li and D.R. Nyholt, Genet. Epidemiol., 21(Supp 1), S272–S277 (2001)
46 S. Forni, M. Piles, A. Blasco, L. Varona, H.N. Oliveira, R.B. Lobo, and L.G. J. Anim. Sci., 0845 (2008)
47 K. Zcychaluk and D.H. Foster, Perception, 36, ECVP Supplement (2007)
48 A. Polettini (ed.), Applications of LC-MS in Toxicology, Pharmaceutical Press, 2006
49 J.T. Prince and E.M. Marcotte, Analyt. Chem., 78(17), 6140–6152 (2006)
50 M. Cannataro, G. Cuda, M. Gaspari, S. Greco, G. Tradigo, and P. Veltri, BMC Bioinformatics, 8, 255 (2007)
51 O.A. Sykes, An Introduction to Regression Analysis, The Inaugural Coase Lecture, 1999
52 M. Ledvij, Curve Fitting Made Easy, The Industrial Physicist, 2003, pp. 24–27
53 J. Reed, Curve Fitting, Lessons on Introduction to Statistics and Probability, http://argyll.epsb.ca/jreed/ , 2000
DOI :
Cytuj : Huo, H. L. ,Yu, S. H. ,Liu, X. Z. ,Meng, Y. ,Ren, Y. P. ,Zhang, L. T. ,Liu, L. ,Zhao, T. ,Cheng, X. M. ,Wang, C. H. ,Wang, Z. T. ,Gao, X. ,Jiang, X. ,Wang, L. ,Urban, J. ,Vaněk, J. ,Štys, D. , Unsupervised adaptive filter for baseline thresholding and elimination in liquid chromatography-mass spectrometry via approximation of the standard deviation of baseline distribution in retention time domain. Acta Chromatographica Vol. 25, no. 2/2013
[Top]

Simultaneous determination of six alkaloids in Phellodendron amurense by high-performance liquid chromatography

Czasopismo : Acta Chromatographica
Tytuł artykułu : Simultaneous determination of six alkaloids in Phellodendron amurense by high-performance liquid chromatography

Autorzy :
Huo, H. L.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Yu, S. H.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Liu, X. Z.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Meng, Y.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Ren, Y. P.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Zhang, L. T.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China, zhanglantong@263.net,
Liu, L.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Zhao, T.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Cheng, X. M.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Wang, C. H.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China, wchcxm@hotmail.com,
Wang, Z. T.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China, wangzt@hotmail.com,
Gao, X.
Sichuan University Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy Chengdu, Sichuan China, gaomuxouzi@126.com,
Jiang, X.
Sichuan University Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy Chengdu, Sichuan China,
Wang, L.
Sichuan University Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy Chengdu, Sichuan China,
Urban, J.
University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, School of Complex Systems (former Institute of Physical Biology), Laboratory of Applied System Biology Zamek 136 Nove Hrady 373 33 Czech Republic, urbanj@frov.jcu.cz,
Vaněk, J.
University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, School of Complex Systems (former Institute of Physical Biology), Laboratory of Applied System Biology Zamek 136 Nove Hrady 373 33 Czech Republic,
Štys, D.
University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, School of Complex Systems (former Institute of Physical Biology), Laboratory of Applied System Biology Zamek 136 Nove Hrady 373 33 Czech Republic,
Li, C. D.
Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P.R. China,
Liu, Y.
Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P.R. China,
Shi, Y. P.
Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P.R. China, shiyp@licp.cas.cn,
Abstrakty : A high-performance liquid chromatography (HPLC) method has been developed for simultaneous determination of six alkaloids, i.e., (−)-(R)-platydesmin, noroxyhydrastinine, berberine, skimmianine, canthin-6-one, and pteleine in the herbal medicine of Phellodendron amurense Rupr. The optimal condition for extraction and separation was achieved with a linear mobile phase gradient prepared from 0.1% phosphoric acid and acetonitrile. The LODs and LOQs for the analytes ranged from 0.06 to 0.22 μg mL-1and from 0.25 to 0.80 μg mL-1, respectively. The optimized method was applied to the determination of alkaloids in P. amurense Rupr. and was found to be efficient. This method can provide a scientific and technical platform to the manufacturers for setting up a quality control standard as well as to the public for quality and safety assurance of the proprietary traditional Chinese medicines.

Słowa kluczowe : high-performance liquid chromatography, phytochemical analysis, traditional Chinese medicine, Phellodendron amurense, alkaloids,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2013
Numer : Vol. 25, no. 2
Strony : 275 – 285
Bibliografia : 1 The State Pharmacopoeia Commission of the P.R. China (2005): Pharmacopoeia of the P.R. China. Chemical Industry Press, Beijing, p. 99
2 M. Murayama, T. Mori, H. Bando, T. Amiya, J. Ethnopharmacol., 35, 159 (1991)
3 X.K. Deng, W. Yin, W.D. Li, F.Z. Yin, X.Y. Lu, X.C. Zhang, Z.C. Hua, B.C. Cai, J. Ethnopharmacol., 106, 179 (2006)
4 S.A. Hill, S.J. Lonergan, J. Denekamp, D.J. Chaplin, Eur. J. Cancer, 29, 1320 (1993)
5 A. Nicholas, C. Jenny, C.N. Edouard, G.W. Keith, Bioorg. Med. Chem. Lett., 17, 3741 (2007)
6 F. Angelo, C. Paolo, W. Solimabi, G.N. Chandrakant, C. Guido, Tetrahedron, 56, 7305 (2000)
7 H. Yue, Z.F. Pi, F.R. Song, Z.Q. Liu, Z.W. Cai, S.Y. Liu, Talanta, 77, 1800 (2009)
8 M. Lombardo-Agüí, C. Cruces-Blanco, A.M. García, J. Chromatogr. B, 877, 833 (2009)
9 J.Y. Yin, Y.H. Xu, J. Li, E. Wang, Talanta, 75, 38 (2008)
10 L. Qin, L. Christine, G.C. Copper, Anal. Chim. Acta, 572, 205 (2006)
11 R. Gotti, J. Fiori, M. Bartolini, V. Cavrini, J. Pharm. Biomed. Anal., 42, 17 (2006)
12 G.M. Peter, Phytochemistry, 38, 1315 (1995)
13 T.R. Baggi, R.N.V. Rama, H.R.K. Murty, Forensic Sci. Int., 8, 265 (1976)
14 F. Pellati, S. Benvenuti, J. Pharm. Biomed. Anal., 48, 254 (2008)
15 Q.Q. Wu, Y.H. Gu, J. Pharm. Biomed. Anal., 40, 993 (2006)
16 C.O. Chan, C.C. Chu, K.W. Daniel, F.T. Chau, Anal. Chim. Acta., 592, 121 (2007)
17 X.H. Li, Ph.D. Dissertation of Graduate University of Chinese Academy of Sciences, 2009, p 60
18 J. Pusset, J.L. Lopez, M.A. Neirabeyeh, J.M. Veillon, Planta Med., 57, 153 (1991)
19 D.R. Baoyd, N.D. Sharan, S.A. Barr, J.G. Carroll, D. Mackerracher, J. Malone, J. Chem. Soc. Perkin Trans., I, 3397 (2000)
20 V. Fajardo, V. Elangno, B.K. Casels, M. Shamma, Tetrahedron Lett., 23, 39 (1982)
21 Y. Liu, J. Chen, X.H. Li, Y.P. Shi, J. AOAC Int., 93, 5 (2010)
DOI :
Cytuj : Huo, H. L. ,Yu, S. H. ,Liu, X. Z. ,Meng, Y. ,Ren, Y. P. ,Zhang, L. T. ,Liu, L. ,Zhao, T. ,Cheng, X. M. ,Wang, C. H. ,Wang, Z. T. ,Gao, X. ,Jiang, X. ,Wang, L. ,Urban, J. ,Vaněk, J. ,Štys, D. ,Li, C. D. ,Liu, Y. ,Shi, Y. P. , Simultaneous determination of six alkaloids in Phellodendron amurense by high-performance liquid chromatography. Acta Chromatographica Vol. 25, no. 2/2013
[Top]

HPLC determination of enantiomeric thiazolidine-2-carboxylic acid on chiral stationary phase with pre-column derivatization

Czasopismo : Acta Chromatographica
Tytuł artykułu : HPLC determination of enantiomeric thiazolidine-2-carboxylic acid on chiral stationary phase with pre-column derivatization

Autorzy :
Huo, H. L.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Yu, S. H.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Liu, X. Z.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Meng, Y.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Ren, Y. P.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Zhang, L. T.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China, zhanglantong@263.net,
Liu, L.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Zhao, T.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Cheng, X. M.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Wang, C. H.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China, wchcxm@hotmail.com,
Wang, Z. T.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China, wangzt@hotmail.com,
Gao, X.
Sichuan University Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy Chengdu, Sichuan China, gaomuxouzi@126.com,
Jiang, X.
Sichuan University Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy Chengdu, Sichuan China,
Wang, L.
Sichuan University Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy Chengdu, Sichuan China,
Urban, J.
University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, School of Complex Systems (former Institute of Physical Biology), Laboratory of Applied System Biology Zamek 136 Nove Hrady 373 33 Czech Republic, urbanj@frov.jcu.cz,
Vaněk, J.
University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, School of Complex Systems (former Institute of Physical Biology), Laboratory of Applied System Biology Zamek 136 Nove Hrady 373 33 Czech Republic,
Štys, D.
University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, School of Complex Systems (former Institute of Physical Biology), Laboratory of Applied System Biology Zamek 136 Nove Hrady 373 33 Czech Republic,
Li, C. D.
Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P.R. China,
Liu, Y.
Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P.R. China,
Shi, Y. P.
Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P.R. China, shiyp@licp.cas.cn,
Zhang, Y.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China, hangsong@vip.sina.com,
Peng, Q.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Zeng, H.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Yao, S.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Zhang, Y.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Song, H.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Abstrakty : A new high-performance liquid chromatography (HPLC) method has been developed and validated for determination of enantiomeric purity of thiazolidine-2-carboxylic acid within a short run time of less than 10 min. The method was based on pre-column derivatization of thiazolidine-2-carboxylic acid with aniline, and complete separation of enantiomers has been achieved on a Chiralcel OD-H analytical column (250 × 4.6 mm) using n-hexane-isopropanol (85:15 v/v) as mobile phase at a flow rate of 1.0 mL min-1under UV and optical rotation (OR) detection. Detection wavelength was set at 254 nm. Then the effects of mobile phase and temperature on enantioselectivity were further evaluated. The method was validated with respect to precision, accuracy, linearity, limit of detection (LOD), limit of quantification (LOQ), and robustness. The recoveries were between 98.5 and 101.3% with percentage relative standard deviation less than 1.16%. The LOD and LOQ for the aniline derivatives of (+)-thiazolidine-2-carboxylic acid were 4.9 and 16.4 μg mL-1and for the aniline derivatives (−)-thiazolidine-2-carboxylic acid were 5.1 and 17.2 μg mL-1, respectively.

Słowa kluczowe : liquid chromatography, enantiomeric purity, thiazolidine-2-carboxylic acid, pre-column derivatization, method validation,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2013
Numer : Vol. 25, no. 2
Strony : 287 – 296
Bibliografia : 1 S. Nain, M. Arya, V. Gupta, R. Sirohi, and D. Kishore, Int. J. Chem. Sci., 7, 1255(2009)
2 S. Nain, V. Gupta, M. Arya, R. Sirohi, and K.D. Reenu, J. Indian Chem. Soc., 86, 986 (2009)
3 S. Pushpan, U. Ramachandran, M. Kundu, V. Anantharaman et al., PCT Int. Appl., WO2009001192 (2008)
4 G. Cremonesi, C.P. Dalla, and R.C. La, Helv. Chim. Acta, 88, 1580 (2005)
5 A. Garofalo, F. Grande, A. Brizzi, F. Aiello, R. Dayam, and N. Neamati, ChemMedChem, 3, 986 (2008)
6 F. Aiello, A. Brizzi, A. Garofalo, F. Grande, G. Ragno, R. Dayam, and N. Neamati, Bioorgan. Med. Chem., 12, 4459 (2004)
7 N. Neamati, J.A. Turpin, H.E. Winslow, J.L. Christensen, K. Williamson, A. Orr, W.G. Rice, Y. Pommier, A. Garofalo, A. Brizzi, G. Campiani, I. Fiorini, and V. Nacci, J. Med. Chem., 42, 3334 (1999)
8 T. Suzuki, A. Matsuura, A. Kouketsu, S. Hisakawa, H. Nakagawa, and N. Miyata, Bioorgan. Med. Chem., 13, 4332 (2005)
9 A. Garofalo, G. Balconi, M. Botta, F. Corelli, M. DIncalci, G. Fabrizi, I. Fiorini, D. Lamba, and V. Nacci, Eur. J. Med. Chem., 28, 213 (1993)
10 T. Shiraiwa, T. Katayama, J. Ishikawa, T. Asai, and H. Kurokawa, Chem. Pharm. Bull., 47, 1180 (1999)
11 G. Bringmann, D. Feineis, and C. Hesselmann, Anal. Lett., 25, 497 (1992)
12 G. Bringmann, C. Hesselmann, and D. Feineis, J. Chromatogr., 595, 351 (1992)
13 Y.W. Zhang, S. Yao, H. Zeng, and H. Song, Curr. Pharm. Anal., 6, 114 (2010)
14 T. Radhakrishna, R.D. Sreenivas, K. Vyas, and G.O. Reddy, J. Pharm. Biomed. Anal., 22, 691 (2000)
15 V. Ravinder, S. Ashok, A.V.S.S. Prasad, G. Balaswamy, Y.R. Kumar, and B.V. Bhaskar, Chromatographia, 67, 331 (2008)
16 Y.W. Zhang, D. Yang, S. Yao, Y. Cao, and H. Song, J. Chromatogr. Sci., 49, 67 (2011)
17 L. Yu, H. Song, X. Xu, D. Yang, and Y.J. Chen, Chin. J. Anal. Chem., 35, 545 (2007)
18 Q. Sun and S.V. Olesik, J. Chromatogr. B, 745, 159 (2000)
19 G.S. Ding, Y. Liu, R.Z. Cong, and J.D. Wang, Talanta, 62, 997 (2004)
20 R.N. Rao, A.N. Raju, and D. Nagaraju, J. Pharm. Biomed. Anal., 42, 347 (2006)
21 A. Berthod, W. Li, and D.W. Armstrong, Anal. Chem., 64, 873 (1992)
22 B. Loun and D.S. Hage, Anal. Chem., 66, 3814 (1994)
23 R.W. Stringham and J.A. Blackwell, Anal. Chem., 69, 1414 (1997)
DOI :
Cytuj : Huo, H. L. ,Yu, S. H. ,Liu, X. Z. ,Meng, Y. ,Ren, Y. P. ,Zhang, L. T. ,Liu, L. ,Zhao, T. ,Cheng, X. M. ,Wang, C. H. ,Wang, Z. T. ,Gao, X. ,Jiang, X. ,Wang, L. ,Urban, J. ,Vaněk, J. ,Štys, D. ,Li, C. D. ,Liu, Y. ,Shi, Y. P. ,Zhang, Y. ,Peng, Q. ,Zeng, H. ,Yao, S. ,Zhang, Y. ,Song, H. , HPLC determination of enantiomeric thiazolidine-2-carboxylic acid on chiral stationary phase with pre-column derivatization. Acta Chromatographica Vol. 25, no. 2/2013
[Top]

RSM-optimized IRAE sample pretreatment and HPLC simultaneous determination of Tryptanthrin, indigo, and indirubin from Chinese herbal medicine Radix Isatidis

Czasopismo : Acta Chromatographica
Tytuł artykułu : RSM-optimized IRAE sample pretreatment and HPLC simultaneous determination of Tryptanthrin, indigo, and indirubin from Chinese herbal medicine Radix Isatidis

Autorzy :
Huo, H. L.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Yu, S. H.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Liu, X. Z.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Meng, Y.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Ren, Y. P.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Zhang, L. T.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China, zhanglantong@263.net,
Liu, L.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Zhao, T.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Cheng, X. M.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Wang, C. H.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China, wchcxm@hotmail.com,
Wang, Z. T.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China, wangzt@hotmail.com,
Gao, X.
Sichuan University Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy Chengdu, Sichuan China, gaomuxouzi@126.com,
Jiang, X.
Sichuan University Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy Chengdu, Sichuan China,
Wang, L.
Sichuan University Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy Chengdu, Sichuan China,
Urban, J.
University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, School of Complex Systems (former Institute of Physical Biology), Laboratory of Applied System Biology Zamek 136 Nove Hrady 373 33 Czech Republic, urbanj@frov.jcu.cz,
Vaněk, J.
University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, School of Complex Systems (former Institute of Physical Biology), Laboratory of Applied System Biology Zamek 136 Nove Hrady 373 33 Czech Republic,
Štys, D.
University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, School of Complex Systems (former Institute of Physical Biology), Laboratory of Applied System Biology Zamek 136 Nove Hrady 373 33 Czech Republic,
Li, C. D.
Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P.R. China,
Liu, Y.
Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P.R. China,
Shi, Y. P.
Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P.R. China, shiyp@licp.cas.cn,
Zhang, Y.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China, hangsong@vip.sina.com,
Peng, Q.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Zeng, H.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Yao, S.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Zhang, Y.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Song, H.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Zhou, W.
Guiyang Medical University Department of Pharmaceutical Analysis, School of Pharmacy Guiyang 550004 China,
Zhang, X. Y.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China, drxyzhang@126.com,
Lv, Y. P.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China,
Liu, X. D.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China,
Xu, C.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China,
Duan, G. L.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China, glduan@shmu.edu.cn,
Abstrakty : Radix Isatidis has widely useful activities including anti-virus, anti-bacterial. Tryptanthrin, indigo, and indirubin are active ingredients in R. Isatidis. Response surface methodology (RSM)-optimized infrared-assisted extraction (IRAE) was developed and combined with HPLC for simultaneous determination of tryptanthrin, indigo, and indirubin from R. Isatidis. IRAE were investigated through extraction yields of the three components and optimized by RSM. The optimum conditions were as follows: infrared power of 129 W, solid/liquid ratio of 1:40 g/mL, and irradiation time of 22.5 min. IRAE conditions obtained by RSM were not only accurate, but also had practical value reflecting the expected optimization. Subsequently, this novel IRAE method was evaluated by extraction yield of the components of R. Isatidis samples from different regions. Compared with common extraction methods including maceration extraction (ME), reflux extraction (RE), ultrasound-assisted extraction (UAE), and microwave-assisted extraction (MAE), IRAE showed higher yield with advantages of no limitation of solvent selection, low cost, convenience under optimum extraction conditions. These results suggested the potential of RSM-optimized IRAE for extraction and analysis of the water-/fat-soluble compositions of Chinese herbal medicine. A simple chromatographic separation for simultaneous determination of tryptanthrin, indigo, and indirubin from Chinese herbal medicine R. Isatidis was performed on a C18 column (Diamonsil 150 mm × 4.6 mm i.d., 5 μm) with a mobile phase isocratic consisting of methanol and water at a flow-rate of 0.8 mL min-1. The retention times of tryptanthrin, indigo, and indirubin were 15.4, 31.9, and 58.6 min, respectively. The linear equations were obtained as follows: y = −3094.5744 + 21208.792x for tryptanthrin (R = 0.9998, 0.9–18.0 μg mL-1), y = 4730.0448 + 30180.567x for indigo (R = 0.9997, 0.5–10.0 μg mL−1) and y = −6582.9045 + 67069.312x for indirubin (R = 0.9997, 0.4–8.0 μg mL-1). The result showed that RSM-optimized IRAE was a simple, efficient pretreatment method for the analysis of complex matrix.

Słowa kluczowe : Chinese herbal medicine, high-performance liquid chromatography, infrared-assisted extraction, Radix Isatidis, response surface methodology, tryptanthrin,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2013
Numer : Vol. 25, no. 2
Strony : 297 – 315
Bibliografia : 1 W.J. Kong, Y.L. Zhao, L.M. Shan, X.H. Xiao, and W.Y. Guo, J. Chromatogr. B, 871, 109–114 (2008)
2 W. Zhou, X.Y. Zhang, M.F. Xie, Y.L. Chen, Y. Li, and G.L. Duan, Chromatographia, 72, 719–724 (2010)
3 S.H. Nian and M. Liu, J. Huaihai Med., 23, 428–429 (2005)
4 C.L. Wang, J.L. Liu, and X.L. Shen, Chemistry Online, 70, 89–95 (2007)
5 L.F. Fan, L.T. Zhang, Z.F. Yuan, H.J. Xu, and W. He, Chin. J. Pharm. Anal., 28, 540–543 (2008)
6 J.E. Dong, M.G. Gong and Z.S. Liang, J. Northwest A&F Univ. (Nat Sci Ed), 35, 215–218 (2007)
7 H. Ni, X. Shi, and Y.H. Liu, J. Qiqihar Med. College, 29, 1610 (2008)
8 L.X. Sun, H. Tang, P. Yin, L.L. Ning, and K.S. Bi, J. Shenyang Pharm. Univ., 17, 191–192 (2000)
9 C.Y. Li and L.J. Zeng, Res. Prac. Chin. Med., 19, 51–54 (2005)
10 H. Sun, H.T. Lv, Y.M. Zhang, X.C. Meng, J.T. Sui, X.J. Wang, and K.S. Bi, J. Sep. Sci., 30, 3120–3126 (2007)
11 Y.F. Sha, S. Shen, and G.L. Duan, J. Pharm. Biomed. Anal., 36, 381–385 (2004)
12 B. Chen, Y.J. Yu, L. Gui, T. Zhou, and G.L. Duan, Fudan Univ. J. Med. Sci., 35, 142–144 (2008)
13 C.H. Deng, J. Ji, N. Li, Y.J. Yu, G.L. Duan, and X.M. Zhang, J. Chromatogr. A, 1117, 115–120 (2006)
14 Y.F. Sha, C.H. Deng, Z. Liu, T.M. Huang, B. Yang, and G.L. Duan, J. Chromatogr. B, 806, 271–276 (2004)
15 Y.J. Yu, T.M. Huang, B. Yang, X. Liu, and G.L. Duan, J. Pharm. Biomed. Anal., 43, 24–31 (2007)
16 X.L. Wang and L.Q. Yan, China Pharm., 18, 2119 (2007)
17 P. Zou, Y. Hong, and H.L. Koh, J. Pharm. Biomed. Anal., 38, 514–520 (2005)
DOI :
Cytuj : Huo, H. L. ,Yu, S. H. ,Liu, X. Z. ,Meng, Y. ,Ren, Y. P. ,Zhang, L. T. ,Liu, L. ,Zhao, T. ,Cheng, X. M. ,Wang, C. H. ,Wang, Z. T. ,Gao, X. ,Jiang, X. ,Wang, L. ,Urban, J. ,Vaněk, J. ,Štys, D. ,Li, C. D. ,Liu, Y. ,Shi, Y. P. ,Zhang, Y. ,Peng, Q. ,Zeng, H. ,Yao, S. ,Zhang, Y. ,Song, H. ,Zhou, W. ,Zhang, X. Y. ,Lv, Y. P. ,Liu, X. D. ,Xu, C. ,Duan, G. L. , RSM-optimized IRAE sample pretreatment and HPLC simultaneous determination of Tryptanthrin, indigo, and indirubin from Chinese herbal medicine Radix Isatidis. Acta Chromatographica Vol. 25, no. 2/2013
[Top]

Application of gas chromatography in the study of steam gasification and co-gasification of hard coal and biomass chars

Czasopismo : Acta Chromatographica
Tytuł artykułu : Application of gas chromatography in the study of steam gasification and co-gasification of hard coal and biomass chars

Autorzy :
Huo, H. L.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Yu, S. H.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Liu, X. Z.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Meng, Y.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Ren, Y. P.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Zhang, L. T.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China, zhanglantong@263.net,
Liu, L.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Zhao, T.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Cheng, X. M.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Wang, C. H.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China, wchcxm@hotmail.com,
Wang, Z. T.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China, wangzt@hotmail.com,
Gao, X.
Sichuan University Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy Chengdu, Sichuan China, gaomuxouzi@126.com,
Jiang, X.
Sichuan University Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy Chengdu, Sichuan China,
Wang, L.
Sichuan University Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy Chengdu, Sichuan China,
Urban, J.
University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, School of Complex Systems (former Institute of Physical Biology), Laboratory of Applied System Biology Zamek 136 Nove Hrady 373 33 Czech Republic, urbanj@frov.jcu.cz,
Vaněk, J.
University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, School of Complex Systems (former Institute of Physical Biology), Laboratory of Applied System Biology Zamek 136 Nove Hrady 373 33 Czech Republic,
Štys, D.
University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, School of Complex Systems (former Institute of Physical Biology), Laboratory of Applied System Biology Zamek 136 Nove Hrady 373 33 Czech Republic,
Li, C. D.
Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P.R. China,
Liu, Y.
Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P.R. China,
Shi, Y. P.
Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P.R. China, shiyp@licp.cas.cn,
Zhang, Y.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China, hangsong@vip.sina.com,
Peng, Q.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Zeng, H.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Yao, S.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Zhang, Y.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Song, H.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Zhou, W.
Guiyang Medical University Department of Pharmaceutical Analysis, School of Pharmacy Guiyang 550004 China,
Zhang, X. Y.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China, drxyzhang@126.com,
Lv, Y. P.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China,
Liu, X. D.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China,
Xu, C.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China,
Duan, G. L.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China, glduan@shmu.edu.cn,
Smoliński, A.
Central Mining Institute Department of Energy Saving and Air Protection Sq. Gwarkow 1 40-166 Katowice, Poland, smolin@gig.katowice.pl,
Howaniec, N.
Central Mining Institute Department of Energy Saving and Air Protection Sq. Gwarkow 1 40-166 Katowice, Poland,
Abstrakty : The process of steam co-gasification of hard coal and biomass is regarded as environment friendly alternative to classical fossil fuel combustion, especially in the light of continuous increase in energy demand and recognition of environmental concerns related to fossil fuel processing. In the paper, the results of experimental comparative study of steam gasification and co-gasification of hard coal and energy crop (Andropogon gerardii) biomass in a laboratory-scale fixed bed reactor at the temperatures of 700, 800, and 900°C are presented. The gas chromatography technique was used in analyses of concentrations of the main product gas components, that is, hydrogen, carbon monoxide, carbon dioxide, and methane. Some regularities in terms of variations between gasification and co-gasification product gas composition were observed, e.g., carbon monoxide content in biomass gasification and co-gasification product gas was on average approximately half the amount observed in hard coal gasification. Application of Fe2O3 and CaO in co-gasification led to increase in hydrogen content with simultaneous CO2 capture. Chemisorption of CO2 with CaO in the process of steam co-gasification was effective at 700°C and 800°C and resulted in average hydrogen content increase from 61 to 87%vol and from 62 to 76%vol at 700°C and 800°C, respectively, when compared to co-gasification without Fe2O3 and CaO.

Słowa kluczowe : gasification, coal, energy crop, biomass,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2013
Numer : Vol. 25, no. 2
Strony : 317 – 330
Bibliografia : 1 Agencja Rynku Energii (2011): Rynek Energii Elektrycznej, http://www.cire.pl/rynekenergii/podstawa.php?smid=207#produkcja
2 US Energy Information Administration (2010): International Energy Outlook 2010, EIA, Washington, www.eia.gov/oiaf/ieo/index.html
3 http://ec.europa.eu/energy/coal/sustainable_coal/ccs_en.htm
4 US National Energy Laboratory (2010): Gasification Technology Database, http://ww.netl.doe.gov/technologies/coalpower/gasification/worlddatabase/summary.html
5 http://www.fossil.energy.gov/programs/powersystems/cleancoal/
6 http://cordis.europa.eu/fp7/energy/home_en.html
7 http://www.hydrogen.energy.gov/
8 K. Kumabe, T. Hanaoka, S. Fujimoto, T. Minowa, and K. Sakanishi, Fuel, 86, 684 (2007)
9 K. Li, R. Zhang, and J. Bi, Int. J. Hydrogen Energ., 35, 2722 (2010)
10 M. Lapuerta, J.J. Hernández, A. Pazo, and J. López, Fuel, 89, 828 (2008)
11 J. Fermoso, B. Arias, M.V. Gil, M.G. Plaza, C. Pevida, J.J. Pis, and R. Rubiera, Bioresource Technol., 101, 3230 (2010)
12 A.G. Collot, Y. Zhuo, D.R. Dugwell, and R. Kandiyoti, Fuel, 79, 667 (1999)
13 K. Sjöström, G. Chen, Q. Yu, C. Brage, and C. Rosen, Fuel, 78, 1189 (1999)
14 Y.G. Pan, E. Velo, X. Roca, J.J. Manya, and L. Puigjaner, Fuel, 79, 1317 (2000)
15 J. Fermoso, B. Arias, M.G. Plaza, C. Pevida, F. Rubiera, J.J. Pis, F. García-Peña, and P. Casero, Fuel Process. Technol., 90, 926 (2009)
16 R.N. Andre, F. Pinto, C. Franco, M. Diasa, I. Gulyurtlu, M.A.A. Matos, and I. Cabrita, Fuel, 84, 1635 (2005)
17 L. Zhang, S. Xu, W. Zhao, and S. Liu, Fuel, 86, 353 (2007)
18 T. Sonobe, N. Worasuwannarak, and S. Pipatmanomai, Fuel Process. Technol., 89, 1371 (2008)
19 F. Pinto, H. Lopes, R.N. Andre, I. Gulyurtlu, and I. Cabrita, Fuel, 86, 2052 (2007)
20 R.C. Brown, O. Liua, and G. Norton, Biomass Bioenerg., 18, 499 (2000)
21 F. Pinto, C. Franco, H. Lopes, R.N. Andre, I. Gulyurtlu, and I. Cabrita, Fuel, 84, 2236 (2005)
22 J. Wang, M. Jiang, Y. Yao, Y. Zhang, and J. Cao, Fuel, 88, 1572 (2009)
23 A. Smoliński, Arch. Environ. Prot., 34, 23 (2008)
24 Smoliński, Energ. Conv. Manag., 52, 37 (2011)
25 Aigner, C. Pfeifer, and H. Hofbauer, Fuel, 90, 2404 (2011)
DOI :
Cytuj : Huo, H. L. ,Yu, S. H. ,Liu, X. Z. ,Meng, Y. ,Ren, Y. P. ,Zhang, L. T. ,Liu, L. ,Zhao, T. ,Cheng, X. M. ,Wang, C. H. ,Wang, Z. T. ,Gao, X. ,Jiang, X. ,Wang, L. ,Urban, J. ,Vaněk, J. ,Štys, D. ,Li, C. D. ,Liu, Y. ,Shi, Y. P. ,Zhang, Y. ,Peng, Q. ,Zeng, H. ,Yao, S. ,Zhang, Y. ,Song, H. ,Zhou, W. ,Zhang, X. Y. ,Lv, Y. P. ,Liu, X. D. ,Xu, C. ,Duan, G. L. ,Smoliński, A. ,Howaniec, N. , Application of gas chromatography in the study of steam gasification and co-gasification of hard coal and biomass chars. Acta Chromatographica Vol. 25, no. 2/2013
[Top]

Quantitative analysis of lycorine in endemic Galanthus xvalentinei nothosubsp. subplicatus by HPLC-DAD

Czasopismo : Acta Chromatographica
Tytuł artykułu : Quantitative analysis of lycorine in endemic Galanthus xvalentinei nothosubsp. subplicatus by HPLC-DAD

Autorzy :
Huo, H. L.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Yu, S. H.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Liu, X. Z.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Meng, Y.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Ren, Y. P.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Zhang, L. T.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China, zhanglantong@263.net,
Liu, L.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Zhao, T.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Cheng, X. M.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Wang, C. H.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China, wchcxm@hotmail.com,
Wang, Z. T.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China, wangzt@hotmail.com,
Gao, X.
Sichuan University Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy Chengdu, Sichuan China, gaomuxouzi@126.com,
Jiang, X.
Sichuan University Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy Chengdu, Sichuan China,
Wang, L.
Sichuan University Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy Chengdu, Sichuan China,
Urban, J.
University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, School of Complex Systems (former Institute of Physical Biology), Laboratory of Applied System Biology Zamek 136 Nove Hrady 373 33 Czech Republic, urbanj@frov.jcu.cz,
Vaněk, J.
University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, School of Complex Systems (former Institute of Physical Biology), Laboratory of Applied System Biology Zamek 136 Nove Hrady 373 33 Czech Republic,
Štys, D.
University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, School of Complex Systems (former Institute of Physical Biology), Laboratory of Applied System Biology Zamek 136 Nove Hrady 373 33 Czech Republic,
Li, C. D.
Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P.R. China,
Liu, Y.
Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P.R. China,
Shi, Y. P.
Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P.R. China, shiyp@licp.cas.cn,
Zhang, Y.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China, hangsong@vip.sina.com,
Peng, Q.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Zeng, H.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Yao, S.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Zhang, Y.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Song, H.
Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China,
Zhou, W.
Guiyang Medical University Department of Pharmaceutical Analysis, School of Pharmacy Guiyang 550004 China,
Zhang, X. Y.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China, drxyzhang@126.com,
Lv, Y. P.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China,
Liu, X. D.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China,
Xu, C.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China,
Duan, G. L.
Fudan University Department of Pharmaceutical Analysis, School of Pharmacy Shanghai 201203 China, glduan@shmu.edu.cn,
Smoliński, A.
Central Mining Institute Department of Energy Saving and Air Protection Sq. Gwarkow 1 40-166 Katowice, Poland, smolin@gig.katowice.pl,
Howaniec, N.
Central Mining Institute Department of Energy Saving and Air Protection Sq. Gwarkow 1 40-166 Katowice, Poland,
Unver-Somer, N.
Ege University Faculty of Pharmacy, Department of Pharmacognosy 35100 Bornova, Izmir Turkey,
Cicek-Polat, D.
Ege University Faculty of Pharmacy, Department of Pharmacognosy 35100 Bornova, Izmir Turkey,
Emir, A.
Ege University Faculty of Pharmacy, Department of Pharmacognosy 35100 Bornova, Izmir Turkey,
Onur, M. A.
Ege University Faculty of Pharmacy, Department of Pharmacognosy 35100 Bornova, Izmir Turkey,
Kaya, G. I.
Ege University Faculty of Pharmacy, Department of Pharmacognosy 35100 Bornova, Izmir Turkey,
Abstrakty : Galanthus xvalentinei (J. Allen) Beck nothosubsp. subplicatus (N. Zeybek) A. P. Davis (Amaryllidaceae) is an endemic hybrid between G. nivalis L. and G. plicatus M. Bieb. subsp. byzantinus (Baker) D. A. Webb. Lycorine, a common alkaloid found in Amaryllidaceae plants, has been shown to display important biological activities. In the present study, a reversed-phase high-performance liquid chromatographic method has been used for the quantitative determination of lycorine in the aerial parts and bulbs of G. xvalentinei nothosubsp. subplicatus. A simple method for the extraction of lycorine in lowmass plant samples was employed utilizing pre-packed columns with diatomaceous earth (Extrelut®). The chromatographic separation was carried out using an isocratic system with a mobile phase of trifluoroacetic acid-water-acetonitrile (0.01:95:5) applied at a flow rate of 1 mL min-1using diode array dedector. The content of lycorine in the bulbs of G. xvalentinei nothosubsp. subplicatus was found to be 0.0028%, however, it was not detected in the aerial parts of this hybrid.

Słowa kluczowe : Galanthus xvalentinei nothosubsp, subplicatus, Amarylidaceae, lycorine, HPLC,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2013
Numer : Vol. 25, no. 2
Strony : 331 – 338
Bibliografia : 1 A.P. Davis, in: M. Bishop, A.P. Davis and J. Grimshaw (eds.), Snowdrops. A Monograph of Cultivated Galanthus. Griffin Press Publishing Ltd., Cheltenham, 2006, pp. 9–11.
2 A.P. Davis, A. Byfield, N. Ozhatay, K. Taylor, Kew Bull. 56, 639 (2001)
3 O. Hoshino, in G.A. Cordell (ed) The Alkaloids Chemistry and Biology. Academic Press, New York, 1998, pp. 323–424
4 L. Szlávik, A. Gyuris, J. Minárovits, P. Forgo, J. Molnár, J. Hohmann, Planta Med., 70, 871 (2004)
5 B. Weniger, L. Italiano, J.P. Beck, J. Bastida, S. Bergaňon, C. Codina, A. Lobstein, R. Anton, Planta Med., 61, 77 (1995)
6 B. Sener, I. Orhan, J. Satajavivad, Phytother. Res., 17, 1220 (2003)
7 G.I. Kaya, A. Fillik, Y. Hısıl, N. Unver, Turkish J. Pharm. Sci., 1, 105 (2004)
8 B. Bozkurt Sarıkaya, N. Unver Somer, M.A. Onur, Turkish J. Pharm. Sci., 9, 2012 (in press)
9 G.I. Kaya, D. Cicek, B. Sarıkaya, M.A. Onur, N. Unver Somer, Nat. Prod. Commun., 5, 873 (2010)
10 G. Saltan Citoglu, B. Sever Yılmaz, O. Bahadır, Chem. Nat. Comp., 44, 826 (2008)
11 G.I. Kaya, N. Unver, B. Gözler, J. Bastida, Biochem. Syst. Ecol., 32, 1059 (2004)
12 N.R. Mustafa, I.K. Rhee, R. Verpoorte, J. Liq. Chromatogr., 26, 3217 (2003)
13 S. Berkov, J. Bastida, F. Viladomat, C. Codina, Phytochem. Anal., 19, 285 (2008)
DOI :
Cytuj : Huo, H. L. ,Yu, S. H. ,Liu, X. Z. ,Meng, Y. ,Ren, Y. P. ,Zhang, L. T. ,Liu, L. ,Zhao, T. ,Cheng, X. M. ,Wang, C. H. ,Wang, Z. T. ,Gao, X. ,Jiang, X. ,Wang, L. ,Urban, J. ,Vaněk, J. ,Štys, D. ,Li, C. D. ,Liu, Y. ,Shi, Y. P. ,Zhang, Y. ,Peng, Q. ,Zeng, H. ,Yao, S. ,Zhang, Y. ,Song, H. ,Zhou, W. ,Zhang, X. Y. ,Lv, Y. P. ,Liu, X. D. ,Xu, C. ,Duan, G. L. ,Smoliński, A. ,Howaniec, N. ,Unver-Somer, N. ,Cicek-Polat, D. ,Emir, A. ,Onur, M. A. ,Kaya, G. I. , Quantitative analysis of lycorine in endemic Galanthus xvalentinei nothosubsp. subplicatus by HPLC-DAD. Acta Chromatographica Vol. 25, no. 2/2013
[Top]