Category: Vol. 25, no. 4

Development and validation of HPTLC method for estimation of gymnemic acid in microencapsulated antidiabetic polyherbal formulations

Czasopismo : Acta Chromatographica
Tytuł artykułu : Development and validation of HPTLC method for estimation of gymnemic acid in microencapsulated antidiabetic polyherbal formulations

Autorzy :
Patil, A. N.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India,
Nirmal, S. A.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India, nirmalsunil@rediffmail.com,
Chavan, A. K.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India,
Abstrakty : Gymnemic acid (GA) is one of the phytoconstituents present in Gymnema sylvestre. Estimation of GA was carried out first time from microencapsulated polyherbal formulation. Microencapsulated polyherbal formulations (F1 and F2) contain various plant extracts; hence, proper resolution of GA peak in high-performance thin-layer liquid chromatography (HPTLC) analysis of F1 and F2 is the problem. Hence, HPTLC analysis method for F1 and F2 is developed and validated for quantitative determination of GA. HPTLC analysis of F1 and F2 was carried out using TLC aluminum plates precoated with silica gel 60F254 eluted with chloroform-methanol-water (6.5 mL + 4.5 mL + 1.0 mL), and densitometric analysis was carried out at 580 nm. Complete validation was performed using standard methods. This HPTLC method was found to be reproducible, accurate, and can detect GA at microgram level. The new optimized mobile phase gave good resolution of GA peak for its proper quantification in microencapsulated polyherbal formulation.

Słowa kluczowe : Gymnema sylvestre, gymnemic acid, HPTLC, microencapsulated polyherbal formulation, validation,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2013
Numer : Vol. 25, no. 4
Strony : 601 – 612
Bibliografia : 1 P.K. Mukherjee, Clin. Res. Reg. Affairs, 20, 249–264 (2003)
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DOI :
Cytuj : Patil, A. N. ,Nirmal, S. A. ,Chavan, A. K. , Development and validation of HPTLC method for estimation of gymnemic acid in microencapsulated antidiabetic polyherbal formulations. Acta Chromatographica Vol. 25, no. 4/2013

Optimization and validation of a method based on derivatization with methylating agent followed by HPLC-DAD for determining dithiocarbamates residues

Czasopismo : Acta Chromatographica
Tytuł artykułu : Optimization and validation of a method based on derivatization with methylating agent followed by HPLC-DAD for determining dithiocarbamates residues

Autorzy :
Patil, A. N.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India,
Nirmal, S. A.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India, nirmalsunil@rediffmail.com,
Chavan, A. K.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India,
Zhou, L.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Xu, J.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Luan, L.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Ma, J.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Gong, Y.
Ministry of Agriculture Institute for the Control of Agrochemicals Beijing 100026 China,
Qin, D.
Ministry of Agriculture Institute for the Control of Agrochemicals Beijing 100026 China,
Pan, C.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Abstrakty : Dithiocarbamates fungicides (DTCs) are worldwidely used fungicides. Residue analytical methods on DTCs are usually based on headspace gas chromatography, which are not much stable and precise. In this study, a specific, simple and reliable method for determining DTCs fungicides residues was optimized and validated. The DTCs in foods and soils were extracted with an alkaline solution of EDTA and L-cysteine, followed by pH adjusting and methyl derivatization in methyl iodine solution. The organic layer of the reactants was separated, concentrated under vacuum and reconstructed in acetonitrile. DTCs residues were eluted on a C18 column and detected by HPLC-DAD at 272 nm. The S-alkyl derivatives of thiram, mancozeb and propineb were separated at different retention times. At fortified levels of 0.05 mg/kg to 2 mg/kg (residue expressed as CS2, in mg/kg, the same below), it is found that recoveries for DTCs spiked in apple, cucumber, tomato, rice and soil samples ranged from 70.8% to 105.3%, with relative standard deviations (RSD) from 0.6% to 13.7%. Limits of detection (LODs) and quantification (LOQs) ranged from 0.003 to 0.026 mg/kg and from 0.011 to 0.105 mg/kg for various foods and soils. This method was also applied to real sample tests.

Słowa kluczowe : dithiocarbamates, methyl derivatization, pesticide residue, HPLC,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2013
Numer : Vol. 25, no. 4
Strony : 613 – 625
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DOI :
Cytuj : Patil, A. N. ,Nirmal, S. A. ,Chavan, A. K. ,Zhou, L. ,Xu, J. ,Luan, L. ,Ma, J. ,Gong, Y. ,Qin, D. ,Pan, C. , Optimization and validation of a method based on derivatization with methylating agent followed by HPLC-DAD for determining dithiocarbamates residues. Acta Chromatographica Vol. 25, no. 4/2013
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Determination of four kinds of endogenous hormones in poplar dialyzate by HPLC with microdialysis

Czasopismo : Acta Chromatographica
Tytuł artykułu : Determination of four kinds of endogenous hormones in poplar dialyzate by HPLC with microdialysis

Autorzy :
Patil, A. N.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India,
Nirmal, S. A.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India, nirmalsunil@rediffmail.com,
Chavan, A. K.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India,
Zhou, L.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Xu, J.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Luan, L.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Ma, J.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Gong, Y.
Ministry of Agriculture Institute for the Control of Agrochemicals Beijing 100026 China,
Qin, D.
Ministry of Agriculture Institute for the Control of Agrochemicals Beijing 100026 China,
Pan, C.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Jinlong, M.
Zhejiang University Department of Chemical and Biological Engineering Hangzhou 310027 China,
Guobin, J.
Dalian Nationalities University Environment and Resources College Dalian 11660 China,
Bo, J.
Dalian Nationalities University Life Science College Dalian 116600 China,
Hua, J.
Zhejiang University Department of Chemical and Biological Engineering Hangzhou 310027 China,
Abstrakty : A dialyzate was obtained from the tender stems of living poplar (Populus wutunensis) by microdialysis (MD). Four kinds of endogenous hormones, namely, benzylamino purine, gibberellic acid, indole acetic acid, and abscisic acid, were then simultaneously determined by HPLC. The molecular weight cutoff (MWCO) of the MD probe was 30 kDa. The perfusion rate was 1 μL/min, and the perfusion liquid was Millipore-Q water. HPLC was performed using an Inertsil ODS-SP column. The mobile phase comprised 60:40 (v/v) methanol and water containing 0.2% phosphoric acid. The elution rate was 0.6 mL/min rate at 30°C. The average recoveries of the four kinds of hormones ranged from 93% to 109%, with correlation coefficients greater than 0.998. The determinations of endogenous hormones in plants as real time, living, and online were realized by MD-HPLC. The cells and macromolecules were excluded by the MD probe so centrifugation or protein precipitation was not required prior to analysis. MD-HPLC is a highly sensitive and selective method for the simultaneous profiling and quantification of a wide variety of plant hormone groups. This method is convenient for studying the mechanism of plant hormones.

Słowa kluczowe : microdialysis, high-performance liquid chromatography, Populus wutunensis, endogenous hormones,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2013
Numer : Vol. 25, no. 4
Strony : 627 – 637
Bibliografia : 1 P.J. Davies, in P.J. Davies (ed) Plant Hormones: Physiology, Biochemistry and Molecular Biology, Kluwer, Boston, 1995, pp. 13–38
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DOI :
Cytuj : Patil, A. N. ,Nirmal, S. A. ,Chavan, A. K. ,Zhou, L. ,Xu, J. ,Luan, L. ,Ma, J. ,Gong, Y. ,Qin, D. ,Pan, C. ,Jinlong, M. ,Guobin, J. ,Bo, J. ,Hua, J. , Determination of four kinds of endogenous hormones in poplar dialyzate by HPLC with microdialysis. Acta Chromatographica Vol. 25, no. 4/2013
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Development and validation of a reversed-phase liquid chromatography method for the quantification of meloxicam in liposomes

Czasopismo : Acta Chromatographica
Tytuł artykułu : Development and validation of a reversed-phase liquid chromatography method for the quantification of meloxicam in liposomes

Autorzy :
Patil, A. N.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India,
Nirmal, S. A.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India, nirmalsunil@rediffmail.com,
Chavan, A. K.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India,
Zhou, L.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Xu, J.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Luan, L.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Ma, J.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Gong, Y.
Ministry of Agriculture Institute for the Control of Agrochemicals Beijing 100026 China,
Qin, D.
Ministry of Agriculture Institute for the Control of Agrochemicals Beijing 100026 China,
Pan, C.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Jinlong, M.
Zhejiang University Department of Chemical and Biological Engineering Hangzhou 310027 China,
Guobin, J.
Dalian Nationalities University Environment and Resources College Dalian 11660 China,
Bo, J.
Dalian Nationalities University Life Science College Dalian 116600 China,
Hua, J.
Zhejiang University Department of Chemical and Biological Engineering Hangzhou 310027 China,
Montejo, C.
Universidad CEU San Pablo Departamento de Ciencias Farmacéuticas y de la Alimentación, Área de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28668 Madrid Spain, montejo@ceu.es,
Civera, C.
Universidad Complutense de Madrid Departamento de Química Física II, Facultad de Farmacia 28040 Madrid Spain,
Barcia, E.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Negro, S.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Fernández-Carballido, A.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Abstrakty : In the present study, we have developed and validated an analytical method for the determination of meloxicam in liposomes using high-performance liquid chromatography-ultraviolet (HPLC-UV). Chromatographic separation was carried out on an Ascentis RP amide C16 column selecting a mobile phase composed of acetonitrile-0.3% formic acid solution (40:60, v/v) adjusted at pH 2.8. The mobile phase flow rate selected was 0.5 mL min-1and UV detection at 355 nm. Piroxicam was chosen as internal standard. All the analyses were performed at temperatures of 40.0 ± 0.5°C. The calibration curve was linear over the range 18–420 ng mL-1. Relative standard deviation (RSD) for precision was <1.03%. Accuracy ranged between 98.53% and 101.41% with RSD lower than 1.5%. LOD and LOQ were 5 ng mL-1and 15 ng mL-1, respectively. The method was simple, rapid, and easy to apply, making it very suitable for routine analysis of meloxicam in liposomes. The method could also be used with reliability for the determination of meloxicam in other pharmaceutical dosage forms.

Słowa kluczowe : HPLC, liposomes, meloxicam, UV detection,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2013
Numer : Vol. 25, no. 4
Strony : 639 – 653
Bibliografia : 1 R.N. Rao, S. Meena, and A.R. Rao, J. Pharm. Biomed. Anal., 39, 349–363 (2005)
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DOI :
Cytuj : Patil, A. N. ,Nirmal, S. A. ,Chavan, A. K. ,Zhou, L. ,Xu, J. ,Luan, L. ,Ma, J. ,Gong, Y. ,Qin, D. ,Pan, C. ,Jinlong, M. ,Guobin, J. ,Bo, J. ,Hua, J. ,Montejo, C. ,Civera, C. ,Barcia, E. ,Negro, S. ,Fernández-Carballido, A. , Development and validation of a reversed-phase liquid chromatography method for the quantification of meloxicam in liposomes. Acta Chromatographica Vol. 25, no. 4/2013
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The sensitive and simple measurement of underivatized cholesterol and its oxygen derivatives in biological materials by capillary gas chromatography coupled to a mass-selective detector

Czasopismo : Acta Chromatographica
Tytuł artykułu : The sensitive and simple measurement of underivatized cholesterol and its oxygen derivatives in biological materials by capillary gas chromatography coupled to a mass-selective detector

Autorzy :
Patil, A. N.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India,
Nirmal, S. A.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India, nirmalsunil@rediffmail.com,
Chavan, A. K.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India,
Zhou, L.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Xu, J.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Luan, L.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Ma, J.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Gong, Y.
Ministry of Agriculture Institute for the Control of Agrochemicals Beijing 100026 China,
Qin, D.
Ministry of Agriculture Institute for the Control of Agrochemicals Beijing 100026 China,
Pan, C.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Jinlong, M.
Zhejiang University Department of Chemical and Biological Engineering Hangzhou 310027 China,
Guobin, J.
Dalian Nationalities University Environment and Resources College Dalian 11660 China,
Bo, J.
Dalian Nationalities University Life Science College Dalian 116600 China,
Hua, J.
Zhejiang University Department of Chemical and Biological Engineering Hangzhou 310027 China,
Montejo, C.
Universidad CEU San Pablo Departamento de Ciencias Farmacéuticas y de la Alimentación, Área de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28668 Madrid Spain, montejo@ceu.es,
Civera, C.
Universidad Complutense de Madrid Departamento de Química Física II, Facultad de Farmacia 28040 Madrid Spain,
Barcia, E.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Negro, S.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Fernández-Carballido, A.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Czauderna, M.
Polish Academy of Sciences The Kielanowski Institute of Animal Physiology and Nutrition 05-110 Jabłonna Poland, mr.czauderna@gmail.com,
Marounek, M.
Prague-Uhříněves The Institute of Animal Science Prague Czech Republic,
Duskova, D.
Prague-Uhříněves The Institute of Animal Science Prague Czech Republic,
Kowalczyk, J.
Polish Academy of Sciences The Kielanowski Institute of Animal Physiology and Nutrition 05-110 Jabłonna Poland,
Abstrakty : A method for determining underivatized cholesterol and its oxygen derivatives (ChlOs) in animal tissue specimens and some food has been developed using capillary gas chromatography (GC) and mass spectrometry (MS) with 5α-cholestane as the internal standard. The analysis preparation procedure for cholesterol and ChlOs consisted of lipid extraction followed by gentle saponification using 2 M KOH in ethanol. The analyzed samples were saponified overnight at room temperature in the dark with shaking. Then, free cholesterol and ChlOs were extracted using diethyl ether. The extracts were dried under argon, and the residues were redissolved in chloroform before GC-MS analysis. Cholesterol was analyzed in biological materials using the short column temperature program. The simultaneous quantification of cholesterol and its oxides in biological materials was performed using the longer column temperature program. Cholesterol was eluted faster than ChlOs. The chromatographic method that used column temperature program A can be successfully used for the simple and rapid quantification of cholesterol in biological materials, while the method using column temperature program B is more suitable for the simultaneous quantification of cholesterol and its oxides in biological materials.

Słowa kluczowe : cholesterol, oxygen derivatives of cholesterol, gas chromatography, mass spectrometry, biological material,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2013
Numer : Vol. 25, no. 4
Strony : 655 – 667
Bibliografia : 1 G. Leonarduzzi, B. Sottero, G. Poli, J. Nutr. Biochem., 13, 700 (2002)
2 M. Pieszka, Pol. J. Food Nutr. Sci., 57, 509 (2007)
3 M. Czauderna, J. Kowalczyk, K.M. Niedźwiedzka, Chem. Anal. (Warsaw), 53, 203 (2009)
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DOI :
Cytuj : Patil, A. N. ,Nirmal, S. A. ,Chavan, A. K. ,Zhou, L. ,Xu, J. ,Luan, L. ,Ma, J. ,Gong, Y. ,Qin, D. ,Pan, C. ,Jinlong, M. ,Guobin, J. ,Bo, J. ,Hua, J. ,Montejo, C. ,Civera, C. ,Barcia, E. ,Negro, S. ,Fernández-Carballido, A. ,Czauderna, M. ,Marounek, M. ,Duskova, D. ,Kowalczyk, J. , The sensitive and simple measurement of underivatized cholesterol and its oxygen derivatives in biological materials by capillary gas chromatography coupled to a mass-selective detector. Acta Chromatographica Vol. 25, no. 4/2013
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Development and validation of a sensitive LC-MS/MS method for determination of valacyclovir in human plasma: Application to a bioequivalence study

Czasopismo : Acta Chromatographica
Tytuł artykułu : Development and validation of a sensitive LC-MS/MS method for determination of valacyclovir in human plasma: Application to a bioequivalence study

Autorzy :
Patil, A. N.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India,
Nirmal, S. A.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India, nirmalsunil@rediffmail.com,
Chavan, A. K.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India,
Zhou, L.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Xu, J.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Luan, L.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Ma, J.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Gong, Y.
Ministry of Agriculture Institute for the Control of Agrochemicals Beijing 100026 China,
Qin, D.
Ministry of Agriculture Institute for the Control of Agrochemicals Beijing 100026 China,
Pan, C.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Jinlong, M.
Zhejiang University Department of Chemical and Biological Engineering Hangzhou 310027 China,
Guobin, J.
Dalian Nationalities University Environment and Resources College Dalian 11660 China,
Bo, J.
Dalian Nationalities University Life Science College Dalian 116600 China,
Hua, J.
Zhejiang University Department of Chemical and Biological Engineering Hangzhou 310027 China,
Montejo, C.
Universidad CEU San Pablo Departamento de Ciencias Farmacéuticas y de la Alimentación, Área de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28668 Madrid Spain, montejo@ceu.es,
Civera, C.
Universidad Complutense de Madrid Departamento de Química Física II, Facultad de Farmacia 28040 Madrid Spain,
Barcia, E.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Negro, S.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Fernández-Carballido, A.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Czauderna, M.
Polish Academy of Sciences The Kielanowski Institute of Animal Physiology and Nutrition 05-110 Jabłonna Poland, mr.czauderna@gmail.com,
Marounek, M.
Prague-Uhříněves The Institute of Animal Science Prague Czech Republic,
Duskova, D.
Prague-Uhříněves The Institute of Animal Science Prague Czech Republic,
Kowalczyk, J.
Polish Academy of Sciences The Kielanowski Institute of Animal Physiology and Nutrition 05-110 Jabłonna Poland,
Konda, R. K.
Hindu College of Pharmacy Department of Pharmaceutical Chemistry Amaravathi Road Guntur Andhrapradesh 522002 India, victory2ravi@yahoo.co.in,
Chandu, B. R.
Donbosco College of Pharmacy Department of Pharmaceutical Sciences Pulladigunta Guntur 522201 India,
Challa, B. R.
Nirmala College of Pharmacy Department of Pharmaceutical Analysis Madras Road Kadapa Andhrapradesh 516002 India, baluchalla_99@yahoo.com,
Chandrasekhar, K. B.
Jawaharlal Nehru Technological University Department of Chemistry Anantapur 515002 India,
Abstrakty : A simple, sensitive, and highly specific method has been developed for determination of valacyclovir (VL) in human plasma. The analytical procedure involves a solid-phase extraction method using Valacyclovir-D8 (VLD8) as an internal standard. Chromatographic separation was carried out on a reversed phase Zorbax, SB C18, 4.6 × 75 mm, 3.5μm column. Valacyclovir and Valacyclovir-D8 were detected with proton adducts at m/z 325.2 → 152.0 and 333.3 → 152.0 in multiple reaction monitoring (MRM) positive mode. The method was linear over the concentration range of 0.5–700.0 ng mL-1. The limit of detection (LOD) and limit of quantification (LOQ) for valacyclovir were 0.2 pg mL-1and 0.5 ng mL-1, respectively. The method was shown to be precise with the average within-run and between-run variations of 0.7 to 3.5% and 3.1 to 4.7%, respectively. The average within-run and between-run accuracies of the method throughout its linear range were 96.7 to 97.9 and 94.7 to 97.3%, respectively. The mean recoveries of valacyclovir and Valacyclovir-D8 from human plasma by the developed method were 99.17 ± 10.78% and 110.84 ± 8.74%, respectively. The method was successfully applied in bioequivalence study with 20 healthy male volunteers under fasting condition.

Słowa kluczowe : valacyclovir, LC-MS/MS, solid-phase extraction, bioequivalence,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2013
Numer : Vol. 25, no. 4
Strony : 669 – 686
Bibliografia : 1 B.S. Anand, S. Katragadda, and Ashim K. Mitra, JPET, 311, 659 (2004)
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DOI :
Cytuj : Patil, A. N. ,Nirmal, S. A. ,Chavan, A. K. ,Zhou, L. ,Xu, J. ,Luan, L. ,Ma, J. ,Gong, Y. ,Qin, D. ,Pan, C. ,Jinlong, M. ,Guobin, J. ,Bo, J. ,Hua, J. ,Montejo, C. ,Civera, C. ,Barcia, E. ,Negro, S. ,Fernández-Carballido, A. ,Czauderna, M. ,Marounek, M. ,Duskova, D. ,Kowalczyk, J. ,Konda, R. K. ,Chandu, B. R. ,Challa, B. R. ,Chandrasekhar, K. B. , Development and validation of a sensitive LC-MS/MS method for determination of valacyclovir in human plasma: Application to a bioequivalence study. Acta Chromatographica Vol. 25, no. 4/2013
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Isolation of gallic acid and methyl gallate from folium Toonea Sinensis and validated method for their quantitation using LC-based technologies

Czasopismo : Acta Chromatographica
Tytuł artykułu : Isolation of gallic acid and methyl gallate from folium Toonea Sinensis and validated method for their quantitation using LC-based technologies

Autorzy :
Patil, A. N.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India,
Nirmal, S. A.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India, nirmalsunil@rediffmail.com,
Chavan, A. K.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India,
Zhou, L.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Xu, J.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Luan, L.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Ma, J.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Gong, Y.
Ministry of Agriculture Institute for the Control of Agrochemicals Beijing 100026 China,
Qin, D.
Ministry of Agriculture Institute for the Control of Agrochemicals Beijing 100026 China,
Pan, C.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Jinlong, M.
Zhejiang University Department of Chemical and Biological Engineering Hangzhou 310027 China,
Guobin, J.
Dalian Nationalities University Environment and Resources College Dalian 11660 China,
Bo, J.
Dalian Nationalities University Life Science College Dalian 116600 China,
Hua, J.
Zhejiang University Department of Chemical and Biological Engineering Hangzhou 310027 China,
Montejo, C.
Universidad CEU San Pablo Departamento de Ciencias Farmacéuticas y de la Alimentación, Área de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28668 Madrid Spain, montejo@ceu.es,
Civera, C.
Universidad Complutense de Madrid Departamento de Química Física II, Facultad de Farmacia 28040 Madrid Spain,
Barcia, E.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Negro, S.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Fernández-Carballido, A.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Czauderna, M.
Polish Academy of Sciences The Kielanowski Institute of Animal Physiology and Nutrition 05-110 Jabłonna Poland, mr.czauderna@gmail.com,
Marounek, M.
Prague-Uhříněves The Institute of Animal Science Prague Czech Republic,
Duskova, D.
Prague-Uhříněves The Institute of Animal Science Prague Czech Republic,
Kowalczyk, J.
Polish Academy of Sciences The Kielanowski Institute of Animal Physiology and Nutrition 05-110 Jabłonna Poland,
Konda, R. K.
Hindu College of Pharmacy Department of Pharmaceutical Chemistry Amaravathi Road Guntur Andhrapradesh 522002 India, victory2ravi@yahoo.co.in,
Chandu, B. R.
Donbosco College of Pharmacy Department of Pharmaceutical Sciences Pulladigunta Guntur 522201 India,
Challa, B. R.
Nirmala College of Pharmacy Department of Pharmaceutical Analysis Madras Road Kadapa Andhrapradesh 516002 India, baluchalla_99@yahoo.com,
Chandrasekhar, K. B.
Jawaharlal Nehru Technological University Department of Chemistry Anantapur 515002 India,
Shen, Y.
Jiangsu University School of Pharmacy Zhenjiang China,
Yang, H.
Jiangsu University School of Pharmacy Zhenjiang China,
Xia, G.
Jiangsu University School of Pharmacy Zhenjiang China,
Wang, J.
Jiangsu University School of Pharmacy Zhenjiang China,
Cai, B.
Nanjing University of Chinese Medicine Jiangsu Key Laboratory of Chinese Medicine Processing, SATCM Key Laboratory of Standardization for Chinese Medicine Processing Nanjing China,
Jia, X.
Jiangsu University School of Pharmacy Zhenjiang China, jxiaobin2005@hotmail.com,
Abstrakty : Silica-gel column chromatography and preparative reversed-phase high-performance liquid chromatography (RP-HPLC) were sequentially employed for the isolation of two antioxidants including gallic acid and methyl gallate from Folium Toonea Sinensis. An RP-HPLC-UV method was then developed and validated to rapidly determine their content in this herb with ethyl gallate as internal standard. The quantitation was performed on an XBridge Shield RP18 column (150 mm × 4.6 mm, 5 μm) under 40°C. The mobile phase consisting of acetonitrile and 0.1% formic acid aqueous solution was driven at 1.0 mL min-1under gradient elution, and 270 nm was selected to monitor the separation. To evaluate the fitness for purpose of the method and to investigate the difference in the content of analytes among different samples, the leaves collected from five production sites were analyzed. The newly established method is suitable for routine analysis of gallic acid and methyl gallate in the herb and, hence, can assist in its quality assessment. It was also found that not only the content of two antioxidants but also the ratio varied significantly among different geographical origins. In addition, three samples from Yantai, Zumadian, and Zhenjiang were distinguished as they have a much higher content ratio than the other two.

Słowa kluczowe : Toona sinensis, Folium Toonea Sinensis, RP-HPLC-UV, gallic acid, methyl gallate,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2013
Numer : Vol. 25, no. 4
Strony : 687 – 701
Bibliografia : 1 Y.C. Hseu, W.H. Chang, C.S. Chen, J.W. Liao, C.J. Huang, and F.J. Lu, Food Chem. Toxicol., 1, 105 (2008)
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DOI :
Cytuj : Patil, A. N. ,Nirmal, S. A. ,Chavan, A. K. ,Zhou, L. ,Xu, J. ,Luan, L. ,Ma, J. ,Gong, Y. ,Qin, D. ,Pan, C. ,Jinlong, M. ,Guobin, J. ,Bo, J. ,Hua, J. ,Montejo, C. ,Civera, C. ,Barcia, E. ,Negro, S. ,Fernández-Carballido, A. ,Czauderna, M. ,Marounek, M. ,Duskova, D. ,Kowalczyk, J. ,Konda, R. K. ,Chandu, B. R. ,Challa, B. R. ,Chandrasekhar, K. B. ,Shen, Y. ,Yang, H. ,Xia, G. ,Wang, J. ,Cai, B. ,Jia, X. , Isolation of gallic acid and methyl gallate from folium Toonea Sinensis and validated method for their quantitation using LC-based technologies. Acta Chromatographica Vol. 25, no. 4/2013
[Top]

HPLC analysis in drug level monitoring of K027

Czasopismo : Acta Chromatographica
Tytuł artykułu : HPLC analysis in drug level monitoring of K027

Autorzy :
Patil, A. N.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India,
Nirmal, S. A.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India, nirmalsunil@rediffmail.com,
Chavan, A. K.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India,
Zhou, L.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Xu, J.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Luan, L.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Ma, J.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Gong, Y.
Ministry of Agriculture Institute for the Control of Agrochemicals Beijing 100026 China,
Qin, D.
Ministry of Agriculture Institute for the Control of Agrochemicals Beijing 100026 China,
Pan, C.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Jinlong, M.
Zhejiang University Department of Chemical and Biological Engineering Hangzhou 310027 China,
Guobin, J.
Dalian Nationalities University Environment and Resources College Dalian 11660 China,
Bo, J.
Dalian Nationalities University Life Science College Dalian 116600 China,
Hua, J.
Zhejiang University Department of Chemical and Biological Engineering Hangzhou 310027 China,
Montejo, C.
Universidad CEU San Pablo Departamento de Ciencias Farmacéuticas y de la Alimentación, Área de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28668 Madrid Spain, montejo@ceu.es,
Civera, C.
Universidad Complutense de Madrid Departamento de Química Física II, Facultad de Farmacia 28040 Madrid Spain,
Barcia, E.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Negro, S.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Fernández-Carballido, A.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Czauderna, M.
Polish Academy of Sciences The Kielanowski Institute of Animal Physiology and Nutrition 05-110 Jabłonna Poland, mr.czauderna@gmail.com,
Marounek, M.
Prague-Uhříněves The Institute of Animal Science Prague Czech Republic,
Duskova, D.
Prague-Uhříněves The Institute of Animal Science Prague Czech Republic,
Kowalczyk, J.
Polish Academy of Sciences The Kielanowski Institute of Animal Physiology and Nutrition 05-110 Jabłonna Poland,
Konda, R. K.
Hindu College of Pharmacy Department of Pharmaceutical Chemistry Amaravathi Road Guntur Andhrapradesh 522002 India, victory2ravi@yahoo.co.in,
Chandu, B. R.
Donbosco College of Pharmacy Department of Pharmaceutical Sciences Pulladigunta Guntur 522201 India,
Challa, B. R.
Nirmala College of Pharmacy Department of Pharmaceutical Analysis Madras Road Kadapa Andhrapradesh 516002 India, baluchalla_99@yahoo.com,
Chandrasekhar, K. B.
Jawaharlal Nehru Technological University Department of Chemistry Anantapur 515002 India,
Shen, Y.
Jiangsu University School of Pharmacy Zhenjiang China,
Yang, H.
Jiangsu University School of Pharmacy Zhenjiang China,
Xia, G.
Jiangsu University School of Pharmacy Zhenjiang China,
Wang, J.
Jiangsu University School of Pharmacy Zhenjiang China,
Cai, B.
Nanjing University of Chinese Medicine Jiangsu Key Laboratory of Chinese Medicine Processing, SATCM Key Laboratory of Standardization for Chinese Medicine Processing Nanjing China,
Jia, X.
Jiangsu University School of Pharmacy Zhenjiang China, jxiaobin2005@hotmail.com,
Nurulain, S. M.
United Arab Emirates University Department of Pharmacology and Pharmacotherapy Al Ain United Arab Emirates,
Kalász, H.
Semmelweis University Department of Pharmacology and Pharmacotherapy Budapest Hungary, huba.kalasz@gmail.com,
Szegi, P.
Semmelweis University Department of Pharmacodynamics Budapest Hungary,
Kuca, K.
University Hospital Biomedical Research Center Hradec Kralove Czech Republic,
Adem, A.
United Arab Emirates University Department of Pharmacology and Pharmacotherapy Al Ain United Arab Emirates,
Hasan, M. Y. B.
United Arab Emirates University Department of Pharmacology and Pharmacotherapy Al Ain United Arab Emirates,
Hashemi, F.
Semmelweis University Department of Pharmacodynamics Budapest Hungary,
Tekes, K.
Semmelweis University Department of Pharmacodynamics Budapest Hungary,
Abstrakty : HPLC monitoring of pharmacokinetics was done in two body compartments of rats following intramuscular treatments with three different doses of K027, a bispyridinium mono-aldoxime type of antidotes to organoposphate intoxicated subjects. Reversed-phase HPLC separation with electrochemical detections was done to monitor the onset, the maximum level, and offset in K027 concentration. Serum level of K027 showed a fast onset, independently from the doses of K027. Drug level in brain was showing an essentially delayed kinetics.

Słowa kluczowe : HPLC, K027, pharmacokinetics, blood-brain barrier, reversed-phase separation, quantitation,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2013
Numer : Vol. 25, no. 4
Strony : 703 – 710
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18 H. Kalász, M.Y. Hasan, R. Sheen, K. Kuca, G. Petroianu, K. Ludányi, A. Gergely, and K. Tekes, Anal. Bioanal. Chem., 385, 1062–1067 (2006)
19 K. Musilek, D. Jun, J. Cabal, J. Kassa, F. Gunn-Moore, and K. Kuca, J. Med. Chem., 50, 5514–5518 (2007)
20 K. Sakurada, K. Matsubara, K. Shimizu, H. Shiono, Y. Seto, K. Tsuge, M. Yoshino, I. Sakai, H. Mukoyama, and T. Takatori, Neurochem. Res., 28, 1401–1407 (2003)
21 S. Okuno, K. Sakurada, H. Ohta, H. Ikegaya, Y. Kazui, T. Akutsu, T. Takatori, and K. Iwadate, Toxicol. Appl. Pharmacol., 227, 8–15 (2008)
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DOI :
Cytuj : Patil, A. N. ,Nirmal, S. A. ,Chavan, A. K. ,Zhou, L. ,Xu, J. ,Luan, L. ,Ma, J. ,Gong, Y. ,Qin, D. ,Pan, C. ,Jinlong, M. ,Guobin, J. ,Bo, J. ,Hua, J. ,Montejo, C. ,Civera, C. ,Barcia, E. ,Negro, S. ,Fernández-Carballido, A. ,Czauderna, M. ,Marounek, M. ,Duskova, D. ,Kowalczyk, J. ,Konda, R. K. ,Chandu, B. R. ,Challa, B. R. ,Chandrasekhar, K. B. ,Shen, Y. ,Yang, H. ,Xia, G. ,Wang, J. ,Cai, B. ,Jia, X. ,Nurulain, S. M. ,Kalász, H. ,Szegi, P. ,Kuca, K. ,Adem, A. ,Hasan, M. Y. B. ,Hashemi, F. ,Tekes, K. , HPLC analysis in drug level monitoring of K027. Acta Chromatographica Vol. 25, no. 4/2013
[Top]

Stress degradation studies on aliskiren and the development of a sensitive stability-indicating MEKC method

Czasopismo : Acta Chromatographica
Tytuł artykułu : Stress degradation studies on aliskiren and the development of a sensitive stability-indicating MEKC method

Autorzy :
Patil, A. N.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India,
Nirmal, S. A.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India, nirmalsunil@rediffmail.com,
Chavan, A. K.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India,
Zhou, L.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Xu, J.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Luan, L.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Ma, J.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Gong, Y.
Ministry of Agriculture Institute for the Control of Agrochemicals Beijing 100026 China,
Qin, D.
Ministry of Agriculture Institute for the Control of Agrochemicals Beijing 100026 China,
Pan, C.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Jinlong, M.
Zhejiang University Department of Chemical and Biological Engineering Hangzhou 310027 China,
Guobin, J.
Dalian Nationalities University Environment and Resources College Dalian 11660 China,
Bo, J.
Dalian Nationalities University Life Science College Dalian 116600 China,
Hua, J.
Zhejiang University Department of Chemical and Biological Engineering Hangzhou 310027 China,
Montejo, C.
Universidad CEU San Pablo Departamento de Ciencias Farmacéuticas y de la Alimentación, Área de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28668 Madrid Spain, montejo@ceu.es,
Civera, C.
Universidad Complutense de Madrid Departamento de Química Física II, Facultad de Farmacia 28040 Madrid Spain,
Barcia, E.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Negro, S.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Fernández-Carballido, A.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Czauderna, M.
Polish Academy of Sciences The Kielanowski Institute of Animal Physiology and Nutrition 05-110 Jabłonna Poland, mr.czauderna@gmail.com,
Marounek, M.
Prague-Uhříněves The Institute of Animal Science Prague Czech Republic,
Duskova, D.
Prague-Uhříněves The Institute of Animal Science Prague Czech Republic,
Kowalczyk, J.
Polish Academy of Sciences The Kielanowski Institute of Animal Physiology and Nutrition 05-110 Jabłonna Poland,
Konda, R. K.
Hindu College of Pharmacy Department of Pharmaceutical Chemistry Amaravathi Road Guntur Andhrapradesh 522002 India, victory2ravi@yahoo.co.in,
Chandu, B. R.
Donbosco College of Pharmacy Department of Pharmaceutical Sciences Pulladigunta Guntur 522201 India,
Challa, B. R.
Nirmala College of Pharmacy Department of Pharmaceutical Analysis Madras Road Kadapa Andhrapradesh 516002 India, baluchalla_99@yahoo.com,
Chandrasekhar, K. B.
Jawaharlal Nehru Technological University Department of Chemistry Anantapur 515002 India,
Shen, Y.
Jiangsu University School of Pharmacy Zhenjiang China,
Yang, H.
Jiangsu University School of Pharmacy Zhenjiang China,
Xia, G.
Jiangsu University School of Pharmacy Zhenjiang China,
Wang, J.
Jiangsu University School of Pharmacy Zhenjiang China,
Cai, B.
Nanjing University of Chinese Medicine Jiangsu Key Laboratory of Chinese Medicine Processing, SATCM Key Laboratory of Standardization for Chinese Medicine Processing Nanjing China,
Jia, X.
Jiangsu University School of Pharmacy Zhenjiang China, jxiaobin2005@hotmail.com,
Nurulain, S. M.
United Arab Emirates University Department of Pharmacology and Pharmacotherapy Al Ain United Arab Emirates,
Kalász, H.
Semmelweis University Department of Pharmacology and Pharmacotherapy Budapest Hungary, huba.kalasz@gmail.com,
Szegi, P.
Semmelweis University Department of Pharmacodynamics Budapest Hungary,
Kuca, K.
University Hospital Biomedical Research Center Hradec Kralove Czech Republic,
Adem, A.
United Arab Emirates University Department of Pharmacology and Pharmacotherapy Al Ain United Arab Emirates,
Hasan, M. Y. B.
United Arab Emirates University Department of Pharmacology and Pharmacotherapy Al Ain United Arab Emirates,
Hashemi, F.
Semmelweis University Department of Pharmacodynamics Budapest Hungary,
Tekes, K.
Semmelweis University Department of Pharmacodynamics Budapest Hungary,
Sangoi, M. S.
Federal University of Rio Grande do Sul Faculty of Pharmacy 90610-000 Porto Alegre-RS Brazil, maxsilvasangoi@yahoo.com.br,
Wrasse-Sangoi, M.
Federal University of Santa Maria Department of Industrial Pharmacy 97105-900 Santa Maria-RS Brazil,
Hurtado, F. K.
Federal University of Rio Grande do Sul Faculty of Pharmacy 90610-000 Porto Alegre-RS Brazil,
Rolim, C. M. B.
Federal University of Santa Maria Department of Industrial Pharmacy 97105-900 Santa Maria-RS Brazil,
Abstrakty : A sensitive, fast, and stability-indicating micellar electrokinetic chromatographic (MEKC) method was developed and validated for the analysis of aliskiren (ALI) in tablets using nimesulide as internal standard (IS). Optimal conditions for the separation of ALI and its degradation products were investigated. The method employed 60 mM Tris buffer and 50 mM anionic detergent sodium dodecyl sulfate (SDS) solution at pH 9.8. MEKC method was performed on a fused-silica capillary (50 μm id; effective length, 40 cm). The capillary temperature was maintained at 35°C, and the applied voltage was 30 kV, with detection by photodiode array (PDA) detector set at 204 nm. The method was validated in accordance with the International Conference on Harmonisation (ICH) requirements. The stability-indicating capability of the method was established by enforced degradation studies combined with peak purity assessment using PDA detection. The method was linear over the concentration range of 5–150 μg mL-1(r2 = 0.9996) of ALI. Intraday and interday precision and accuracy evaluated by relative standard deviation, respectively, were lower than 2%. The limit of detection was 1.31 μg mL-1. The method proved to be robust by a one-variable-at-a-time evaluation. The proposed MEKC method was successfully applied for the quantitative analysis of ALI in tablets to support the quality control.

Słowa kluczowe : aliskiren, MEKC, stability-indicating method, stress degradation studies, validation,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2013
Numer : Vol. 25, no. 4
Strony : 711 – 724
Bibliografia : 1 R.E. Schmieder, Hipertens. Riesgo Vasc., 27(6), 245 (2010)
2 S. Lam and M. Choy, Cardiol. Rev., 15(6), 316 (2007)
3 S. Vaidyanathan, V. Jarugula, H.A. Dieterich, D. Howard, and W.P. Dole, Clin. Pharmacokinet., 47(8), 515 (2008)
4 L. Suntornsuk, Anal. Bioanal. Chem., 398, 29 (2010)
5 U. Holzgrabe, D. Brinz, S. Kopec, C. Weber, and Y. Bitar, Electrophoresis, 27, 2283 (2006)
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7 M.S. Sangoi, L.M. Silva, F.B. D’Avila, and S.L. Dalmora, J. Chromatogr. Sci., 48, 641 (2010)
8 S. El Deeb, M.A. Iriban, and R. Gust, Electrophoresis, 32, 166 (2011)
9 M. Wrasse-Sangoi, M.S. Sangoi, P.R. Oliveira, L.T. Secretti, and C.M.B. Rolim, J. Chromatogr. Sci., 49, 170 (2011)
10 M. Wrasse-Sangoi, L.T. Secretti, I.F. Diefenbach, C.M.B. Rolim, and M.S. Sangoi, Quim. Nova, 33(6), 1330 (2010)
11 M.S. Sangoi, M. Wrasse-Sangoi, P.R. Oliveira, V. Todeschini, and C.M.B. Rolim, J. Liq. Chromatogr. Relat. Technol., 34, 1976 (2011)
12 M.S. Sangoi, M. Wrasse-Sangoi, P.R. Oliveira, C.M.B. Rolim, and M. Steppe, J. Sep. Sci., 34, 1859 (2011)
13 International Conference on Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human Use, Stability testing of New Drug Substances and Products, Q1A(R2), Geneva, August, 2003
14 International Conference on Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human Use, Validation of Analytical Procedures: Text and Methodology, Q2(R1), Geneva, November, 2005
15 R. Injac, M. Boskovic, N. Kocevar, and T. Vovk, Anal. Chim. Acta, 620, 150 (2008)
16 B. Dejaegher and Y.V. Heyden, J. Chromatogr. A, 1158, 138 (2007)
17 United States Pharmacopeial Convention, The United States Pharmacopeia, 32th ed., Rockville, MD, 2009
18 K.L. See, A.A. Elbashir, B. Saad, A.S.M. Ali, and H.Y. Aboul-Enein, Biomed. Chromatogr., 23, 1283 (2009)
19 K.M. Alsante, A. Ando, R. Brown, J. Ensing, T.D. Hatajik, W. Kong, and Y. Tsuda, Adv. Drug Deliv. Rev., 59, 29 (2007)
20 S. Görög, J. Pharm. Biomed. Anal., 48, 247 (2008)
21 E. Rozet, A. Ceccato, C. Hubert, E. Ziemons, R. Oprean, S. Rudaz, B. Boulanger, and P. Hubert, J. Chromatogr. A, 1158, 111 (2007)
22 G.A. Shabir, J. Chromatogr. A, 987, 57 (2003)
DOI :
Cytuj : Patil, A. N. ,Nirmal, S. A. ,Chavan, A. K. ,Zhou, L. ,Xu, J. ,Luan, L. ,Ma, J. ,Gong, Y. ,Qin, D. ,Pan, C. ,Jinlong, M. ,Guobin, J. ,Bo, J. ,Hua, J. ,Montejo, C. ,Civera, C. ,Barcia, E. ,Negro, S. ,Fernández-Carballido, A. ,Czauderna, M. ,Marounek, M. ,Duskova, D. ,Kowalczyk, J. ,Konda, R. K. ,Chandu, B. R. ,Challa, B. R. ,Chandrasekhar, K. B. ,Shen, Y. ,Yang, H. ,Xia, G. ,Wang, J. ,Cai, B. ,Jia, X. ,Nurulain, S. M. ,Kalász, H. ,Szegi, P. ,Kuca, K. ,Adem, A. ,Hasan, M. Y. B. ,Hashemi, F. ,Tekes, K. ,Sangoi, M. S. ,Wrasse-Sangoi, M. ,Hurtado, F. K. ,Rolim, C. M. B. , Stress degradation studies on aliskiren and the development of a sensitive stability-indicating MEKC method. Acta Chromatographica Vol. 25, no. 4/2013
[Top]

Improved method for determination of the fungicide dimethomorph in vegetables

Czasopismo : Acta Chromatographica
Tytuł artykułu : Improved method for determination of the fungicide dimethomorph in vegetables

Autorzy :
Patil, A. N.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India,
Nirmal, S. A.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India, nirmalsunil@rediffmail.com,
Chavan, A. K.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India,
Zhou, L.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Xu, J.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Luan, L.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Ma, J.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Gong, Y.
Ministry of Agriculture Institute for the Control of Agrochemicals Beijing 100026 China,
Qin, D.
Ministry of Agriculture Institute for the Control of Agrochemicals Beijing 100026 China,
Pan, C.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Jinlong, M.
Zhejiang University Department of Chemical and Biological Engineering Hangzhou 310027 China,
Guobin, J.
Dalian Nationalities University Environment and Resources College Dalian 11660 China,
Bo, J.
Dalian Nationalities University Life Science College Dalian 116600 China,
Hua, J.
Zhejiang University Department of Chemical and Biological Engineering Hangzhou 310027 China,
Montejo, C.
Universidad CEU San Pablo Departamento de Ciencias Farmacéuticas y de la Alimentación, Área de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28668 Madrid Spain, montejo@ceu.es,
Civera, C.
Universidad Complutense de Madrid Departamento de Química Física II, Facultad de Farmacia 28040 Madrid Spain,
Barcia, E.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Negro, S.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Fernández-Carballido, A.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Czauderna, M.
Polish Academy of Sciences The Kielanowski Institute of Animal Physiology and Nutrition 05-110 Jabłonna Poland, mr.czauderna@gmail.com,
Marounek, M.
Prague-Uhříněves The Institute of Animal Science Prague Czech Republic,
Duskova, D.
Prague-Uhříněves The Institute of Animal Science Prague Czech Republic,
Kowalczyk, J.
Polish Academy of Sciences The Kielanowski Institute of Animal Physiology and Nutrition 05-110 Jabłonna Poland,
Konda, R. K.
Hindu College of Pharmacy Department of Pharmaceutical Chemistry Amaravathi Road Guntur Andhrapradesh 522002 India, victory2ravi@yahoo.co.in,
Chandu, B. R.
Donbosco College of Pharmacy Department of Pharmaceutical Sciences Pulladigunta Guntur 522201 India,
Challa, B. R.
Nirmala College of Pharmacy Department of Pharmaceutical Analysis Madras Road Kadapa Andhrapradesh 516002 India, baluchalla_99@yahoo.com,
Chandrasekhar, K. B.
Jawaharlal Nehru Technological University Department of Chemistry Anantapur 515002 India,
Shen, Y.
Jiangsu University School of Pharmacy Zhenjiang China,
Yang, H.
Jiangsu University School of Pharmacy Zhenjiang China,
Xia, G.
Jiangsu University School of Pharmacy Zhenjiang China,
Wang, J.
Jiangsu University School of Pharmacy Zhenjiang China,
Cai, B.
Nanjing University of Chinese Medicine Jiangsu Key Laboratory of Chinese Medicine Processing, SATCM Key Laboratory of Standardization for Chinese Medicine Processing Nanjing China,
Jia, X.
Jiangsu University School of Pharmacy Zhenjiang China, jxiaobin2005@hotmail.com,
Nurulain, S. M.
United Arab Emirates University Department of Pharmacology and Pharmacotherapy Al Ain United Arab Emirates,
Kalász, H.
Semmelweis University Department of Pharmacology and Pharmacotherapy Budapest Hungary, huba.kalasz@gmail.com,
Szegi, P.
Semmelweis University Department of Pharmacodynamics Budapest Hungary,
Kuca, K.
University Hospital Biomedical Research Center Hradec Kralove Czech Republic,
Adem, A.
United Arab Emirates University Department of Pharmacology and Pharmacotherapy Al Ain United Arab Emirates,
Hasan, M. Y. B.
United Arab Emirates University Department of Pharmacology and Pharmacotherapy Al Ain United Arab Emirates,
Hashemi, F.
Semmelweis University Department of Pharmacodynamics Budapest Hungary,
Tekes, K.
Semmelweis University Department of Pharmacodynamics Budapest Hungary,
Sangoi, M. S.
Federal University of Rio Grande do Sul Faculty of Pharmacy 90610-000 Porto Alegre-RS Brazil, maxsilvasangoi@yahoo.com.br,
Wrasse-Sangoi, M.
Federal University of Santa Maria Department of Industrial Pharmacy 97105-900 Santa Maria-RS Brazil,
Hurtado, F. K.
Federal University of Rio Grande do Sul Faculty of Pharmacy 90610-000 Porto Alegre-RS Brazil,
Rolim, C. M. B.
Federal University of Santa Maria Department of Industrial Pharmacy 97105-900 Santa Maria-RS Brazil,
Walorczyk, S.
Institute of Plant Protection — National Research Institute Department of Pesticide Residue Research Władysława Węgorka 20 60-318 Poznań Poland, s_walorczyk@tlen.pl,
Abstrakty : An improved analytical method for determining the fungicide dimethomorph in vegetables is described. The method involved single extraction with dichloromethane followed by gas chromatography-tandem mass spectrometry (GC-MS/MS) determination. The average recovery rates from vegetable samples spiked with dimethomorph at 10 and 100 μg kg-1(n = 5) ranged between 81 and 96% and with associated relative standard deviations ≤9%. The limits of detection (LOD) and limits of quantification (LOQ) were below 10 μg kg-1for the three studied matrices, i.e., tomato, cucumber, and onion. Successful application of the method to the analysis of samples with incurred dimethomorph residues has been demonstrated.

Słowa kluczowe : pesticide residue analysis, extraction methods, vegetables, gas chromatography, mass spectrometry,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2013
Numer : Vol. 25, no. 4
Strony : 725 – 733
Bibliografia : 1 C.D.S. Tomlin (ed.) The Pesticide Manual, British Crop Protection Council, Farnham, 1997, pp. 416–418
2 Ö. Tokuşoğlu, M.K. Ünal, and Z. Yıldırım, Acta Chromatogr., 13, 196 (2003)
3 M. Cabizza, F. Dedola, and M. Satta, J. Environ. Sci. Health B, 47, 379 (2012)
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5 L.V. Podhorniak, F.J. Schenck, A. Krynitsky, and D.J. Griffith, J. AOAC Int., 85, 1237 (2004)
6 S. Walorczyk and B. Gnusowski, J. Chromatogr. A, 1128, 236 (2006)
7 S.J. Lehotay, K. Ae Son, H. Kwon, U. Koesukwiwat, W. Fu, K. Mastovska, E. Hoh, and N. Leepipatpiboon, J. Chromatogr. A, 1217, 2548 (2010)
8 J.L. Martinez Vidal, F.J. Arrebola, A. Garrido Frenich, J.M. Fernandez, and M. Mateu-Sanchez, Chromatographia, 59, 321 (2004)
9 A. M. Filho, F. N. dos Santos, and P.A. de Paula Pereira, Talanta, 81, 346 (2010)
10 H.V. Botitsi, S.D. Garbis, A. Economou, and D.F. Tsipi, Mass Spectrom. Rev., 30, 907 (2011)
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14 Method validation and quality control procedures for pesticide residues analysis in food and feed. Document N° SANCO/12495/2011 http://ec.europa.eu/food/plant/protection/pesticides/docs/qualcontrol_en.pdf
15 S. Walorczyk, J. Chromatogr. A, 1165, 200 (2007)
16 Pesticide EU-MRLs. Regulation (EC) No 396/2005 http://ec.europa.eu/sanco_pesticides/public/index.cfm
DOI :
Cytuj : Patil, A. N. ,Nirmal, S. A. ,Chavan, A. K. ,Zhou, L. ,Xu, J. ,Luan, L. ,Ma, J. ,Gong, Y. ,Qin, D. ,Pan, C. ,Jinlong, M. ,Guobin, J. ,Bo, J. ,Hua, J. ,Montejo, C. ,Civera, C. ,Barcia, E. ,Negro, S. ,Fernández-Carballido, A. ,Czauderna, M. ,Marounek, M. ,Duskova, D. ,Kowalczyk, J. ,Konda, R. K. ,Chandu, B. R. ,Challa, B. R. ,Chandrasekhar, K. B. ,Shen, Y. ,Yang, H. ,Xia, G. ,Wang, J. ,Cai, B. ,Jia, X. ,Nurulain, S. M. ,Kalász, H. ,Szegi, P. ,Kuca, K. ,Adem, A. ,Hasan, M. Y. B. ,Hashemi, F. ,Tekes, K. ,Sangoi, M. S. ,Wrasse-Sangoi, M. ,Hurtado, F. K. ,Rolim, C. M. B. ,Walorczyk, S. , Improved method for determination of the fungicide dimethomorph in vegetables. Acta Chromatographica Vol. 25, no. 4/2013
[Top]

Development and validation of a RP-HPLC method for simultaneous determination of salicin and eight flavonoids in leaves of Salix Matsudana Koidz

Czasopismo : Acta Chromatographica
Tytuł artykułu : Development and validation of a RP-HPLC method for simultaneous determination of salicin and eight flavonoids in leaves of Salix Matsudana Koidz

Autorzy :
Patil, A. N.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India,
Nirmal, S. A.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India, nirmalsunil@rediffmail.com,
Chavan, A. K.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India,
Zhou, L.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Xu, J.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Luan, L.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Ma, J.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Gong, Y.
Ministry of Agriculture Institute for the Control of Agrochemicals Beijing 100026 China,
Qin, D.
Ministry of Agriculture Institute for the Control of Agrochemicals Beijing 100026 China,
Pan, C.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Jinlong, M.
Zhejiang University Department of Chemical and Biological Engineering Hangzhou 310027 China,
Guobin, J.
Dalian Nationalities University Environment and Resources College Dalian 11660 China,
Bo, J.
Dalian Nationalities University Life Science College Dalian 116600 China,
Hua, J.
Zhejiang University Department of Chemical and Biological Engineering Hangzhou 310027 China,
Montejo, C.
Universidad CEU San Pablo Departamento de Ciencias Farmacéuticas y de la Alimentación, Área de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28668 Madrid Spain, montejo@ceu.es,
Civera, C.
Universidad Complutense de Madrid Departamento de Química Física II, Facultad de Farmacia 28040 Madrid Spain,
Barcia, E.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Negro, S.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Fernández-Carballido, A.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Czauderna, M.
Polish Academy of Sciences The Kielanowski Institute of Animal Physiology and Nutrition 05-110 Jabłonna Poland, mr.czauderna@gmail.com,
Marounek, M.
Prague-Uhříněves The Institute of Animal Science Prague Czech Republic,
Duskova, D.
Prague-Uhříněves The Institute of Animal Science Prague Czech Republic,
Kowalczyk, J.
Polish Academy of Sciences The Kielanowski Institute of Animal Physiology and Nutrition 05-110 Jabłonna Poland,
Konda, R. K.
Hindu College of Pharmacy Department of Pharmaceutical Chemistry Amaravathi Road Guntur Andhrapradesh 522002 India, victory2ravi@yahoo.co.in,
Chandu, B. R.
Donbosco College of Pharmacy Department of Pharmaceutical Sciences Pulladigunta Guntur 522201 India,
Challa, B. R.
Nirmala College of Pharmacy Department of Pharmaceutical Analysis Madras Road Kadapa Andhrapradesh 516002 India, baluchalla_99@yahoo.com,
Chandrasekhar, K. B.
Jawaharlal Nehru Technological University Department of Chemistry Anantapur 515002 India,
Shen, Y.
Jiangsu University School of Pharmacy Zhenjiang China,
Yang, H.
Jiangsu University School of Pharmacy Zhenjiang China,
Xia, G.
Jiangsu University School of Pharmacy Zhenjiang China,
Wang, J.
Jiangsu University School of Pharmacy Zhenjiang China,
Cai, B.
Nanjing University of Chinese Medicine Jiangsu Key Laboratory of Chinese Medicine Processing, SATCM Key Laboratory of Standardization for Chinese Medicine Processing Nanjing China,
Jia, X.
Jiangsu University School of Pharmacy Zhenjiang China, jxiaobin2005@hotmail.com,
Nurulain, S. M.
United Arab Emirates University Department of Pharmacology and Pharmacotherapy Al Ain United Arab Emirates,
Kalász, H.
Semmelweis University Department of Pharmacology and Pharmacotherapy Budapest Hungary, huba.kalasz@gmail.com,
Szegi, P.
Semmelweis University Department of Pharmacodynamics Budapest Hungary,
Kuca, K.
University Hospital Biomedical Research Center Hradec Kralove Czech Republic,
Adem, A.
United Arab Emirates University Department of Pharmacology and Pharmacotherapy Al Ain United Arab Emirates,
Hasan, M. Y. B.
United Arab Emirates University Department of Pharmacology and Pharmacotherapy Al Ain United Arab Emirates,
Hashemi, F.
Semmelweis University Department of Pharmacodynamics Budapest Hungary,
Tekes, K.
Semmelweis University Department of Pharmacodynamics Budapest Hungary,
Sangoi, M. S.
Federal University of Rio Grande do Sul Faculty of Pharmacy 90610-000 Porto Alegre-RS Brazil, maxsilvasangoi@yahoo.com.br,
Wrasse-Sangoi, M.
Federal University of Santa Maria Department of Industrial Pharmacy 97105-900 Santa Maria-RS Brazil,
Hurtado, F. K.
Federal University of Rio Grande do Sul Faculty of Pharmacy 90610-000 Porto Alegre-RS Brazil,
Rolim, C. M. B.
Federal University of Santa Maria Department of Industrial Pharmacy 97105-900 Santa Maria-RS Brazil,
Walorczyk, S.
Institute of Plant Protection — National Research Institute Department of Pesticide Residue Research Władysława Węgorka 20 60-318 Poznań Poland, s_walorczyk@tlen.pl,
Li, W.
Jilin Agricultural University College of Chinese Medicinal Material Changchun 130118 China,
Shi, L. L.
Jilin Agricultural University College of Chinese Medicinal Material Changchun 130118 China,
Han, L. Q.
Jilin Agricultural University College of Chinese Medicinal Material Changchun 130118 China,
Zhang, J.
Jilin Agricultural University College of Chinese Medicinal Material Changchun 130118 China, zhjing0701@163.com,
Abstrakty : A reversed-phase high-performance liquid chromatographic method was developed for the first time to simultaneously determine salicin and eight flavonoids in leaves of Salix matsudana, that is salicin, luteolin-7-O-glucoside, myricetin, apigenin-3′-oxyethyl-7-O-glucoside, rutin, quercetin, luteolin, kaempferol and apigenin. The separation of these compounds was achieved on a reversed phase C18 column (250 × 4.6 mm, 5 μm), with linear gradient of methanol in 0.2% phosphoric acid solution with a flow rate of 1.0 mL/min with UV detection at 246 nm. The calibration curves for the determination of all analytes showed good linearity over the investigated ranges (r > 0.999). The % relative standard deviation (% RSD) values were less than 0.34%, and the recoveries were between 95.79% and 99.94%. The values of luteolin-7-O-glucoside, salicin, myricetin, apigenin-3′-oxyethyl-7-O-glucoside, rutin, quercetin, luteolin, kaempferol, and apigenin were 1.0 μg g-1, 20.0 μg g-1, 32.9 μg g-1, 2.0 μg g-1, 29.5 μg g-1, 6.0 μg g-1, 1.0 μg g-1, 3.5 μg g-1, and apigenin was not found in the sample. This developed method can be used for evaluating the quality of different plant materials.

Słowa kluczowe : high performance liquid chromatography (HPLC), quality control, salicin and flavonoid, Salix matsudana,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2013
Numer : Vol. 25, no. 4
Strony : 735 – 743
Bibliografia : 1 J. Wang, S. Zheng, T. Shen, and X. Shen, Chin. Chem. Lett., 5, 432–435 (2002).
2 C. Xu, Y. Zheng, X. Yang, X. Li, and Q. Chen, J. Asian Nat. Prod. Res., 9, 415–419 (2007)
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4 G.A. Boeckler, J. Gershenzon, and S.B. Unsicker, Phytochemistry, 13, 1497–1509 (2011)
5 C.E. Caristi, V. Bellocco, G. Panzera, R. Toscano, and U. Leuzzi, J. Agric. Food Chem., 51, 3528–3534 (2003)
6 P. Dugo, M.L. Presti, M. Ohman, A. Fazio, G. Dugo, and L. Mondello, J. Sep. Sci., 28, 1149–1156 (2005)
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17 Y. Zhang, B. Bao, B. Lu, Y. Ren, X. Tie, and Y. Zhang, J. Chromatogr. A, 1065, 177–185 (2005)
18 Y.N. Zheng, J. Zhang, L.K. Han, K. Sekiya, Y. Kimura, and H. Okuda, Yakugaku Zasshi, 125, 1005–1008 (2005)
19 Z.H. Shi, J.T. He, T.T. Yao, and W.B. Chang, J. Liq. Chromatogr. R.T., 27, 465–479 (2005)
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DOI :
Cytuj : Patil, A. N. ,Nirmal, S. A. ,Chavan, A. K. ,Zhou, L. ,Xu, J. ,Luan, L. ,Ma, J. ,Gong, Y. ,Qin, D. ,Pan, C. ,Jinlong, M. ,Guobin, J. ,Bo, J. ,Hua, J. ,Montejo, C. ,Civera, C. ,Barcia, E. ,Negro, S. ,Fernández-Carballido, A. ,Czauderna, M. ,Marounek, M. ,Duskova, D. ,Kowalczyk, J. ,Konda, R. K. ,Chandu, B. R. ,Challa, B. R. ,Chandrasekhar, K. B. ,Shen, Y. ,Yang, H. ,Xia, G. ,Wang, J. ,Cai, B. ,Jia, X. ,Nurulain, S. M. ,Kalász, H. ,Szegi, P. ,Kuca, K. ,Adem, A. ,Hasan, M. Y. B. ,Hashemi, F. ,Tekes, K. ,Sangoi, M. S. ,Wrasse-Sangoi, M. ,Hurtado, F. K. ,Rolim, C. M. B. ,Walorczyk, S. ,Li, W. ,Shi, L. L. ,Han, L. Q. ,Zhang, J. , Development and validation of a RP-HPLC method for simultaneous determination of salicin and eight flavonoids in leaves of Salix Matsudana Koidz. Acta Chromatographica Vol. 25, no. 4/2013
[Top]

LC-MS/MS method for the quantification of withaferin-A in plant extracts of Withania spp.

Czasopismo : Acta Chromatographica
Tytuł artykułu : LC-MS/MS method for the quantification of withaferin-A in plant extracts of Withania spp.

Autorzy :
Patil, A. N.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India,
Nirmal, S. A.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India, nirmalsunil@rediffmail.com,
Chavan, A. K.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India,
Zhou, L.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Xu, J.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Luan, L.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Ma, J.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Gong, Y.
Ministry of Agriculture Institute for the Control of Agrochemicals Beijing 100026 China,
Qin, D.
Ministry of Agriculture Institute for the Control of Agrochemicals Beijing 100026 China,
Pan, C.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Jinlong, M.
Zhejiang University Department of Chemical and Biological Engineering Hangzhou 310027 China,
Guobin, J.
Dalian Nationalities University Environment and Resources College Dalian 11660 China,
Bo, J.
Dalian Nationalities University Life Science College Dalian 116600 China,
Hua, J.
Zhejiang University Department of Chemical and Biological Engineering Hangzhou 310027 China,
Montejo, C.
Universidad CEU San Pablo Departamento de Ciencias Farmacéuticas y de la Alimentación, Área de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28668 Madrid Spain, montejo@ceu.es,
Civera, C.
Universidad Complutense de Madrid Departamento de Química Física II, Facultad de Farmacia 28040 Madrid Spain,
Barcia, E.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Negro, S.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Fernández-Carballido, A.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Czauderna, M.
Polish Academy of Sciences The Kielanowski Institute of Animal Physiology and Nutrition 05-110 Jabłonna Poland, mr.czauderna@gmail.com,
Marounek, M.
Prague-Uhříněves The Institute of Animal Science Prague Czech Republic,
Duskova, D.
Prague-Uhříněves The Institute of Animal Science Prague Czech Republic,
Kowalczyk, J.
Polish Academy of Sciences The Kielanowski Institute of Animal Physiology and Nutrition 05-110 Jabłonna Poland,
Konda, R. K.
Hindu College of Pharmacy Department of Pharmaceutical Chemistry Amaravathi Road Guntur Andhrapradesh 522002 India, victory2ravi@yahoo.co.in,
Chandu, B. R.
Donbosco College of Pharmacy Department of Pharmaceutical Sciences Pulladigunta Guntur 522201 India,
Challa, B. R.
Nirmala College of Pharmacy Department of Pharmaceutical Analysis Madras Road Kadapa Andhrapradesh 516002 India, baluchalla_99@yahoo.com,
Chandrasekhar, K. B.
Jawaharlal Nehru Technological University Department of Chemistry Anantapur 515002 India,
Shen, Y.
Jiangsu University School of Pharmacy Zhenjiang China,
Yang, H.
Jiangsu University School of Pharmacy Zhenjiang China,
Xia, G.
Jiangsu University School of Pharmacy Zhenjiang China,
Wang, J.
Jiangsu University School of Pharmacy Zhenjiang China,
Cai, B.
Nanjing University of Chinese Medicine Jiangsu Key Laboratory of Chinese Medicine Processing, SATCM Key Laboratory of Standardization for Chinese Medicine Processing Nanjing China,
Jia, X.
Jiangsu University School of Pharmacy Zhenjiang China, jxiaobin2005@hotmail.com,
Nurulain, S. M.
United Arab Emirates University Department of Pharmacology and Pharmacotherapy Al Ain United Arab Emirates,
Kalász, H.
Semmelweis University Department of Pharmacology and Pharmacotherapy Budapest Hungary, huba.kalasz@gmail.com,
Szegi, P.
Semmelweis University Department of Pharmacodynamics Budapest Hungary,
Kuca, K.
University Hospital Biomedical Research Center Hradec Kralove Czech Republic,
Adem, A.
United Arab Emirates University Department of Pharmacology and Pharmacotherapy Al Ain United Arab Emirates,
Hasan, M. Y. B.
United Arab Emirates University Department of Pharmacology and Pharmacotherapy Al Ain United Arab Emirates,
Hashemi, F.
Semmelweis University Department of Pharmacodynamics Budapest Hungary,
Tekes, K.
Semmelweis University Department of Pharmacodynamics Budapest Hungary,
Sangoi, M. S.
Federal University of Rio Grande do Sul Faculty of Pharmacy 90610-000 Porto Alegre-RS Brazil, maxsilvasangoi@yahoo.com.br,
Wrasse-Sangoi, M.
Federal University of Santa Maria Department of Industrial Pharmacy 97105-900 Santa Maria-RS Brazil,
Hurtado, F. K.
Federal University of Rio Grande do Sul Faculty of Pharmacy 90610-000 Porto Alegre-RS Brazil,
Rolim, C. M. B.
Federal University of Santa Maria Department of Industrial Pharmacy 97105-900 Santa Maria-RS Brazil,
Walorczyk, S.
Institute of Plant Protection — National Research Institute Department of Pesticide Residue Research Władysława Węgorka 20 60-318 Poznań Poland, s_walorczyk@tlen.pl,
Li, W.
Jilin Agricultural University College of Chinese Medicinal Material Changchun 130118 China,
Shi, L. L.
Jilin Agricultural University College of Chinese Medicinal Material Changchun 130118 China,
Han, L. Q.
Jilin Agricultural University College of Chinese Medicinal Material Changchun 130118 China,
Zhang, J.
Jilin Agricultural University College of Chinese Medicinal Material Changchun 130118 China, zhjing0701@163.com,
Mirjalili, M. H.
Shahid Beheshti University Medicinal Plants and Drugs Research Institute G.C., Evin Tehran Iran,
Navarro, A.
National Autonomous University of Mexico Department of Food and Biotechnology, Faculty of Chemistry Ciudad de México 04510 Mexico,
Hernández, L.
Universidad Autónoma Metropolitana-Xochimilco Departamento de Sistemas Biológicos Calz. del Hueso 1100, Col. Villa Quietud 04960 México, D. F. México,
Jáuregui, O.
Universitat de Barcelona Scientific & Technological Centers (CCiTUB) c/Baldiri i Reixach 10-12 08028 Barcelona Spain,
Bonfill, M.
Universitat de Barcelona Laboratori de Fisiologia Vegetal, Facultat de Farmàcia Avda. Joan XXIII s/n 08028 Barcelona Spain, mbonfill@ub.edu,
Abstrakty : The quantification of the main withanolide, withaferin-A, in plant extracts of Withania somnifera and Withania coagulans using high-performance liquid chromatography (HPLC)-mass spectrometry (ESI-MS/MS) is described. HPLC was used in combination with a triple quadrupole mass spectrometer in multiple reaction monitoring (MRM) mode. Additionally, mass exact determination of withaferin-A was achieved using a hybrid high-resolution mass spectrometer (Q-TOF). A combination of acetonitrile and water as the mobile phase using gradient elution was found to be successful in separating the compound of interest from the other main components. The limit of detection (LOD) in the plants was 6 ng/g and the total analysis time was 10 min.

Słowa kluczowe : Withania somnifera, Withania coagulans, withaferin-A, liquid chromatography, mass spectrometry, exact mass measurement,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2013
Numer : Vol. 25, no. 4
Strony : 745 – 754
Bibliografia : 1 G.L. Gupta and A.C. Rana, Pharmacogn. Rev., 1, 129 (2007)
2 P. Bargagna-Mohan, A. Hamza, Y-E. Kim, Y.K. Ho, N. Mor-Vaknin, N. Wendschlag, J. Liu, R.M. Evans, D.M. Markovitz, C-G. Zhan, K.B. Kim, and R. Mohan, Chemistry & Biology, 14, 623 (2007)
3 R.S. Sangwan, N.D. Chaurasiya, L.N. Misra, P. Lal, G.C. Uniyal, R. Sharma, N.S. Sangwan, K.A. Suri, G.N. Qazi, and R. Tuli, Current Sci., 86, 461 (2004)
4 M. Ganzera, M.I. Choudhary, and I.A. Khan, Fitoterapia, 74, 68 (2003)
5 N.D. Chaurasiya, G.C. Uniyal, P. Lal, L. Misra, N.S. Sangwan, R. Tuli, and R.S. Sangwan, Phytochem. Anal., 19, 148 (2008)
6 A.B. Ray and M. Gupta, Prog. Chem. Org. Nat. Prod., 63, 1 (1994)
7 L. Dinan, J. Harmatha, and R. Lafont, J. Chromatogr. A, 935, 105 (2001)
8 M.H. Mirjalili, S.M.F. Tabatabaei, H. Alizadeh, A. Ghassempour, and F. Mirzajani, Nat. Prod. Commun., 4, 337 (2009)
9 R.K. Khajuria, K.A. Suri, R.K. Gupta, N.K. Satti, M. Amina, O.P. Suri, and G.N. Qazi, J. Sep. Sci., 27, 541–546 (2004)
10 B. Kaufmann, S. Souverain, S. Cherkaoui, P. Christen, and J.L. Veuthey, Chromatographia, 56, 137 (2002)
11 M.H. Mirjalili, S.M. Fakhr-Tabatabaei, M. Bonfill, H. Alizadeh, R.M. Cusido, A. Ghassempour, and J. Palazon, Engineering in Life Sci., 9, 197 (2009)
12 V. Sharma, A.P. Gupta, P. Bhandari, R.C. Gupta, and B. Singh, Chromatographia, 66, 801 (2007)
13 M. Bandyopadhyay, S. Jha, and D. Tapfer, Plant Cell Rep., 26, 599 (2007)
14 M. Sharada, A. Ahuja, K.A. Suri, S.P. Vij, R.K. Khajuria, V. Vermaand, and A. Kumar, Biol. Plantarum, 51, 161 (2007)
DOI :
Cytuj : Patil, A. N. ,Nirmal, S. A. ,Chavan, A. K. ,Zhou, L. ,Xu, J. ,Luan, L. ,Ma, J. ,Gong, Y. ,Qin, D. ,Pan, C. ,Jinlong, M. ,Guobin, J. ,Bo, J. ,Hua, J. ,Montejo, C. ,Civera, C. ,Barcia, E. ,Negro, S. ,Fernández-Carballido, A. ,Czauderna, M. ,Marounek, M. ,Duskova, D. ,Kowalczyk, J. ,Konda, R. K. ,Chandu, B. R. ,Challa, B. R. ,Chandrasekhar, K. B. ,Shen, Y. ,Yang, H. ,Xia, G. ,Wang, J. ,Cai, B. ,Jia, X. ,Nurulain, S. M. ,Kalász, H. ,Szegi, P. ,Kuca, K. ,Adem, A. ,Hasan, M. Y. B. ,Hashemi, F. ,Tekes, K. ,Sangoi, M. S. ,Wrasse-Sangoi, M. ,Hurtado, F. K. ,Rolim, C. M. B. ,Walorczyk, S. ,Li, W. ,Shi, L. L. ,Han, L. Q. ,Zhang, J. ,Mirjalili, M. H. ,Navarro, A. ,Hernández, L. ,Jáuregui, O. ,Bonfill, M. , LC-MS/MS method for the quantification of withaferin-A in plant extracts of Withania spp.. Acta Chromatographica Vol. 25, no. 4/2013
[Top]

Simultaneous determination of lycorine and galanthamine in Galanthus woronowii by HPLC-DAD

Czasopismo : Acta Chromatographica
Tytuł artykułu : Simultaneous determination of lycorine and galanthamine in Galanthus woronowii by HPLC-DAD

Autorzy :
Patil, A. N.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India,
Nirmal, S. A.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India, nirmalsunil@rediffmail.com,
Chavan, A. K.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India,
Zhou, L.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Xu, J.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Luan, L.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Ma, J.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Gong, Y.
Ministry of Agriculture Institute for the Control of Agrochemicals Beijing 100026 China,
Qin, D.
Ministry of Agriculture Institute for the Control of Agrochemicals Beijing 100026 China,
Pan, C.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Jinlong, M.
Zhejiang University Department of Chemical and Biological Engineering Hangzhou 310027 China,
Guobin, J.
Dalian Nationalities University Environment and Resources College Dalian 11660 China,
Bo, J.
Dalian Nationalities University Life Science College Dalian 116600 China,
Hua, J.
Zhejiang University Department of Chemical and Biological Engineering Hangzhou 310027 China,
Montejo, C.
Universidad CEU San Pablo Departamento de Ciencias Farmacéuticas y de la Alimentación, Área de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28668 Madrid Spain, montejo@ceu.es,
Civera, C.
Universidad Complutense de Madrid Departamento de Química Física II, Facultad de Farmacia 28040 Madrid Spain,
Barcia, E.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Negro, S.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Fernández-Carballido, A.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Czauderna, M.
Polish Academy of Sciences The Kielanowski Institute of Animal Physiology and Nutrition 05-110 Jabłonna Poland, mr.czauderna@gmail.com,
Marounek, M.
Prague-Uhříněves The Institute of Animal Science Prague Czech Republic,
Duskova, D.
Prague-Uhříněves The Institute of Animal Science Prague Czech Republic,
Kowalczyk, J.
Polish Academy of Sciences The Kielanowski Institute of Animal Physiology and Nutrition 05-110 Jabłonna Poland,
Konda, R. K.
Hindu College of Pharmacy Department of Pharmaceutical Chemistry Amaravathi Road Guntur Andhrapradesh 522002 India, victory2ravi@yahoo.co.in,
Chandu, B. R.
Donbosco College of Pharmacy Department of Pharmaceutical Sciences Pulladigunta Guntur 522201 India,
Challa, B. R.
Nirmala College of Pharmacy Department of Pharmaceutical Analysis Madras Road Kadapa Andhrapradesh 516002 India, baluchalla_99@yahoo.com,
Chandrasekhar, K. B.
Jawaharlal Nehru Technological University Department of Chemistry Anantapur 515002 India,
Shen, Y.
Jiangsu University School of Pharmacy Zhenjiang China,
Yang, H.
Jiangsu University School of Pharmacy Zhenjiang China,
Xia, G.
Jiangsu University School of Pharmacy Zhenjiang China,
Wang, J.
Jiangsu University School of Pharmacy Zhenjiang China,
Cai, B.
Nanjing University of Chinese Medicine Jiangsu Key Laboratory of Chinese Medicine Processing, SATCM Key Laboratory of Standardization for Chinese Medicine Processing Nanjing China,
Jia, X.
Jiangsu University School of Pharmacy Zhenjiang China, jxiaobin2005@hotmail.com,
Nurulain, S. M.
United Arab Emirates University Department of Pharmacology and Pharmacotherapy Al Ain United Arab Emirates,
Kalász, H.
Semmelweis University Department of Pharmacology and Pharmacotherapy Budapest Hungary, huba.kalasz@gmail.com,
Szegi, P.
Semmelweis University Department of Pharmacodynamics Budapest Hungary,
Kuca, K.
University Hospital Biomedical Research Center Hradec Kralove Czech Republic,
Adem, A.
United Arab Emirates University Department of Pharmacology and Pharmacotherapy Al Ain United Arab Emirates,
Hasan, M. Y. B.
United Arab Emirates University Department of Pharmacology and Pharmacotherapy Al Ain United Arab Emirates,
Hashemi, F.
Semmelweis University Department of Pharmacodynamics Budapest Hungary,
Tekes, K.
Semmelweis University Department of Pharmacodynamics Budapest Hungary,
Sangoi, M. S.
Federal University of Rio Grande do Sul Faculty of Pharmacy 90610-000 Porto Alegre-RS Brazil, maxsilvasangoi@yahoo.com.br,
Wrasse-Sangoi, M.
Federal University of Santa Maria Department of Industrial Pharmacy 97105-900 Santa Maria-RS Brazil,
Hurtado, F. K.
Federal University of Rio Grande do Sul Faculty of Pharmacy 90610-000 Porto Alegre-RS Brazil,
Rolim, C. M. B.
Federal University of Santa Maria Department of Industrial Pharmacy 97105-900 Santa Maria-RS Brazil,
Walorczyk, S.
Institute of Plant Protection — National Research Institute Department of Pesticide Residue Research Władysława Węgorka 20 60-318 Poznań Poland, s_walorczyk@tlen.pl,
Li, W.
Jilin Agricultural University College of Chinese Medicinal Material Changchun 130118 China,
Shi, L. L.
Jilin Agricultural University College of Chinese Medicinal Material Changchun 130118 China,
Han, L. Q.
Jilin Agricultural University College of Chinese Medicinal Material Changchun 130118 China,
Zhang, J.
Jilin Agricultural University College of Chinese Medicinal Material Changchun 130118 China, zhjing0701@163.com,
Mirjalili, M. H.
Shahid Beheshti University Medicinal Plants and Drugs Research Institute G.C., Evin Tehran Iran,
Navarro, A.
National Autonomous University of Mexico Department of Food and Biotechnology, Faculty of Chemistry Ciudad de México 04510 Mexico,
Hernández, L.
Universidad Autónoma Metropolitana-Xochimilco Departamento de Sistemas Biológicos Calz. del Hueso 1100, Col. Villa Quietud 04960 México, D. F. México,
Jáuregui, O.
Universitat de Barcelona Scientific & Technological Centers (CCiTUB) c/Baldiri i Reixach 10-12 08028 Barcelona Spain,
Bonfill, M.
Universitat de Barcelona Laboratori de Fisiologia Vegetal, Facultat de Farmàcia Avda. Joan XXIII s/n 08028 Barcelona Spain, mbonfill@ub.edu,
Emir, A.
Ege University Department of Pharmacognosy, Faculty of Pharmacy 35100 Bornova, Izmir Turke,
Cicek-Polat, D.
Ege University Department of Pharmacognosy, Faculty of Pharmacy 35100 Bornova, Izmir Turke,
Kaya, G. I.
Ege University Department of Pharmacognosy, Faculty of Pharmacy 35100 Bornova, Izmir Turke,
Sarikaya, B.
Ege University Department of Pharmacognosy, Faculty of Pharmacy 35100 Bornova, Izmir Turke,
Onur, M. A.
Ege University Department of Pharmacognosy, Faculty of Pharmacy 35100 Bornova, Izmir Turke,
Unver-Somer, N.
Ege University Department of Pharmacognosy, Faculty of Pharmacy 35100 Bornova, Izmir Turke,
Abstrakty : Galanthamine, used as a drug for the treatment of Alzheimer’s disease and lycorine possessing important biological properties, are widely distributed alkaloids in the members of Amaryllidaceae family. In the present study, the contents of these alkaloids have been quantitatively determined by high-performance liquid chromatography (HPLC) coupled with diode array detector (DAD) in the aerial parts and bulbs of Galanthus woronowii Losinsk. collected from different localities in Northeastern Turkey. A simple extraction method utilizing columns pre-packed with diatomaceous earth (Extrelut®) was used. Chromatographic separation was achieved using an isocratic system with a mobile phase of trifluoroacetic acid-water-acetonitrile (0.01:90:10) applied at a flow rate of 1 mL min.-1. Validation studies were also carried out to show that the method is specific, accurate, and precise. In the tested specimens, the lycorine content ranged between 0.008 and 0.364% whereas the galanthamine content was found to be between 0.003 and 0.506%.

Słowa kluczowe : alkaloids, Amaryllidaceae, Galanthus, galanthamine, lycorine,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2013
Numer : Vol. 25, no. 4
Strony : 755 – 764
Bibliografia : 1 A.P. Davis, in: M. Bishop, A.P. Davis, and J. Grimshaw (eds) Snowdrops, A Monograph of Cultivated Galanthus, Griffin Press Publishing Ltd., Cheltenham, 2006, pp. 9–63
2 O. Hoshino, in: G.A. Cordell (ed) The Alkaloids Chemistry and Biology, vol. 51, Academic Press Inc., New York, 1998, pp. 323–424
3 N. Unver, Phytochem. Rev., 6, 125 (2007)
4 M. Heinrich, in: G.A. Cordell (ed) The Alkaloids: Chemistry and Biology, vol. 68, Elsevier Inc., San Diego, 2010, pp. 157–165
5 D. Lamoral-Theys, A. Andolfi, G. Van Goietsenoven, A. Cimmino, B. Le Calvé, N. Wauthoz, V. Mégalizzi, T. Gras, C. Bruyére, J. Dubois, V. Mathieu, A. Kornienko, R. Kiss, and A. Evidente, J. Med. Chem., 52, 6244 (2009)
6 S. Li, C. Chen, H. Zhang, H. Guo, H. Wang, L. Wang, X. Zhang, S. Hua, J. Yu, P. Xiao, R. Li, and X. Tan, Antiviral Res., 67, 18 (2005)
7 S. van Dyk, S. Griffiths, R.L. van Zyl, and S.F. Malan, Afr. J. Biotechnol., 8, 5595 (2009)
8 N.R. Mustafa, I.K. Rhee, and R. Verpoorte, J. Liq. Chromatogr. Relat. Technol., 26, 3217 (2003)
9 G.I. Kaya, A. Fillik, Y. Hısıl, and N. Unver, Turkish J. Pharm. Sci., 1, 105 (2004)
10 F.M. Diop, A. Ptak, F. Chretien, M. Henry, Y. Chapleur, and D. Laurain-Mattar, Nat. Prod. Commun., 1, 475 (2006)
11 G.S. Citoglu, B.S. Yılmaz, and O. Bahadır, Chem. Nat. Comp., 44, 826 (2008)
12 G.I. Kaya, D. Cicek, B. Sarıkaya, M.A. Onur, and N. Unver Somer, Nat. Prod. Commun., 5, 873 (2010)
13 S. Berkov, A. Pavlov, M. Ilieva, M. Burrus, S. Popov, and M. Stanilova, Phytochem. Anal., 16, 98 (2005)
14 R. Gotti, J. Fiori, M. Bartolini, and V. Cavrini, J. Pharmaceut. Biomed., 42, 17 (2006)
15 S. Berkov, J. Bastida, F. Viladomat, and C. Codina, Phytochem. Anal., 19, 285 (2008)
16 F. Conforti, M.R. Loizzo, M. Marrelli, F. Menichini, G.A. Statti, D. Uzunov, and F. Menichini, Pharm. Biol., 48, 2 (2010)
17 S. Berkov, J. Bastida, F. Viladomat, and C. Codina, Talanta, 83, 1455 (2011)
18 A.H. Abou-Donia, S.M. Toaima, H.M. Hammoda, and E. Shawky, Phytochem. Anal., 19, 353 (2008)
19 T. Mroczek and J. Mazurek, Anal. Chim. Acta, 633, 188 (2009)
20 A. Lubbe, B. Pomahaćová, Y.H. Choi, and R. Verpoorte, Phytochem. Anal., 21, 66 (2010)
21 Latvala, M.A. Onur, T. Gozler, A. Linden, B. Kivcak, and M. Hesse, Phytochemistry, 39, 1229 (1995)
22 ICH Guidelines Q2 (R1), Validation of Analytical Procedures: Text and Methodology, Geneva, November 2005
23 L. Kintsurashvili and V. Vachnadze, Pharm. Chem. J., 41, 492 (2007)
24 B. Bozkurt Sarıkaya, N. Unver Somer, and M.A. Onur, Turkish J. Pharm. Sci., 9, 61 (2012)
DOI :
Cytuj : Patil, A. N. ,Nirmal, S. A. ,Chavan, A. K. ,Zhou, L. ,Xu, J. ,Luan, L. ,Ma, J. ,Gong, Y. ,Qin, D. ,Pan, C. ,Jinlong, M. ,Guobin, J. ,Bo, J. ,Hua, J. ,Montejo, C. ,Civera, C. ,Barcia, E. ,Negro, S. ,Fernández-Carballido, A. ,Czauderna, M. ,Marounek, M. ,Duskova, D. ,Kowalczyk, J. ,Konda, R. K. ,Chandu, B. R. ,Challa, B. R. ,Chandrasekhar, K. B. ,Shen, Y. ,Yang, H. ,Xia, G. ,Wang, J. ,Cai, B. ,Jia, X. ,Nurulain, S. M. ,Kalász, H. ,Szegi, P. ,Kuca, K. ,Adem, A. ,Hasan, M. Y. B. ,Hashemi, F. ,Tekes, K. ,Sangoi, M. S. ,Wrasse-Sangoi, M. ,Hurtado, F. K. ,Rolim, C. M. B. ,Walorczyk, S. ,Li, W. ,Shi, L. L. ,Han, L. Q. ,Zhang, J. ,Mirjalili, M. H. ,Navarro, A. ,Hernández, L. ,Jáuregui, O. ,Bonfill, M. ,Emir, A. ,Cicek-Polat, D. ,Kaya, G. I. ,Sarikaya, B. ,Onur, M. A. ,Unver-Somer, N. , Simultaneous determination of lycorine and galanthamine in Galanthus woronowii by HPLC-DAD. Acta Chromatographica Vol. 25, no. 4/2013
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Specific mobility of selected phytochemicals in thin-layer chromatographic systems and its possible relevance to pharmacokinetics

Czasopismo : Acta Chromatographica
Tytuł artykułu : Specific mobility of selected phytochemicals in thin-layer chromatographic systems and its possible relevance to pharmacokinetics

Autorzy :
Patil, A. N.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India,
Nirmal, S. A.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India, nirmalsunil@rediffmail.com,
Chavan, A. K.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India,
Zhou, L.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Xu, J.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Luan, L.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Ma, J.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Gong, Y.
Ministry of Agriculture Institute for the Control of Agrochemicals Beijing 100026 China,
Qin, D.
Ministry of Agriculture Institute for the Control of Agrochemicals Beijing 100026 China,
Pan, C.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Jinlong, M.
Zhejiang University Department of Chemical and Biological Engineering Hangzhou 310027 China,
Guobin, J.
Dalian Nationalities University Environment and Resources College Dalian 11660 China,
Bo, J.
Dalian Nationalities University Life Science College Dalian 116600 China,
Hua, J.
Zhejiang University Department of Chemical and Biological Engineering Hangzhou 310027 China,
Montejo, C.
Universidad CEU San Pablo Departamento de Ciencias Farmacéuticas y de la Alimentación, Área de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28668 Madrid Spain, montejo@ceu.es,
Civera, C.
Universidad Complutense de Madrid Departamento de Química Física II, Facultad de Farmacia 28040 Madrid Spain,
Barcia, E.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Negro, S.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Fernández-Carballido, A.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Czauderna, M.
Polish Academy of Sciences The Kielanowski Institute of Animal Physiology and Nutrition 05-110 Jabłonna Poland, mr.czauderna@gmail.com,
Marounek, M.
Prague-Uhříněves The Institute of Animal Science Prague Czech Republic,
Duskova, D.
Prague-Uhříněves The Institute of Animal Science Prague Czech Republic,
Kowalczyk, J.
Polish Academy of Sciences The Kielanowski Institute of Animal Physiology and Nutrition 05-110 Jabłonna Poland,
Konda, R. K.
Hindu College of Pharmacy Department of Pharmaceutical Chemistry Amaravathi Road Guntur Andhrapradesh 522002 India, victory2ravi@yahoo.co.in,
Chandu, B. R.
Donbosco College of Pharmacy Department of Pharmaceutical Sciences Pulladigunta Guntur 522201 India,
Challa, B. R.
Nirmala College of Pharmacy Department of Pharmaceutical Analysis Madras Road Kadapa Andhrapradesh 516002 India, baluchalla_99@yahoo.com,
Chandrasekhar, K. B.
Jawaharlal Nehru Technological University Department of Chemistry Anantapur 515002 India,
Shen, Y.
Jiangsu University School of Pharmacy Zhenjiang China,
Yang, H.
Jiangsu University School of Pharmacy Zhenjiang China,
Xia, G.
Jiangsu University School of Pharmacy Zhenjiang China,
Wang, J.
Jiangsu University School of Pharmacy Zhenjiang China,
Cai, B.
Nanjing University of Chinese Medicine Jiangsu Key Laboratory of Chinese Medicine Processing, SATCM Key Laboratory of Standardization for Chinese Medicine Processing Nanjing China,
Jia, X.
Jiangsu University School of Pharmacy Zhenjiang China, jxiaobin2005@hotmail.com,
Nurulain, S. M.
United Arab Emirates University Department of Pharmacology and Pharmacotherapy Al Ain United Arab Emirates,
Kalász, H.
Semmelweis University Department of Pharmacology and Pharmacotherapy Budapest Hungary, huba.kalasz@gmail.com,
Szegi, P.
Semmelweis University Department of Pharmacodynamics Budapest Hungary,
Kuca, K.
University Hospital Biomedical Research Center Hradec Kralove Czech Republic,
Adem, A.
United Arab Emirates University Department of Pharmacology and Pharmacotherapy Al Ain United Arab Emirates,
Hasan, M. Y. B.
United Arab Emirates University Department of Pharmacology and Pharmacotherapy Al Ain United Arab Emirates,
Hashemi, F.
Semmelweis University Department of Pharmacodynamics Budapest Hungary,
Tekes, K.
Semmelweis University Department of Pharmacodynamics Budapest Hungary,
Sangoi, M. S.
Federal University of Rio Grande do Sul Faculty of Pharmacy 90610-000 Porto Alegre-RS Brazil, maxsilvasangoi@yahoo.com.br,
Wrasse-Sangoi, M.
Federal University of Santa Maria Department of Industrial Pharmacy 97105-900 Santa Maria-RS Brazil,
Hurtado, F. K.
Federal University of Rio Grande do Sul Faculty of Pharmacy 90610-000 Porto Alegre-RS Brazil,
Rolim, C. M. B.
Federal University of Santa Maria Department of Industrial Pharmacy 97105-900 Santa Maria-RS Brazil,
Walorczyk, S.
Institute of Plant Protection — National Research Institute Department of Pesticide Residue Research Władysława Węgorka 20 60-318 Poznań Poland, s_walorczyk@tlen.pl,
Li, W.
Jilin Agricultural University College of Chinese Medicinal Material Changchun 130118 China,
Shi, L. L.
Jilin Agricultural University College of Chinese Medicinal Material Changchun 130118 China,
Han, L. Q.
Jilin Agricultural University College of Chinese Medicinal Material Changchun 130118 China,
Zhang, J.
Jilin Agricultural University College of Chinese Medicinal Material Changchun 130118 China, zhjing0701@163.com,
Mirjalili, M. H.
Shahid Beheshti University Medicinal Plants and Drugs Research Institute G.C., Evin Tehran Iran,
Navarro, A.
National Autonomous University of Mexico Department of Food and Biotechnology, Faculty of Chemistry Ciudad de México 04510 Mexico,
Hernández, L.
Universidad Autónoma Metropolitana-Xochimilco Departamento de Sistemas Biológicos Calz. del Hueso 1100, Col. Villa Quietud 04960 México, D. F. México,
Jáuregui, O.
Universitat de Barcelona Scientific & Technological Centers (CCiTUB) c/Baldiri i Reixach 10-12 08028 Barcelona Spain,
Bonfill, M.
Universitat de Barcelona Laboratori de Fisiologia Vegetal, Facultat de Farmàcia Avda. Joan XXIII s/n 08028 Barcelona Spain, mbonfill@ub.edu,
Emir, A.
Ege University Department of Pharmacognosy, Faculty of Pharmacy 35100 Bornova, Izmir Turke,
Cicek-Polat, D.
Ege University Department of Pharmacognosy, Faculty of Pharmacy 35100 Bornova, Izmir Turke,
Kaya, G. I.
Ege University Department of Pharmacognosy, Faculty of Pharmacy 35100 Bornova, Izmir Turke,
Sarikaya, B.
Ege University Department of Pharmacognosy, Faculty of Pharmacy 35100 Bornova, Izmir Turke,
Onur, M. A.
Ege University Department of Pharmacognosy, Faculty of Pharmacy 35100 Bornova, Izmir Turke,
Unver-Somer, N.
Ege University Department of Pharmacognosy, Faculty of Pharmacy 35100 Bornova, Izmir Turke,
Knaś, M.
University of Silesia Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Szeremeta, D.
University of Silesia Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Polański, J.
University of Silesia Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Sajewicz, M.
University of Silesia Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Kowalska, T.
University of Silesia Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland, teresa.kowalska@us.edu.pl,
Abstrakty : In many publications on the thin-layer chromatographic analysis of the medicinal plant extracts, striking skewness of the separated chromatographic bands with many phytochemicals can be seen, although no attention has ever been paid to this odd mass transfer effect. In our earlier studies on the thin-layer chromatographic enantioseparation of certain low molecular weight carboxylic acids, lateral relocation thereof (i.e., the side-wise deviation of their migration route from linearity) was registered. Then we found out that lateral relocation was observed with these analytes only, which structurally resembled molecular rotors. In this study, we investigate the role played by the thin-layer chromatographic stationary phases in lateral relocation of botanically relevant molecular rotors. Thus, three carboxylic acids were selected as the test analytes, all of them resembling molecular rotors and abundantly present in the medicinal plant extracts. We selected two most popular thin-layer chromatographic stationary phases also, i.e., silica gel (characterizing with microcrystalline chirality) and alumina (achiral). Lateral relocation of the test analytes was observed on the silica gel stationary phase only. A conclusion was drawn that the chiral stationary phase makes a complementary contribution to lateral relocation (along with the propeller-like molecular structure of the analytes). In our view, specially devised thin-layer chromatographic systems can prove a convenient nano-platform for future investigation of the drug transport patterns, advantageous in the pharmacokinetic studies.

Słowa kluczowe : molecular rotors, chirality, mass transfer in TLC, “asymmetric receptors” model, pharmacokinetics,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2013
Numer : Vol. 25, no. 4
Strony : 765 – 776
Bibliografia : 1 P. Pachaly and A. Koch, DC-Atlas Dünnschichtchromatographie in der Apotheke, Wissenschaftliche Verlagsgesellschaft Stuttgart, Stuttgart, 2012
2 Wagner and S. Bladt, Plant Drug Analysis, A Thin Layer Chromatography Atlas, 2nd Edition, Springer Verlag, Heidelberg, 2009
3 Thin Layer Chromatography in Phytochemistry. M. Waksmundzka-Hajnos, J. Sherma, and T. Kowalska (Eds), CRC Press/Taylor & Francis Group, Boca Raton, FL, 2008
4 M. Sajewicz, H.E. Hauck, G. Drabik, E. Namysło, B. Głód, and T. Kowalska, J. Planar Chromatogr. — Modern TLC, 19, 278 (2006)
5 M. Sajewicz, G. Grygierczyk, M. Gontarska, and T. Kowalska, J. Liq. Chromatogr. Relat. Technol., 30, 2185 (2007)
6 M. Sajewicz, M. Gontarska, A. Dąbrowa, and T. Kowalska, J. Liq. Chromatogr. Relat. Technol., 30, 2369 (2007)
7 Polański, M. Sajewicz, M. Knaś, M. Gontarska, and T. Kowalska, J. Planar Chromatogr. — Modern TLC, 25, 208 (2012)
8 Polański, M. Sajewicz, M. Knaś, M.A. Żywociński, M. Weloe, and T. Kowalska, Chimica Oggi — Chemistry Today, Monographic Supplement Series “Chiral Technologies,” 30(5), 18 (2012)
9 Polański, M. Sajewicz, M. Knaś, A. Żywociński, M. Weloe, and T. Kowalska, Vestnik St. Petersburg University (in press)
10 H.A.G. Niederlander, T.A. Van Beek, A. Bartasiute, and I.I. Koleva, J Chromatogr A, 1210, 121 (2008)
DOI :
Cytuj : Patil, A. N. ,Nirmal, S. A. ,Chavan, A. K. ,Zhou, L. ,Xu, J. ,Luan, L. ,Ma, J. ,Gong, Y. ,Qin, D. ,Pan, C. ,Jinlong, M. ,Guobin, J. ,Bo, J. ,Hua, J. ,Montejo, C. ,Civera, C. ,Barcia, E. ,Negro, S. ,Fernández-Carballido, A. ,Czauderna, M. ,Marounek, M. ,Duskova, D. ,Kowalczyk, J. ,Konda, R. K. ,Chandu, B. R. ,Challa, B. R. ,Chandrasekhar, K. B. ,Shen, Y. ,Yang, H. ,Xia, G. ,Wang, J. ,Cai, B. ,Jia, X. ,Nurulain, S. M. ,Kalász, H. ,Szegi, P. ,Kuca, K. ,Adem, A. ,Hasan, M. Y. B. ,Hashemi, F. ,Tekes, K. ,Sangoi, M. S. ,Wrasse-Sangoi, M. ,Hurtado, F. K. ,Rolim, C. M. B. ,Walorczyk, S. ,Li, W. ,Shi, L. L. ,Han, L. Q. ,Zhang, J. ,Mirjalili, M. H. ,Navarro, A. ,Hernández, L. ,Jáuregui, O. ,Bonfill, M. ,Emir, A. ,Cicek-Polat, D. ,Kaya, G. I. ,Sarikaya, B. ,Onur, M. A. ,Unver-Somer, N. ,Knaś, M. ,Szeremeta, D. ,Polański, J. ,Sajewicz, M. ,Kowalska, T. , Specific mobility of selected phytochemicals in thin-layer chromatographic systems and its possible relevance to pharmacokinetics. Acta Chromatographica Vol. 25, no. 4/2013
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